The effect of Averrhoa bilimbi stem extract on the blood glucose level of white rats induced by alloxan

Abstract

Agriani CN, Kisrini, Dharmawan R. 2016. The effect of Averrhoa bilimbi stem extract on the blood glucose level of white rats induced by alloxan. Biofarmasi 14: 56-62. The purpose of this research is to find out the effect of belimbing wuluh stem (Averrhoa bilimbi L.) extract due to the blood glucose level in white rats (Rattus norvegicus) induced by alloxan. This research is a laboratory experiment using experimental control group pre-post test design. Subjects of this research are 25 white male rats (Rattus norvegicus), 2 months of age and 200 g of body weight. Subjects were divided into five groups. All groups were induced by 25 mg/200 g body weight/day of alloxan for three days. On the day of fourth, blood sample was collected from orbital vein of white rats for the measurement of the first blood glucose level (T1). Furthermore, the positive control group received 12,6 mg/200 g body weight/day of metformin, then the first, second, and third group received of each 25, 50, and 100 mg/200 g body weight/day of belimbing wuluh stem extract. On the fifteenth day of treatment, blood samples were collected again from the orbital vein of white rats for the measurement of the second blood glucose level (T2). The measurement of blood glucose was using spectrophotometer with the Glucose GOD PAP method. Then the data were analyzed by using One way ANOVA. Blood glucose rate after induction has subtracted from blood glucose rate after drug given. After that the data would be change in to mean data that represents as: negative control group = 24,4; positive control group 103,4; first given drug group = 83,4; second given drug group = 102,4; and third given drug group = 102,2 (in mg/100 mL unit). The statistical analysis by using One way ANOVA shows a significant difference in blood glucose level among the five groups of treatment with p-value = 0,000. Post Hoc Test shows that the first, second, and third group have the same effectively as the positive control group to decrease the blood glucose level with p > 0,05.