Extraction and assessment of pectin from pumpkin peels
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Abstract
Abstract. Hamed AAR, Mustafa SE. 2018. Extraction and assessment of pectin from pumpkin peels. Biofarmasi J Nat Prod Biochem 16: 1-7. Pectin is characterized as intricate blends of polysaccharides that compose around 33% of most plants’ cell wall dry substance. This study was done to extricate pectin from a completely ripened pumpkin (Cucurbita spp.) using two distinct techniques; the soxhlet acid extraction technique and acid extraction technique, and to find out the impact of utilizing distinctive acids on the yield of pectin; nitric and citric acids were utilized. In addition, to analyze the impact of time on pectin yield, the extraction procedure was carried out using 3 different times, namely, 30, 60, and 90 min. The chemical substances of pumpkin peel (dampness, ash, protein, fat, fiber, total sugar, and calcium) and the chemical properties of pectin (methoxyl content, acetyl content, identical weight, and level of esterification) were extracted with both nitric and citric acids were also identified. The outcomes produced by the soxhlet acid extraction technique and acid extraction technique were 7.72% nitric acid and 6.80% citric acid and 6.24% nitric acid, and 5.36% citric acid, respectively, demonstrating that the utilization of soxhlet acid extraction technique with nitric acid got the highest yield of pectin. Additionally, it was also discovered that the time for pectin extraction was 60 min at the very most. The outcome for the chemical substances of pumpkin peel (dampness, fiery remains, protein, fiber, fats, add up to sugar, and calcium) were 20.1, 7.1, 3.2, 10.15, 2.3, 57.15, and 0.308%, respectively. The examination discovered that the chemical properties of pectin extracted with both nitric and citric acid were for the equivalent weight (1250 and 1250 g/mol), methoxyl content (6.20 and 6.29%), acetyl contains (0.43 and 0.43%) and the level of esterification (66.53 and 66.57%) respectively. The outcome got from this examination showed that pectin extracted from pumpkin peel is of high quantity and quality and is promising for commercial production.
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Concentrates [Thesis]. University of Khartoum. Sudan.
Ali SM, Md Jiaur M. 2014. Isolation and Characterization of Pectin
Extracted from Lemon Pomace during Ripening. J Food Nutr Sci 2
(2): 30-35.
AOAC. 1995. Official Methods of Analysis of the Association of Official
Analytical Chemistry. 16th ed. AOAC International, Washington
D.C., USA.
AOAC. 1999. Association of official Analytical chemists. 16th Edition,
Association of Official Analytical Chemists, Maryland.
Benjamin O, Silcock P, Leus M, Everett D W. 2012. Multilayer emulsions
delivery systems for controlled release of volatile compounds
using pH and salt triggers. Food Hydrocoll 27 (1): 109-118.
Bottger I. 1990. Pectin application—Some practical problems. In: Phillips
GO, Wedlock DJ, Williams PA. (eds.). Gums and Stabilisers for the
Food Industry. IRL Press, Oxford, UK.
Campbell M. 2006. Extraction of Pectin from Watermelon Rind.
Oklahoma State University, Stillwater, Oklahoma
Constenla D, Lozano JE. 2003. Kinetic model of pectin demethylation.
Latin American Appl Res 33: 91-96.
Crandall PG, Braddock RJ, Rouse AH. 1978. Determining the yield and
quality of pectin from fresh peel and pectin pomace. Proc Florida
State Hortic Soc 91: 109-11.
Elmer, MM. 1993. Characterization of apple fruit pectic substances. J Sci
Technol 30 (2): 17-19.
Elshafie, AS. 1981. Isolation, Fractionation, and Characterization of
Pumpkin Pectic Substance. [Thesis]. University of Khartoum, Sudan.
Eltinay AH, El Shafie AS, Nour AM. 1982. A chemical study of Pumpkin
pectic substances. Faculty of Agriculture, University of Khartoum,
Sudan.
Ibrahim, YA. 2008. Isolation and Characterization of Pectic Substance of
pumpkin peels. [Thesis]. University of Khartoum, Sudan.
Ismail NSM, Ramli N, Hani NM, Meon Z. 2012. Extraction and
characterization of pectin from dragon fruit (Hylocereus polyrhizus)
using various extraction conditions. Sains Malaysiana 41: 41-45.
Koubla BB, Mbome TI, Kansci G, Tchouanguaeb Mbiapo F, Crepeau MJ,
Thibault JF, Kallet MC. 2008. Chemical properates of pectin from
ambarella peels (Spondias cytherea) obtain using different
extraction condition. Food Chem 106: 1202-1207.
Kumar A, Chauhan, GS. 2010. Extraction and characterization of pectin
from apple pomace and its evaluation as lipase (steapsin) inhibitor.
Carbohydrate Pol 82: 454-459.
Madhav A, Pushpalatha PB. 2002. Characterization of pectin extracted
from different fruit wastes. J Trop Agric 40: 53-55.
Malviya R, Srivastava P, Bansal M, Sharma PK. 2010. Mango peel pectin
as super disintegrating agents. J Sci Industr Res 69: 688-690.
Mohamed HA. 1999. Extraction, Fraction and Characterization of Pectic
Substance of Grapefruit peels. [Thesis]. University of Khartoum,
Sudan.
Murkovic M, Mülleder U, Neunteu H. 2002. Carotenoid content in
different varieties of pumpkins. J Food Composit Anal 15: 633.
Ngouémazong DE, Tengweh FF, Fraeye I, Duvetter T, Cardinaels R, Loey
AV, Moldenaers P, Hendrickx M. 2012. Effect of demethylesterification network development and nature of Ca2+-pectin
gels: Towards understanding structureefunction relations of pectin.
Food Hydrocoll 26: 89-98.
Owens HS, McCready RM, Shepherd AD, Schultz TH, Pippen EL,
Swenson HA, Miers JC, Erlandsen RF, Macaly WD. 1952. Methods
Used at Western Regional Research-Laboratory for Extraction and
Analysis of Pectin Material. AIC 340. Albany, CA.
Ptichkina NM, Markina OA, Rumyantseva GN. 2008. Pectic extraction
from Pumpkin with the aid of microbial enzymes. J Food Hydrocoll
22: 192-195.
Ptichkina NM, Novokreschonova LV, Piskunova GV, Morris ER. 1998.
Large enhancements in loaf volume and organoleptic acceptability of
wheat bread by small additions of pumpkin powder: Possible role of
acetylated pectin in stabilizing gas-cell structure. J Food Hydrocoll
12: 333-337.
Ramli N, Asmawati. 2011. Effect of ammonium oxalate and acetic acid at
several extraction time and pH on some physicochemical properties
of pectin from cocoa husk (Theobroma cacao). African J Food Sci 5:
790-798.
Ranganna S. 1995. Hand Book of Analysis and Quality Control for Fruits
and Vegetable Products. 2nd ed. McGraw Hill Publishing Co. Ltd.,
New Delhi.
Sayah MJ, Chabir R, El Madani N, Rodi El Kandri Y, Ouazzani Chahdi F,
Touzani H, Errachidi F. 2014. Comparative study on pectin yield
according to the state of the orange peels and acids used. Intl J Innov
Res Sci Eng Technol 3 (8): 15658-15665.
Souza JRR, Carvalho JIX, Trevisan MTS, Paula RCM, Ricardo NMPS,
Feitosa JPA. 2009. Chitosan-coated pectin beads: characterization and
in vitro release of mangiferin. Food Hydrocoll 23 (8): 2278-2286.
Trease GE, Evans WC. 1989. Pharmacogsy. 13th ed. Bailliere Tindall,
London.
Vincken JP, Schols HA, Oomen RJFJ, Beldman G, Visser RGF, Voragen
AGJ. 2003. Pectin-the hairy thing. In: Voragen AGJ, Schols H, Visser
R (eds.). Advances in Pectin and Pectinase Research, Kluwer
Academic Publishers, Boston.
Wagner AE, Meeker WQ Jr. 1985. Proceding of the Statistical Computing
Section. Amer State Assoc., Washington, DC.
Waldron KW, Parker ML, Smith AC. 2003. Plant cell walls and food
quality. Comp Rev Food Sci Food Saf 2: 101-119.
Willats WGT, Knox JP, Mikkelsen DJ. 2006. Pectin: new insights into an
old polymer are starting to gel. Trends Food Sci Technol 17: 97.
Yeoh S, Shi J, Langrish T. 2008. A comparison between different
techniques for water based extraction of pectins from orange peels.
Chem Eng Commun 19: 511-520.