Diversity and spatial distribution of Order Diptera from Kuala Keniam National Park, Pahang, Malaysia




Abstract. Amin IN, Fathiah KN, ‘Aliyaa NN, Badrina MNN, Nadiah MYN, Hasnah KS, Norhafizah MZ, Khairiyah MHS. 2023. Diversity and spatial distribution of Order Diptera from Kuala Keniam National Park, Pahang, Malaysia. Biodiversitas 24: 4668-4674. A study on the diversity and distribution of order Diptera was conducted in different Kuala Keniam National Park, Malaysia landscapes. Three malaise traps were placed in three landscapes: the forest fringe, middle forest, and inner forest. A total of 1920 Diptera were recorded from 19 families and 109 morphospecies of order Diptera. Anthomyiidae, Calliphoridae, Pipunculidae, Scenopinidae, Ulidiidae, Tephritidae, Ceratopogonidae, Stratiomyidae, Asilidae, Syrphidae, Tipulidae, Ephydridae, Cecidomyiidae, Dolichopodidae, Phoridae, Culicidae, Muscidae, Sciaridae, and Drosophilidae were the identified families. The family with the highest number of individuals collected was Phoridae, with 89 individuals (5 morphospecies) collected from the forest fringe, followed by 271 individuals (6 morphospecies) from the middle forest and 697 individuals from the inner forest (7 morphospecies). Pipunculidae had the least collected individuals, with only one in the inner forest. The forest fringe recorded the highest Shannon-Wiener Diversity Index (2.13), followed by the middle forest (1.68) and the inner forest (1.04). The forest fringe also recorded the highest Evenness Index and Margalef Richness Index with 0.44 and 3.16, respectively. The distribution of Diptera differed significantly along the environment gradients from the forest fringe to the inner forest (p<0.05), and the pairwise comparison showed that the difference comes from Trap 2 and Trap 3 (p<0.001). The forest fringe was more diversified than the middle and the inner forest, even though the number of individuals was low. Vegetation types and sunlight might be the reasons behind this discovery. The results from this study can be used as baseline data for future reference on the diversity of Diptera from the forest reserve.


Abdul Hamid, S., Md Rawi, C. S., & Huda, N. (2014). Seasonal abundance and diversity of aquatic insects in rivers in Gunung Jerai Forest Reserve, Malaysia. Sains Malaysiana, 43(5), 667-674.
Asari N. & Suratman, M. N. (2010). Distribution and species diversity of bamboo at Kuala Keniam, Pahang National Park. 2010 International Conference on Science and Social Research (CSSR 2010), 1019-1023. https://doi.org/10.1109/cssr.2010.5773680
Banafshi, O., Abai, M., Ladonni, H., Bakshi, H., Karami, H., & Hamidian, S. (2013). The fauna and ecology of mosquito larvae (Diptera: Culicidae) in western Iran. Turkish Journal Of Zoology. https://doi.org/10.3906/zoo-1206-12
Brown, B. (2001). Flies, Gnats, and Mosquitoes. Encyclopedia of Biodiversity, 488-496. https://doi.org/10.1016/b978-0-12-384719-5.00167-2
Calcaterra, L., Porter, S., & Briano, J. (2005). Distribution and Abundance of Fire Ant Decapitating Flies (Diptera: Phoridae: <I>Pseudacteon</I>) in Three Regions of Southern South America. Annals Of The Entomological Society Of America, 98(1), 85-95. https://doi.org/10.1603/0013-8746(2005)098[0085:daaofa]2.0.co;2
Campbell, N. A., Reece, J. B., Urry, L. A., Cain, M. L., Alexander, S., Minorsky, P. V. & Jackson, R. B., Campbell, N. A. (2014). Campbell biology: a global approach (10th ed.). Pearson. 1351 pp.
Cardoso, P., Barton, P. S., Birkhofer, K., Chichorro, F., Deacon, C., Fartmann, T., Fukushima, C. S., Gaigher, R., Habel, J. C., Hallmann, C. A., Hill, M. J., Hochkirch, A., Kwak, M. L., Mammola, S., Ari Noriega, J., Orfinger, A. B., Pedraza, F., Pryke, J. S., Roque, F. O. & Settele, J. (2020). Scientists’ warning to humanity on insect extinctions. Biological Conservation, 242(108426). https://doi.org/10.1016/j.biocon.2020.108426
Cicero, J., Adair, M., Adair, R., Hunter, W., Avery, P., & Mizell, R. (2017). Predatory Behavior of Long-Legged Flies (Diptera: Dolichopodidae) and Their Potential Negative Effects on the Parasitoid Biological Control Agent of the Asian Citrus Psyllid (Hemiptera: Liviidae). Florida Entomologist, 100(2), 485-487. https://doi.org/10.1653/024.100.0243
Coetzee, M. (2014). How important are Dipteran vectors of disease in Africa? Transactions of the Royal Society of Tropical Medicine and Hygiene, 108(4), 179–180. https://doi.org/10.1093/trstmh/tru020
Courtney, G. W. & Merritt, R. W. (2009). Diptera (Non-Biting Flies). Encyclopedia of Inland Waters, 288–298. https://doi.org/10.1016/b978-012370626-3.00170-8
Courtney, G. W., Pape, T., Skevington, J. H. & Sinclair, B. J. (2017). Biodiversity of Diptera. Insect biodiversity: science and society, 1, 229-278.
Cranston, P. S. & Gullan, P. J. (2009). Phylogeny of Insects. Encyclopedia of Insects, 780–793. https://doi.org/10.1016/b978-0-12-374144-8.00208-3
Foottit, R. G. & Adler, P. H. (2017). Insect Biodiversity: Science and Society (2nd ed., Vol. 1). John Wiley & Sons. 912 pp.
Gerhardt, R., & Hribar, L. (2019). Flies (Diptera). Medical And Veterinary Entomology, 171-190. https://doi.org/10.1016/b978-0-12-814043-7.00011-x
Gibb, T. (2015). Pest Insects. Contemporary Insect Diagnostics, 153-245. https://doi.org/10.1016/b978-0-12-404623-8.00005-3
Goulson, D. (2019). The insect apocalypse, and why it matters. Current Biology, 29(19), 967–971. https://doi.org/10.1016/j.cub.2019.06.069
Grimaldi, D. & Engel, M. (2006). Evolution of the insects. Cambridge University Press. 772 pp.
Hall, R., & Gerhardt, R. (2002). FLIES ( Diptera ). Medical And Veterinary Entomology, 127-145. doi: 10.1016/b978-012510451-7/50010-4
Hamid, N. H. A. & Suratman, M. N. (2010, December). Assessment of rattan abundance and species composition at Kuala Keniam, Taman Negara Pahang. In 2010 International Conference on Science and Social Research (CSSR 2010). 1041-1046.
Hartley, D. (1992). Poinsettias. Introduction To Floriculture, 305-331. https://doi.org/10.1016/b978-0-12-437651-9.50017-8
Hayati, M. D., Noryati A., & Ho, C. S. F. (2010). Crisis Management at Taman Negara National Park, Kuala Tahan, Pahang, Malaysia. International Journal of Trade, Economics and Finance, 1(1), 24–27. https://doi.org/10.7763/ijtef.2010.v1.4
Ibrahim, Y. & Hassan, M. S. (2011). Tourism management at Taman Negara (national park), Pahang, Malaysia: conflict and synergy. Journal of Ritsumeikan Social Sciences and Humanities, 3, 109–122.
Kautz, A., & Gardiner, M. (2019). Agricultural intensification may create an attractive sink for Dolichopodidae, a ubiquitous but understudied predatory fly family. Journal Of Insect Conservation, 23(3), 453-465. https://doi.org/10.1007/s10841-018-0116-2
Kechev, M. (2021). Data on the distribution of Dolichopodidae (Diptera: Empidoidea) in Bulgaria, with first records for the country. Historia Naturalis Bulgarica, 42(13), 95-102. https://doi.org/10.48027/hnb.42.131
Kocatas, A. and Bilecik, N. (1992). Aegean Sea and Its Living Resources. T. C. Tarimve Köyisleri Bakanligi. Su Ürünleri Arastirma Enstitüsü Müdürlügü. Bodrum.Seri A. 7: 88 pp.
Merritt, R. W., Courtney, G. W. & Keiper, J. B. (2009). Diptera. Encyclopedia of Insects, 284–297. https://doi.org/10.1016/b978-0-12-374144-8.00085-0
Mwakalukwa, E., Meilby, H., & Treue, T. (2014). Floristic Composition, Structure, and Species Associations of Dry Miombo Woodland in Tanzania. ISRN Biodiversity, 2014, 1–15. https://doi.org/10.1155/2014/153278
Namaki-Khameneh, R., Khaghaninia, S., L. Disney, R., & Maleki-Ravasan, N. (2021). The scuttle flies (Diptera: Phoridae) of Iran with the description of Mahabadphora aesthesphora as a new genus and species. PLOS ONE, 16(10), e0257899. https://doi.org/10.1371/journal.pone.0257899
Price, P. W., Denno, R. F., Eubanks, M. D., Finke, D. L. & Kaplan, I. (2011). Insect ecology: behavior, populations and communities. Cambridge University Press. 828 pp.
Resh, V. H. & Cardé, R. T. (2003). Encyclopedia of Insects (1st ed.). Academic Press. 1266 pp.
Rocha-Ortega, M., Rodriguez, P., & Córdoba-Aguilar, A. (2021). Geographical, temporal and taxonomic biases in insect GBIF data on biodiversity and extinction. Ecological Entomology, 46(4), 718–728. https://doi.org/10.1111/een.13027
Rooke, R., Rasool, A., Schneider, J., & Levine, J. D. (2020). Drosophila melanogaster behaviour changes in different social environments based on group size and density. Communications Biology, 3(1). https://doi.org/10.1038/s42003-020-1024-z
Sarwar, M. (2020). Life cycle and development of diptera. Intechopen. 182pp.
Skendži?, S., Zovko, M., Živkovi?, I. P., Lesic, V., & Lemi?, D. (2021). The Impact of Climate Change on Agricultural Insect Pests. Insects, 12(5), 440. https://doi.org/10.3390/insects12050440
Smith, B. D., & Wilson, J. R. (1996). A Consumer’s Guide to Evenness Indices. Oikos, 76(1), 70. https://doi.org/10.2307/3545749
Srisuka, W., Sulin, C., Aupalee, K., Phankaen, T., Taai, K., & Thongsahuan, S. et al. (2021). Community Structure, Biodiversity and Spatiotemporal Distribution of the Black Flies (Diptera: Simuliidae) Using Malaise Traps on the Highest Mountain in Thailand. Insects, 12(6), 504. https://doi.org/10.3390/insects12060504
Steinke, D., Braukmann, T. W., Manerus, L., Woodhouse, A. & Elbrecht, V. (2021). Effects of Malaise trap spacing on species richness and composition of terrestrial arthropod bulk samples. Metabarcoding and Metagenomics, 5, 43-50. https://doi.org/10.3897/mbmg.5.59201
Suratman, M. N. (2012). Tree Species Diversity and Forest Stand Structure of Pahang National Park, Malaysia. Biodiversity Enrichment in a Diverse World, 19, 45-46. https://doi.org/10.5772/50339
Suratman, M. N., Kusin M., Shaikh Abdul Karim Yamani Zakaria, Saleh K., Ahmad M. & Bahari S. A. (2010). Stand structure and species diversity of Keniam forest, Pahang National Park. 2010 International Conference on Science and Social Research (CSSR 2010), 766–771. https://doi.org/10.1109/cssr.2010.5773887
UNESCO World Heritage Centre. (2014, June 5). National Park (Taman Negara) of Peninsular Malaysia - UNESCO World Heritage Centre. UNESCO. Retrieved December 14, 2021, from https://whc.unesco.org/en/tentativelists/5927/
Wajnberg, E. & Colazza, S. (2013). Chemical ecology of insect parasitoids. John Wiley & Sons Inc. 328 pp.
Wang, Y., Zhang, Y., Hu, G., Wang, M., Zhu, R., & Zhai, Y. et al. (2020). Development of Megaselia spiracularis (Diptera: Phoridae) at different constant temperatures. Journal Of Thermal Biology, 93, 102722. https://doi.org/10.1016/j.jtherbio.2020.102722
Zani, N. F. & Suratman, M. N. (2011). Estimation of above ground biomass of Keniam forests, Taman Negara Pahang. 2011 IEEE Symposium on Business, Engineering and Industrial Applications (ISBEIA), 80-83. https://doi.org/10.1109/isbeia.2011.6088891