Abstract. Prihantini AI, Krisnawati, Setyayudi A. 2019. Antioxidant and alpha-glucosidase inhibitory activities of Euchresta horsfieldii. Biofarmasi J Nat Prod Biochem 17: 61-64. Euchresta horsfieldii, known as pranajiwa, is a medicinal plant that is widely grown in Bali and West Nusa Tenggara, Indonesia. Its seeds or fruits are commonly used for body freshness and stamina. The present study aimed to investigate the biological activities of leaves, root, stem, fruits, seeds of the E. horsfieldii. Antioxidant, alpha-glucosidase inhibitory activities and total phenolic compound were evaluated from methanolic extracts of all parts of E. horsfieldii. The result showed that leaf extract of E. horsfieldii exhibited the highest antioxidant activity with IC50 215.11±08.06 µg/mL. Meanwhile, the root extract had the highest alpha-glucosidase inhibitory activity and total phenolic compound with IC50 29.76±13.17 µg/mL and 763±0.01 mg GAE/100mg dry extract, respectively. In conclusion, the study suggested that E. horsfieldii is potential as natural source of antioxidant and alpha-glucosidase inhibitor agents.