Determination of phytochemical constituents, antibacterial and antioxidant activities of ethanolic leaf extracts of Pterocarpus erinaceus
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Abstract
Abstract. Okoli EC, Umaru IJ, Olawale O. 2023. Determination of phytochemical constituents, antibacterial and antioxidant activities of ethanolic leaf extracts of Pterocarpus erinaceus. Biodiversitas 24: 2272-2277. Many people in Nigeria and West Africa have long used the leaves, stem bark, and roots of Pterocarpus erinaceus Poir. as a medicine to treat ailments such as malaria, ulcers, coughs, and fevers. The antioxidant, antimalarial, antiulcer, and antibacterial activities of stem bark, leaves, and roots of P. erinaceus have been investigated. The aim of this study was to determine the phytochemical composition, antimicrobial activity, and antioxidant activity of the P. erinaceus leaf. Phytochemical compounds in ethanolic leaf extracts of P. erinaceus were analyzed and quantified by Gas Chromatography/Flame Ionization Detector (GC-FID). The antibacterial test was performed against Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, and Escherichia coli by agar diffusion. The antioxidant activity was evaluated by DPPH scavenging activity. Phytochemicals content in the extract include flavonoids (44.71%), alkaloids (19.47%), steroids (6.01%), tannins (1.73%), saponins (4.70%), glycosides (7.00%), tannins (2.60%), anti-nutrient (12.77%). Ethanol extracts of P. erinaceus leaves were effective against all selected bacterial strains (E. coli, S. aureus, B. subtilis, and P. aeruginosa). DPPH scavenging activity of the ethanol extract of P.erinaceus at a concentration of 1000 ?g/mL was 49.51% and 27.20% at 40 ?g/mL, categorized as moderate antioxidant activity. This study showed that crude ethanol extracts of P. erinaceus leaf contained pharmacologically active compounds and exhibited significant antibacterial and DPPH free radical scavenging activities in a concentration-dependent manner.
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References
Ahmed, M.U., Modibbo, A.A., Zaruwa, M.Z. and Sudi, I.Y., 2017. Phytochemical and Nutrient Composition of Pterocarpus erinaceus Stem Bark. International Journal of Biochemistry Research and Review, 19:1-6. DOI: 10.9734/IJBCRR/2017/36546
Alves de Almeida, A.C., de-Faria, F.M., Dunder, R.J., Manzo, L.P.B., Souza-Brito, A.R.M. and Luiz-Ferreira, A., 2017. Recent trends in pharmacological activity of alkaloids in animal colitis: potential use for inflammatory bowel disease. Evidence-based complementary and alternative medicine, 2017: 1 24 https://doi.org/10.1155/2017/8528210
Bhattacharyya, A., Chattopadhyay, R., Mitra, S. and Crowe, S.E., 2014. Oxidative stress: an essential factor in the pathogenesis of gastrointestinal mucosal diseases. Physiological reviews, 94(2): 329-354. DOI:10.1152/physrev.00040.2012
Chen, A.Y. and Chen, Y.C., 2013. A review of the dietary flavonoid, kaempferol on human health and cancer chemoprevention. Food chemistry, 138(4): 2099-2107. https://doi.org/10.1016/j.foodchem.2012.11.139
Chung, K.T., Wong, T.Y., Wei, C.I., Huang, Y.W. and Lin, Y., 1998. Tannins and human health: a review. Critical reviews in food science and nutrition, 38(6): 421-464. https://doi.org/10.1080/10408699891274273
Ertop, M.H. and Bekta?, M., 2018. Enhancement of bioavailable micronutrients and reduction of antinutrients in foods with some processes. Food and Health, 4(3): 159-165. DOI: 10.3153/FH18016
Ezeja, I. M., Ezeigbo, I. I., Madubuike, K. G., Udeh, N. E., Ukweni, I. A., Akomas, S. C., & Ifenkwe, D. C. (2012). Antidiarrheal activity of Pterocarpus erinaceus methanol leaf extract in experimentally-induced diarrhea. Asian Pacific journal of tropical medicine, 5(2): 147–150. https://doi.org/10.1016/S1995-7645(12)60014-5
Franck, X., Fournet, A., Prina, E., Mahieux, R., Hocquemiller, R. and Figadère, B., 2004. Biological evaluation of substituted quinolines. Bioorganic & medicinal chemistry letters, 14(14): 3635 3638. https://doi.org/10.1016/j.bmcl.2004.05.026
Gabriel, A.F. and Onigbanjo, H.O., 2010. Phytochemical and antimicrobial screening of the stem bark extracts of Pterocarpus erinaceus (Poir). Nigerian Journal of Basic and Applied Sciences, 18(1): 1-5. DOI: 10.4314/njbas.v18i1.56835
Galluzzo, P., Ascenzi, P., Bulzomi, P. and Marino, M., 2008. The nutritional flavanone naringenin triggers antiestrogenic effects by regulating estrogen receptor ?-palmitoylation. Endocrinology, 149(5): 2567-2575. https://doi.org/10.1210/en.2007-1173
Ganeshpurkar, A. and Saluja, A.K., 2017. The pharmacological potential of rutin. Saudi pharmaceutical journal, 25(2): 149-164. https://doi.org/10.1016/j.jsps.2016.04.025
Hossain, M.M., Uddin, M.S., Baral, P.K., Ferdus, M. and Bhowmik, S., 2022. Phytochemical screening and antioxidant activity of Ipomoea hederifolia stems: A potential medicinal plant. Asian Journal of Natural Product Biochemistry, 20(2): 41-47 DOI: 10.13057/biofar/f200201
Isaac John Umaru, Ebenezer Morayo Ale, Imaranezor Kenneth, Bilyaminu Habibu, Muhammad Zuhairah Ismail, Okoli Chikodiri Emmanuel, Yusuf Dawoye and Barrah Collins Chizaram, 2022. Antidiarrheal and Antibacterial Actions of Methanol Extract of Cassia fistula (Linn) Leaves. Journal of Applied Sciences, 22(6): 279-287. DOI: 10.3923/jas.2022.279.287
Isemura, M., 2019. Catechin in human health and disease. Molecules, 24(3): 1 5. https://doi.org/10.3390/molecules24030528
Jiang, N., Doseff, A.I. and Grotewold, E., 2016. Flavones: from biosynthesis to health benefits. Plants, 5(2): 1-25. https://doi.org/10.3390/plants5020027
Karou, D., Dicko, M.H., Sanon, S., Simpore, J. and Traore, A.S., 2003. Antimalarial activity of sida acuta burm. F.(Malvaceae) and Pterocarpus erinaceus poir.(Fabaceae). Journal of ethnopharmacology, 89(2-3): 291-294. DOI: 10.1016/j.jep.2003.09.010
Lewis, K. and Ausubel, F.M., 2006. Prospects for plant-derived antibacterials. Nature biotechnology, 24(12): 1504-1507. DOI: 10.1038/nbt1206-1504
M Calderon-Montano, J., Burgos-Morón, E., Pérez-Guerrero, C. and López-Lázaro, M., 2011. A review on the dietary flavonoid kaempferol. Mini reviews in medicinal chemistry, 11(4): 298 344. https://doi.org/10.2174/138955711795305335
Mastroeni, P., 2002. Immunity to systemic Salmonella infections. Current molecular medicine, 2(4): 393-406. DOI: 10.2174/1566524023362492
Noufou, O., Andre, T., Richard, S.W., Yerbanga, S., Maminata, T., Ouedraogo, S., Anne, E.H., Garcia, I. and Pierre, G.I., 2016. Anti-inflammatory and Anti-plasmodial Activities of Methanol Extract of Pterocarpus erinaceus Poir.(Fabaceae) Leaves. International Journal of Pharmacology, 12(5): 549 555. DOI: 10.3923/ijp.2016.549.555
Noufou, O., Wamtinga, S.R., André, T., Christine, B., Marius, L., Emmanuelle, H.A., Jean, K., Marie-Geneviève, D. and Pierre, G.I., 2012. Pharmacological properties and related constituents of stem bark of Pterocarpus erinaceus Poir.(Fabaceae). Asian Pacific Journal of Tropical Medicine, 5(1): 46 51. https://doi.org/10.1016/S1995-7645(11)60244-7
Olaleye, M.T., Akinmoladun, A.C., Crown, O.O., Ahonsi, K.E. and Adetuyi, A.O., 2013. Homopterocarpin contributes to the restoration of gastric homeostasis by Pterocarpus erinaceus following indomethacin intoxication in rats. Asian Pacific Journal of Tropical Medicine, 6(3): 200-204. DOI: 10.1016/S1995-7645(13)60023-1
Paul, R., Prasad, M. and Sah, N.K., 2011. Anticancer biology of Azadirachta indica L (neem): a mini review. Cancer biology & therapy, 12(6): 467-476. DOI:10.4161/cbt.12.6.16850
Popova, A. and Mihaylova, D., 2019. Antinutrients in plant-based foods: A review. The Open Biotechnology Journal, 13(1): 68-76 DOI: 10.2174/1874070701913010068
Prakash, M., Basavaraj, B.V. and Murthy, K.C., 2019. Biological functions of epicatechin: Plant cell to human cell health. Journal of functional foods, 52:14-24. https://doi.org/10.1016/j.jff.2018.10.021
Saslis-Lagoudakis, C.H., Klitgaard, B.B., Forest, F., Francis, L., Savolainen, V., Williamson, E.M. and Hawkins, J.A., 2011. The use of phylogeny to interpret cross-cultural patterns in plant use and guide medicinal plant discovery: an example from Pterocarpus (Leguminosae). PloS one, 6(7): e22275. https://doi.org/10.1371/journal.pone.0022275
Schlemmer, U., Frølich, W., Prieto, R.M. and Grases, F., 2009. Phytate in foods and significance for humans: food sources, intake, processing, bioavailability, protective role and analysis. Molecular nutrition & food research, 53(S2): 330-375. https://doi.org/10.1002/mnfr.200900099
Seeram, N.P., Schutzki, R., Chandra, A. and Nair, M.G., 2002. Characterization, quantification, and bioactivities of anthocyanins in Cornus species. Journal of agricultural and food chemistry, 50(9): 2519-2523. DOI: 10.1021/jf0115903
Sharma, K., Kumar, V., Kaur, J., Tanwar, B., Goyal, A., Sharma, R., Gat, Y. and Kumar, A., 2021. Health effects, sources, utilization and safety of tannins: A critical review. Toxin Reviews, 40(4): 432 444. https://doi.org/10.1080/15569543.2019.1662813
Singh, R.P., Chidambara Murthy, K.N. and Jayaprakasha, G.K., 2002. Studies on the antioxidant activity of pomegranate (Punica granatum) peel and seed extracts using in vitro models. Journal of agricultural and food chemistry, 50(1): 81-86. DOI: 10.1021/jf010865b
Sultana, B., Anwar, F. and Przybylski, R., 2007. Antioxidant activity of phenolic components present in barks of Azadirachta indica, Terminalia arjuna, Acacia nilotica, and Eugenia jambolana Lam. trees. Food chemistry, 104(3): 1106-1114. https://doi.org/10.1016/j.foodchem.2007.01.019
Tittikpina, N.K., Nana, F., Fontanay, S., Philippot, S., Batawila, K., Akpagana, K., Kirsch, G., Chaimbault, P., Jacob, C. and Duval, R.E., 2018. Antibacterial activity and cytotoxicity of Pterocarpus erinaceus Poir extracts, fractions and isolated compounds. Journal of Ethnopharmacology, 212: 200-207. DOI: 10.1016/j.jep.2017.10.020
Tittikpina, N.K., Sandjo, L.P., Nana, F., Vaillant, V., Fontanay, S., Philippot, S., Diop, Y.M., Batawila, K., Akpagana, K., Kirsch, G. and Duval, R.E., 2019. Investigation of the antifungal activity of Pterocarpus erinaceus led to the identification of two new diarylpropanoids from its stem barks. Phytochemistry Letters, 32: 110-114. https://doi.org/10.1016/j.phytol.2019.05.013
Ugoeze, K.C., Oluigbo, K.E. and Chinko, B.C., 2020. Phytomedicinal and Nutraceutical Benefits of the GC-FID Quantified Phytocomponents of the Aqueous Extract of Azadirachta indica leaves. Journal of Pharmacy and Pharmacology Research, 4(4): 149-163. DOI: 0.26502/fjppr.039