The potential of Lactobacillus buchneri isolated from spontaneous rabbit meat fermented - bekasam against Salmonella typhimurium by in vivo evaluation




Abstract. Wulandari E, Yurmiati H, Subroto T, Wismandanu O, Khairani S. 2022. The potential of Lactobacillus buchneri isolated from spontaneous rabbit meat fermented - bekasam against Salmonella typhimurium by in vivo evaluation. Biodiversitas 23: 2304-2310. Rabbit meat bekasam is a traditional fermented food and is considered a healthy and functional food due to its nutrient content and microorganism. Lactic acid bacteria (LAB) was dominant microorganism and Lactobacillus buchneri E3 is one of LAB isolated during fermentation of the bekasam. Lactobacillus buchneri E3 has antimicrobial activity against pathogen bacteria. The present study aimed to investigate in vivo assessment of antimicrobial activity against Salmonella typhimurium using BALB/c mice. Oral administrations of three doses of L. buchneri E3(108, 109, and 1010 CFU/day/mouse) were performed for seven consecutive days. On the 8th day, each animal was inoculated with a single S. typhimurium, and on the 13th day, the mice were sacrificed for observation. The result showed that oral administration of L. buchneri E3 significantly increased the total population of LAB, significantly decreased S. typhimurium populations in the intestines, liver, and spleen, while increasing beneficial bacterial population and maintaining the normal hematology in the mice. The probiotic also maintained the histological examination of spleen and liver. These suggest that L. buchneri E3 is safe and could be used as the starter for fermentation products.


Akin-Osanaiye, Nok AJ, Amlabu E, Haruna E. 2015. Assessment of changed in serum haematological parameters in the assessment of changed in serum haematological parameters in the plasmodium berghei infected albino mice treated with neem (Azadirachta indica ) extracts. International Journal of Chemical and Biomolecular 1(3) : 148(September):148-152.
Aljahdali NH, Sanad YM, Han J, Foley SL. 2020. Current knowledge and perspectives of potential impacts of Salmonella enterica on the profile of the gut microbiota. BMC Microbiology. 20 : 353
Azad MAK, Sarker M, Wan D. 2018. Immunomodulatory effects of probiotics on cytokine profiles. BioMed Research International. 2018 (8063647)
Bronte V, Pittet MJ. 2013. The spleen in local and systemic regulation of immunity. Immunity. 39(5), 806–818.
Chen L, Deng H, Cui H, Fang J, Zuo Z, Deng J, Li Y, Wang X, Zhao L. 2017. Inflammatory responses and inflammation-associated diseases in organs. Oncotarget, 9(6), 7204–7218.
Eng SK, Pusparajah P, Ab Mutalib NS, Ser HL, Chan KG, Lee LH. 2015. Salmonella: A review on pathogenesis, epidemiology and antibiotic resistance. Frontiers in Life Science. 8: 284–93.
Giles-Gómez M, Sandoval GJG, Matus V, Campos QI, Bolívar F, Escalante A. 2016. In vitro and in vivo probiotic assessment of Leuconostoc mesenteroides P45 isolated from pulque, a Mexican traditional alcoholic beverage. Springerplus. 5(1).
Grace D. 2015. Food safety in low and middle income countries. International Journal of Environmental Research and Public Health 12(9):10490-10507
Hill C, Guarner F, Reid G, Gibson GR, Merenstein DJ, PB. 2014. Expert consensus document: The international scientific association for probiotics and prebiotics consensus statement on the scope and appropriate use of the term probiotic. Nature Reviews Gastroenterology and Hepatology.11(8):506-514
Hilscher M, Sanchez W. 2016. Congestive hepatopathy. Clinical liver disease, 8(3), 68–71.
Hirayama D, Iida T, Nakase H. 2017. The Phagocytic Function of Macrophage-Enforcing Innate Immunity and Tissue Homeostasis. International Journal Molecular Sciences.19(1):92.
La Fata G, Weber P, Mohajeri MH. 2018. Probiotics and the gut immune system: indirect regulation. Probiotics and Antimicrobial Proteins. 10(1):11-21.
Makras L, Triantafyllou V, Fayol-Messaoudi D. 2006. Kinetic analysis of the antibacterial activity of probiotic Lactobacilli towards Salmonellaenterica serovar Typhimurium reveals a role for lactic acid and other inhibitory compounds. Microbiological research.157:241–247.
Mare, AD, Ciurea CN, Man A, Tudor B, Moldovan V, Decean L, Toma F. 2021. Enteropathogenic Escherichia coli—A Summary of the Literature.Gastroenterol. Insights.12, 28–40.
Arcy MAD. 2019. Cell death: a review of the major forms of apoptosis, necrosis and autophagy . Cell Biology International. 43: 582–59
Mikulic J, Bioley G, Corthésy B. 2017. SIga–Shigella immune complexes interact with dectin-1 and SIGNR3 to differentially regulate mouse Peyer's Patch and mesenteric lymph node dendritic cell's responsiveness. Journal of molecular biology. 429 : 2387–2400
Mohamed KF, Ashgan MH, Mohamed ML. (2010). Influence of probiotics mixture on Salmonella typhimurium in mice. International journal of microbiology research.1: 50–61.
Monika MT, Gehlot R., Kumari RA, Priyanka SR. 2021. Antimicrobial properties of probiotics. Environment Conservation Journal. 22 (SE): 33-48.
Nicholson LB. 2016.The immune system. Essays Biochem.60(3):275-301.
Lewis SM, Williams A, Eisenbarth SC. 2019. Structure and function of the immune system in the spleen. Science Immunology. 4: 33
Lhocine N, Arena ET, Bomme P. 2015 Apical invasion of intestinal epithelial cells by Salmonella typhimurium requires villin to remodel the brush border actin cytoskeleton. Cell Host Microbe. 17(2):164-177
Raabe BM, Artwohl JE, Purcell JE, Lovaglio J, Fortman JD. 2011. Effects of weekly blood collection in C57BL/6 mice. Journal of the American Association for Laboratory Animal Science50(5) : 680–685.
Ramirez GA, Yacoub MR, Ripa M, Mannina D, Cariddi A, Saporiti N, Ciceri F, Castagna A, Colombo G, Dagna L. 2018. Eosinophils from physiology to disease: a comprehensive review. BioMed research international. 2018(6): 1-28.
Robinson M, Harmon C, O’Farrelly C. 2016. Liver immunology and its role in inflammation and homeostasis. Cellular & Molecular Immunology. 13 : 267–276.
Shah T, Baloch Z, Shah Z, Cui X, Xia X. 2021. The Intestinal Microbiota: Impacts of Antibiotics Therapy, Colonization Resistance,and Diseases. International Journal of Molecular Science. 22 : 6597
Shu Q, Lin H, Rutherfurd KJ, Gill HS, Rutherfurd KJ, Prasad J. 2000. Dietary bifidobacterium lactis (hn019) enhances resistance to oral Salmonella typhimurium infection in mice. Microbiology and Immunology. 44(3):213-222
Shokryazdan P, Jahromi MF, Liang JB, Kalavathy R, Sieo CC, Ho YW. 2016. Safety assessment of two new Lactobacillus strains as probiotic for human using a rat model. PLOS ONE 11:e0159851.
Steinberg RS, Silva LCS, Souza TC, Lima MT, De Oliveira NLG, Vieira LQ, Arantes RME, Miyoshi A, Nicoli JR, Neumann E, Nunes ÁC. 2014. Safety and Protective Effectiveness of Two Strains of Lactobacillus with Probiotic Features in an Experimental Model of Salmonellosis. International Journal of Environmental Research and Public Health.; 11(9):8755-8776.
Vieco-Saiz N, Belguesmia Y, Raspoet R, Auclair E,2 Gancel F, Kempf I, Drider, 2019. Benefits and Inputs From Lactic Acid Bacteria and Their Bacteriocins as Alternatives to Antibiotic Growth Promoters During Food-Animal Production. Frontiers in Microbiology. 10:57.
Wulandari, E., Yurmiati, H., Subroto, T., Suradi, K. 2020. Quality and probiotic lactic acid bacteria diversity of rabbit meat Bekasam-fermented meat. Journal of Food Science and Animal Resources. 40: 362–76