Isolation and characterization of lactic acid bacteria from the gut of the grasscutter (Thryonomys swinderianus)
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Abstract. Agbove MM, Kayang BB, Futse JE. 2021. Isolation and characterization of lactic acid bacteria from the gut of the grasscutter (Thryonomys swinderianus). Asian J Trop Biotechnol 18: 79-85. Intestinal microbiota can affect hosts either beneficially or harmfully. Many efforts have been made to identify and study the microbial community in the gastrointestinal tract of livestock. The grasscutter is a micro-livestock species whose intestinal microflora is yet to be explored. Lactic acid bacteria confer probiotic benefits among the intestinal microflora and are of special interest. This study was conducted to isolate and characterize lactic acid bacteria from the gut of grasscutters in Ghana. Fresh fecal samples were collected from a total of 26 grasscutters comprising nine domesticated grasscutters and 17 wild grasscutters from Ghana. The samples were cultured on MRS agar, and the DNA from 57 bacterial colonies was extracted and sequenced at the 16S rRNA gene to identify the bacteria at the species level using the Basic Local Alignment Search Tool in the National Centre for Biotechnology Information database. Some of the five genera comprising 15 species of lactic acid bacteria (LAB) were identified with ?99% similarity. Those included Lactobacillus fermentum (n = 11), L. formosensis (n = 1), L. salivarius (n = 11), L. ingluviei (n = 9), L. plantarum (n= 7), L. reuteri (n = 2), L. taiwanensis (n = 1), L. rhamnosus (n= 1), Pediococcus pentosaceus (n = 5), Enterococcus gallinarum (n = 2), E. faecium (n = 2), Staphylococcus homini (n = 2), Weissella cibaria (n = 1), E. hirae (n = 2), and W. paramesenteroides (n = 1). Moreover, all five genera were isolated from the domesticated grasscutters, while only two genera (Lactobacillus and Pediococcus) were isolated from wild grasscutters. The isolation of L. ingluviei is very interesting since this species was originally isolated from birds and is associated with weight gain in mice. The bacteria identified in this study may be important in determining the intestinal health of the grasscutter and should be assessed for their potential as probiotics to improve grasscutter nutrition.
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