Screening of indole-3-acetic acid PGPB from three agricultural systems at Nakhon Pathom, Thailand

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DOI https://doi.org/10.13057/biodiv/d231147
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Vol. 23 No. 11 (2022)
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Articles
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This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

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METHANEE HOMTHONG
Division of Biology, Faculty of Science and Technology, Nakhon Pathom Rajabhat University. Nakhon Pathom 73000, Thailand
WANPHEN KAEWPUK
Division of Biology, Faculty of Science and Technology, Nakhon Pathom Rajabhat University. Nakhon Pathom 73000, Thailand
SUKANYA YAMSUAN
Division of Biology, Faculty of Science and Technology, Nakhon Pathom Rajabhat University. Nakhon Pathom 73000, Thailand
ANUNYA THONGSIMA
Division of Biology, Faculty of Science and Technology, Nakhon Pathom Rajabhat University. Nakhon Pathom 73000, Thailand
KANLAYA MOKKAPAN
Expert Center of Innovative Agriculture, Thailand Institute of Scientific and Technological Research. Khlong Luang. Pathumthani 12120, Thailand
VACHIRAPORN PIKULTHONG ♥
Department of Science, Faculty of Science and Technology, Suan Sunandha Rajabhat University. U-Thong nok Rd., Dusit, Bangkok 10300, Thailand

Abstract

Abstract. Homthong M, Kaewpuk W, Yamsuan S, Thongsima A, Mokkapan K, Pikulthong V. 2022. Screening of indole-3-acetic acid PGPB from three agricultural systems at Nakhon Pathom, Thailand. Biodiversitas 23: 5935-5941. Plant growth-promoting bacteria (PGPB) are associated with plant roots and enhance growth via a variety of mechanisms, including fixation of atmospheric nitrogen, solubilization of phosphorus, phytohormone synthesis, and plant phenolic compound stimulation. Sixty-five isolated bacteria from the three agricultural systems in Nakhon Pathom were screened for IAA production. The results showed that seven isolates—C01-28, C02-48, C02-51, G01-34, G03-25, G03-38, and O02-46—produced IAA under L-tryptophan as a precursor at values of 9.08 ± 0.51, 68.64 ± 29.07, 40.67 ± 18.04, 10.65 ± 1.07, 63.30 ± 1.15, 37.73 ± 1.80, and 121.28 ± 15.20 mg mL-1, respectively. Capability on plant growth-promoting traits revealed that none of the isolates stimulated ten-morning glory (Ipomoea aquatica Forsk.) seedling growth, which was not significantly different from the control. Isolates O02-46 enhanced salicylic acid (SA) in ten-morning glory seedlings the most, showing 7.18 ± 1.78 µg g-1 of fresh weight, while isolates G01-34 enhanced phenolic compound (PC) production the most showing 218.18 ± 29.55 mg GAE g-1 extract, respectively. The 16S rRNA analysis revealed that isolates O02-46, C02-48, G03-25, and C02-51 were identified as similar to Paenibacillus alvei at 99.20% (Accession number AY826588.1), Bacillus megaterium at 79.91% (Accession number MH031358.1), Bacillus sp. at 98.57% (Accession number JF322976.1), and Lysinibacillus fusiformis at 99.61% (Accession number HE610782.1).

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