Identification of bovine Eimeria species pathogenic using PCR ITS-1 in Indonesia

##plugins.themes.bootstrap3.article.sidebar##

PDF
DOI https://doi.org/10.13057/biodiv/d240850
Issue
Vol. 24 No. 8 (2023)
Section
Articles
Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

##plugins.themes.bootstrap3.article.main##

RADEN WISNU NURCAHYO ♥
Department of Parasitology, Faculty of Veterinary Medicine, Universitas Gadjah Mada. Jl. Fauna No. 2, Karanggayam, Sleman 55281, Yogyakarta, Indonesia
FITRINE EKAWASTI
Department of Parasitology, Faculty of Veterinary Medicine, Universitas Gadjah Mada. Jl. Fauna No. 2, Karanggayam, Sleman 55281, Yogyakarta, Indonesia
LINTANG WINANTYA FIRDAUSY
Department of Parasitology, Faculty of Veterinary Medicine, Universitas Gadjah Mada. Jl. Fauna No. 2, Karanggayam, Sleman 55281, Yogyakarta, Indonesia
VIKA ICHSANIA NINDITYA
Department of Parasitology, Faculty of Veterinary Medicine, Universitas Gadjah Mada. Jl. Fauna No. 2, Karanggayam, Sleman 55281, Yogyakarta, Indonesia
MUKH FAJAR NASRULLOH
Department of Parasitology, Faculty of Veterinary Medicine, Universitas Gadjah Mada. Jl. Fauna No. 2, Karanggayam, Sleman 55281, Yogyakarta, Indonesia
DYAH AYU KURNIAWATI
Indonesian Research Center for Veterinary Sciences, Indonesian Agency for Agricultural Research and Development. Jl. RE Martadinata 30, Bogor 16114, West Java, Indonesia
FATHUR ROHMAN HARYADI
Department of Parasitology, Faculty of Veterinary Medicine, Universitas Gadjah Mada. Jl. Fauna No. 2, Karanggayam, Sleman 55281, Yogyakarta, Indonesia
JOKO PRASTOWO
Department of Parasitology, Faculty of Veterinary Medicine, Universitas Gadjah Mada. Jl. Fauna No. 2, Karanggayam, Sleman 55281, Yogyakarta, Indonesia
DWI PRIYOWIDODO
Department of Parasitology, Faculty of Veterinary Medicine, Universitas Gadjah Mada. Jl. Fauna No. 2, Karanggayam, Sleman 55281, Yogyakarta, Indonesia

Abstract

Abstract. Nurcahyo RW, Ekaswasti F, Firdausy LW, Ninditya VI, Nasrulloh MF, Kurniawati DA, Haryadi FR, Prastowo J, Priyowidodo D. 2023. Identification of bovine Eimeria species pathogenic using PCR ITS-1 in Indonesia. Biodiversitas 24: 4684-4689. Bovine eimeriosis is one of Indonesia's cattle's most significant health issues caused by the intestinal protozoan parasite Eimeria spp. Eimeria spp., E. zuernii, E. alabamensis, E. aburnensis, and E. bovis are recognized as most pathogenic since they cause clinical symptoms. This study aimed to identify pathogenic Eimeria spp. and explore their evolutionary relationships using the molecular marker, the internal transcribed spacer-1 (ITS-1). A total of 171 fecal samples were collected from the provinces of Banten, Lampung, and West Java, and 43 (25.1%) were infected with oocysts of Eimeria spp. by microscopic examination. The positive samples were further confirmed for different Eimeria species by amplifying and sequencing the ITS-1 gene. After editing and alignment, the sequences were considered for analyses, and the phylogenetic analysis confirmed four species of Eimeria such as E. bovis 39 (22.8%), E. zuernii 23 (13.5%), E. alabamensis 7 (4.09%), and E. aburnensis 25 (14.6%). In this study, several Eimeria species were related to overseas species; therefore, additional risk analysis is needed to control the spread of bovine eimeriosis. It is possible to learn important details regarding the ecology and population genetics of Eimeria spp. by employing sequencing and phylogenetic analysis to categorize all positive samples of the eimeriosis as pathogenic Eimeria spp.

##plugins.themes.bootstrap3.article.details##

References
Ayana, D., Temesgen, K., Kumsa, B., Alkadir, G. 2022. Dry Season Eimeria Infection in Dairy Cattle and Sheep in and Around Adama and Bishoftu Towns, Oromia, Ethiopia. Vet Med (Auckl) 13(1): 235 – 245. DOI: 10.2147/VMRR.S377017.
Bangoura, B., Mundt, H.C., Schmäschke, R., Westphal, B., Daugschies, A. 2012. Prevalence of Eimeria bovis and Eimeria zuernii in German cattle herds and factors influencing oocyst excretion. Parasitol Res 110(2): 875 – 881. DOI: 10.1007/s00436-011-2569-z.
Bruhn, F.R., Lopes, M.A., Demeu, F.A., Perazza, C.A., Pedrosa, M.F., Guimarães, A.M. 2011. Frequency of species of Eimeria in females of the Holstein-Friesian breed at the post-weaning stage during autumn and winter. Rev Bras Parasitol Vet 20(4): 303 – 307. DOI: 10.1590/s1984-29612011000400008.
Cardim, S.T., Seixas, M., Tabacow, V.B.D., Taroda, A., Carneiro, P.G., Martins, T.A., de Barros, L.D., Minutti, A.F., Chryssafidis, A.L., Vidotto, O., Garcia, J.L. 2018. Prevalence of Eimeria spp. in calves from dairy farms in northern Paraná state, Bazil. Braz J Vet Parasitol 27(1): 118 – 122. DOI: 10.1590/S1984-29612017072.
Daughschies, A., and Najdrowski, M. 2005. Eimeriosis in cattle: current understanding. J Vet Med 52(1): 417 – 427. DOI: 10.1111/j.1439-0450.2005.00894.x.
Deb, L.C., Agmed, S.S.U.A., Baidhya, C.C., Nath, N.D., Chosh, S., Paul, S. 2022. Prevalence of Eimeria spp. with associated risk factors in dairy calves in Sylhet, Bangladesh. Vet Med Sci 8(1): 1250 – 1257. DOI: 10.1002/vms3.776.
Dong, H., Zhao, Q., Han, H., Jiang, L., Zhu S, Li T, Kong C, Huang B. 2012. Prevalence of coccidial infection in dairy cattle in Shanghai, China. J Parasitol 98(5): 963 – 966. DOI: 10.1645/GE-2966.1.
Ekawasti, F., Nurcahyo, R.W., Firdausy, L.W., Wardhana, A.H., Sawitri, D.H., Prastowo, J., Priyowidodo, D. 2021. Prevalence and risk factors associated with Eimeria species infection in cattle of different geographical regions of Indonesia. Vet World 14(9): 2339 – 2345. DOI: 10.14202/vetworld.2021.2339-2345.
Ekawasti, F., Nurcahyo, R.W., Nashrulloh, M.F., Priyowidodo, D., Prastowo. J. 2022. Development of a multiplex polymerase chain reaction technique for detection and discrimination of Eimeria spp. in cattle in Indonesia, Vet World 15(4): 975 – 980. DOI: 10.14202/vetworld.2022.975-980.
Ekawasti, F., Nurcahyo, W., Wardhana, A.H., Shibahara, T., Tokoro, M., Sasai, K., Matsubayashi, M. 2019. Molecular characterization of highly pathogenic Eimeria species among beef cattle on Java Island, Indonesia. Parasitol Int 72: 101927. DOI: 10.1016/j.parint.2019.101927.
Ekawasti, F., and Wardhana, A.H. 2019. Coccidiosis disease in cattle in Indonesia and development of diagnostic techniques. J Wartazoa 29(3): 133-144. DOI: http://dx.doi.org/10.14334/wartazoa.v29i3.2010.
Enemark, H.L., Dahl, J., Enemark, J.M.D. 2013. Eimeriosis in Danish dairy calves – correlation between species, oocyst excretion and diarrhoea. Parasitol Res 112(1): 169 – 176. DOI: 10.1007/s00436-013-3441-0.
Hamid, P.H., Kristianingrum, Y.P., Prastowo, J., da Silva, L.M.R. 2016. Gastrointestinal parasites of cattle in Central Java. Am J Anim Vet Sci 11: 119–124. DOI: https://doi.org/10.3844/ajavsp.2016.119.124.
Hastutiek, P., Lastuti, N.D.R, Suwanti, L.T., Sunarso, A., Kurniawati, D.A., Yudhana, A. 2022. Occurrence and biodiversity of Eimeria spp. (Apicomplexa: Eimeriidae) in Madura cattle reared on Kamal Subdistrict, Madura Island, Indonesia. Vet World 15(8): 2084 – 2088. DOI: 10.14202/vetworld.2022.2084-2088.
Keeton, S.T.N., and Navarre, C.B. 2018. Coccidiosis in large and small ruminants. Vet Clin North Am Food Anim Pract 34: 201 – 208. DOI: 10.1016/j.cvfa.2017.10.009.
Kim, H.C., Choe, C., Kim, S.H., Chae, J.S., Yu, D.H., Park, J., Park, B.K., Choi, K.S. 2018. Epidemiological survey on Eimeria spp. associated with diarrhea in pre-weaned native Korean calves. Korean J Parasitol 56(6): 619 – 623. DOI: 10.3347/kjp.2018.56.6.619.
Koutny, H., Joachi, A., Tichy, A., Baumgartner, W. 2012. Bovine Eimeria species in Austria. Parasitol Res 110: 1893–1901. DOI: 10.1007/s00436-011-2715-7.
Lasen, B., and Jarvis, T. 2009. Eimeria and Cryptosporidium in Lithuanian cattle farms. Vet Med Zoot 48(70): 24 – 28.
Li, D.L., Gong, Q.L., Ge, G.Y., Wang, Q., Sheng, C.Y., Ma, B.Y., Chen, Z.Y., Yang, Y., Li, J.M., Shi, K., Leng, X., Du, R. 2021. Prevalence and infection risk factors of bovine Eimeria in China: a systematic review and meta-analysis. Parasite 28: 61. DOI: 10.1051/parasite/2021055
Loo, S., Lim, L., Efendi, N., Blake, D.P., Kawazu, S., Wan, K. 2019. Comparison of Molecular Methods for the Detection of Eimeria in Domestic Chickens in Malaysia. Sains Malaysiana 48(7): 1425-1432. DOI:10.17576/jsm-2019-4807-11.
Malek, S.S., and Kuraa, H.M. 2018. Detection and identification of Eimeria species in Naturally Infected Calves at Assiut Goveernote. Zagazig Vet J 46(1): 60 – 69. DOI: 10.21608/ZVJZ.2018.7624.
Matjila, P.T., and Penzhorn, B.L. 2002. Occurrence and diversity of bovine coccidia at three localities in South Africa. Vet Parasitol 104(2): 93 – 102. DOI: 10.1016/s0304-4017(01)00605-7.
[MoARI] Ministry of Agriculture Republic Indonesia. 2018. Livestock and Animal Health Statistic. Indonesia.
Morgoglione, M.E., Bosco, A., Maurelli, M.P., Alves, L.C., Saralli, G., Bruni, G., Cringoli, G., Rinaldi, L. 2020. A 10-year Surveillance of Eimeria spp. in cattle and buffaloes in a Mediterranean Area. Front Vet Sci, 7: 410. DOI: 10.3389/fvets.2020.00410.
Sánchez, R.O., Romero, J.R., Founroge, R.D. 2008. Dynamics of Eimeria oocyst excretion in dairy calves in the Province of Buenos Aires (Argentina), during their first 2 months of age. Vet Parasitol 151: 133 – 188. DOI: 10.1016/j.vetpar.2007.11.003.
Sufi, I.M., Cahyaningsih, U., Sudarnika, E. 2017. Eimeria species composition and factors influencing oocysts shedding in dairy farm, Bandung, Indonesia. Biotropika 24(2): 104 – 113. DOI: https://doi.org/10.11598/btb.2017.24.2.516.
Taylor, M.A., Coop, R.L., Wall, R.L. 2016. Veterinary Parasitology, 4th ed. John Wiley & Sons Ltd., Oxford, UK.
Zefanya, F., Apsari, I., and Oka. I. Prevalensi dan Identifikasi Protozoa Eimeria sp., dan Isospora sp., Intestinal Sapi Bali di Dataran Tinggi dan Dataran Rendah Basah. Indones Med Veterinus 10(2): 180 – 188. DOI: 10.19087/imv.2021.10.2.180.

Most read articles by the same author(s)

<< < 1 2