Short Communication: Comparison of two commercial DNA extraction kit to obtain high quality porcine DNA

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Vol. 18 No. 2 (2021)
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Articles

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DIANA TRI WULAN ♥
Special Drug and Food Testing Center, National Center for Drug and Food Testing Development, National Agency of Drug and Food Control Indonesia. Jl. Percetakan Negara No. 23, Jakarta Pusat 10560, Jakarta, Indonesia
MARCELLA SUTANTA
Special Drug and Food Testing Center, National Center for Drug and Food Testing Development, National Agency of Drug and Food Control Indonesia. Jl. Percetakan Negara No. 23, Jakarta Pusat 10560, Jakarta, Indonesia
ALFI SOPHIAN
Special Drug and Food Testing Center, National Center for Drug and Food Testing Development, National Agency of Drug and Food Control Indonesia. Jl. Percetakan Negara No. 23, Jakarta Pusat 10560, Jakarta, Indonesia

Abstract

Abstract. Wulan DT, Sutanta M, Sophian A. 2021. Short Communication: Comparison of two commercial DNA extraction kit to obtain high quality porcine DNA. Asian J Trop Biotechnol 18: 69-72. Comparison of two commercial DNA extraction kits, to obtain high-quality porcine DNA, was carried out to give information about the capabilities of the two types of commercial kits used in isolating DNA. This research was conducted to provide information on the quality of DNA isolated from the manufacture of standard DNA from two different types of extraction kits. The kits used in this study were the Mericon Food Kit and the Life River Kit. The method used in this study was the centrifuge column extraction method. The isolated sample was analyzed for purity and concentration using a nano spectrophotometer. The data obtained was then processed statistically to obtain P-value for two mean T-test. Based on the study results, it was found that the average purity and concentration of DNA extracted with the Mericon Food Kit were 2.17 ng/µL and 139.3 ng/µL. Meanwhile, the average purity and concentration of DNA isolated from the Life River Kit were 1.46 ng/µL and 11.9 ng/µL. Based on the statistical analysis of the P-value for the two mean T-test, it was found that the purity and concentration of the two extraction kits were significantly different.

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