Short Communication: Analysis of purity and concentration of extracted DNA on salted fish processed food products




Abstract. Sophian A. 2021. Short Communication: Analysis of purity and concentration of extracted DNA on salted fish processed food products. Asian J Nat Prod Biochem 19: 21-24. Analysis of purity and concentration of extracted DNA on salted fish processed food products was carried out in the microbiology and molecular biology testing laboratory of the Food and Drug Administration in Gorontalo. The purpose of this study was to analyze the extracted DNA on salted fish processed food products based on the concentration and purity values in the A260 / A230 and A260 / A280 wavelengths. The method used for purity and concentration analysis was the absorbance method using a nanophotometer. The samples used were 10 types of salted fish processed food products sampled from 5 (five) traditional markets in Gorontalo City. The sample was extracted using the spin column method with the Dneasy Mericon Food Kit (50) paint kit. 69514 (Qiagen). The research data showed that extracted sample concentration was in the range of 24,600 - 27,150 with an average of 25,745, while purity value measured at A260 / A280 wavelength was obtained with a purity range between 1,668 - 1,768 with an average of 1,729. Based on the results of this study, it can be concluded that the results of DNA extraction carried out on salted fish processed food products show a value that is in the category of good DNA extraction results.


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