Physicochemical and microbiological properties of fermented milk using lactic acid bacteria isolated from dangke

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SETIAWAN PUTRA SYAH
ANDI NURUL MUKHLISAH
WENY DWI NINGTIYAS
MUHAMMAD IRFAN
NASRIA ANANDA
ANNISA AMALIA
TASMIN

Abstract

Abstract. Syah SP, Mukhlisah AN, Ningtiyas WD, Irfan M, Ananda N, Amalia A, Tasmin. 2024. Physicochemical and microbiological properties of fermented milk using lactic acid bacteria isolated from dangke. Biodiversitas 25: 3876-3883. This study aimed to investigate the effect of milk and starter culture type (commercial and lactic acid bacteria (LAB) isolated from dangke). Two strains of LAB (Lactobacillus fermentum A323L and B111K) and commercial culture were tested for physicochemical and microbiological properties in milk fermentation. The results showed that the type of culture had a significant effect (p<0.05) on pH, titratable acidity, WHC, syneresis, viscosity, and total number of LAB, while the type of milk had a significant effect (p<0.05) on almost all variables, except for protein content and L* (lightness) of fermented milk. Two strains of LAB isolate from dangke produce pH values, titratable acidity, and viscosity that were relatively good compared to commercial cultures and even tended to be better, specifically in terms of the total number of LAB from fermented milk produced. Skimmed milk produced the best-fermented milk in terms of composition, color, pH value, acidity, and viscosity, but was not very good as a medium for LAB growth due to the low number of microbes compared to the other two types, specifically UHT (Ultra High Temperature) low-fat. However, the number of LAB in skimmed milk met the quality standards for fermented milk products. In conclusion, both strains of LAB isolated from dangke could be used as starter cultures in the manufacture of fermented milk with good physicochemical and microbiological properties.

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References
Aini M, Rahayuni S, Mardina V, Quranayati Q, Asiah N. 2021. Bakteri Lactobacillus spp. dan peranannya bagi kehidupan. Jurnal Jeumpa 8 (2): 614 -624. DOI: 10.33059/jj.v8i2.3154. [Indonesian]
Aljewicz M, Cichosz G, Nalepa B, Bielecka M. 2016. The effect of milk fat substitution with palm fat on lactic acid bacteria counts in cheese-like products. LWT Food Sci Technol 66: 348-354. DOI: 10.1016/j.lwt.2015.10.042.
Alsubhi M, Blake M, Nguyen T, Majmudar I, Moodie M, Ananthapavan J. 2023. Consumer willingness to pay for healthier food products: A systematic review. Obes Rev 24 (1): 1 -15. DOI: 10.1111/obr.13525.
AOAC [Association of Official Analytical Chemists]. 2010. Official methods of analysis of AOAC International. AOAC International, Gaithersburg (Maryland).
Arab M, Yousefi M, Khanniri E, Azzari M, Ghasemzadeh-Mohammadi V, Mollakhalili-Meybodi N. 2023. A comprehensive review on yogurt syneresis: Effect of processing conditions and added additives. J Food Sci Technol 60 (6): 1656 -1665. DOI: 10.1007/s13197-022-05403-6.
BSN [Badan Standardisasi Nasional]. 2009. Yoghurt. http://sispk.bsn.go.id/ SNI/DetailSNI/8111. [Indonesian]
Brodziak A, Król J, Bar?owska J, Teter A, Florek M. 2020. Changes in the physicochemical parameters of yoghurts with added whey protein in relation to the atarter bacteria strains and storage time. Animals 10 (8). DOI: 10.3390/ani10081350.
Chakraborty P, Singh T, Shivhare US, Basu S. 2021. Understanding the effect of milk composition and milking season on quality characteristics of chhana. J Texture Stud 52 (1): 45-56. DOI: 10.1111/jtxs.12558.
Chen H, Wu J, Huang R, Zhang W, He W, Deng Z, Han Y, Xiao B, Luo H, Qu W. 2022. Effects of temperature and total solid content on biohydrogen production from dark fermentation of rice straw: Performance and microbial community characteristics. Chemosphere 286 (P1): 131655. DOI: 10.1016/j.chemosphere.2021.131655.
Dai S, Corke H, Shah NP. 2016. Utilization of konjac glucomannan as a fat replacer in low-fat and skimmed yogurt. J Dairy Sci 99 (9): 7063- 7074. DOI: 10.3168/jds.2016-11131.
de Souza EL, de Oliveira KÁ, de Oliveira ME. 2023. Influence of lactic acid bacteria metabolites on physical and chemical food properties. Curr Opin Food Sci 49: 100981. DOI: 10.1016/j.cofs.2022.100981.
de Souza WFC, Souza do Amaral CR, Lima da Silva BPD. 2021. The addition of skimmed milk powder and dairy cream influences the physicochemical properties and the sensory acceptance of concentrated Greek-style yogurt. Intl J gastron Food Sci 24: 100349. DOI: 10.1016/j.ijgfs.2021.100349.
FAO-WHO Committee. 2012. Codex alimentarius commission: Codex standard for fermented milks. https://www.fao.org/fao-who-codexalimentarius/en/.
Fatmarani R, Arief II, Budiman C. 2018. Purification of bacteriocin from Lactobacillus plantarum IIA-1A5 grown in various whey cheese media under freeze dried condition. Trop Anim Sci J 41 (1): 53 -59. DOI: 10.5398/tasj.2018.41.1.53.
Ge Z, Bao X, Li Z, Chen X, Li W, Rui X, Wu J, Zhang Q, Dong M. 2022. In situ exopolysaccharides produced by Lactobacillus helveticus MB2-1 and its effect on gel properties of Sayram ketteki yoghurt. Intl J Biol Macromol 208: 314-323. DOI: 10.1016/j.ijbiomac.2022.03.027.
Hadjimbei E, Botsaris G, Chrysostomou S. 2022. Beneficial effects of yoghurts and probiotic fermented milks and their functional food potential. Foods 11 (17): 2691. DOI: 10.3390/foods11172691.
Icer MA, Özbay S, A?agündüz D, Kelle B, Bartkiene E, Rocha JMF, Ozogul F. 2023. The impacts of Acidophilic lactic acid bacteria on food and human health: A review of the current knowledge. Foods 12 (15): 2965. DOI: 10.3390/foods12152965.
Jensen BAH, Heyndrickx M, Jonkers D et al. 2023. Small intestine vs. colon ecology and physiology: Why it matters in probiotic administration. Cell Reports Med 4 (9): 101190. DOI: 10.1016/j.xcrm.2023.101190.
Joshi TJ, Salini SV, Mohan L, Nandagopal P, Arakal JJ. 2024. Functional metabolites of probiotic lactic acid bacteria in fermented dairy products. Food Human 3: 100341. DOI: 10.1016/j.foohum.2024.100341.
Kalhoro MS, Anal AK, Kalhoro DH, Hussain T, Murtaza G, Mangi MH. 2023. Antimicrobial sctivities and biopreservation potential of lactic acid bacteria (LAB) from raw buffalo (Bubalus bubalis) milk. Oxid Med Cell Longev 2023: 8475995. DOI: 10.1155/2023/8475995.
Kew B, Holmes M, Stieger M, Sarkar A. 2020. Review on fat replacement using protein-based microparticulated powders or microgels: A textural perspective. Tren Food Sci Technol 106: 457-468. DOI: 10.1016/j.tifs.2020.10.032.
Kim HJ, Youn HY, Moon JS, Kim H, Seo KH. 2024. Comparative anti-microbial and anti-biofilm activities of postbiotics derived from kefir and normal raw milk lactic acid bacteria against bovine mastitis pathogens. Lwt 191: 115699. DOI: 10.1016/j.lwt.2023.115699.
Legarová V, Kou?imská L. 2010. Sensory quality evaluation of whey-based beverages. Mljekarstvo 60 (4): 280-287.
Lewis JA, Fleming JT. 1995. Chapter 1 basic culture methods. Method Cell Biol 48: 3-29. DOI: 10.1016/S0091-679X(08)61381-3.
Li N, Yang M, Guo Y, Tong LT, Wang Y, Zhang S, Wang L, Fan B, Wang F, Liu L. 2022. Physicochemical properties of different pea proteins in relation to their gelation ability to form lactic acid bacteria induced yogurt gel. Lwt 161: 113381. DOI: 10.1016/j.lwt.2022.113381.
Lin YTJ, Chou CC, Hsu CYS. 2017. Effects of Lactobacillus casei Shirota intake on caries risk in children. J Dent Sci 12 (2): 179-184. DOI: 10.1016/j.jds.2016.09.005.
Luo S, Wang Y, Kang X, Liu P, Wang G. 2022. Research progress on the association between mastitis and gastrointestinal microbes in dairy cows and the effect of probiotics. Microb Pathog 173: 105809. DOI: 10.1016/j.micpath.2022.105809.
McGovern CJ, González-Orozco BD, Jiménez-Flores R. 2024. Evaluation of kefir grain microbiota, grain viability, and bioactivity from fermenting dairy processing by-products. J Dairy Sci 107 (7): 4259-4276. DOI: 10.3168/jds.2023-24364.
Mgomi FC, Yang Y-ran, Cheng G, Yang ZQ. 2023. Lactic acid bacteria biofilms and their antimicrobial potential against pathogenic microorganisms. Biofilm 5: 100118. DOI: 10.1016/j.bioflm.2023.100118.
Mukhlisah AN, Arief II, Taufik E. 2017. Physical, microbial, and chemical qualities of dangke produced by different temperatures and papain concentrations. Med Pet 40 (1): 63-70. DOI: 10.5398/medpet.2017.40.1.63.
Nemati V, Hashempour-baltork F, Alizadeh AM, Varzakas T. 2023. Production of traditional torba yogurt using lactic acid bacteria isolated from fermented vegetables: Microbiological, physicochemical and sensory properties. J Agric Food Res 14: 100850. DOI: 10.1016/j.jafr.2023.100850.
Ningtyas DW, Tam B, Bhandari B, Prakash S. 2021. Effect of different types and concentrations of fat on the physico-chemical properties of soy protein isolate gel. Food Hydrocoll 111: 106226. DOI: 10.1016/j.foodhyd.2020.106226.
Nur F, Hatta M, Natzir R, Dijde MN. 2017. Isolation of lactic acid bacteria as a potential probiotic in dangke, a traditional food from Enrekang, Indonesia. Intl J Sci: Basic Appl Res 35 (1): 19-27.
Rahman MS, Emon DD, Nupur AH, Mazumder MAR, Iqbal A, Alim MA. 2024. Isolation and characterization of probiotic lactic acid bacteria from local yogurt and development of inulin-based synbiotic yogurt with the isolated bacteria. Appl Food Res 4 (2): 100457. DOI: 10.1016/j.afres.2024.100457.
Sabil S, Malaka R, Maruddin F. 2017. Chemical and microstructure characteristics of dangke at various of temperature ripened. Intl J ChemTech Res 10 (7): 611-615.
Sakandar HA, Zhang H. 2021. Trends in probiotic(s)-fermented milks and their in vivo functionality: A review. Trends Food Sci Technol 110: 55-65. DOI: 10.1016/j.tifs.2021.01.054.
Savaiano DA, Hutkins RW. 2021. Yogurt, cultured fermented milk, and health: A systematic review. Nut Rev 79 (5): 599 -614.
Sfakianakis P, Tzia C. 2014. Conventional and innovative processing of milk for yogurt manufacture; development of texture and flavor: A review. Foods 3 (1): 176-193. DOI: 10.3390/foods3010176.
Sharma H, Ozogul F, Bartkiene E, Rocha JM. 2023. Impact of lactic acid bacteria and their metabolites on the techno-functional properties and health benefits of fermented dairy products. Critical Rev Food Sci Nutr 63 (21): 4819-4841. DOI: 10.1080/10408398.2021.2007844.
Sintasari RA, Kusnadi J, Ningtyas DW. 2014. Pengaruh penambahan konsentrasi susu skimmed dan sukrosa terhadap karakteristik minuman probiotik sari beras merah. J Pangan Agro 2 (3): 65-75. [Indonesian]
Su Y, Wang H, Wu Z, Zhao L, Huang W, Shi B, He J, Wang S, Zhong K. 2022. Sensory description and consumer hedonic perception of ultra-high temperature (UHT) milk. Foods 11 (9): 1350. DOI: 10.3390/foods11091350.
Surajudin, Kusuma F, Purnomo D. 2008. Yoghurt: Susu Fermentasi yang Menyehatkan. AgroMedia, Jakarta Selatan. [Indonesian]
Syah SP, Sumantri C, Arief II, Taufik E. 2017a. Isolation and identification of indigenous lactic acid bacteria by sequencing the 16s rRNA from dangke, a traditional cheese from Enrekang, South Sulawesi. Pak J Nutr 16: 384-392. DOI: 10.3923/pjn.2017.384.392.
Syah SP, Sumantri C, Arief II, Taufik E. 2017b. Karakteristik minuman whey yang difermentasikan dengan bakteri asam laktat indigenus asal dangke. Jurnal Teknologi dan Industri Pangan 28 (2): 129-138. DOI: 10.6066/jtip.2017.28.2.129. [Indonesian]
Tan C, Tian Y, Tao L, Xie J, Wang M, Zhang F, Yu Z, Sheng J, Zhao C. 2024. Exploring the effect of milk fat on fermented milk flavor based on gas chromatography-ion mobility spectrometry (GC-IMS) and multivariate statistical analysis. Molecules 29 (5): 1099. DOI: 10.3390/molecules29051099.
Tavakoli M, Habibi NMB, Mohebbi M. 2019. Effect of the milk fat content and starter culture selection on proteolysis and antioxidant activity of probiotic yogurt. Heliyon 5 (2): e01204. DOI: 10.1016/j.heliyon.2019.e01204.
Vareltzis P, Adamopoulos K, Stavrakakis E, Stefanakis A, Goula AM. 2016. Approaches to minimise yoghurt syneresis in simulated tzatziki sauce preparation. Intl J Dairy Technol 69 (2): 191-199. DOI: 10.1111/1471-0307.12238.
Vicent V. 2024. Influence of banana powder on proximate composition, physicochemical and rheological properties of soy yoghurt. Appl Food Res 4 (2): 100450. DOI: 10.1016/j.afres.2024.100450.
Walstra P, Wouters JTM, Geurts TJ. 2006. Dairy Science and Technology 2nd Edition. Taylor and Francis Group, LLC, Wageningen. DOI: 10.1201/9781420028010.
Wang MS, Fan M, Zheng AR, Wei CK, Liu DH, Thaku K, Wei ZJ. 2023a. Characterization of a fermented dairy, sour cream: Lipolysis and the release profile of flavor compounds. Food Chem 423: 136299. DOI: 10.1016/j.foodchem.2023.136299.
Wang N, Wang J, Wu P, Cong H, Hernalsteens S, Chen XD. 2023b. Conceptualizing ‘food parcel for colon microbes’ designed for delivering to human colon which assists the growth of the probiotics there: An exploratory in vitro study with Lactobacillus rhamnosus GG and Bifidobacterium animalis subsp. lactis BB-12’. Food Biosci 56: 103226. DOI: 10.1016/j.fbio.2023.103226.
Widodo W. 2002. Bioteknologi Fermentasi Susu. Pusat Pengembangan Bioteknologi Universitas Muhammadiyah Malang, Malang. [Indonesian]
Wirdayat W, Rosida R. 2024. Characteristics of juwet fruit yoghurt with the addition of skimmed milk and lactic acid bacteria starter. Asian J Appl Res Community Dev Empowerment 8 (3): 180-184. DOI: 10.29165/ajarcde.v8i3.488.
Wu H, Dong JJ, Dai YQ, Liu XL, Zhou JZ, Xia XD. 2021. Effects of lactic acid bacteria fermented yellow whey on the protein coagulation and isoflavones distribution in soymilk. Food Chem 334: 127484. DOI: 10.1016/j.foodchem.2020.127484.
Xu X, Cui H, Xu J, Yuan Z, Liu X, Fan X, Li J, Zhu D, Liu H. 2022. Effects of different probiotic fermentations on the quality, soy isoflavone and equol content of soy protein yogurt made from soy whey and soy embryo powder. LWT 157: 113096. DOI: 10.1016/j.lwt.2022.113096.
Yang S, Xu X, Peng Q, Ma L, Qiao Y, Shi B. 2023a. Exopolysaccharides from lactic acid bacteria, as an alternative to antibiotics, on regulation of intestinal health and the immune system. Anim Nutr 13: 78-89. DOI: 10.1016/j.aninu.2023.02.004.
Yang X, Ke C, Li L. 2021. Physicochemical, rheological and digestive characteristics of soy protein isolate gel induced by lactic acid bacteria. J Food Eng 292: 110243. DOI: 10.1016/j.jfoodeng.2020.110243.
Yang Y, Xia Y, Wang YR, Sun LS, Shuang Q, Zhang FM. 2023b. Optimization of lactic acid bacterial starter culture to improve the quality and flavor characteristics of traditional Hurood. J Dairy Sci 107 (1): 105-122. DOI: 10.3168/jds.2023-23754.
Yeboah PJ, Wijemanna ND, Eddin AS, Williams LL, Ibrahim SA. 2023. Lactic Acid Bacteria: Review on the potential delivery system as an effective probiotic. In: Ibrahim SA (eds). Dairy Processing-From Basics to Advances. IntechOpen. DOI: 10.5772/intechopen.111776.
Yousef AE, Carlstrom C. 2003. Food Microbiology: A Laboratory Manual. John Wiley and Sons, Ohio.
Yu Y, Xu Y, Li L,Chen S, An K, Yu Y, Xu ZL. 2023. Isolation of lactic acid bacteria from Chinese pickle and evaluation of fermentation characteristics. Lwt 180: 114627. DOI: 10.1016/j.lwt.2023.114627.
Yusuf M, Nur FUA, Syahriati, Saleh R, Tasir, Syamsuar. 2022. Dangke: Local indigenous cheese from Enrekang, South Sulawesi Indonesia. IOP Conf Ser: Earth Environ Sci 1097: 12064. DOI: 10.1088/1755-1315/1097/1/012064.
Zakariah MA, Malaka R, Laga A, Ako A. 2019. Isolation and identification of lactic acid bacteria from dangke a white soft traditional cheese from Enrekang Regency. Intl J Recent Technol Eng 8 (2): 4148-4151. DOI: 10.35940/ijrte.B3160.078219.
Zakariah MA, Malaka R, Laga A, Ako A. 2022. Quality and storage time of traditional dangke cheese inoculated with indigenous lactic acid bacteria isolated from Enrekang District, South Sulawesi, Indonesia. Biodiversitas 23 (6): 3270-3276. DOI: 10.13057/biodiv/d230656.
Zare F, Boye JI, Orsat V, Champagne C, Simpson BK. 2011. Microbial, physical and sensory properties of yogurt supplemented with lentil flour. Food Res Intl 44: 2482-2488. DOI: 10.1016/j.foodres.2011.01.002.
Zhang J, Xiao Y, Wang H, Zhang H, Chen W, Lu W. 2023. Lactic acid bacteria-derived exopolysaccharide: Formation, immunomodulatory ability, health effects, and structure-function relationship. Microbiol Res 274: 127432. DOI: 10.1016/j.micres.2023.127432.
Zhao Y, Khalesi H, He J, Fang Y. 2023. Application of different hydrocolloids as fat replacer in low-fat dairy products: Ice cream, yogurt and cheese. Food Hydrocoll 138: 108493. DOI: 10.1016/j.foodhyd.2023.108493.