Short Communication: Genetic variation of Coelogyne pandurate, C. rumphii and their hybrids based on RAPD markers
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Abstract. Hartati S, Muliawati ES. 2020. Short Communication: Genetic variation of Coelogyne pandurata, C. rumphii and their hybrids based on RAPD markers. Biodiversitas 21: 4709-4713. One effort to increase the genetic diversity of orchids is by crossing. This research aims to assess the genetic variation of a hybrid orchid obtained by crossing Coelogyne pandurata and C. rumphii and their hybrids based on RAPD markers. In this research, both parents were analyzed in three replications, while the hybrid was done in 10 replications. The study was conducted by analyzing DNA bands using RAPD markers with six primers, i.e. OPA 02, OPA 07, OPA 13, OPB 12, OPB 17, and OPD 08. Identification of the parents and their F1 hybrids showed 95.83% polymorphic bands with 43 bands measuring 200-2100 bp. The parents of C. rumphii, C. pandurata, and their hybrids showed similarity range of 0.16-1.00. The crossing of C. rumphii and C. pandurata resulted in a similarity of 0.5, shown in two large clusters. The first cluster consisted only of C. pandurata males and the second cluster consisted of C. rumphii females, together with all hybrid individuals. This study succeeded in creating new hybrids of orchids that have different characters from their parents, having a genetic variation of 23%.
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References
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Hartati S., E.S Muliawati, Pardono, O Cahyono, P Yuliyanto. 2019a. Morphological characterization of Coelogyne spp for germplasm conservation of orchids. Rev. Ceres, Viçosa, v. 66, n.4, p. 265-270,
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Niknejad A, Kadir MA, Kadzimin SB, Abdullah NAP, Sorkheh K (2009). Moleculer characterization and phylogenetic relationship among and within species of Phalaenopsis (Epdendroideae: Orchidaceae) base on RAPD analysis. Africa Journal Biotechnology 8: 5225-5240
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Pharmawati M and Macfarlane I.J. 2013. The Genetics Relationships of Grevillea Hybrids Determined by RAPD Marker. HAYATI Journal of Biosciences. 20 (4): 196-200
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Sulistianingsih R, Semiarti E, Purwantoro A., Mangoendidjojo. 2010. Analisis keragaman genetic mutan anggrek Phalaenopsis amabilis L, blume dengan RAPD. Seminar Nasional Biologi UGM Jogyakarta.
Susantidiana, Wijaya A, Lakitan B dan Surahman M. 2009. The Identification of Some Accessions of Jatropha curcas L.Using Morphological and RAPD Analysis. Jurnal Agronomi Indonesia, 37(2): 167-173.
Tanee Tawatchai Piyawadee Chadmuk, Runglawan Sudmoon, Arunrat Chaveerach and Kowit Noikotr, 2012. Genetic analysis for identification, genomic template stability in hybrids and barcodes of the Vanda species (Orchidaceae) of Thailand. African Journal of Biotechnology Vol. 11(55), pp. 11772-11781
Williams JG, Kubelik AR, Livak KJ, Rafalsky JA, Tingev SV (1990) DNA plolymorphism amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18: 6531-6535.
Xiang N, Hong Y, and Lam-Chan LT. 2003. Genetic Analysis of Tropical Hybrids (Dendrobium) with Fluorescence Amplified Fragment-length Polymorphism (AFLP). J. Amer. Soc. Hort. Sci. 128 (5): 731-735
Yuping Z, Wang W, Li X dan Zhao X. 2012. Study on Interspecific Compatibility of Different Combinations Inner (Inter) Lily Hybrids. Journal of Scientific Research 11 (5): 567-574
Zhang H.Y., Li F.S., He I.L., Zhong H.Q., Yang Q.H., and He S.C. 2009. Identification of sugarcane interspecies hybrids with RAPDs. African Journal of Biotechnology 7 (5): 1072-1074.
Chang Y.K, R.E Veilleux, and M.J Iqbal 2009. Analysis of Genetics Variability among Phalaenopsis Species and Hybrids Using Amplified Fragment Length Polymorphism. J. Amer. Soc. Hort. Sci. 134 (1): 58-66
Chung S.Y and S.H Choi. 2012. Genetic Variability and Relationships Among Interspecific Hybrid Cultivars and Parental Species of Paphiopedilum Via Ribosomal DNA Sequence Analysis. Plant Syst Evol: 1897-1907. DOI 10.1007/s00606-012-0689-2
Devi B,C., Sahaya Shibu.B, and Servin Wesly P, 2012. In vitro Regeneration of Coelogyne stricta Direct Somatic Embryogenesis . Journal Trop. Med. Plants Vol.13 No.2
Gregor, Mc., C.E., C.A. Lambert, M.M. Grylic, J.H. Louw, and L. Warnich. 2000. A comparison assessment of DNA finger printing technique (RAPD, ISSR, AFLP, and SSR) in tetraploid potato (Solanum tuberosum L.) germplasma. Euphytica 113 : 135 - 144.
Hartati S. 2017. Study of Genetic Diversity on Six Species of Indonesian Coelogyne spp. Based on ISSR Markers. Pakistan Journal of Biological Sciences. 20 (11): 577 – 583. ISSN 1028-8880. DOI: 10.3923/pjbs.2017.577-583.
Hartati S., E.S Muliawati, Pardono, O Cahyono, P Yuliyanto. 2019a. Morphological characterization of Coelogyne spp for germplasm conservation of orchids. Rev. Ceres, Viçosa, v. 66, n.4, p. 265-270,
Hartati S., Nandariyah, A Yunus, D.W Djoar. 2019b. Hybridization technique of black orchid (Coelogyne pandurata Lindley) to enrich the genetic diversity and to rescue the genetic extinction. Bulgarian Journal of Agricultural Science, 25 (No 4) 2019, 751–755.
Inthawong S, Weenun B, Nuttha K and Pimchai A. 2006. Analysis of Intersectional Hybrid of Dendrobium by RAPD Technique. Kasetsart Journal, 40(2):456-461.
Julisaniah, N.I., Sulistyowati, L., dan Sugiharto, A.N. 2008. Analisis Kekerabatan Mentimun (Cucumis sativus L.) menggunakan Metode RAPD-PCR dan Isozim. Jurnal Biodiversitas 9(2) : 99–102.
Khisor R and GJ Sharma 2008 Intergeneric hybrid of two rare and endangered orchids, Renanthera imschootiana Rolfe and Vanda coerulea Griff. ex L. (Orchidaceae): Synthesis and characterization. Euphytica 165(2):247-256.DOI: 10.1007/s10681-008-9755-9
Khosravi, A.R., Kadir M.A, Kadzemin S.B, Zaman F.Q, And De Silva A.E. 2009. RAPD Analysis Of Cholchicine Induced Variation Of The Dendrobium Serdang Beauty. African Journal Of Biotechnology 8 (8):1455-1465
Kusumadewi SYdan Mansur M. 2012. The Occurrence of Hybrid in Nepenthes hookeriana Lindl. From Central Kalimantan can be Detected by RAPD and ISSR Markers, HAYATI Journal of Biosciences, 19(1): 18-24.
Nielsen LR, H.R Siegismund (1999) Interspecific differentiation and hybridization in Vanilla species (Orchidaceae). Heredity 83:560–567
Niknejad A, Kadir MA, Kadzimin SB, Abdullah NAP, Sorkheh K (2009). Moleculer characterization and phylogenetic relationship among and within species of Phalaenopsis (Epdendroideae: Orchidaceae) base on RAPD analysis. Africa Journal Biotechnology 8: 5225-5240
Parab, G.V and Krishnan S. 2008. Assessment Of Genetic Variation Among Populations of Rhynchostylis retusa an Epiphytic Orchid From Goa, India Using ISSR And RAPD Marker. Journal of Biotechnology, 7(17): 313-319.
Pharmawati M and Macfarlane I.J. 2013. The Genetics Relationships of Grevillea Hybrids Determined by RAPD Marker. HAYATI Journal of Biosciences. 20 (4): 196-200
Poerba, Y.S and F. Ahmad, 2013. Analisis keragaman genetic Musa Balbisiana colla berdasarkan marka RAPD dan ISSR [Genetic variation analyses of Musa balbisiana Colla based on RAPD and ISSR markers]. Berita Biol. 12:259-267.
Poerba, Y. S. and D Martanti. 2008. Keragaman Genetik Berdasarkan Marka Random Amplified Polymorphic DNA pada Amorphopallus muelleri Blume di Jawa. Jurnal Biodiversitas 9(4) : 245 – 249.
Rohlf. 1998. NTSYS-pc Numerical Taxonomy and Multivariative Analysis Syersion Version 2,02. Exerter sorfware. New York.
Sivanaswari, Chalaparmal, Thohirah, LA , Fadelah, AA , dan Abdullah, NAP. 2011. Hybridization of several Aerides species and in vitro germination of its hybrid. African Journal of Biotechnology, 10(53): 10864-10870.
Sulistianingsih R, Semiarti E, Purwantoro A., Mangoendidjojo. 2010. Analisis keragaman genetic mutan anggrek Phalaenopsis amabilis L, blume dengan RAPD. Seminar Nasional Biologi UGM Jogyakarta.
Susantidiana, Wijaya A, Lakitan B dan Surahman M. 2009. The Identification of Some Accessions of Jatropha curcas L.Using Morphological and RAPD Analysis. Jurnal Agronomi Indonesia, 37(2): 167-173.
Tanee Tawatchai Piyawadee Chadmuk, Runglawan Sudmoon, Arunrat Chaveerach and Kowit Noikotr, 2012. Genetic analysis for identification, genomic template stability in hybrids and barcodes of the Vanda species (Orchidaceae) of Thailand. African Journal of Biotechnology Vol. 11(55), pp. 11772-11781
Williams JG, Kubelik AR, Livak KJ, Rafalsky JA, Tingev SV (1990) DNA plolymorphism amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18: 6531-6535.
Xiang N, Hong Y, and Lam-Chan LT. 2003. Genetic Analysis of Tropical Hybrids (Dendrobium) with Fluorescence Amplified Fragment-length Polymorphism (AFLP). J. Amer. Soc. Hort. Sci. 128 (5): 731-735
Yuping Z, Wang W, Li X dan Zhao X. 2012. Study on Interspecific Compatibility of Different Combinations Inner (Inter) Lily Hybrids. Journal of Scientific Research 11 (5): 567-574
Zhang H.Y., Li F.S., He I.L., Zhong H.Q., Yang Q.H., and He S.C. 2009. Identification of sugarcane interspecies hybrids with RAPDs. African Journal of Biotechnology 7 (5): 1072-1074.
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