Short Communication: Identification of spoilage fungi in Myristica fragrans using DG18 and CYA Media

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Vol. 18 No. 2 (2021)
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Articles

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MIFTAHUL HUDA FENDIYANTO ♥
Department of Biology, Faculty of Military Mathematics and Natural Sciences, Universitas Pertahanan Indonesia. Kampus Universitas Pertahanan Kawasan IPSC, Sentul, Bogor 16810, West Java, Indonesia
https://orcid.org/0000-0002-0502-2669
RIZKY DWI SATRIO
Department of Biology, Faculty of Military Mathematics and Natural Sciences, Universitas Pertahanan Indonesia. Kampus Universitas Pertahanan Kawasan IPSC, Sentul, Bogor 16810, West Java, Indonesia
https://orcid.org/0000-0003-1716-2076
MENTARI PUTRI PRATAMI
Department of Biology, Faculty of Military Mathematics and Natural Sciences, Universitas Pertahanan Indonesia. Kampus Universitas Pertahanan Kawasan IPSC, Sentul, Bogor 16810, West Java, Indonesia
ISNA AROFATUN NIKMAH
Department of Biology, Faculty of Military Mathematics and Natural Sciences, Universitas Pertahanan Indonesia. Kampus Universitas Pertahanan Kawasan IPSC, Sentul, Bogor 16810, West Java, Indonesia

Abstract

Abstract. Fendiyanto MH, Satrio RD, Pratami MP, Nikmah IA. 2021. Short Communication: Identification of spoilage fungi in Myristica fragrans using DG18 and CYA Media. Asian J Trop Biotechnol 18: 51-54. To date, very few studies have been conducted on the identification of spoilage fungi in Myristica fragrans. Therefore, this study aimed to identify the spoilage fungi in Myristica fragrans growing on DG18 media and CYA Identification Media. Isolation of the fungus was carried out by the dilution plating method using a dilution range of 10-1 to 10-5. The isolation results at 10-2 and 10-3 dilutions on nutmeg (Myristica fragrans) seed samples indicated the presence of Aspergillus niger and A. flavus. The fungal species that could be found and identified using the direct plating method were A. niger, A. flavus, Penicillium citrinum, and Fusarium sp. The results of fungal isolation showed that the species of spoilage fungi on postharvest of M. fragrans seeds, including Aspergillus flavus, A. niger, A. tamarii, Endomyces fibuliger, Eurotium chevalieri, E. repens, and Penicillium citrinum, Trichoderma sp. The highest population of spoilage fungi isolated from Myristica fragrans seeds was Aspergillus niger. This finding indicates that A.niger can be identified as spoilage fungi isolated from Myristica fragrans.

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