Morphological, phytochemical, and molecular profiling of bamboo species (Bambuseae) growing in various ecosystems of Cagayan Province, Luzon, Philippines




Abstract. Opeña JM, Bumanglag RA, Cabang VMT. 2023. Morphological, phytochemical, and molecular profiling of bamboo species (Bambuseae) growing in various ecosystems of Cagayan Province, Luzon, Philippines. Biodiversitas 24: 4342-4358. The characterization of bamboo species posed advantages to the bamboo industry’s development in the Philippines. This study aimed to provide baseline information on the environmental and biological profiles of bamboo species in Cagayan Province, Philippines. Study sites were visited for bamboo morphological characterization using character states. Leaves were collected for qualitative phytochemical screening of 10 secondary metabolites and DNA sequencing using the rbcL plastid gene. The 28 accessions of Bambusa, Dendrocalamus, Dinochloa, Gigantochloa, Guadua, Melocanna, Phyllostachys, Schizostachyum, and Thyrsostachys were characterized and these bamboos were growing in forests, coastal or sparsely vegetated lands, urban, freshwater, grasslands, and agricultural ecosystems. Bamboo species distinctiveness’ was described through identification keys on growth habit, culm internode, nodal structure, young shoot, and flowering incidence. The bamboos contain coumarins, saponins, steroids, and terpenoids, while quinones are absent. DNA sequencing using rbcL was effective in the identification of Bambusa vulgaris species and unknown bamboo at the species level. Dendrocalamus, Guadua, Melocanna, and Schizostachyum species were successfully identified by rbcL at the genus level. Most of the species were identified as Dendrocalamus species with rbcL. The findings provide valuable information on species identification and certification for asexual propagation, genetic conservation, phytochemical extraction, and utilization for health.


Aguinsatan RG, Razal RA, Carandang MG, Peralta EK. 2019. Site influence on the morphological, physical and mechanical properties of giant bamboo (Dendrocalamus asper) in Bukidnon Province, Mindanao, Philippines. Journal of Tropical Forest Science 31(1): 99-107. DOI: 10.26525/jtfs2019.31.1.099107.
Baguistan BJ, Waing KG, Valentino MJ. 2017. Phytochemical screening and determination of the biological activities of Bambusa vulgaris var. striata and Dendrocalamus asper shoot extracts. International Journal of Biology, Pharmacy and Allied Sciences 6(11): 2109-2119.
Bedoya LM, Beltrán M, Sancho R, Olmedo DA, Sánchez-Palomino S, del Olmo E, Lopez-Perez JL, Muñoz E, San Feliciano A, Alcamí J. 2005. 4-Phenylcoumarins as HIV transcription inhibitors. Bioorganic & Medicinal Chemistry Letters15(20): 4447-4450. DOI: 10.1016/j.bmcl.2005.07.041.
Bennet SSR, Gaur RC. 1990. Thirty seven bamboos growing in India. Forest Research Institute, Simla, India.
Bidlack WR, Wang W. 2000. Designing functional foods to enhance health. In: Phytochemicals as Bioactive Agents . CRC Press. Boca Raton, Florida, USA. pp. 241-270.
Chatterjee RN, Raizada MB. 1963. Culmsheaths as an aid to identification of Bamboos. The Indian Forester 89:744–756. DOI: 10.36808/if/1963/v89i11/24945.
Clark LG, Londoño X, Ruiz-Sanchez E. 2015. Bamboo taxonomy and habitat. In: Liese, W., Kohl, M. (eds) Bamboo. Tropical Forestry, Vol 10. Springer International Publishing, Cham., Switzerland. DOI: 10.1007/978-3-319-14133-6_1.
Clark L. 2005. Bamboo biodiversity: Bamboo Character Lists. Iowa State University.
DENR-ERDB. 2016. Sustaining our bamboos. Canopy International Vol 2 No. 1. Ecosystems Research and Development Bureau, Department of Environment and Natural Resources, Los Baños, Laguna, Philippines. 16p.
Deogun PN. 1937. The silviculture and management of the bamboo Dendrocalamus strictus. Indian Forest Records (n.s.) (Silviculture) 2: 75-173.
Dev SA, Sijimol K, Prathibha PS, Sreekumar VB, Muralidharan EM. 2020. DNA barcoding as a valuable molecular tool for the certification of planting materials in bamboo. 3 Biotech 10: 1-12. DOI: 10.1007/s13205-019-2018-8.
Dionglay MS, Lapuz RB, Ramos RE, Lapuz ARP, Bisana GRB. 2018. Phytochemical analysis and evaluation of the antimicrobial and antioxidant properties of Philippine bamboo during dry season. Philippine Forest Products Journal 9:1-10.
Doyle JJ, Doyle JL. 1990. Isolation of plant DNA from fresh tissue. Focus 12:13–15.
Dransfield S. 1994. Bamboo resources in Thailand: how much do we know? In: Proceedings 4th International Bamboo Workshop. IDRCFAO-UNDP. Ottawa, Canada. pp. 1-4.
Gonzales LL, Cortiguerra NG, Piñol AA. 1992. Reforestation species Kauayan kiling (Bambusa vulgaris Schrad. ex Wendl.) and Bolo (Gigantochloa levis (Blanco) Merr.). Research Information Series on Ecosystems Vol 4 No. 4. Department of Environment and Natural Resources, Ecosystems Research and Development Bureau. Los Baños, Laguna, Philippines. 11p.
Guevarra BQ. 2005. A Guidebook to Plant Screening: Phytochemical and Biological.UST Publishing House, España, Manila, Philippines.
Hall TA. 1999. BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95-98.
Hammer KA, Carson CF, Riley TV. 2003. Antifungal activity of the components of Melaleuca alternifolia (tea tree) oil. Journal of Applied Microbiology 95(4): 853-860. DOI: 10.1046/j.1365-2672.2003.02059.x.
Hostettmann KA, Marston A. 1995. Saponins: Chemistry and Pharmacology of Natural Products Vol 548. Cambridge University Press, Cambridge, United Kingdom. pp.326-327.
Hoyweghen LV, De Beer T, Deforce D, Heyerick A. 2012. Phenolic compounds and anti-oxidant capacity of twelve morphologically heterogeneous bamboo species. Phytochemical Analysis 23(5): 433-443. DOI: 10.1002/pca.1377.
Jovale VT, Tongco JVV, Remil MA, Ramon AR. 2014. Proximate analysis, phytochemical screening, and total phenolic and flavonoid content of Philippine bamboo “buho” Schizostachyum lumampao, Journal of Chemical and Pharmaceutical Research 6(1): 709-713.
Judziewicz EJ, Clark LG, Londoño X, Stern MJ. 1999. American bamboos. Smithsonian Institution, Washington, DC, USA. p. 392.
Kelchner SA, Clark LG. 1997. Molecular evolution and phylogenetic utility of the chloroplastrpl16intron in Chusquea and the Bambusoideae (Poaceae). Molecular Phylogenetics and Evolution 8(3): 385-397. DOI: 10.1006/mpev.1997.0432.
Langenheim JH. 1994. Higher plant terpenoids: a phytocentric overview of their ecological roles. Journal of Chemical Ecology 20: 1223-1280. DOI: 10.1007/BF02059809.
Melagraki G, Afantitis A, Igglessi-Markopoulou O, Detsi A, Koufaki M, Kontogiorgis C, Hadjipavlou-Litina DJ. 2009. Synthesis and evaluation of the antioxidant and anti-inflammatory activity of novel coumarin-3-aminoamides and their alpha-lipoic acid adducts. European Journal of Medicinal Chemistry 44(7): 3020-3026. DOI: 10.1016/j.ejmech.2008.12.027.
Mert-Turk F. 2006. Saponins versus plant fungal pathogens. Journal of Cell and Molecular Biology 5: 13-17.
Muñoz MNM, Alvarado UG, Reyes JIL, Watanabe K. 2021. Acute oral toxicity assessment of ethanolic extracts of Antidesma bunius (L.) Spreng fruits in mice. Toxicology Reports 8: 1289-1299. DOI: 10.1016/j.toxrep.2021.06.010.
Park EJ, Jhon DY. 2010. The antioxidant, angiotensin converting enzyme inhibition activity, and phenolic compounds of bamboo shoot extracts. LWT-Food Science and Technology 43(4): 655-659. DOI: 10.1016/j.lwt.2009.11.005.
PGC. 2022. Protocol for DNA sequencing of bamboo species. Philippine Genome Center, University of the Philippines-Diliman, Diliman, Quezon City, Metro Manila, Philippines.
Prakash D, Kumar N. 2011. Cost effective natural antioxidants. In: Gerald J, Watson R, Preedy V. (eds). Nutrients, Dietary Supplements and Nutriceuticals.Nutrition and Health Press. Springer. USA. DOI: 10.1007/978-1-60761-308-4_12.
Prasad K, Garner P, Cochrane Infectious Diseases Group. 1996. Steroids for treating cerebral malaria. Cochrane Database of Systematic Reviews 1999(3): CD000972. DOI: 10.1002/14651858.CD000972.
Price KR, Johnson IT, Fenwick GR. 1987. The chemistry and biological significance of saponins in food and feeding stuffs. Critical Reviews in Food Science and Nutrition 26: 27-135.
Quant EC, Jeste SS, Muni RH, Cape AV, Bhussar MK, Peleg AY. 2009. The benefits of steroids versus steroids plus antivirals for treatment of Bell’s palsy: a meta-analysis. BMJ 339. DOI: 10.1136/bmj.b3354.
Ricohermoso AL, Hadsall AS, Caasi-Lit MT. 2015. Morphology-based Diagnostics of edible young shoots of bamboo species (Subfamily Bambusoideae: Family Poaceae) from the Philippines. In: Proceedings of the 10th World Bamboo Congress in Damyang, South Korea.
Roxas CA. 2012. Handbook on erect bamboo species found in the Philippines. Ecosystems Research and Development Bureau, Department of Environment and Natural Resources, Los Baños, Laguna, Philippines.
Sawarkar AD, Shrimankar DD, Kumar M, Kumar P, Kumar S, Singh L. 2021. Traditional system versus DNA barcoding in identification of bamboo species: a systematic review. Molecular Biotechnology 63(8): 651-675. DOI: 10.1007/s12033-021-00337-4.
Singh M, Pal M, Sharma RP. 1999. Biological activity of the Labdane diterpenes. Planta Medica 65: 2-8.
Sinohin VO. 1990. Phenological observations on seven Philippine bamboo species. In: 2 National Bamboo Research and Development Symposium. ERDB Auditorium, College, Laguna, Philippines. 14 October 1990.
Sosa V, Mejia-Saules T, Cuéllar MA, Vovides AP. 2013. DNA barcoding in endangered Mesoamerican groups of plants. The Botanical Review 79: 469-482. DOI: 10.1007/s12229-013-9129-4.
Tholl D. 2006. Terpene synthases and the regulation, diversity and biological roles of terpene metabolism. Current Opinion in Plant Biology 9(3): 297-304. DOI: 10.1016/j.pbi.2006.03.014.
Tongco JVV, Rodriguez EB, Abasolo WP, Mun SP, Razal RA. 2016. Mineral, nutritional, and phytochemical profile, total phenolic content, and radical scavenging activity of Philippine bamboo “Bolo” Gigantochloa levis (Blanco) Merr. leaves. Natural Product Sciences 22(1): 60-63. DOI: 10.20307/nps.2016.22.1.60.
Wang AK, Lu QF, Zhu ZX, Liu SH, Zhong H, Xiao ZZ, Zou YG, Gu LJ, Du XH, Cai HF, Bi YF. 2022. Exploring phylogenetic relationships within the subgenera of Bambusa based on DNA barcodes and morphological characteristics. Scientific Reports 12(1): 1-8. DOI: 10.1038/s41598-022-12094-8.
Wani MA, Prasad S, Prakash O. 2019. Qualitative phytochemical analysis of various parts of bamboo (Bambusa balcooa) for possible therapeutic usages in bovine reproductive disorders. Journal of Pharmacognosy and Phytochemistry 8(1): 217-221.

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