Anti-inflammatory effects of Eucheuma denticulatum and Padina minor crude extracts in an egg albumin-induced paw edema of ICR mice

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Vol. 16 No. 2 (2024)
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Articles

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ALITA S. LABIAGA ♥
Department of Biology, College of Science, De La Salle University. 2401 Taft Avenue, 0922 Manila, Philippines
MICHAEL B. PLES
Department of Biology, College of Science, De La Salle University. 2401 Taft Avenue, 0922 Manila, Philippines
MARCO NEMESIO E. MONTAŇO
The Marine Sciences CS Institute, University of the Philippines. Velasquez Street University of the Philippines Diliman, Quezon City 1101, Philippines

Abstract

Abstract. Labiaga AS, Ples MB, Monta?o MNE. 2024. Anti-inflammatory effects of Eucheuma denticulatum and Padina minor crude extracts in an egg albumin-induced paw edema of ICR mice. Nusantara Bioscience 16: 245-250. Inflammation indicates that the body is unhealthy and exposed to harmful stimuli. Hence, it is important to study natural compounds with anti-inflammatory activities. This study assessed the anti-inflammatory effects of the aqueous and ethanolic crude extracts of Eucheuma denticulatum (N.L. Burman) F.S. Collins & Hervey and Padina minor (Yamada, 1925) in an egg albumin-induced paw edema of male Institute for Cancer Research (ICR) mice. This in vivo study investigated the anti-inflammatory activity of the aqueous and ethanolic crude extracts from E. denticulatum and P. minor at a dose of 100 mg/kg and compared it with a commercial anti-inflammatory drug (mefenamic). The study showed a significant decrease in the paw size, body temperature, neutrophil count, and white blood cell count in mice treated with seaweed crude extracts. The results of the first hour showed that the Eucheuma ethanolic extract treatment had the highest decrease in paw size, and the highest temperature reduction was found in the treatment of Padina ethanolic extract. There are significant differences in paw size, temperature, and leukocytes between the control and treatment groups, as well as between the aqueous and ethanolic extracts. The extracts from E. denticulatum significantly decrease the temperature, paw size, and leukocytes, which are the inflammation markers of egg albumin-induced paw edema. The diverse seaweed in the country has various pharmacological potential that should be studied further to maximize utilization.

2019-01-01

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References
Akinloye OA, Alagbe OA, Ugbaja RN, Omotainse SO. 2020. Evaluation of the modulatory effects of Piper guineense leaves and seeds on egg albumin-induced inflammation in experimental rat models. J Ethnopharmacol 255: 112762. DOI: 10.1016/j.jep.2020.112762.
Balasubramaniam V, Lee JC, Noh MFM, Ahmad S, Brownlee IA, Ismail A. 2016. Alpha-amylase, antioxidant, and anti-inflammatory activities of Eucheuma denticulatum (NL Burman) FS Collins and Hervey. J Appl Phycol 28: 1965-1974. DOI: 10.1007/s10811-015-0690-6.
Barbu E, Popescu MR, Popescu AC, Balanescu SM. 2022. Inflammation as a precursor of atherothrombosis, diabetes and early vascular aging. Intl J Mol Sci 23 (2): 963. DOI: 10.3390/ijms23020963.
Cotas J, Leandro A, Monteiro P, Pacheco D, Figueirinha A, Gonçalves AM, da Silva GJ, Pereira L. 2020. Seaweed phenolics: From extraction to applications. Mar Drugs 18 (8): 384. DOI: 10.3390/md18080384.
Dumilag RV, Crisostomo BA, Aguinaldo ZZA, Hinaloc LAR, Liao LM, Roa-Quiaoit HA, Dangan-Galon F, Zuccarello GC, Guillemin ML, Brodie J, Cottier-Cook EJ, Roleda MY. 2023. The diversity of eucheumatoid seaweed cultivars in the Philippines. Rev Fish Sci Aquac 31 (1): 47-65. DOI: 10.1080/23308249.2022.2060038.
Feng X, Wang X. 2018. Comparison of the efficacy and safety of non-steroidal anti-inflammatory drugs for patients with primary dysmenorrhea: a network meta-analysis. Mol Pain 14: 1744806918770320. DOI: 10.1177/1744806918770320.
Godlewska K, Michalak I, Tuhy ?, Chojnacka K. 2016. Plant growth biostimulants based on different methods of seaweed extraction with water. BioMed Res Intl 2016: 5973760. DOI: 10.1155/2016/5973760.
Guo Y, Flaherty MP, Wu WJ, Tan W, Zhu X, Li Q, Bolli R. 2012. Genetic background, gender, age, body temperature, and arterial blood pH have a major impact on myocardial infarct size in the mouse and need to be carefully measured and/or taken into account: Results of a comprehensive analysis of determinants of infarct size in 1,074 mice. Basic Res Cardiol 107: 288. DOI: 10.1007/s00395-012-0288-y.
Hurtado AQ, Magdugo R, Critchley AT. 2020. Harvesting and potential uses of selected red seaweeds in the Philippines with emerging high-value applications. Adv Bot Res 95: 19-56. DOI: 10.1016/bs.abr.2019.12.004.
Jayawardena TU, Sanjeewa KA, Nagahawatta DP, Lee HG, Lu YA, Vaas APJP, Abeytunga DTU, Nanayakkara CM, Lee DS, Jeon YJ. 2020. Anti-inflammatory effects of sulfated polysaccharide from Sargassum swartzii in macrophages via blocking TLR/NF-?b signal transduction. Mar Drugs 18 (12): 601. DOI: 10.3390/md18120601.
Khursheed M, Ghelani H, Jan RK, Adrian TE. 2023. Anti-inflammatory effects of bioactive compounds from seaweeds, bryozoans, jellyfish, shellfish and peanut worms. Mar Drugs 21 (10): 524. DOI: 10.3390/md21100524.
Labiaga AS, Monta?o MNE, Yba?ez AP, Ples MB. 2021. Phytochemical Profiling of Eucheuma denticulatum (NL Burman) Collins and Hervey, 1917 and Padina minor (Yamada, 1925) Seaweeds for Compounds of Potential Biomedical and Pharmaceutical Applications. DLSU Research Congress. De La Salle University, Manila, Philippines.
Lomartire S, Gonçalves AM. 2022. An overview of potential seaweed-derived bioactive compounds for pharmaceutical applications. Mar Drugs 20 (2): 141. DOI: 10.3390/md20020141.
Lomartire S, Marques JC, Gonçalves AM. 2021. An overview to the health benefits of seaweeds consumption. Mar Drugs 19 (6): 341. DOI: 10.3390/md19060341.
Magdugo R. 2020. Marine Seaweeds with Economic Importance in the Philippines: Valuation of Six Species Caulerpa racemosa (Forsskål), Ulva fasciata (Delile), Sargassum polycystum (C. Agardh), Sargassum ilicifolium (Turner) C. Agardh, Halymenia durvillei (Bory de Saint-Vincent), and Halymenia dilatata (Zanardini) from the Philippines. [Doctoral Dissertation]. Université de Bretagne Sud, Lorient, France.
Maleki SJ, Crespo JF, Cabanillas B. 2019. Anti-inflammatory effects of flavonoids. Food Chem 299: 125124. DOI: 10.1016/j.foodchem.2019.125124.
Marques CT, de Azevedo TC, Nascimento MS, Medeiros VP, Alves LG, Benevides N, Rocha HAO, Leite EL. 2012. Sulfated fucans extracted from the algae Padina gymnospora have anti-inflammatory effects. Rev Bras Farmacogn 22 (1): 115-122. DOI: 10.1590/S0102-695X2011005000206.
Masoumi S, Borugadda VB, Dalai AK. 2021. Techno-economic analysis of algal biorefineries. In: Dalai AK, Goud VV, Nanda S, Borugadda VB (eds). Algal Biorefinery: Developments, Challenges, and Opportunities. Routledge, London. DOI: 10.4324/9781003100317-11.
Pechroj S, Potiparsat K, Nangam O, Athipornchai A, Suriyaphan J, Muangham S, Srisook K. 2020. Comparative evaluation of antioxidant and anti-inflammatory activities of four seaweed species from the east coast of the Gulf of Thailand. Health Sci Technol Rev 13 (3): 11-21.
Peerapornpisal Y, Amornlerdpison D, Jamjai U, Taesotikul T, Pongpaibul Y, Nualchareon M, Kanjanapothi D. 2010. Antioxidant and anti-inflammatory activities of a brown marine alga, Padina minor Yamada. Chiang Mai J Sci 37(3): 507-516.
Ripol A, Cardoso C, Afonso C, Varela J, Quental-Ferreira H, Pousão-Ferreira P, Bandarra NM. 2018. Composition, anti-inflammatory activity, and bioaccessibility of green seaweeds from fish pond aquaculture. Nat Prod Commun 13 (5): 1934578X1801300521. DOI: 10.1177/1934578X1801300521.
Rivanor RLdC, Do Val DR, Ribeiro NA, Silveira FD, de Assis EL, Franco ÁX, Vieira LV, de Queiroz INL, Chaves HV, Bezerra MM, Benevides NMB. 2018. A lectin fraction from green seaweed Caulerpa cupressoides inhibits inflammatory nociception in the temporomandibular joint of rats dependent from peripheral mechanisms. Intl J Biol Macromol 115: 331-340. DOI: 10.1016/j.ijbiomac.2018.04.065.
Saraswati, Giriwono PE, Iskandriati D, Tan CP, Andarwulan N. 2019. Sargassum seaweed as a source of anti-inflammatory substances and the potential insight of the tropical species: A review. Mar Drugs 17 (10): 590. DOI: 10.3390/md17100590.
Tizard IR. 2017. Veterinary Immunology-E-Book: Veterinary Immunology-E-Book. Elsevier, Missouri.
Tumang VMC, Ples MB. 2017. Evaluating the anti-inflammatory activity of aqueous extract of Philippine Morinda citrifolia (Noni) juice on Albino rats (Rattus norvegicus) using carrageenan-induced paw edema. DLSU Research Congress. De La Salle University, Manila, Philippines.