Effectiveness of Rhodococcus erythropolis strain OPI-01 on the fungal development in winter wheat




Abstract. Behzad A, Diakite S, Astarkhanova TS, Pakina EN, Allen D, Mohammadi PM, Diarra O, Saquee FS. 2024. Effectiveness of Rhodococcus erythropolis strain OPI-01 on the fungal development in winter wheat. Biodiversitas 25: 1063-1070. One of the means of sustainable wheat production is using bacterial pesticides, which can synthesize phytohormonal substances with antibacterial and antifungal action. Therefore, the work aimed to study the effect of different doses of a new growth regulator Restart, ZH (Rhodococcus erythropolis D strain OPI-01) on winter wheat yield. The findings of field experiments on the effect of plant growth regulator R. erythropolis strain OPI-01 method of pre-sowing treatment of seeds (at 0.1 L/t and 0.2 L/t), and soil spraying just before sowing (-0.5 l/ha) revealed that on average, during the 2020-2021 and 2021-2022 seasons, the number of productive stems increased by a margin of 23-30, the number of grains in 1 ear increased in the experimental units by 16.2-19.4%, and the length of the ear by 0.3-0.7cm, gluten varied from 32.3 to 33.8%, yield increased from 27.8-33.0% relative to the control. Disease severity and incidence of powdery mildew, brown rust and Septoria disease in plots amended withR. erythropolis strain OPI-01 was 2-fold less than in the control plots. Research trials show the efficacy of R. erythropolis strain OPI-01 under field conditions as a plant growth regulator and a potential microbial fungicide against some foliar diseases of winter wheat. It would be exciting to study the mechanism of R. erythropolis on wheat pathogens in vitro and in planta which are still less developed in the literature.


Aseeva T A, Zenkina K V, Trifuntova I B, et al. 2020. Fungal diseases on cereals in the monsoon climate of the Russian Far East. Dostizheniya nauki i tekhniki APK 34(12): 12-8. DOI: 10.24411/0235-2451-2020-11202 (in Russian)
Barbey C, Crépin A, Bergeau D, Ouchiha A, Mijouin L, Taupin, L, Orange N, Feuilloley M, Dufour A, Burini J F, & Latour X. 2013. In planta biocontrol of Pectobacterium atrosepticum by Rhodococcus erythropolis involves silencing of pathogen communication by the rhodococcal gamma-lactone catabolic pathway. PloS one 8(6): e66642. DOI: 10.1371/journal.pone.0066642
Batakova OB, Korelina VA. 2017. The effect of yield structure elements on spring barley (Hordeum vulgare L.) productivity in the environments of Russia’s extreme north. Proceedings on applied botany, genetics and breeding 178(3): 50-58. DOI: 10.30901/2227-8834-2017-3-50-58 (In Russian)
Chane A, Barbey C, Robert M, Merieau A, Konto-Ghiorghi Y, Beury-Cirou A, & Latour X. 2019. Biocontrol of soft rot: confocal microscopy highlights virulent pectobacterial communication and its jamming by Rhodococcal quorum-quenching. Molecular PlantMicrobe Interactions 32(7): 802-812. DOI: 10.1094/MPMI-11-18-0314-R
Cserháti M, Kriszt B, Krifaton C, Szoboszlay S, Háhn J, Tóth S, Nagy I, & Kukolya J. 2013. Mycotoxin-degradation profile of Rhodococcus strains. International journal of food microbiology 166(1): 176–185. DOI: 10.1016/j.ijfoodmicro.2013.06.002
Feng ZH, Wang LY, Yang ZQ, Zhang YY, Li X, Song L, He L, Duan JZ and Feng W. 2022. Hyperspectral monitoring of powdery mildew disease severity in wheat based on machine learning. Front. Plant Sc 13: 828454. DOI: 10.3389/fpls.2022.828454
Figueroa M, Hammond-Kosack K E, & Solomon P S (2018) A review of wheat diseases-a field perspective. Molecular plant pathology 19: 1523–1536. DOI: 10.1111/mpp.12618
Isaychev V, Andreev N, & Bogapova M. 2020. The influence of growth regulators on the productive capacity of spring wheat. In BIO Web of Conferences, EDP Sciences 17: 00106. DOI: 10.1051/bioconf/20201700106
Kang K, Niu Z, Zhang W, Wei S, Lv Y, Hu Y. 2023. Antagonistic Strain Bacillus halotolerans Jk-25 mediates the biocontrol of wheat common root rot caused by Bipolaris sorokiniana. Plants 12: 828. DOI: 10.3390/plants12040828
Kim D, Choi KY, Yoo M, Zylstra GJ, Kim E. 2018. Biotechnological potential of Rhodococcus biodegradative pathways. J. Microbiol. Biotechnol 28: 1037- 1051. DOI: 10.4014/jmb.1712.12017
Kekalo AYu. 2021. An eco-friendly way to protect wheat seeds from phytopathogens. Agrarian science. 354(11-12): 129-133. DOI: 10.32634/0869- 8155-2021-354-11-12-129-133 (In Russian)
Kuhl T, Chowdhury SP, Uhl J, Rothballer M. 2021.Genome-Based Characterization of Plant-Associated Rhodococcus qingshengii RL1 reveals stress tolerance and plant microbe interaction traits. Frontiers in microbiology 12: 708605. DOI: 10.3389/fmicb.2021.708605
Lammas M E, Shitikova AV .2021. Influence of biostimulants of growth on germination energy, germination and intensity of germination of spring barley seeds. Plodorodie 5 (122): 61–64. DOI: 10.25680/S19948603.2021.122.15 (In Russian)
Latour X, Barbey C, Chane A, Groboillot A, Burini JF. 2013. Rhodococcus erythropolis and Its ?-lactone catabolic pathway: an unusual biocontrol system that disrupts pathogen quorum sensing communication. Agronomy 3: 816-838. DOI: 10.3390/agronomy3040816
Mavrodi DV, Mavrodi OV, Elbourne LDH, Tetu S, Bonsall RF, Parejko J, Yang M, Paulsen IT, Weller DM and Thomashow LS. 2018. Long-term irrigation affects the dynamics and activity of the wheat rhizosphere microbiome. Front. Plant Sci 9: 345. DOI: 10.3389/fpls.2018.00345
McLeod MP, Warren RL, Hsiao WW, et al. 2006. The complete genome of Rhodococcus sp. RHA1 provides insights into a catabolic powerhouse. Proceedings of the National Academy of Sciences of the United States of America 103(42): 15582–15587. DOI: 10.1073/pnas.0607048103
Mulk S, Wahab A, Yasmin H, Mumtaz S, El-Serehy HA, Khan N and Hassan MN. 2022. Prevalence of wheat associated Bacillus spp. and their bio-control efficacy against Fusarium root rot. Front. Microbiol 12: 798619. DOI: 10.3389/fmicb.2021.798619
Shitikova A V, Lammas ME. 2022. Sowing qualities of spring barley seeds when using biostimulants. In IOP Conference Series: Earth and Environmental Science, IOP Publishing1112 (1): 012091. DOI: 10.1088/1755-1315/1112/1/012091
Rizvi A, Zaidi A, Ameen F, Ahmed B, AlKahtani M D, & Khan MS. 2020. Heavy metal induced stress on wheat: phytotoxicity and microbiological management. RSC advances 10(63):38379-38403. DOI: 10.1039/D0RA05610C
Rosenkranz T, Kidd P, Puschenreiter M. 2018. Effect of bacterial inoculants on phytomining of metals from waste incineration bottom ash. Waste Management 73: 351-359. DOI: 10.1016/j.wasman.2017.12.006
Sanin SS. 2016. Phytosanitary examination of the grain field and decision-making on the protective spraying of wheat with fungicides. Plant Protection and Quarantin 5:54-88 (in Russian)
Saquee FS, Norman PE, Saffa MD, Kavhiza NJ, Pakina E, Zargar M, Diakite S, Stybayev G, Baitelenova A, and Kipshakbayeva G. 2023. Impact of different types of green manure on pests and disease incidence and severity as well as growth and yield parameters of maize. Heliyon 9(6): e17294. DOI: 10.1016/j.heliyon.2023.e17294
Tan J, De Zutter N, De Saeger S, De Boevre M, Tran TM, van der Lee T, Waalwijk C, Willems A, Vandamme P, Ameye M and Audenaert K. 2021. Presence of the weakly pathogenic Fusarium poae in the Fusarium head blight disease complex hampers biocontrol and chemical control of the virulent Fusarium graminearum pathogen. Front. Plant Sci 12: 641890. DOI: 10.3389/fpls.2021.641890
Trivedi P, Pandey A, & Sa T. 2007. Chromate reducing and plant growth promoting activities of psychrotrophic Rhodococcus erythropolis MtCC 7,905. Journal of basic microbiology 47(6): 513–517. DOI: 10.1002/jobm.200700224
UNEP. 2021. Environmental and health impacts of pesticides and fertilizers and ways of minimizing them (R). https://www.unep.org/resources/report/environmental-and-healthimpacts-pesticides-and-fertilizers-and-ways-minimizing. accessed 20 January 2023.
Yin C, Hagerty CH, and Paulitz TC. 2022. Synthetic microbial consortia derived from rhizosphere soil protect wheat against a soilborne fungal pathogen. Front. Microbiol 13: 908981. DOI: 10.3389/fmicb.2022.908981