Quality diversity of 35 tea clones (Camellia sinensis var. sinensis) processed for green tea

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M. KHAIS PRAYOGA
HERI SYAHRIAN
VITRIA P. RAHADI
M. IQBAL PRAWIRA ATMAJA
HILMAN MAULANA
ANAS

Abstract

Abstract. Prayoga MK, Syahrian H, Rahadi VP, Atmaja MIP, Maulana H, Anas. 2022. Quality diversity of 35 tea clones (Camellia sinensis var. sinensis) processed for green tea. Biodiversitas 23: 810-816. The development of sinensis clones in Indonesia is still focused on increasing productivity and until now there has been no study on the level of genetic similarity and diversity based on quality characters. Therefore, the purpose of this study was to examine the level of kinship and genetic diversity of 35 sinensis clones from the collection of the Indonesia Research Institute for Tea and Cinchona. The study consisted of two stages, testing the content of chemical compounds (polyphenols and caffeine) and organoleptic testing. The material used in both tests was fresh shoots consisting of peko + 3 young leaves (P+3). In organoleptic testing, shoots of 35 sinensis variety clones were first processed into green tea. Furthermore, the processed green tea was tested organoleptically by three credible panelists. The data from the test results of chemical and organoleptic compounds were then tested statistically to get the coefficient of variation (CV), which indicates data diversity. The level of genetic closeness between clones based on quality characters was analyzed using the JMP 16.0 program (trial version), then arranged into clusters. To see the contribution of each parameter to the quality diversity of 35 sinensis clones, a principal component analysis (PCA) was carried out. The results of this study indicate that there are four main clusters grouping the 35 sinensis clones tested. The first cluster excels in the character of taste and aroma, the second cluster excels in appearance, seductive colour, and infusion, the third cluster excels in the character of polyphenols, while the fourth cluster excels in the character of caffeine. The characters that contributed to the genetic diversity of the 35 clones tested were caffeine, appearance, and aroma.

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References
Acquaah, G. 2007. Principles of Plant Genetics and Breeding. Blackwell Publishing, New Jersey.
Al-Naggar AM, Shafik MM, Musa RYM. 2020. Genetic Diversity Based on Morphological Traits of 19 Maize Genotypes Using Principal Component Analysis and GT Biplot. Annual Research and Review in Biology 35 : 68-85. DOI: 10.9734/ARRB/2020/v35i230191.
Anggraini T. 2017. Tea Process and Benefits. Rumah Kayu Pustaka Utama, Padang.
Anon. 2005. ISO 14502–1:2005 (E) Determination of substances characteristic of green and black tea- Part 1: Content of total polyphenols in a tea-colorimetric method using Folin-Ciocalteu reagent. Int. Stand. ISO.
Bao YX, Zhang J, Tang YC, Liu DH, Yang MXR, Huang. 2008. Breeding of a new hybrid green tea cultivar “foxing 3” with fine quality. Guizhou Science 26 : 66–70.
Cakra GA, Munandar JM. 2020. Competitiveness Analysis of Indonesian Bulk Black Tea Commodity in Global Market (Case Study in Russia). Journal of Management and Organization 11 : 57-70.
Campbell MJ, Machin, D, Walters S J. 2010. Medical statistics: a textbook for the health sciences. John Wiley & Sons, New Jersey.
Charlesworth D, Willis JH. 2009. The genetics of inbreeding depression. Nature Reviews Genetics 10 : 783–796.
Diksha K, Rohit C, Anshul. 2020. A Review Article On Antioxidant Profile Of Green Tea Polyphenols In The Treatment Of Various Diseases. World Journal Of Pharmacy And Pharmaceutical Sciences 9 : 1646-1663. DOI: 10.20959/wjpps20206-16378.
Gour L, Maurya SB, Koutu GK, Singh SK, Shukla S S, Mishra DK. 2017. Characterization of rice (Oryza sativa L.) genotypes using principal component analysis including scree plot & rotated component matrix. International Journal of Chemical Studies 5 : 975-983.
Harborne J. 1996. Phytochemical Methods: A Guide to Modern Ways of Analyzing Plants. Second printing. Translators: Padmawinata K, Soediro I. Institut Teknologi Bandung, Bandung.
Hostettmann K, Hostettmann M, Marston A. 1985. Method of Preparative Chromatography: Use in Isolation of Natural Compounds. Translator: Padmawinata K. Institut Teknologi Bandung, Bandung.
Martono B, Syafaruddin. 2018. Genetic variability of 21 tea genotypes (Camellia Sinensis (l.) O. Kuntze) based on rapd markers. Journal of Industrial and Beverage Crops 5 : 77-86.
Martono B, Udarno L. 2015. Caffeine Content And Characteristics Of Shoot Morphology Of Six Tea Genotypes. Journal of Industrial and Beverage Crops 2 : 69-76.
National Standardization Agency. (2016). National Standard Indonesia 3945. National Standards Agency, Jakarta.
Owuol PO, Obaga SO, Othieno CO. 1990. The effects of altitude on the chemical composition of black tea. J. Sci. Food Agric 50 : 9-17.
Owuor PO, Obanda M, Nyirenda HE, Mphangwe NIK, Wright LP, Apostolides Z. 2006. The relationship between some chemical parameters and sensory evaluations for plain black tea (Camellia sinensis) produced in Kenya and comparison with similar teas from Malawi and South Africa. Food Chemistry 97 : 644–653. DOI: 10.1016/j.foodchem.2005.04.027.
Prawira-Atmaja MI, Maulana H, Shabri, Riski GP, Fauziah, A, Harianto, S. 2021. Evaluation of the conformity of the quality of tea products with the requirements of the Indonesian National Standard. Standardization Journal 23 : 43-52.
Prawira-Atmaja, MI, Shabri, Khomaini HS, Maulana H, Harianto S, Rohdiana D. 2010. Changes in chlorophyll and polyphenols content in Camellia sinensis var. Sinensis at a different stage of leaf maturity. IOP Conference Series: Earth and Environmental Science, Volume 131, International Conference on Green Agro-industry and Bioeconomy (ICGAB 2017) 24–25 October 2017, Batu City, East Java, Indonesia.
Rahadi VP, Khomaeni HS, Chaidir L. Martono B. 2016. Genetic Diversity And Relationships Of Tea Germplasm Collection Based On Leaf Morphology Character And Yield Components. Journal of Industrial and Beverage Crops 3 : 103–108.
Rohdiana D. 2009. Polyphenol Radical Increasing Activity in Tea Leaves. Alfabeta, Bandung.
Rohlf FJ. 2001. NTSYSpc Numerical Taxonomy and Multivariate Analysis System. Exeter Software, New York.
Sahar R, Rahimmalek M, Goli SAH. 2016. Evaluation of seven different drying treatments in respect to total flavonoid, phenolic, vitamin C content, chlorophyll, antioxidant activity and color of green tea (Camellia sinensis or C. Assamica) leaves. J Food Sci Technol 53 : 721–729. Doi: 10.1007/s13197-015-2030-x.
Shabri dan Rohdiana, D. 2016. Optimization and characterization of green tea polyphenol extracts from various solvents. Tea and Cinchona Research Journal 19 : 57-66.
Simarmata T, Prayoga, MK, Setiawati, MR, Adinata K, Stöber S. 2021. Improving the Climate Resilience of Rice Farming in Flood-Prone Areas through Azolla Biofertilizer and Saline-Tolerant Varieties. Sustainability 13: 1-9. DOI: 10.3390/su13211230.
Sita K, Rohdiana D. 2021. Tea Commodity Performance and Prospect Analysis. Radar De Plantation: Plantation Analysis and Opinion 2 : 1-12.
Sriyadi B. 2011. Superior Sinensis tea clones release of GMBS 1, GMBS 2, GMBS 3, GMBS 4, and GMBS 5. Tea and Cinchona Research Journal 14 : 59-71.
Temple SJ, Temple CM, van Boxtel T. 2001. The effect of drying on black tea quality. Journal of the Science of Food and Agriculture 81 : 764 – 772. Doi: 10.1002/jsfa.881.
Towaha J. 2013. The content of chemical compounds in tea leaves (Camellia sinensis). Industrial Crops Research and Development News 19 : 12-16.
Yamanishi T. 1978. Flavor of Green Tea. Japan Agricultural Research Quarterly 12 : 205-210.