Activity test on laccase enzyme of fungus Volvariella volvacea for decolorization of remazol reactive dye

Abstract

Abstract. Sari TP, Susilowati A, Setyaningsih R. 2022. Activity test on laccase enzyme of fungus Volvariella volvacea for decolorization of remazol reactive dye. Asian J Trop Biotechnol 18: 73-78. Textile industry waste such as remazol reactive dye is a source of pollution that can damage aquatic ecosystems. This waste is difficult to degrade because it has strong covalent bonds. However, this compound can be degraded biologically (decolorization) using the laccase enzyme oxidoreductase. This study aimed to determine the ability of the laccase enzyme produced by the fungus Volvariella volvacea (Bulliard ex Fries) Singer to decolorize remazol reactive dye. The test begins with the decolorization of the dye by the fungal culture of V. volvacea, the production and purification of the laccase enzyme with ammonium sulfate, and the decolorization test with the purified laccase enzyme. The results showed that the fungal culture of V. volvacea was able to decolorize remazol reactive dye on agar and liquid medium enzymatically. In addition, based on a statistical analysis of ANOVA followed by a Post-Hoc Test DMRT method test level of 5%, the results showed that the percentage of decolorization of the three dyes by the laccase enzyme, which had the highest enzyme activity (5.42 U/mL) from the ammonium sulfate purification increased along with increasing incubation time. The highest decolorization percentage occurred in remazol brilliant blue R (RBBR) with an incubation time of 48 hours.