Anatomical, morphological and physiological leaf characters of black betel (Piper betle L. var. nigra) in varying natural and man-made habitats

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PARAMITA CAHYANINGRUM KUSWANDI
NUR AENI ARIYANTI
MUHAMAD FAHMI YUNUS
CHE NURUL AINI CHE AMRI

Abstract


Abstract. Kuswandi PC, Ariyanti NA, Yunus MF, Amri CNAC. 2023. Anatomical, morphological and physiological leaf characters of black betel (Piper betleL. var. nigra) in varying natural and man-made habitatsBiodiversitas 243236-3244Piper betle L. var. nigra or black betel (known as Sirih hitam in Indonesia) contains valuable secondary metabolites, such as alkaloids, flavonoids, saponins, tannins, phenols, carotenoids, steroids and terpenoids. Black betel leaf extract has been shown to have antimicrobial activity thus there is a prospect to be developed as a promising herbal plant. Nevertheless, little information is available about its development as a medicinal plant. This research studies the influence of different habitats on several characters of black betel leaves with the aim to understand the suitable environmental conditions for the optimum growth of black betel plants. We used a survey method and random sampling of black betel leaves in four locations in Java Island, Indonesia, namely Banyuwangi which represents natural habitat, and Karanganyar, Ngaglik and Pakem which represent man-made habitats. Measurements of temperature, humidity, soil moisture and light intensity were carried out at each location. Analyses of leaf area, leaf water content, total leaf chlorophyll content and flavonoid content were undertaken and statistically analyzed using SPSS software. Leaf transverse sections were also observed. The results showed that the environmental parameters differed in the four locations. Leaves samples from the natural habitat in Banyuwangi were significantly different (P<0.05) from the three man-made habitats for chlorophyll and flavonoid content. For water content, significant difference was only for Banyuwangi samples with those from Karanganyar and Pakem. For leaf area, significant difference was only found between the Banyuwangi samples and Karanganyar. Observations on the transverse cross section of midrib of black betel leaves from the four locations showed structures that are generally found in Piper betle species, namely the presence of an epidermal layer, trichomes in the abaxial part of the leaf, several layers of the hypodermis, visible vascular tissue and the presence of secretion cells. There were several differences in the leaf anatomy such as greater number of trichomes on the leaves from Karanganyar, the secretory cells that were more visible in the leaves from Ngaglik and Banyuwangi and the sclerenchymal tissue that was more visible in the leaves from Banyuwangi. Such differences are likely influenced by variations in environmental parameters thus showing that the man-made habitat in the Karanganyar location can affect leaves characters similar to black betel plant grown in its natural habitat in Banyuwangi.


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References
Arnon D. 1949. Copper enzymes in isolated chloroplasts polyphenoloxidase in Beta Vulgaris. Plant Physiology 24 (1) :1-15. DOI : 10.1104/pp.24.1.1
Chen D, Melton LD, Zujovic Z. et al. 2019. Developmental changes in collenchyma cell-wall polysaccharides in celery (Apium graveolens L.) petioles. BMC Plant Biol 19 (18). DOI : 10.1186/s12870-019-1648-7
Chen JJ, Sun Y, Kopp K, Oki L, Jones SB, Hipps L. 2022. Effects of Water Availability on Leaf Trichome Density and Plant Growth and Development of Shepherdia utahensis. Frontier of Plant Science. 18; 13:855858. DOI: 10.3389/fpls.2022.855858.
Dewi GP, Kuntorini EM, Pujawati ED. 2020. Struktur Anatomi dan Uji Histokimia Terpenoid dan Fenol Dua Varietas Sirih Hijau (Piper betle L.). Bioscientiae 17(2), 1-14. DOI: 10.20527/b.v17i2.3448
Fender AC, Mantilla-Contreras J, Leuschner C. 2011. Multiple environmental control of leaf area and its significance for productivity in beech saplings. Trees 25: 847–857. DOI: 10.1007/s00468-011-0560-z
Harjunowibowo D, Yudi Rinanto. 2020. Penerapan Parameter Habitat Hutan Raung pada Pembiakan Sirih Hitam (Black Piper Betle L.) Laporan Akhir Pengabdian Kepada Masyarakat (Pkm) Kerjasama Dengan Umkm Nyawiji Agriraya. LPPM UNS.
Hu Y, Yang L, Gao C, Liao D, Long L, Qiu J, Wei H, Deng Q, Zhou Y. 2022. A comparative study on the leaf anatomical structure of Camellia oleifera in a low-hot valley area in Guizhou Province, China. PLOS ONE 17(1): e0262509. DOI : 10.1371/journal.pone.0262509
Idris A, Alona L, Mohd Fadzelly AB, Zakiyyu T. 2018. Effect of Light Quality and Quantity on the Accumulation of Flavonoid in Plant Species. Journal of Science and Technology 10: 32-45. DOI : 10.30880/jst.2018.10.03.006..
Jarvis MC. 2012. Sclerenchyma In: Encyclopedia of Life Sciences. John Wiley & Sons Ltd: Chichester. DOI: 10.1002/9780470015902.a0002082. pub2
Jin B, Wang L, Wang J, Zhen JK, Wang Y, Xue JX, Yang NC, Long WY, Jun TN. 2011. The effect of experimental warming on leaf functional traits, leaf structure and leaf biochemistry in Arabidopsis thaliana. BMC Plant Biol 11: 35. DOI: 10.1186/1471-2229-11-35
Jumrani K, Bhatia VS, Pandey GP. 2017. Impact of elevated temperatures on specific leaf weight, stomatal density, photosynthesis and chlorophyll fluorescence in soybean. Photosynth Res 131: 333–350. DOI: 10.1007/s11120-016-0326-y
Junairiah, Zuraidassanaaz NI, Izdihar FN, Manuhara YSW. 2017.Callus Induction of Leaf Explant Piper betle L. Var Nigra with Combination of Plant Growth Regulators Indole-3-Acetic Acid(IAA), Benzyl Amino Purin (BAP) and Kinetin. AIPConference Proceedings1888, 020028. 6th International Conference and Workshops on Basic and Applied Sciences, ICOWOBAS 2017 - Erbil, Kurdistan, Iraq. 18-19 Mar 2017.
Junairiah, Mahmuda A, Manuhara YSW, Ni’matuzahroh, Sulistyorini. 2019. Callus Induction and Bioactive Compounds from Piper betle L. Var Nigra. IOP Conf. Series: Earth and Environmental Science. DOI: 10.1088/1755-1315/217/1/012026
Junairiah, Ni’matuzahroh, Zuraidassanaaz NI, Sulistyorini L. 2018a. Isolation and Identification of Secondary Metabolites of Black Betel (Piper betle L. var Nigra). Jurnal Kimia Riset 3 (2): 131-138. DOI : 10.20473/jkr.v3i2.12064
Junairiah, Purnomo, Utami ESW, Ni’matuzahroh, Sulistyorini L. 2018b. Callus Induction of Piper betle Var Nigra Using 2,4-Dichlorofenoxyacetic Acid and 6-Benzil Aminopurin. Biosaintifika 10 (3): 588-596. DOI : 10.15294/biosaintifika.v10i3.15962
Kim HJ, Seo EY, Kim J H, Cheong HJ, Kang BC, Choi DI. 2012. Morphological classification of trichomes associated with possible biotic stress resistance in the genus Capsicum. The Plant Pathology Journal, 28(1): 107-113. DOI : 0.5423/PPJ.NT.12.2011.0245
Li AM, Li SH, Wu XJ, Zhang J, He AN, Zhao G, Yang X. 2016. Effect of Light Intensity on Leaf Photosynthetic Characteristics and Accumulation of Flavonoids in Lithocarpus litseifolius(Hance) Chun. (Fagaceae). Open Journal of Forestry 6: 445-459. DOI : 10.4236/ojf.2016.65034
Li Y, He N, Hou J, Xu L, Liu C, Zhang J, Wang Q, Zhang X, Wu X. 2018. Factors Influencing Leaf Chlorophyll Content in Natural Forests at the Biome Scale. Frontiers in Ecology and Evolution 6:64. DOI: 10.3389/fevo.2018.00064
Muttaleb QA, Abdullah TL, Hassan SA, Rashid AA, Taheri S, Ahmed OA, Abdulameer DA. 2018. The Role of Shade and Nitrogen on Physiological Traits and Secondary Metabolites of Piper betle L. Journal of Horticulture 05 (2). DOI : 10.4172/2376-0354.1000230.
Nugroho AW. 2017. Konservasi Keanekaragaman Hayati Melalui Tanaman Obat Dalam Hutan Di Indonesia Dengan Teknologi Farmasi: Potensi Sains Dan Tantangan. Jurnal Kesehatan 1(7): 377-383. DOI : 10.25026/jsk.v1i7.71
Nugroho RA, Ningsih EA. 2017. Produksi Tanaman Obat. In: Z.Salim dan E.Munadi (eds). Info Komoditi Tanaman Obat ). Balai Pengkajian dan Pengembangan Perdaganagan Kementrian Perdagangan Republik Indonesia, Jakarta.
Nurul-Aini CAC, Nur-Shuhada T, Rozilawati S, Fatin MA, Noraini T, Latiff M A. 2018. Comparative leaf anatomy of selected medicinal plants in Acanthaceae. International Medical Journal Malaysia, 17(2): 17-24. DOI : 10.31436/imjm.v17i2.944
Novita W. 2016. Uji Aktivitas Antibakteri Fraksi Daun Sirih (Piper Betle L) Terhadap Pertumbuhan Bakteri Streptococcus Mutans Secara In Vitro. Jambi Medical Journal "Jurnal Kedokteran Dan Kesehatan" 4(2). DOI : 10.22437/jmj.v4i2.3579
Perangin-Angin Y, Purwaningrum Y, Asbur MS, Rahayu, Nurhayati. 2019. Pemanfataan kandungan metabolit sekunder yanag dihasilkan tanaman pada cekaman biotik. Agriland 7 (1) : 39-47. DOI : 10.30743/agr.v7i1.3471
Prasetya F, Salam S, Rahmadani A, Haikal K, Febrina L, Anshory H, Arifuddin M, Siregar V O, Narsa AC, Herman H, Ahmad I, Indriyanti N, Ibrahim A, Rusli R, Rijai L, Kuncoro H. 2021a. Novel amides derivative with antimicrobial activity of Piper betle var. Nigra leaves from Indonesia. Molecules 26(2): 1–8. DOI: 10.3390/molecules26020335
Prasetya F, Supriatno S, Rahmadani A, Kuncoro H, Rusli R. 2021b. Chemical constituents from Piper betle L. var. Nigra (Piperaceae). Journal of Tropical Pharmacy and Chemistry 5(3): 229–232. DOI: 10.25026/jtpc.v5i3.322
Queiroz GS, Pedro O, Rosilene S, George C, Larissa. 2018. Drying temperature changes trichome integrity, chemical content and composition of the essential oil of pepper-rosmarin. Ciência Rural 48 (11): e20180283. DOI: 10.1590/0103-8478cr20180283
Raman V, Galal A, Khan, I. 2012. An Investigation of the Vegetative Anatomy of Piper sarmentosum, and a Comparison with the Anatomy of Piper betle (Piperaceae). American Journal of Plant Sciences 3: 1135-1144. DOI: 10.4236/ajps.2012.38137.
Rodríguez VM, Soengas P, Alonso-Villaverde V, Sotel, T, Cartea ME, Velasco P. 2015. Effect of temperature stress on the early vegetative development of Brassica oleracea L.. BMC Plant Biol 15: 145. DOI: 10.1186/s12870-015-0535-0
Rudall JP. 2020. Anatomy of Flowering Plant. An Introduction to Plant Structure and Development. (4th Ed.). Cambridge University Press. Cambridge. Sari EF, Prayogo GP, Loo YT, Zhang P, McCollough MJ, Cirillo N. 2020. Distinct phenolic, alkaloid and antioxidant profile in betel quids from four regions of Indonesia. Sci Rep 10: 16254. DOI: 10.1038/s41598-020-73337-0
Susilo DEH. 2015. Identification of Constanta Value of Leaf Shape for Leaf Area Measurement Using Length Cross Width of Leaf of Horticulture Plant in Peat Soil. Anterior Jurnal 14(2): 139–146. DOI: 10.33084/anterior.v14i2.178
Suriani NL, Suprapta DN, Nazir N, Parwanayoni NMS, Darmadi AAK, Dewi DA, Sudatri NW, Fudholi A, Sayyed RZ, Syed A, Elgorban AM, Bahkali AH, Enshasy HA, Dailin DJ. 2020. A Mixture of Piper Leaves Extracts and Rhizobacteria for Sustainable Plant Growth Promotion and Bio-Control of Blast Pathogen of Organic Bali Rice. Sustainability 12 (20): 8490. DOI: 10.3390/su12208490
Wang Z, Huang H, Wang H. et al. 2022. Leaf water content contributes to global leaf trait relationships. Nat Commun 13: 5525. DOI: 10.1038/s41467-022-32784-1
Weraduwage WS, Chen J, Anozie FC, Morales A, Weise SE, Sharkey TD. 2015. The relationship between leaf area growth and biomass accumulation in Arabidopsis thaliana. Frontiers in Plant Science 6. DOI: 10.3389/fpls.2015.00167.
Yuanyue L, Zhongbao L, Peng L. 2009. The study on the leaf anatomy of some mangrove species of China. International Conference on Environmental Science and Information Application Technology 3: 47-51. DOI 10.1109/ESIAT.2009.397
Zhang H, Zhong H, Wang Ji, Sui X, Xu N. 2016. Adaptive changes in chlorophyll content and photosynthetic features to low light in Physocarpus amurensis Maxim and Physocarpus opulifolius “Diabolo.” PeerJ 4: e2125. DOI: 10.7717/peerj.2125.