Estimation of genetic parameters and heterosis through line × tester crosses of national sorghum varieties and local Indonesian cultivars




Abstract. Rachman F, Trikoesoemaningtyas, Wirnas D, Reflinur. 2022. Estimation of genetic parameters and heterosis through line × tester crosses of national sorghum varieties and local Indonesian cultivars. Biodiversitas 23: 1588-1597. Diverse gene sources possessed by local sorghum cultivars are potentially useful for superior varieties development. This study aimed to determine the genetic parameters and heterosis of sorghum lines derived from national varieties and local cultivars crosses. A total of eight F1 hybrids derived from line × tester mating design with four lines (PI-150-20A, Soraya 3 IPB, Kawali, Bioguma 1 Agritan) and two testers (Pulut 3 and Pulut 5) were used in the present study. This experiment was arranged in a randomized complete block design (RCBD) with three replications. Results showed that several agronomical important traits, such as plant height, leaf number, days to flowering, days to harvesting, panicle length, panicle diameter, and 1000-grain weight were significantly affected by additive gene action, while others by non-additive gene action. Based on field observation, three out of six parental lines showed the best performance of grain yields traits. Of these, Soraya 3 IPB × Pulut 5 and Bioguma 1 Agritan × Pulut 5 crosses were the best cross combination showing high values for both combining ability and heterosis parameters. These findings would help breeders to determine the selection methods for desirable traits and cross combinations to develop a new variety with high yield performance.


Abebe A, Wolde L, Gebreselassie W. 2020. Standard heterosis and trait association of maize inbred lines using line x tester mating design in Ethiopia. Afric J Plant Sci. 14(4):192-204. DOI: 10.5897/AJPS2019.1839.
Abubakar L, Bubuche TS. 2013. Correlation analysis of some agronomic traits for biomass improvement in sorghum (Sorghum Bicolor L. Moench) genotypes in north-western Nigeria. Afr J Agric Res 8(28): 3750-3756. DOI: 10.5897/AJAR2013.6858.
Acquaah G. 2007. Principles of Plant Genetics and Breeding. Blackwell Publishing, Oxford.
Agung IGAMS, Sardiana IK, Diara IW, Nurjaya IGMO. 2014. Residual effect of compost on ethanol production of sweet sorghum [Sorghum bicolor (L.) Moench] varieties and soil organic carbon at dryland farming area in Bali, Indonesia. J Biol Agric Healthcare 4(13): 96-102.
Akpertey A, Anim-Kwapong E, Adu-gyamfi PKK, Ofori A. 2020. Genetic variation among biparental Robusta coffee families and implications for variety development. Experimental Agric 56(2): 171-182. DOI: 10.1017/S0014479719000255.
Amorim AFS, Gilio TAS, de Jesus JB, de Souza LHA, dos Anjos IV, Araujo KL, Barelli MAA, Preisigke SC, Neves LG. 2021. Genetic improvement of Capsicum frustescens: hybrid vigor for anthracnosis resistance and production traits. 217:72. DOI: 10.1007/s10681-021-02810-2.
Andriani A, Isnaini M. 2013. Morfologi dan fase pertumbuhan sorgum. In Sumarno, Damardjati DS, Syam M, Hermanto (eds) Sorgum: Inovasi Teknologi dan Pengembangan. IAARD Press, Jakarta. [Indonesian]
Benggu YI, Nguru ESO. 2018. Short communication: the tolerance level of local sorghum genotypes from Sabu-Raijua and Belu Districts, Indonesia to saline soil. Trop Drylands 2(1): 1-4. DOI: 10.13057/tropdrylands/t020101.
Daryanto A, Syukur M, Maharijaya A, Hidayat P. 2018. Diallel analysis of chili pepper resistance to melon aphid (Aphis gossypii Glover) infestation in seedling phase. Agrotech J 3(2): 61-68. DOI: 10.31327/atj.v3i2.868.
de Mendiburu F, Simon R. 2015. Agricolae-Ten years of an open source statistical tool for experiments in breeding, agriculture and biology. PeerJ 3: e1404v1. DOI: 10.7287/peerj.preprints.1404v1.
de Mendiburu F. 2021. Agricolae: Statistical Procedures For Agricultural Research.
Enyew M, Feyissa T, Geleta M, Tesfaye K, Hammenhag C, Carlsson AS. 2021. Genotype by environment interaction, correlation, AMMI, GGE biplot and cluster analysis for grain yield and other agronomic traits in sorghum (Sorghum bicolor L. Moench). PLoS ONE 16(10): e0258211. DOI: 10.1371/journal.pone.0258211.
Etuk E, Ifeduba AV, Okata UE, Chiaka I, Charles OI, Okeudo NJ, Esonu BO, Udedibie ABI, Moreki J. 2012. Nutrient composition and feeding value of sorghum for livestock and poultry. J Anim Sci Adv 2: 510-524.
FAO. 1995. Sorghum and millets in human nutrition. FAO.
Fitrahtunnisa, Mardian I, Rahmatullaila. 2019. Performance and utilization of local sorghum (Sorghum bicolor L.) in West Nusa Tenggara. IOP Conf Ser: Earth Environ Sci 484: 012092. DOI: 10.1088/1755-1315/484/1/012092.
Gomes GP, Zeffa DM, Constantino LV, Baba VY, Silvar C, Pomar F, Rodrigues R, Gonçalves LSA. 2021. Diallel analysis of the morphoagronomic, phytochemical, and antioxidant traits in Capsicum baccatum var. pendulum. Hortic Environ Biotechnol 62: 435-446. DOI: 10.1007/s13580-020-00299-7.
Goulet BE, Roda F, Hopkins R. 2017. Hybridization in plants: old ideas, new techniques. Plant Physiol. 173(1): 65-78. DOI: 10.1104/pp.16.01340.
Hidayanti N. 2020. Characterization of local varieties, national varieties and breeding lines of sorghum. [Thesis]. IPB University, Bogor. [Indonesian]
Huang M. Chen L, Chen Z. 2015. Diallel analysis of combining ability and heterosis for yield and yield components in rice by using positive loci. Euphytica 205: 37-50. DOI: 10.1007/s10681-015-1381-8.
Ingle KP, Gahukar SJ, Khelurkar VC, Ghorade RB, Kalpande VV, Jadhav PV, Moharil MP. 2018. Heterosis and combining ability for grain yield trait in Rabi sorghum [Sorghum bicolor (L.) Moench] using line x tester mating design. Int J Curr Microbiol App Sci 6: 1925-1934.
Janaki M, Babu JD, Naidu LN, Ramana CV, Rao KK, Krishna KU. 2018. Estimation of heterosis for earliness, yield and yield attributing traits in chilli (Capsicum annuum L.). Electronic J Plant Breed. 9(2): 543-550. DOI: 10.5958/0975-928X.2018.00066.2.
Jarwar AH, Wang X, Wang L, Ma Q, Fan S. 2017. Line x tester analysis of estimating heterosis and combining ability in F1 generation of sunflower. Asian Agric Res. 9(8): 70-74. DOI: 10.19601/j.cnki.issn1943-9903.2017.08.017.
Kamara MM, Ghazy NA, Mansour E, Elsharkawy MM, Kheir AMS, Ibrahim KM. 2021. Molecular genetic diversity and line × tester analysis for resistance to late wilt disease and grain yield in maize. Agronomy 11: 898. DOI: 10.3390/agronomy11050898
Kempthorne O. 1957. An Introduction to Genetic Statistics. John Wiley and Sons, New York.
Kenga R, Alibi SO, Gupta SC. 2003. Combining ability studies in tropical sorghum (Sorghum bicolor (L.) Moench). Field Crop Res 88: 251-260.
Khadi PS, Biradar BD, Pattanashetti SK. 2018. Heterosis studies for yield and yield components in rabi sorghum [Sorghum bicolor (L.) Moench]. J Farm Sci 31(3): 342-343.
Kose A. 2017. Gene action and combining abitiy in line X tester population of safflower (Carthamus tinctorius L.). Turkish J Field Crop 22(2): 197-203. doi:10.17557/tjfc.356216.
Krystkowiak K, Adamski T, Surma M, Kaczmarek Z. 2009. Relationship between phenotypic and genetic diversity of parental genotypes and the specific combining ability and heterosis effects in wheat (Triticum aestivum L.). Euphytica 165: 419-434. DOI: 10.1007/s10681-008-9761-y.
Kumar A, Mishra VK, Vyas RP, Singh V. 2011. Heterosis and combining ability analysis in bread wheat (Triticum aestivum L.). J Plant Breed Crop Sci 3(10): 209-217. DOI: 10.5897/JPBCS.9000075
Lestari T, Sopandie D, Trikoesoemaningtyas, Ardie SW. 2014. Screening of several sorghum genotypes on acid soil tolerance. IJAAR 5(5): 170-176.
Maftuchah, Febriana L, Sulistyawati, Reswari HA, Septia ED. 2021. Morphological diversity and heritability of nine local sorghum (Sorghum bicolor) genotypes in East Java, Indonesia. Biodiversitas 22(3): 1310-1316. DOI: 10.13057/biodiv/d220330.
Makanda I, Tongoona P, Derera J. 2009. Combining ability and heterosis of sorghum germplasm for stem sugar traits under off-season conditions in tropical lowland environments. Field Crop Res 114: 272-279. DOI: 10.1016/j.fcr.2009.08.009.
Makanda I. 2017. Development of dual purpose sorghum: correlation and path-coefficient analysis of grain yield and stem sugar traits. Afr Crop Sci J 25(3): 263-275. DOI: 10.4314/acsj.v25i3.1.
Martiwi INA, Nugroho LH, Daryono BS, Susandarini R. 2020. Morphological variability and taxonomic relationship of Sorghum bicolor (L.) Moench accessions based on qualitative characters. Annu Res Rev Biol 35(6): 40-52. DOI: 10.9734/arrb/2020/v35i630234.
Maurya KN, Pal PK, Asthana G, Srivastava A, Shukla S. 2017. Assessment of various genetic components through NCD-I and NCD-III design of biparental mating in opium poppy. J Genet 98: 27. DOI: 10.1007/s12041-019-1074-5.
Mengistu G, Shimelis H, Laing M, Lule D, Mashilo J. 2020. Combining ability and heterosis among sorghum (Sorghum bicolor [L.] Moench) lines for yield, yield-related traits, and anthracnose resistance in western Ethiopia. Euphytica 216: 33. DOI: 10.1007/s10681-020-2563-6.
Mohammed R, Are AK, Bhavanasi R, Munghate RS, Kavi Kishor PB, Sharma HC. 2015. Quantitative genetic analysis of agronomic and morphological traits in sorghum, Sorghum bicolor. Front Plant Sci 6: 945. DOI: 10.3389/fpls.2015.00945.
Mondal C, Sarkar S, Hazra P. 2009. Line × tester analysis of combining ability in tomato (Lycopersicon esculentum Mill.). J Crop and Weed 5(1): 53-57.
Mukkun L, Lalel HJD, Kleden YL. 2021. The physical and chemical characteristics of several accessions of sorghum cultivated on drylands in East Nusa Tenggara, Indonesia. Biodiversitas 22(5): 2520-2531. DOI: 10.13057/biodiv/d220509.
Mukkun L, Lalel HJD, Richana N, Pabendon MB, Kleden SR. 2018. The diversity of local sorghum (Sorghum bicolor L. Moench) in Nusa Tenggara Timur province. IOP Conf Ser: Earth Environ Sci 144: 012065. DOI: 10.1088/1755-1315/144/1/012065.
Muturi PW, Mgonja M, Rubaihayo P. 2019. Gene action conditioning resistance traits to spotted stem borer, Chilo partellus, in grain sorghum. Int J Trop Insect Sci 39: 147-155. DOI: 10.1007/s42690-019-00020-x.
Narasimhamurthy YK, Gowda PHR. 2013. Line × tester analysis in tomato (Solanum lycopersicum L.): identification of superior parents for fruit quality and yield-attributing traits. Int J Plant Breed 7(1): 50-54.
Nduwumuremyi A, Tongoona P, Habimana S, Husbandry A. 2013. Mating designs: helpful tool for quantitative plant breeding analysis. J Plant Breed Genet. 01(03): 117-129.
Ni X, Zhao G, Liu T, Hu J, Chen G, Ding G. 2012. Analysis on the combining ability and heritability of main agronomic traits of hybrid glutinous sorghum. J Agric Sci Technol 13(10): 2104-2109.
Panwar LL. 2005. Line × tester analysis of combining ability in rice (Oryza sativa L.). Indian J Genet 65(1): 51-52.
Prabhakar, Elangovan M, Bahadure DM. 2013. Combining ability of new parental lines for flowering, maturity and grain yield in Rabi sorghum. Electron J Plant Breed 4(3): 1214-1218.
Rehana S, Ullah MZ, Zeba N, Narzis, Husna A, Siddique AB. 2019. Estimation of heterosis for yield and yield attributing traits in tomato crossed with line and tester method. Progress Agric. 30(2): 179-185.
Rini EP, Wirnas D, Trikoesoemaningtyas, Sopandie D. 2017. Genetic analysis on agronomic and quality traits of sorghum hybrids in Indonesia. SABRAO J Breed Genet 49(2): 192-200.
Rivera-Burgos LA, Volenec JJ, Ejeta G. 2019. Biomass and bioenergy potential of brown midrib sweet sorghum germplasm. Front Plant Sci 10: 1142. DOI: 10.3389/fpls.2019.01142.
Rodríguez F, Alvarado G, Pacheco Á, Crossa J, Burgueño J. 2015. AGD-R (Analysis of Genetic Designs with R for Windows) Version 4.0. .
SaadAbdel-Aty M, Yousef SA, Ghazy MM, Hatab SH. 2018. Combining ability of forage yield for sorghum-sudangrass hybrids under water stress using line × tester. Fresenius Environ Bull 27(12): 8367-8379.
Sanghera GS, Hussain W. 2012. Heterosis and combining ability estimates using line x tester analysis to develop rice hybrids for temperate conditions. Not Sci Biol 4(3): 131-142. DOI: 10.15835/nsb437873.
Sapkal V, Patil S, Sapkal D, Yadav R, Ramawat N. 2018. Assessment of genetic variation and parameters among advanced biparental progeny lines (BIP F3) Brasscia juncea. Plant Archives 18(2): 1689-1694.
Schaffasz A, Windpassinger S, Snowdon R, Wittkop B. 2019. Reproductive cold stress tolerance in sorghum F1 hybrids is a heterotic trait. Agronomy 9: 508. DOI: 10.3390/agronomy9090508.
Sen R, Saini RK, Singh SK, Kumar A. 2019. Study of genetic variability of fodder yield and it’s components in forage sorghum [Sorghum bicolor (L.) Moench]. Forage Res 45(2): 156-158.
Sesay S, Ojo D, Ariyo OJ, Meseka S. 2016. Genetic variability, heritability and genetic advance studies in top-cross and three-way cross maize (Zea mays L) hybrids. Maydica 61(2): M12.
Singh NK, Singh AK, Singh AK, Misra VM, Mall AK. 2019. Estimates of genetic parameters, components of variance and their magnitude in rice (Oryza sativa L.). Plant Archives 19: 1105-1107.
Singh RK, Chaudary BD. 1979. Biometrical Methods in Quantitative Genetic Analysis. Kalyani Publishers, Ludhiana.
Sirappa MP. 2003. Prospek pengembangan sorgum di Indonesia sebagai komoditas alternatif untuk pangan, pakan, dan industri. Jurnal Litbang Pertanian 22(4): 133-140. [Indonesian]
Sofiah II. 2021. Performance of characters from local varieties, national varieties, and breeding lines of sorghum. [Thesis]. IPB University, Bogor. [Indonesian]
Subagio H, Aqil M. 2013. Development of sorghum production in Indonesia. Proceeding of National Seminar on Agriculture Innovation. p. 199-214.
Suguna M, Aruna C, Deepika C, Ratnavathi CV, Tonapi VA. 2021. Genetic analysis of semolina recovery and associated traits- a step towards breeding for specific end uses in sorghum (Sorghum bicolor (L.) Moench. J Cereal Sci 100: 103226. DOI: 10.1016/j.jcs.2021.103226.
Sulistyawati, Roeswitawati D, Ibrahim JT, Maftuchah. 2019. Genetic diversity of local sorghum (Sorghum bicolor) genotypes of East Java, Indonesia for agro-morphological and physiological traits. Biodiversitas 20(9): 2503-2510. DOI: 10.13057/biodiv/d200910.
Syukur M, Sujiprihati S, Yunianti R. 2012. Teknik Pemuliaan Tanaman. Penebar Swadaya, Jakarta. [Indonesian]
Tabri F, Zubachtirodin. 2013. Budi daya tanaman sorgum. In Sumarno, Damardjati DS, Syam M, Hermano (eds). Sorgum: Inovasi Teknologi dan Pengembangan. IAARD Press, Jakarta. [Indonesian]
Tnunay IMY, Chikmawati T, Miftahudin. 2019. Morphological diversity of local sorghum cultivar (Sorghum bicolor) of East Nusa Tenggara, Indonesia. Biosaintifika 11(1): 47-54.
Wagaw K, Tadesse T. 2020. Combining ability and heterosis of sorghum (Sorghum bicolor L. Moench) hybrids for grain and biomass yield. Am J Plant Sci 11: 2155-2171. DOI: 10.4236/ajps.2020.1112151.
Wickham H. 2016. ggplot2: Elegant Graphics for Data Analysis. Springer-Verlag, New York.
Widowati S. 2010. Karakteristik mutu gizi dan diversifikasi pangan berbasis sorgum (Sorghum vulgare). Jurnal Pangan 19(4): 373-382. DOI: 10.33964/jp.v19i4.170. [Indonesian]
Yehia H, El-Hashash EF. 2019. Combining ability effects and heterosis estimates through line x tester analysis for yield, yield components and fiber traits in Egyptian cotton. J Agron Technol Eng Manag. 2(2):248–262
Zhu Y, Wu S, Xu J, Lu Z, Li G, Hu Y, Yang X, Bush D. 2017. Genetic parameters for growth traits and stem-straightness in Eucalyptus urophylla × E. camaldulensis hybrids from a reciprocal mating design. Euphytica 213: 142. DOI: 10.1007/s10681-017-1923-3.

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