Abstract. Ratnasari A, Jabal AR, Syahribulan, Idris I, Rahma N, Rustam SNRN, Karmila M, Hasan H, Wahid I. 2021. Salinity tolerance of larvae Aedes aegypti inland and coastal habitats in Pasangkayu, West Sulawesi, Indonesia. Biodiversitas 22: 1203-1210. Aedes aegypti L. is the primary arboviral vector generally found in freshwater, but it is also observed in brackish water. The study analyzes habitat characteristics, larvae adaptation, and oviposition preference in salinity levels over the coastal and inland ecosystem in Pasangkayu District of West Sulawesi. The larvae were reared until adults and have larval of first progeny were examined the salinity tolerance in five salinity levels. Ovitrap-based experiments were carried out in the laboratory using adult Ae. aegypti colonies from inland and coastal areas. Eggs on filter paper (ovitrap) were identified and counted. Data were analyzed using LC50 (larvae with salinity tolerance) and Pearson correlation (correlation between the larvae phase two ecosystem factors). Total larvae observed in coastal areas and inland are 1437 and 1288, respectively. The salinity tolerance test showed that Instar IV is highly adapted to the saline environment. The larvae from the coastal (inland) area can tolerate salinity up to 13 (10) ppt. Mosquitoes can lay many eggs in 3 ppt salinity: 27.1% and 20.8% for coastal and inland colonies, respectively. Pearson correlation analysis showed a significant correlation between the larval instar stage of Ae. aegypti from coastal and inland ecosystems with the ability to survive at the salinity level (p < 0.01). This study is expected to be a source of information on the adaptation of Ae. aegypti mosquito to salinity in coastal and inland ecosystems. The findings can be considered in vector control efforts on brackish water habitats.
Augustina I, Jabal AR, Permana GI, Ratnasari AR. 2020. Distribution and ecology of mosquito larvae in Pahandut Sub-District Palangkaraya City. J of Physics : Conference series ICMSE 2020 [in the publish stage]
Bradley TJ. 1987. Physiology of osmoregulation in mosquitoes. Ann Rev Entomol 32: 439–462.
Clark TM, Flis BJ, Remold SK (2004) Difference in the effects of salinity on larval growth and developmental programs of a freshwater and a euryhaline mosquito species (Insecta: Diptera, Culicidae). J Exp Biol 207: 2289–2295.
Edwards HA. 1982a. Aedes aegypti : energetic of osmoregulation. J Exp Biol 101 : 135-41.
Edwards HA. 1982b. Ion concentrationan and activity in the haemolyph of Aedes aegypti larvae. J Exp Biol 101:143-51.
Ferede G, Tiruneh M, Abate E et al. 2018. Distribution and larval breeding habitats of Aedes mosquito species in residential areas of northwest Ethiopia. Epidemiol Health. Doi: 10.4178/epih.e2018015.
Hemme RR, Tank JL, Chadee DD, Severson DW. 2009. Environmental conditions in water storage drums and influences on Aedes aegypti in Trinidad, West Indies. Acta Trop 112 (1): 59-66. Doi: 10.1016/j.actatropica.2009.06.008.
Hiscock KM, Rivett MO, Davidson RM. 2002. Sustainable groundwater development. Geological Society London Special Publications 193(1):1-14. Doi : 10.1144/GSL.SP.2002.193.01.01
Idris FHJ, Usman A, Surendran SN et al. 2013. Detection of Aedes albopictus pre-imaginal stages in brackish water habitats in Brunei Darussalam. J Vect Ecol 38: 197–199.
Jude PJ, Dharshini S, Vinobaba M et al . 2010. Anopheles culicifacies breeding in brackish waters in Sri Lanka and implications for malaria control. J Malar 9: 106.
Jude PJ, Thamasegaram T, Sivasubramanyam G et al. 2012. Salinity-tolerant larvae of mosquito vectors in the tropical coast of Jaffna, Sri Lanka and the effect of salinity on the toxicity of Bacillus thuringiensis to Aedes aegypti larvae. Parasit Vect 5:269. Doi: 10.1186/1756-3305-5-269.
Kengne P, Charmantier G, Bidet EB et al. 2019. Tolerance of disease-vector mosquitoes to brackish water and their osmoregulatory ability. Ecosphere. Doi : 10.1002/ecs2.2783.
Le Coupanec, A., Tchankouo-Nguetcheu, S., Roux, P., Khun, H., Huerre, M., Morales- Vargas, R., Enguehard, M., Lavillette, D., Missé, D., Choumet, V., 2017. Co-infection of mosquitoes with chikungunya and dengue viruses reveals modulation of the replication of both viruses in midguts and salivary glands of Aedes aegypti mosquitoes. Int. J. Mol. Sci. 18, 1708.
Lincoln RJ, Boxshall GA, Clark PF. 1982. A Dictionary of Ecology, Evolution amd Systematics, Cambridge University Press.
Midega JT, Nzovu J, Kahindi S, Sang RC, Mbogo C. 2006. Application of the pupal/demographic-survey methodology to identify the key container habitats of Aedes aegypti (L.) in Malindi District, Kenya. Ann Trop Med Parasitol 100: 61-72. Doi: 10.1179/136485906X105525.
Ministry of Health Indonesia. 2017. Guidelines for Dengue Fever Entomology Survey and Key to Identification of Aedes Mosquitoes. Ministry of Health, Jakarta. [Indonesian]
Mosha FW, Mutero CM. 1982. The influence of salinity on larval development and population dynamics of Annopeles merus Donitz (Diptera : Culicidae). Bull Entomol Res 72 : 119-28.
Mukhopadhyay AK, Tamizharasu W, Satya BP et al. 2010. Effect of common salt on laboratory reared immature stages of Aedes aegypti (L). Asian Pacific Journal of Tropical Medicine 173-175.
Ngugi HN, Mutuku FM, Ndenga BA, Musunzaji SP, Mbakaya JO, Aswani P, Irungu LW, Mukoko D, Vulule J, Kitron U, LaBeaud AD. 2017. Characterisation and productivity profiles of Aedes aegypti (L.) breeding habitats across rural and urban landscapes in western and coastal Kenya. Parasites Vectors 10 (1): 1-12. Doi: 10.1186/s13071-017-2271-9.
Nicholls RJ, Wong PP, Burkett VR et al. 2007. Coastal systems and low-lying areas. In: Parry ML, Canziani OF, Palutikof JP, van der Linden PJ, Hanson CE, eds. Climate Change 2007: Impacts, Adaptation and Vulnerability, Contribution of Working Group II to the Fourth Assessment Report of the Intergovernmental Panel on Climate Change. Cambridge University Press, Cambridge. pp 315–356
O’Meara GF, Evans DG. 1976. The influence of mating on autogenous egg development in the mosquito Aedes taeniorhynchus. J Insect Physiol 22 : 613-17.
Panigrahi SK, Barik TK, Mohanty S et al. 2014. Laboratory Evaluation of Oviposition Behavior of Field Collected Aedes Mosquitoes. J of Insect. Doi : 10.1155/2014/207489
Pfeffer M, Dobler G. 2010. Emergence of zoonotic arboviruses by animal trade and migration. J Parasit Vectors 3: 35.
Rahma N, Hasan H, Ratnasari A et al. 2020. The application of novel methods of Animal Barrier Screen and Kelambu Trap for mosquitoe’s surveillance in South and West Sulawesi, Indonesia. J Biodiversitas 21: 4787-4794.
Ramasamy R, Surendran SN. 2011. Possible impact of rising sea levels on vector-borne infectious diseases. BMC Infect Dis 11: 18.
Ramasamy R, Surendran SN, Jude PJ et al. 2011. Larval development of Aedes aegypti and Aedes albopictus in peri-urban brackish water and its implications for transmission of arboviral diseases. PLoS NTDs 5 (11): e1369. DOI: 10.1371/journal.pntd.0001369
Ramasamy R, Surendran SN. 2012. Global climate change and its potential impact on disease transmission by salinity-tolerant mosquito vectors in coastal zones. Front Physiol 3: 198. Doi: 10.3389/fphys.2012.00198.
Ramasamy R, Jude PJ, Veluppillai T et al. 2014. Biological Differences between Brackish and Fresh Water-Derived Aedes aegypti from Two Locations in the Jaffna Peninsula of Sri Lanka and the Implications for Arboviral Disease Transmission. PLOS ONE 9 : 8.
Ramasamy R, Surendran SN. 2016. Mosquito vectors developing in atypical anthropogenic habitats—global overview of recent observations mechanisms and impact on disease transmission. J Vector Borne Dis 53 : 91–8.
Ratnasari A, Jabal AR, Wahid I et al. 2020. The ecology of Aedes aegypti and Aedes albopictus larvae habitat in coastal areas of South Sulawesi, Indonesia. J Biodiversitas 21: 4648-4654.
Rezza G, Nicoletti L, Angelini R et al. 2007. Infection with chikungunya virus in Italy: an outbreak in a temperate region. Lancet 370: 1840–1846.
Richards AG, and Meier TJ. 1974. The osmolarity of the blood of a mosquito larvae (Aedes aegypti L.) reared under several different culture conditions. Ann Entomol Soc Am 67 : 424-6.
Rueda LM. 2008. Freshwater Animal Diversity Assessment. DOI: 10.1007/978-1-4020-8259-7.
Sasmono RT, Wahid I, Trimarsanto H, Yohan B, Wahyuni S, Hertanto M, Yusuf I, Mubin H, Ganda IJ, Latief R, Bifani PJ, Shi PY, Schreiber MJ. 2015. Genomic analysis and growth characteristic of dengue viruses from Makassar, Indonesia. Infect Genet Evol 32: 165-177. DOI: 10.1016/j.meegid.2015.03.006.
Surendran SN, Kajatheepan A, Sanjeefkumar KF et al. 2007. Seasonality and insecticide susceptibility of dengue vectors : An ovitraps based survey in a residential area of northern Sri Lanka. Southeast Asian J Trop Med Public Health 38 : 276–282.
Surendran SN, Ramasamy R 2010. The Anopheles culicifacies and Anopheles subpictus species complexes in Sri Lanka and their implications for malaria control in the country. Trop Med Health 38: 1–11.
Surendran SN, Jude PJ, Ramasamy R et al. 2011. Variations in salinity tolerance of malaria vectors of the Anopheles subpictus complex in Sri Lanka and the implications for malaria transmission. Parasit Vectors 4: 117.
Surendran SN, Jude PJ, Thabothiny V et al. 2012. Pre-imaginal development of Aedes aegypti in brackish and fresh water urban domestic wells in Sri Lanka. J Vect Ecol 37(2): 471–473.
Surendran SN, Sivabalakrishnan K, Jayadas TTP et al. 2018. Adaptation of Aedes aegypti to salinity: Characterized by larger anal papillae in larvae. J Vector Borne Dis 55 : 235–238.
Soni M, Khan AS, Bhattacharjee CK, et al. 2020. Experimental study of dengue virus infection in Aedes aegypti and Aedes albopictus : A comparative analysis on susceptibility, virus transmission and reproductive success. J of Invertebrate Pathology 175, 107445.
Souza NJA, Powell JR, Bonizzoni M. 2019. Aedes aegypti vector competence studies: A review. Infect Genet Evol 67: 191-209. DOI: 10.1016/j.meegid.2018.11.009.
Tedjou AN, Kamgang, B, Yougang AP, Njiokou F, Wondji CS. 2018. Update on the geographical distribution and prevalence of Aedes aegypti and Aedes albopictus (Diptera: Culicidae), two major arbovirus vectors in Cameroon. PLoS NTDs 13 (3): 1-18. DOI: 10.1371/journal.pntd.0007137.
Wheeler MW, Park RM, Bailer J. 2009. Comparing median lethal concentration values using confidence interval overlap or ratio tests. J Environmental Toxicology and Chemistry. Vol. 25 : 1441–1444.
Wigglessworth VB. 1933. The adaptation of mosquito larvae to salt water. J Exp Biol 10 : 27-37.
Yap HH, Lee CY, Chong NL et al. 1995. Oviposition site preference of Aedes albopictus in the laboratory. J Am Mosq Control Assoc 11: 128–132.
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