Assessment of fungal contamination in dangke, a traditional cheese product from small-scale producers in Enrekang, South Sulawesi
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Abstract. Syah SP, Sudarwanto MB, Sudirman I, Mukhlisah AN, Ningtyas WD, Irfan M. 2025. Assessment of fungal contamination in dangke, a traditional cheese product from small-scale producers in Enrekang, South Sulawesi. Biodiversitas 26: 3324-3333. Dangke is a traditional soft cheese produced in Enrekang, South Sulawesi, Indonesia, using household-scale methods that can pose significant food safety risks due to inadequate hygiene practices. This study assessed the level and diversity of fungal contamination in dangke collected from small-scale producers across Enrekang Regency. A total of 20 samples were collected aseptically and analyzed using the Total Plate Count (TPC) method on Sabouraud Dextrose Agar. The results showed that the average fungal count was 5.2 × 10? CFU/g, which greatly exceeded the maximum acceptable limit for solid dairy products (10 CFU/g) according to Codex Alimentarius guidelines. Phenotypic identification revealed the presence of both yeasts (75%) and molds (25%), with nine fungal genera detected. Candida sp. was the dominant genus (30%), followed by Saccharomyces sp. (16%), Geotrichum sp. (11%), Microsporum sp. (13%), Cladosporium sp. (9%), Penicillium sp. (9%), Aspergillus sp. (6%), Rodotorula sp. (5%), and Fusarium sp. (2%). Several of these genera, such as Aspergillus and Fusarium, are known producers of mycotoxins that pose potential health risks. These findings highlight the urgent need for improved hygiene practices, proper handling, and better storage conditions in traditional dangke production to reduce fungal contamination and ensure product safety for consumers.
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References
Aasa AO, Fru FF, Adelusi OA, Oyeyinka SA, Njobeh PB. 2023. A review of toxigenic fungi and mycotoxins in feeds and food commodities in West Africa. World Mycotoxin J 16 (1): 33-47. DOI: 10.3920/WMJ2021.2766.
Ahmed AE, AL-Kahtani MM, El-Diasty EM, Ahmed AS, Saber H, Abbas AM, Diab HM, Alshehri MA, Elmansi AA, Hussein MA. 2020. Diversity of toxigenic molds and mycotoxins isolated from dairy products: Antifungal activity of Egyptian marine algae on Aspergillus and Candida species. J Pure Appl Microbiol 14 (1): 215-232. DOI: 10.22207/JPAM.14.1.23.
Ahmed SA, de Hoog S. 2023. Basic mycology. In: Hospenthal DR, Rinaldi MG, Walsh TJ (eds). Diagnosis and Treatment of Fungal Infections. Springer International Publishing, Switzerland. DOI: 10.1007/978-3-031-35803-6_2.
Akinyemi MO, Ezeokoli OT, Adeleke RA, Ezekiel CN. 2023. Bacterial and yeast communities in raw milk from three dairy animal species in Nigeria. Lett in Appl Microbiol 76 (1): ovac010. DOI: 10.1093/lambio/ovac010.
Alhussein HA, Al-Yasiri MH. 2019. Candidiasis. Ministry of Higher Education and Scientific Research. Thi-Qar University, Dhi Qar Governorate, Irak. http://biologydept.com/upload/upfile/ar/58.pdf
Alonso-Monge R, Gresnigt MS, Román E, Hube B, Pla J. 2021. Candida albicans colonization of the gastrointestinal tract: A double-edged sword. PLoS Pathog 17: e1009710. DOI: 10.1371/journal.ppat.1009710.
Aranda C, Rodriguez R, Fernández-Baldo MA, Durán P. 2025. Mycotoxins in cheese: Assessing risks, fungal contaminants, and control strategies for food safety. Foods 14 (3): 351. DOI: 10.3390/foods14030351.
Arechavala A, Negroni R, Santiso G Depardo R, Bonvehí P. 2021. Chronic recurrent vulvovaginitis is not only due to Candida. Rev Iberoam Micol 38 (3): 132-137. DOI: 10.1016/j.riam.2021.03.002.
Awasti N, Anand S. 2020. The role of yeast and molds in dairy industry: An update. Dairy Proc: Adv Res Appl: 243-262. DOI: 10.1007/978-981-15-2608-4_12
Barnett HL, Hunter BB. 1972. Illustrated Genera of Imperfect Fungi. Burgess Publishing Company, Minneapolis. DOI: 10.2307/3757954.
Bintsis T. 2017. Foodborne pathogens. AIMS Microbiol 3 (3): 529-563. DOI: 10.3934/microbiol.2017.3.529.
Cauchie M, Desmet S, Lagrou K. 2017. Candida and its dual lifestyle as a commensal and a pathogen. Res microbiol 168 (9-10): 802-810. DOI: 10.1016/j.resmic.2017.02.005.
Chandran A, Linsha CK, Beena AK. 2022. Prevalence of Candida species in 'Thairu'a traditional fermented milk of Kerala, India. Eur J Nutr Food Saf 14 (11): 15-19. DOI: 10.9734/ejnfs/2022/v14i111261.
Chen B, Sun Y, Luo F, Wang C. 2020. Bioactive metabolites and potential mycotoxins produced by cordyceps fungi: A review of safety. Toxins 12 (6): 1-13. DOI: 10.3390/toxins12060410.
Ciurea CN, Kosovski IB, Mare AD, Toma F, Pintea-Simon IA, Man A. 2020. Candida and candidiasis opportunism versus pathogenicity: A review of the virulence traits. Microorganisms 8 (6): 1-17. DOI: 10.3390/microorganisms8060857.
Department of Defense Food Safety and Quality Assurance Laboratory Action Levels. 2016. USAPHC Circular 40-1, Appendix O. https://sph.health.mil/approved.nsf/86257B8D004A4B6C/0/C55916ED3005D38D862573E9005A98B8?OpenDocument
Dey DK, Kang JI, Bajpai VK, Kim K, Lee H, Sonwal S, Simal-Gandara J, Xiao J, Ali S, Huh YS, Han Y-K, Shukla S. 2023. Mycotoxins in food and feed: Toxicity, preventive challenges, and advanced detection techniques for associated diseases. Crit Rev Food Sci Nutr 63 (27): 8489-8510. DOI: 10.1080/10408398.2022.2059650.
Ellis DH, Davis S, Alexiou H, Handke R, Bartley R. 2007. Descriptions of Medical Fungi (2nd ed.). School of Molecular & Biomedical Science University of Adelaide, Australia.
El-Sayed AS, Ibrahim H, Farag MA. 2022. Detection of potential microbial contaminants and their toxins in fermented dairy products: A comprehensive review. Food Anal Methods 15 (7): 1880-1898. DOI: 10.1007/s12161-022-02253-y.
Espinosa-Hernández VM, Morales-Pineda V, Martínez-Herrera E. 2020. Skin infections caused by emerging candida species. Curr Fungal Infec Rep 14: 99-105. DOI: 10.1007/s12281-020-00380-9.
FAO-WHO. 2011. Milk and Milk Products (2nd ed.). FAO and WHO, Rome.
Fisher FW, Cook N. B. 1999. Fundamentals of Diagnostic Mycology. WB Saunders Company, Philadelphia. DOI: 10.5858/123.6.545b
Fleet GH, Mian MA. 1987. The occurrence and growth of yeasts in dairy products. Intl J Food Microbiol 4 (2): 145-155. DOI: 10.1016/0168-1605(87)90021-3.
Fleet GH. 1990. Yeasts in dairy products. J Appl Bacteriol 68 (3): 199-211.
Gadaga TH, Mutukumira AN, Narvhus JA. 2000. Enumeration and identification of yeasts isolated from Zimbabwean traditional fermented milk. Intl Dairy J 10: 459-466. DOI: 10.1016/S0958-6946(00)00070-4.
Gallo M, Ferrara L, Calogero A, Montesano D, Naviglio D. 2020. Relationships between food and diseases: What to know to ensure food safety. Food Res Int 137: 109414. DOI: 10.1016/j.foodres.2020.109414.
Garnier L, Valence F, Mounier J. 2017. Diversity and control of spoilage fungi in dairy products: An update. Microorganisms 5 (3): 1-33. DOI: 10.3390/microorganisms5030042.
Hatta W, Sudarwanto MB, Sudirman I, Malaka R. 2013. Prevalence and sources of contamination of Escherichia coli and Salmonella spp. in cow milk dangke, Indonesian fresh soft cheese. Glob Vet 11 (3): 352-356. DOI: 10.5829/idosi.gv.2013.11.3.7611.
Herlina VT, Setiarto RHB. 2024. From tradition to innovation: Dadih, the Minangkabau tribe's traditional fermented buffalo milk from Indonesia. J Ethnic Foods 11 (1): 1-16. DOI: 10.1186/s42779-024-00234-6.
Howell SA, Hazen K. C. 2022. Candida, cryptococcus, and other yeasts of medical importance. Manual Clin Microbiol 2 (2): 1793-1821. DOI: 10.1128/9781555816728.ch115.
Hymery N, Vasseur V, Coton M, Mounier J, Jany JL, Barbier G, Coton E. 2014. Filamentous fungi and mycotoxins in Cheese: A review. Compr Rev Food Sci Food Saf 13: 437-456. DOI: 10.1111/1541-4337.12069.
Izzo L, Mikušová P, Lombardi S, Sulyok M, Ritieni A. 2022. Analysis of mycotoxin and secondary metabolites in commercial and traditional Slovak cheese samples. Toxins 14: 134. DOI: 10.3390/toxins14020134.
Kalamaki MS, Angelidis AS. 2023. Growth and survival of Listeria monocytogenes during the manufacture and storage of artisanal kefir. Food Sci Technol Intl 29: 789-798. DOI: 10.1177/10820132221117462.
Kauffman CA. 2005. Candiduria. Clin Infec Dis 41: S371-S376. DOI: 10.1086/430918.
Kure CF, Skaar I. 2019. The fungal problem in cheese industry. Curr Opin Food Sci 29: 14-19. DOI: 10.1016/j.cofs.2019.07.003.
Long J, Du G, Chen J, Xie C, Xu J, Yuan J. 2023. Bacteria and poisonous plants/fungi were the primary causative hazards of foodborne disease outbreaks: A five-year survey from Guangzhou, Guangdong. IntL J Food Microbiol 400: 110264. DOI: 10.1016/j.ijfoodmicro.2023.110264.
Malaka R, Maruddin F, Baco S. 2023. Dangke: Keju Tradisional Enrekang, Sulawesi Selatan. Unhas Press, Makassar. [Indonesian]
Malaka R, Prahesti KI, Mahendradatta M, Astawan M, Putranto WS, Arief FA, Waqiah SN, Kadir RW. 2024. Physicochemical properties of dangke-cheese by different temperature processing and papaya-latex as coagulant. Indian J Agric Res 58 (5): 911-916. DOI: 10.18805/ijare.af-882.
Malik AW, Awad AK, Qaddoori HT. 2022. Candida species associated with urinary tract infections. World J Adv Res and Rev 16 (1): 111-121. DOI: 10.30574/wjarr.2022.16.1.1000.
Moubasher AAH, Abdel-Sater MA, Soliman ZSM. 2018. Yeasts and filamentous fungi associated with some dairy products in Egypt. J Mycolo Med 28(1): 76-86. DOI: 10.1016/j.mycmed.2017.12.003.
Ndudi W, Edo GI, Samuel PO, Jikah AN, Opiti RA, Ainyanbhor IE, Essaghah AEA, Ekokotu HA, Oghroro EAE, Agbo JJ. 2024. Traditional fermented foods of Nigeria: Microbiological safety and health benefits. J Food Meaa Char 18 (6): 4246-4271.
Ntuli V, Sibanda T, Elegbeleye JA, Mugadza DT, Seifu E, Buys EM. 2022. Dairy production: Microbial safety of raw milk and processed milk products. In: Knowles ME, Anelich L, Boobis A, Popping B (eds). Present Knowledge in Food Safety: A Risk-Based Approach through the Food Chain. Academic Press, Oxford. DOI: 10.1016/B978-0-12-819470-6.00076-7.
Oliveira M, Oliveira D, Lisboa C, Boechat JL, Delgado L. 2023. Clinical manifestations of human exposure to fungi. J Fungi 9 (3): 1-41. DOI: 10.3390/jof9030381.
Opiyo BA, Wangoh J, Njage PMK. 2013. Microbiological performance of dairy processing plants is influenced by scale of production and the implemented food safety management system: A case study. J Food Protec 76 (6): 975-983. DOI: 10.4315/0362-028X.JFP-12-450.
Pandey AK, Samota MK, Kumar A et al. 2023. Fungal mycotoxins in food commodities: Present status and future concerns. Front Sustain Food Syst 7: 1162595. DOI: 10.3389/fsufs.2023.1162595.
Rudramurthy SM, Singh S. 2020. Candida infections in immunocompetent hosts: Pathogenesis and diagnosis. Curr Fungal Infect Rep 14: 233-245. DOI: 10.1007/s12281-020-00392-5.
Ryu D, Wolf-Hall C. 2015. Yeasts and molds. In: Salfinger Y, Tortorello M (eds). Compendium of Methods for the Microbiological Examination of Foods: 21. APAH Press, Washington, DC.
Shekhar C. 2024. Influence of production processes on assessment of safety and quality of fermented milk. In: Rana T (eds). The Microbiology, Pathogenesis and Zoonosis of Milk Borne Diseases: Milk Hygiene in Veterinary and Public Health. Academic Press, Oxford. DOI: 10.1016/B978-0-443-13805-8.00010-7.
Shiawoya EL, Ijah UJJ, Olatunji EA, Lamidi AO. 2006. Occurrence of moulds and yeasts in wara (local cheese) preserved with whey permeate and salt solution. J Sustain Agric Environ 8 (1): 73-79.
Silva SPM, Teixeira JA, Silva CC. G. 2023. Prevention of fungal contamination in semi-hard cheeses by whey-gelatin film incorporated with Levilactobacillus brevis SJC120. Foods 12 (7): 1-18. DOI: 10.3390/foods12071396.
Singh S, Shivhare UD, Sakarkar SN. 2013. Oral infections, a mirror of our overall health condition: An overview. Intl J Pharm Life Sci 4 (9): 2976-2982.
Syah SP, Mukhlisah AN, Ningtiyas WD, Irfan M, Ananda N, Amalia A, Tasmin T. 2024. Physicochemical and microbiological properties of fermented milk using lactic acid bacteria isolated from dangke. Biodiversitas 25 (10): 3876-3883. DOI: 10.13057/biodiv/d251048.
Syah SP, Sumantri C, Arief II, Taufik E. 2017. Isolation and identification of indigenous lactic acid bacteria by sequencing the 16s rRNA from dangke, a traditional cheese from Enrekang, South Sulawesi. Pak J Nut 16 (5): 384-392. DOI: 10.3923/pjn.2017.384.392.
Syah SP. 2022. Isolasi dan Karakterisasi Bakteri Asam Laktat asal Dangke secara Molekuler serta Potensinya untuk Produksi Minuman Whey Fermentasi. PT. Nasya Expanding Management, Pekalongan. [Indonesian]
Taale E, Adjana PS, Gambogou B, Souho T. 2025. Assessment of microbial contamination in home-produced wagashi, a traditional cheese from Kozah municipalities in Togo. Afr J Microbiol Res 19 (5): 83-92.
Tebbi CK. 2023. Mycoviruses in fungi: Carcinogenesis of fungal agents may not always be mycotoxin related. J Fungi 9 (3): 1-17. DOI: 10.3390/jof9030368.
Terai H, Ueno T, Suwa Y, Omori M, Yamamoto K, Kasuya S. 2018. Candida is a protractive factor of chronic oral ulcers among usual outpatients. Japan Dent Sci Rev 54 (2): 52-58. DOI: 10.1016/j.jdsr.2017.12.001.
Torkar KG, Vengušt A. 2008. The presence of yeasts, molds and aflatoxin M1 in raw milk and cheese in Slovenia. Food Control 19 (6): 570-577. DOI: 10.1016/j.foodcont.2007.06.008
Vázquez-Olvera R, Volkow P, Velázquez-Acosta C, Cornejo-Juárez P. 2023. Candida bloodstream infection in patients with cancer: A retrospective analysis of an 11-year period. Rev Iberoam Micol 40 (1): 3-9. DOI: 10.1016/j.riam.2022.12.002.
Wang X, Zhang W, Wu W, Young A, Yan Z. 2023. Is Candida albicans a contributor to cancer? A critical review based on the current evidence. Microbiol Res 272: 127370. DOI: 10.1016/j.micres.2023.127370.
Weber RWS, Pitt D. 2000. Teaching techniques for mycology: 11. Riddell’s slide cultures. Mycologist 14 (3): 118-120. DOI: 10.1016/S0269-915X(00)80088-9.
Widyadhana NM, Bamahry AR, Kamaluddin IDK, Safitri A, Mangarengi Y. 2024. Identifikasi morfologi dan koloni bakteri probiotik pada makanan tradisional dangke. J Aafiyah Health Res 5 (1): 75-80. DOI: 10.52103/jahr.v5i1.1587. [Indonesian]
Wikandari R, Kinanti DA, Permatasari RD, Rahmaningtyas NL, Chairunisa NR, Sardjono, Hellwig C, Tahrezadeh MJ. 2021. Correlations between the chemical, microbiological characteristics and sensory profile of fungal fermented food. Fermentation 7 (4): 261. DOI: 10.3390/fermentation7040261.
Yahyapour G, Anvar SAA, Ataee M, Hamed HA, Askari H. 2023. Isolation, identification, and characterization of the native yeast strains from homemade cheese to assess their eliminating impact on the aflatoxin B1 and M1 of the simulated gastrointestinal fluid. Iranian J Biotechnol 21: 95-104. DOI: 10.30498/ijb.2023.330834.3291.
Zakariah MA, Malaka R, Laga A, Ako A, Mauliah FU, Zakariah M. 2019. Microbial contamination on dangke fresh white cheese from traditional home industry in Enrekang Regency. IOP Conf Ser Earth Environ Sci 247: 12048. DOI: 10.1088/1755-1315/247/1/012048.
Zakariah MA, Malaka R, Laga A, Akoa A, Zakariah M, Mauliah FU. 2022. Quality and storage time of traditional dangke cheese inoculated with indigenous lactic acid bacteria isolated from Enrekang District, South Sulawesi, Indonesia. Biodiversitas 23 (6): 3270-3276. DOI: 10.13057/biodiv/d230656.