Abstract. Authors. 2021. Antifungal activities of methanol extracts of some medicinal plants against germination and growth of Colletotrichum destructivum O’Gara in culture. Asian J Nat Prod Biochem 19: 25-29. Cowpea is an important grain and leafy vegetable in many tropical locations. Anthracnose caused by Colletotrichum destructivum O’Gara is one of the several factors constraining the economic production of the crop in warm humid areas. Use of resistant varieties and synthetic pesticides for control of the disease in the crop are disadvantaged for variability of the pathogen, leading to resistance failure of cultivars in many cowpea growing areas; and pesticide residues in the treated crops which ultimately engender mammalian toxicity respectively. This study evaluated the effects of Alchornea cordifolia, Tabernaemontana pachysiphon and Lantana camara as eco-friendly fungicides against the pathogen in culture. The results showed that the extracts inhibited the fungus in a dose-wise manner. At 75 and100 % concentrations, L. camara strongly inhibited the spore germination and radial growth of the fungus better than all concentrations of T. pachysiphon and A. cordifolia; whereas A. cordifolia extracts exhibited the lowest inhibition at all concentrations. Generally, the fungitoxicity of benomyl was found higher which inhibited 90.15 % spore germination and 96.32 % radial growth of the pathogen. However, at 75%, 100% L. camara and 100% T. pachysiphon which significantly (P?0.05) inhibited (80.02%, 84.21 % and 80.91%) spore germination and (87.33%, 90.87 % and 85. %) radial growth of the fungus respectively compared well with inhibition effects recorded from benomyl. Therefore these plants can be used as viable protectants of cowpea against anthracnose (C. destructivum) in small holder farming systems of the humid tropics; to enhance its production and farm economy.