First report of Enterobacter sp. causing bacterial wilt on patchouli in Aceh, Indonesia

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ZULFADLI
NURAINUN INTAN WASISTHA
HARTATI OKTARINA
KHAIRAN
RINA SRIWATI

Abstract

Abstract. Zulfadli, Wasistha NI, Oktarina H, Khairan, Sriwati R. 2023. First report of Enterobacter sp. causing bacterial wilt on patchouli in Aceh, Indonesia. Biodiversitas 24: 3814-3820. Patchouli (Pogostemon cablin Benth), is an essential commodity in Indonesia, with Aceh Jaya being one of its main producing regions. Several incidences of wilt disease symptoms have been reported in patchouli plantations in Ranto Sabon, Sampoianet Sub-district, Aceh Jaya District. This study aimed to isolate and identified the bacterial pathogen responsible for wilt disease in patchouli in Aceh Jaya. Various tests, including morphology characterization and molecular characteristics using 16s PCR primer, hypersensitive test, and pathogenicity test were conducted to identify the cause of disease. The isolation result revealed that Enterobacter sp. was responsible for invading the patchouli plant. Further testing was carried out on various culture media including Nutrient Broth, Tryptic Soy, Kings B, Sulfide Indole Motility, Sucrose Potato Agar, and Yeast Potato Agar. The highest colony density was found in Tryptic Soy media (2.002) after 120 hours of inoculation. The results of hypersensitivity and pathogenicity tests showed that Enterobacter sp. caused symptoms similar to those of natural infection. Among all the media, Sucrose Potato Agar media had the fastest incubation period, with 7 and 48 days after inoculation. Therefore, this is the first study to report the presence of Enterobacter sp. associated with bacterial wilt in patchouli in Aceh.

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References
Amrulloh MK, Addy HS and Wahyuni WK. 2021. Karakterisasi Fisiologis dan Biokimia Penyebab Penyakit Bakteri Pembuluh Kayu pada Tanaman Cengkeh (Syzygium aromaticum L.) di PT Tirta Harapan. .Jurnal Proteksi Tanaman Tropis 2: 1–7. DOI: 10.19184/jptt.v2i1.17919.
Aruwa CE. 2017. Microbiological Quality Assessment of Pupuru and Plantain Flours in An Urban Market Akure, Ondo State, South Western Nigeria. Open Access Library Journal 4: 1-11. DOI: 10.4236/oalib.1103783.
Asman A, Nasrun A, Nurawan and Sitepu D. 1993. Penelitian Penyakit Tanaman Nilam. Risalah Kongres Nasional XII dan Seminar Ilmiah PFI Yogyakarta 2: 903–911.
Asrul, Arwiyanto T, Hadisutrisno B, Widada J and Mollah A. 2021. First Report of Enterobacter cloacae in Shallot (Allium cepa L. Aggregatum group) in Indonesia. IOP Conference Series: Earth and Environmental Science 681: 1–10. DOI: 10.1088/1755-1315/681/1/012048.
Barus T. 2019. Identifikasi dan Keragaman Genetik Bakteri Asam Laktat dari Tapai Singkong Berdasarkan Sekuen Gen 16S rRNA. Biota: Jurnal Ilmiah Ilmu Ilmu Hayat 2: 46–52.DOI: 10.24002/biota.v2i2.1656.
Buddenhagen IW and Kelman A. 1964. Biological and Physiological Aspects of Bacterial Wilt Caused by Pseudomonas solanacearum. Annu Rev Phytopathol 2: 203–230. DOI: 10.1146/annurev.py.02.090164.001223.
Cappucino JG and Sherman N. 2013. Microbiologi, A laboratory Manual. California (UK): Benjamin Cummings.
Ditjenbun, 2020. Harumnya Nilam Primadona Dunia. https://ditjenbun.pertanian.go.id/ harumnya-nilam-primadona-dunia/.
Duveiller E, Bragard C, Rudolph K and Fucikovsky L. 1997. General Concepts and Methods for the Identification of Pathogenic Bacteria of Wheat. Mexico: CIIMYT.
Hartati SY and Nuri K. 2014. Teknik Inokulasi Ralstonia solanacearum Untuk Pengujian Ketahanan Nilam Terhadap Penyakit Layu. Buletin Penelitian Tanaman Rempah Dan Oba 25: 12–18. DOI: 10.21082/bullittro.v25n2.2014.127-135.
Hayward AC. 1960. A Method for Charazterizing Pseudomonas solanacearum. Nature 186: 405–406.
Maulidia V, Sriwati R, Soesanto L, Syamsuddin, Hamaguchi T and Hasegawa K. 2021. Endophytic Bacteria Isolated from Higher Plant in Aceh, Indonesia, and Their Chemical Compounds Activity against Fusarium oxysporum f. sp. lycopersici. Egyption Journal of Biological Pest Control 31: 1-7. DOI: 10.1186/s41938-021-00379-5.
Mustika I, Rachmat A and Suyanto D. 1995. Pengaruh Pupuk, Pestisida, Bahan Organik, terhadap pH Tanah, Populasi Nematoda dan Produksi Nilam. Media Komunikasi Penelitian Dan Pengembangan Tanaman Industri 15: 70–74.DOI: 10.21082/bullittro.v23n1.2012.%25p.
Nuryani Y. 2006. Budidaya Tanaman Nilam (Pogostemon cablin Benth). Bogor: Balai Penelitian Tanaman Rempah dan Obat Departemen Pertanian.
Oberman H and Libudzisz Z. 1998. Fermented Milks. In: BJB Wood, ed. Microbiology of Fermented Foods. London: Blackie Academic and Professional.
Pelczar MJ and Chan ECS. 1986. Dasar - Dasar Mikrobiologi I. Jakarta: Universitas Indonesia.
Pradana RS. 2020. Peranan Tenaga Kerja dan Investasi Terhadap Produksi Industri Penyulingan Nilam Di Kabupaten Aceh Jaya. Jurnal Agrica 13: 1–9. DOI: 10.31289/agrica.v13i1.3105.
Sanders JW and Sanders CC. 1997. Enterobacter spp.: Pathogens Poised To Flourish at the Turn Of The Century. Clint Microbiol Rev 10: 220–241.DOI: 10.1128/CMR.10.2.220.
Santana MA, Rodriguez M, Mathehus J, Faks J, Bocsanczy A, Gerstl A, Romay G, Montilla J, Fernánde CE. Moreno-Zambrano N and Marvall D. 2012. A New Bacterial Disease of Cassava in Venezuela Caused by Enterobacter cloacae. Int J Agric Biol 4: 183–189.
Sarkar R and Chaudhuri S. 2015. New Report of Additional Enterobacterial Species Causing Wilt in West Bengal, India. Can J Microbiol 61: 477–486. DOI: 10.1139/cjm-2015-0017.
Sastrohamidjojo H. 2005. Kimia Organik. Yogyakarta: Gadjah Mada University Press.
Schaad NW, Jones JB and Chun W. 2001. Laboratory Guide of Plant Patthogenic Bacteria. (Third Edi ed. Minnesota, USA: The American Phytopathological Society (APSPRESS).
Sogandi, 2018. Biologi Molekuler: Identifikasi Bakteri secara Molekuler. Jakarta Utara: Universitas 17 Agustus 1945.
Stackebrant E and Goebel BM. 1994. Taxonomic Note: A Place for DNA-DNA Reassociation and 16S rRNA Sequence Analysis in the Present Species Definition in Bacteriology. International Journal of Systematic and Evolutionary Microbiology 44: 846–849. DOI: 10.1099/00207713-44-4-846.
Supriadi, Rizal M and Wahyuno D. 2011. Nilam (Pogostemon cablin Benth): Status Teknologi Hasil Penelitian Nilam. Bogor: Balai Penelitian Tanaman Obat dan Aromatik.
Walker L, Harry L and Mathias J. 2006. Koch’s Postulates and Infectious Proteins. Acta Neuropathologica 112: 1–4. DOI: 10.1007/s00401-006-0072-x.
Wang GF, Kawicha P, Xie GL and Zhu B. 2008. Bacterial Wilt of Mulberry ( Morus alba ) Caused by Enterobacter cloacae in China. Plant Disease 92: 483-483. DOI: 10.1094/PDIS-92-3-0483B.
Wu J, Ding Z, Diao Y and Hu Z. 2011. First Report On Enterobacter sp. Causing Soft Rot of Amorphophallus konjac in China. Journal of General Plant Pathology 77: 312-314. DOI: 10.1007/s10327-011-0330-1.
Wu W, Feng Y and Zong Z. 2020. Precise Species Identification for Enterobacter?: a Genome Sequence-Based Study with Reporting of Two Novel Species, Enterobacter quasiroggenkampii sp. nov. and Enterobacter quasimori sp. nov. mSystems 5: 1–16. DOI: 10.1128/msystems.00527-20.
Zhang ZF and Nan ZB. 2013. Occurrence of Lucerne Seedborne Enterobacter cloacae Sprouts Decay in Gansu Province of China. Eur J Plant Pathol 135: 5–9. DOI: 10.1007/s10658-012-0072-8.

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