Trimanto, Rahadiantoro A. 2017. Acclimatization of plant collection from Moyo Island Forest, West Nusa Tenggara, Indonesia at Purwodadi Botanic Garden. Trop Drylands 1: 43-49. A number of plant accessions were collected from Moyo Island forest, West Nusa Tenggara, Indonesia consisted of 123 accession numbers of general collection and 17 accession numbers of orchid collection. These plant materials were acclimatized at a nursery in Purwodadi Botanic Garden, Pasuruan, East Java, Indonesia. Acclimatization was aimed to determine successfully adapting plants from the forest, which are expected to survive and can be used as plants collection in Purwodadi Botanic Garden. The objective of this research was to identify the plant species of Moyo Island that can adapt in Purwodadi Botanic Garden and analyze the mechanism of their adaptation. The study was conducted at a nursery unit in 2013, with an observational method. The results show that 75% plant species collected from Moyo Island could adapt in Purwodadi Botanic Garden. We found 96 accessions of the general collection and nine accessions of the orchid collection that could adapt. The orchid collection was harder to adapt as compared to the general plant collection.