Fertilization strategies alter growth and rhizosphere microbiome diversity in white potato (Solanum tuberosum)
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Abstract. Nikmatullah A, Sarjan M, Jihadi A, Nairfana I, Prasedya ES. 2026. Fertilization strategies alter growth and rhizosphere microbiome diversity in white potato (Solanum tuberosum). Biodiversitas 27 (1): d270135. https://doi.org/10.13057/biodiv/d270135. Excessive inorganic fertilization is widely used to boost potato productivity, but often reduces nutrient-use efficiency and disrupts soil microbial communities. This study evaluated how three fertilization regimes viz. CF100 (100% recommended inorganic + organic fertilizer), CF50 (50% recommended inorganic + organic fertilizer), and CF50OF (50% recommended inorganic + organic fertilizer + biofertilizer), affect plant growth, yield, and rhizosphere microbiome composition of white potato (Solanum tuberosum). Growth assessments at five weeks after shoot emergence showed that CF50OF produced significantly higher shoot numbers, fresh biomass, and dry biomass compared to CF50, and achieved growth comparable to CF100. Tuber yield did not differ significantly among treatments. Amplicon sequencing of rhizosphere soil revealed that fertilization strategy strongly shaped microbial richness, taxonomic composition, and phylogenetic structure. CF100 exhibited the highest observed taxa and phylogenetic diversity, whereas CF50OF enriched a broader spectrum of low-abundance and functionally diverse microbial genera associated with nutrient cycling, organic matter degradation, hormone production, biocontrol, abiotic stress adapters, and other functions. Despite lower Shannon and Simpson diversity values compared to CF100 and CF50, CF50OF supported microbial communities with greater functional potential, which may compensate for reduced inorganic inputs. These findings demonstrate that integrating biofertilizer with reduced inorganic fertilizer can sustain potato growth while promoting a more ecologically functional rhizosphere microbiome, offering a promising strategy for nutrient-efficient and environmentally sustainable potato cultivation.
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References
Abeytilakarathna PD. 2022. Factors affecting stolon formation and tuberization in potato: A review. Agric Rev 43 (1): 91-97. https://doi.org/10.18805/ag.R-187.
Al-Shammary AAG, Al-Shihmani LSS, Fernández-Gálvez J, Caballero-Calvo A. 2024. Optimizing sustainable agriculture: a comprehensive review of agronomic practices and their impacts on soil attributes. J Environ Manag 364: 121487. https://doi.org/10.1016/j.jenvman.2024.121487.
Barłóg P, Grzebisz W, Łukowiak R. 2022. Fertilizers and fertilization strategies mitigating soil factors constraining efficiency of nitrogen in plant production. Plants 11 (14): 1855. https://doi.org/10.3390/plants11141855
Beltrán-García MJ, Martínez-Rodríguez A, Olmos-Arriaga I, Valdés-Salas B, Mascio PD, White JF. 2021. Nitrogen fertilization and stress factors drive shifts in microbial diversity in soils and plants. Symbiosis 84: 379-390. https://doi.org/10.1007/s13199-021-00787-z.
Betancur CB, Lang B, Russell DJ. 2023. Organic nitrogen fertilization benefits selected soil fauna in global agroecosystems. Biol Fertil Soils 59: 1-162. https://doi.org/10.1007/s00374-022-01677-2.
BPS [Badan Pusat Statistik]. 2025. Produksi tanaman sayuran. https://www.bps.go.id/id/statistics-table/2/NjEjMg==/production-of-vegetables.html. [Indonesian]
Buckseth T, Kuma, V, Sharma AK, Sood S, Dalamu, Bhardwaj V, Sadawarti MJ, Challam C, Mangal V, Singh B. 2024. An overview of seed potato production: National and International perspectives. In: Buckseth T, Kumar V, Sharma AK (eds). Approaches for Potato Crop Improvement and Stress Management. Springer. https://doi.org/10.1007/978-981-97-1223-6_17.
Byappanahalli MN, Nevers MB, Korajkic A, Staley ZR, Harwood VJ. 2012. Enterococci in the environment. Microbiol Mol Biol Rev 76 (4): 685-706. https://doi.org/10.1128/MMBR.00023-12.
Chabani H, Tarchoun N, Amami R, Saadaoui W, Mezghani N, Alexopoulos AA, Petropoulos SA. 2025. Investigating the effects of optimized mineral fertilization on plant growth, physiological traits, tuber yield, and biochemical contents of the potato crop. Horticulturae 11 (1): 11. https://doi.org/10.3390/horticulturae11010011.
Chea L, Alhussein M, Karlovsky P, Pawelzik F, Naumann M. 2024. Adaptation of potato cultivars to phosphorus variability and enhancement of phosphorus efficiency by Bacillus subtilis. BMC Plant Biol 24: 1176. https://doi.org/10.1186/s12870-024-05868-x.
Chen Q, Song Y, An Y, Lu Y, Zhong G. 2024. Soil microorganisms: Their role in enhancing crop nutrition and health. Diversity 16 (12): 734. https://doi.org/10.3390/d16120734.
Chen Y, Li Q, Wu W, Liu X, Cheng J, Deng X, Cai X, Yuan W, Xie J, Zhang S, Wang B. 2022. Effects of lightning on rhizosphere soil properties, bacterial communities, and active components of Camellia sinensis var. assamica. Front Microbiol 13: 911226. https://doi.org/10.3389/fmicb.2022.911226.
Chhetri G, Kim I, Kang M, Kim J, So Y, Seo T.2022. Devosia rhizoryzae sp. nov., and Devosia oryziradicis sp. nov., novel plant growth-promoting members of the genus Devosia, isolated from the rhizosphere of rice plants. J Microbiol 60: 1-10. https://doi.org/10.1007/s12275-022-1474-8.
Chiodi C, Zardinoni G, Stevanato P, Giagnoni L, Carletti P, Oustrière N, Sæbø A, Persson T, Szulc W, Rutkowska B, Mench M, Renella G. 2025. Organic amendments influence soil properties, microbial diversity, and winter barley traits in a five-year field trial. Chem Biol Technol Agric 12: 87. https://doi.org/10.1186/s40538-025-00810-1.
de Jesus Colwell F, Souter J, Bryan GJ, Compton LJ, Boonham N, Prashar A. 2021. Development and validation of methodology for estimating potato canopy structure for field crop phenotyping and improved breeding. Front Plant Sci 12: 612843. https://doi.org/10.3389/fpls.2021.612843.
Dong M, Hu S, Lv S, Rong F, Wang X, Gao X, Xu Z, Xu Y, Liu K, Liu A. 2022. Recovery of microbial community in strongly alkaline bauxite residues after amending biomass residue. Ecotoxicol Environ Saf 232: 113281. https://doi.org/10.1016/j.ecoenv.2022.113281.
Dzvene AR, Chiduza C. 2024. Application of biofertilizers for enhancing beneficial microbiomes in push-pull cropping systems: A review. Bacteria 3: 271-286. https://doi.org/10.3390/bacteria3040018.
Fang X, Xiang Z, Ma H, Wang F, Wang Q, Li P, Zheng S. 2023. Effect of N fertilizer dosage and base/topdressing ratio on potato growth characteristics and yield. Agronomy 13 (3): 909. https://doi.org/10.3390/agronomy13030909.
FAO [Food and Agriculture Organization]. 2023. FAOSTAT. Food and Agriculture Organization of the United Nations. Retrieved from https://www.fao.org/faostat/en.
Gao W, Chen S, Yu X, Chen S, Wan C, Wang Y, Wu P, Li Q. 2025. Three local plants adapt to the ecological restoration of abandoned lead-zinc mines through the assembly of rhizosphere bacterial communities. Front Microbiol 16: 1533965. https://doi.org/10.3389/fmicb.2025.1533965.
Geng Y, Ding Y, Zhou P, Wang P, Peng C, Li D. 2024. Soil microbe-mediated carbon and nitrogen cycling during primary succession of biological soil crusts in tailings ponds. Sci Total Environ 894: 164969. https://doi.org/10.1016/j.scitotenv.2023.164969.
Gunadi N, Pronk A. 2023. Identifying key factors to improve productivity and reduce the environmental impact of potato farms in West Java, Indonesia. E3S Web Conf 373: 04019. https://doi.org/10.1051/e3sconf/202337304019.
Guo L, Nie Z, Zhou J, Zhang S, An F, Zhang L, Tóth T, Yang F, Wang Z. 2022. Effects of different organic amendments on soil improvement, bacterial composition, and functional diversity in saline-sodic soil. Agronomy 12 (10): 2294. https://doi.org/10.3390/agronomy12102294.
Han X, Yang J, Li Q, Zhang L, Li Y, Gai Y, Sang Y and Zhang Z. 2025. Organic fertilizer application rates affect rhizosphere microbial communities and yield optimization in potato (Solanum tuberosum L. V7). Front Microbiol 16: 1651178. https://doi.org/10.3389/fmicb.2025.1651178.
Hu HJ, Xu K, He LC. Wang GX. 2021. A model for the relationship between plant biomass and photosynthetic rate based on nutrient effects. Ecosphere 12 (8): e03678. https://doi.org/10.1002./ecs2.3678.
Hu M, Chen HY, Chang SX, Leuzinger S, Dukes JS, Langley JA, Bader MF, VanSundert K, Liao H, Ma Z. 2025. Plant functional traits affect biomass responses to global change: A meta-analysis. J Ecol 113: 2046-2065. https://doi.org/10.1111/1365-2745.70076.
Jennings SA, Koehler AK, Nicklin KJ, Deva C, Sait SM, Challinor AJ. 2020. Global potato yields increase under climate change with adaptation and CO2 fertilisation. Front Sustain Food Syst 4: 519324. https://doi.org/10.3389/fsufs.2020.519324.
Ji L, Ni K, Wu Z, Zhang J, Yi X, Yang X, Ling N, You Z, Guo S, Ruan J. 2020a. Effect of organic substitution rates on soil quality and fungal community composition in a tea plantation with long-term fertilization. Biol Fertil Soils 56: 633-646. https://doi.org/10.1007/s00374-020-01439-y.
Ji Y, Conrad R, Xu H. 2020b. Responses of archaeal, bacterial, and functional microbial communities to growth season and nitrogen fertilization in rice fields. Biol Fertil Soils 56: 81-95
Jiao S, Peng Z, Qi J, Gao J, Wei G. 2021. Linking bacterial-fungal relationships to microbial diversity and soil nutrient cycling. mSystems 6 (2): e01052-20. https://doi.org/10.1128/mSystems.01052-20.
Kargapolova KY, Burygin GL, Tkachenko OV, Evseeva NV, Belimov AA. 2020. Effectiveness of inoculation of in vitro-grown potato microplants with Azospirillum spp. Plant Cell Tissue Organ Cult 141: 351-359. https://doi.org/10.1007/s11240-020-01791-9.
Khan A, Yan L, Hasan MD, Wang W, Xu K, Zou G, Liu XD, Fang XW. 2022. Leaf traits and leaf nitrogen shift photosynthesis adaptive strategies among functional groups and diverse biomes. Ecol Indic 141: 109098. https://doi.org/10.1016/j.ecolind.2022.109098.
Kim HS, Lee SH, Jo HY, Finneran KT, Kwon MJ. 2021. Diversity and composition of soil Acidobacteria and Proteobacteria communities as a bacterial indicator of past land-use change from forest to farmland. Sci Total Environ 797: 148944. https://doi.org/10.1016/j.scitotenv.2021.148944.
Kirchgesser J, Hazarika M, Bachmann-Pfabe S, Dehmer KJ, Kavka M, Uptmoor R. 2023. Phenotypic variation of root-system architecture under high P and low P conditions in potato (Solanum tuberosum L.). BMC Plant Biol 23: 68. https://doi.org/10.1186/s12870-023-04070-9.
Kondhare KR, Kumar A, Patil NS, Malankar NM, Saha K, Banerjee AK. 2021. Development of aerial and below-ground tubers in potato is governed by photoperiod and epigenetic mechanisms. Plant Physiol 187 (3): 1071-1086. https://doi.org/10.1093/plphys/kiab409.
Kumar M, Ansari WA, Zeyad MT, Singh A, Chakdar H, Kumar A, Farooqi MS, Sharma A, Srivastava S, Srivastava AK. 2023. Core microbiota of wheat rhizosphere under Upper Indo-Gangetic plains and their response to soil physicochemical properties. Front Plant Sci 14:1186162. https://doi.org/10.3389/fpls.2023.1186162.
Li S, Tang S, Ju X, Zhu Z, Zhang Y, Chen H, Jin K. 2024. Soil acidification drives the negative effects of nitrogen enrichment on soil microbial biomass at the global scale. Plant Soil 503: 517-528. https://doi.org/10.1007/s11104-024-06600-2.
Li Y, Zhang X, Chen H. 2025. The invisible architects: Microbial communities and their transformative role in soil health. Environ Microbiome 20: 45-58. https://doi.org/10.1186/s40793-025-00694-6.
Lian T, Yin F, Cao Q, Zhou T, Zhang F, Sun X, Cai Y, Dong H. 2025. Enhancing lactic acid production by organic loading rate regulation during anaerobic co-fermentation: Insights into metabolic and bacterial responses. Environ Technol Innov 40: 104525. https://doi.org/10.1016/j.eti.2025.104525.
Liang Y, Wang Z, Gao N, Qi X, Zeng J, Cui K, Lu W, Bai S. 2024. Variations and interseasonal changes in the gut microbial communities of seven wild fish species in a natural lake with limited water exchange during the closed fishing season. Microorganisms 12 (4): 800. https://doi.org/10.3390/microorganisms12040800.
Liu H, Li S, Qiang R, Lu E, Li C, Zhang J, Gao Q. 2022. Response of soil microbial community structure to phosphate fertilizer reduction and combinations of microbial fertilizers. Front Environ Sci 10: 899727. https://doi.org/10.3389/fenvs.2022.899727.
Liu Q, Zong C, Dong Z, X, Zhu J, Li J, Zhang J, Saho T. 2020. Effects of cellulolytic lactic acid bacteria on the lignocellulose degradation, sugar profile, and lactic acid fermentation of high-moisture alfalfa ensiled in low-temperature seasons. Cellulose 27: 7955-7965. https://doi.org/10.1007/s10570-020-03350-z.
Lombardo S, Pandino G, Mauromicale G. 2020. Optimizing nitrogen fertilization to improve qualitative performances and physiological and yield responses of potato (Solanum tuberosum L.). Agronomy 10 (3): 352. https://doi.org/10.3390/agronomy10030352.
Luo J, Banerjee S, Ma Q, Liao G, Hu B, Zhao H, Li T. 2023. Organic fertilization drives shifts in microbiome complexity, and keystone taxa increase the resistance of microbial-mediated functions to biodiversity loss. Biol Fertil Soils 59: 441-458 https://doi.org/10.1007/s00374-023-01719-3.
Makani M, Zotarelli L, Sargent S, Huber D, Sims C. 2020. Nitrogen fertilizer rate affects yield and tuber quality of drip-irrigated tablestock potatoes (Solanum tuberosum L.) grown under subtropical conditions. Am J Potato Res 97: 605-614.
Mehmood N, Saeed M, Zafarullah S, Hyder S, Rizvi ZF, Gondal AS, Jamil N, Iqbal R, Ali B, Ercisli S, Kupe M. 2023. Multifaceted impacts of plant-beneficial Pseudomonas spp. in managing various plant diseases and crop yield improvement. ACS Omega 8 (25): 22296-22315. https://doi.org/10.1021/acsomega.3c00870.
Murindangabo YT, Kopecký M, Perná K, Nguyen TG, Konvalina P, Kavková M. 2023. Prominent use of lactic acid bacteria in soil-plant systems. Appl Soil Ecol 189: 104955. https://doi.org/10.1016/j.apsoil.2023.104955.
Na HS, Song Y, Yu Y, Chung J. 2023. Comparative analysis of primers used for 16S rRNA gene sequencing in oral microbiome studies. Methods Protoc 6 (4): 71. https://doi.org/10.3390/mps6040071.
Naqqash T, Malik KA, Imran A, Hameed S, Shahid M, Hanif MK, Majeed A, Iqbal MJ, Qaisrani MM, van Elsas JD. 2022. Inoculation with Azospirillum spp. acts as the liming source for improving the growth and nitrogen use efficiency of potato. Front Plant Sci 13: 929114. https://doi.org/10.3389/fpls.2022.929114.
Neilson JAD, Smith AM, Mesina L, Vivian R, Smienk S, Koyer DeD. 2021. Potato tuber shape phenotyping using RGB imaging. Agronomy 11: 1781. https://doi.org/10.3390/agronomy11091781.
Ng FL, Li TC, Wang E, Lee TY, Chen GT, Su JF, Chen WL. 2025. Bacillus-based biofertilizer influences soil microbiome to enhance soil health for sustainable agriculture. Sustainability 17: 6293. https://doi.org/10.3390/su17146293.
Nievas S, Coniglio A, Takahashi WY, López GA, Larama G. 2023. Unraveling Azospirillum's colonization ability through microbiological and molecular evidence. J Appl Microb 134 (4): lxad071. https://doi.org/10.1093/jambio/lxad071.
Nurhidayah N, Nikmatullah A, Haryanto H. 2023. Efektivitas pupuk hayati bactoplus dalam meningkatkan efisiensi pemupukan npk pada tanaman kentang (Solanum tuberosum L.) varietas Chitra. Jurnal Sains Teknologi Lingkungan 9 (3): 391-400. https://doi.org/10.29303/jstl.v9i3.472.
Pantelides IS, Stringlis IA, Finkel OM, Mercado-Blanco J. 2023. Organic amendments-microbial communities and their role in plant fitness and disease suppression. Front Plant Sci 14: 1213092. https://doi.org/10.3389/fpls.2023.1213092.
Prasedya ES, Kurniawan NSH, Kirana IAP, Ardiana N, Abidin AS, Ilhami BTK, Jupri A, Widyastuti, Sunarpi H, Nikmatullah A. 2022. Seaweed fertilizer prepared by em-fermentation increases the abundance of beneficial soil microbiome in paddy (Oryza sativa L.) during the vegetative stage. Fermentation 8 (2): 46. https://doi.org/10.3390/fermentation8020046.
Pronk AA, Gunadi N, Hermelink MI, Hengsdijk H, Jindo K, Silva JV. 2025. Opportunities to narrow potato yield gaps and increase resource use efficiency in West Java, Indonesia. Potato Res 68: 1137-1158. https://doi.org/10.1007/s11540-024-09778-1.
Raman J, Kim JS, Choi KR, Eun H, Yang D, Ko YJ, Kim SJ. 2022. Application of lactic acid bacteria (LAB) in sustainable agriculture: Advantages and limitations. Intl J Mol Sci 23: 7784. https://doi.org/10.3390/ijms23147784.
Ran T, Li J, Liao K, Zhao Y, Yang G, Long J. 2023. Effects of biochar amendment on bacterial communities and their function predictions in a microplastic-contaminated Capsicum annuum L. Soil Environ Technol Innov 31: 103174. https://doi.org/10.1016/j.eti.2023.103174.
Ren J, Shen YC, Shen FP. 2025. Optimizing fertilization strategies for high-yield potato crops. Mol Soil Biol 16 (1): 1-15. https://doi.org/10.5376/msb.2025.16.0001.
Sah S, Krishnani S, Singh R. 2021. Pseudomonas mediated nutritional and growth promotional activities for sustainable food security. Curr Res Microb Sci 2: 100084. https://doi.org/10.1016/j.crmicr.2021.100084.
Sharma R, Kumar D, Kapoor N, Ohri P. 2025. Insights into the biodiversity, mechanisms, and plant growth-promoting effects of Bradyrhizobium and their potential in sustainable agriculture. Pedosphere 2025. https://doi.org/10.1016/j.pedsph.2025.06.017.
Singh P, Arif Y, Siddiqu H, Upadhyaya CP, Pichtel J, Haya S. 2023. Critical factors responsible for potato tuberization. Bot Rev 89: 421-437. https://doi.org/10.1007/s12229-023-09289-7.
Song B, Wang T, Wan C, Cai Y, Mao L, Ge Z, Yang N. 2024. Diversity patterns and drivers of soil bacterial and fungal communities in a muddy coastal wetland of China. J Fungi 10 (11): 770. https://doi.org/10.3390/jof10110770.
Sunthornthummas S, Wasitthankasem R, Phokhaphan P, Sudtachat N, Wilantho A, Ngamphiw C, Chareanchim W, and Tongsima S. 2025. Unveiling the impact of 16S rRNA gene intergenomic variation on primer design and gut microbiome profiling. Front Microbiol 16: 1573920. https://doi.org/10.3389/fmicb.2025.1573920.
Tang J, Su L, Fang Y, Wang C, Meng L, Wang J, Zhang J, Xu W. 2023. Moderate nitrogen reduction increases nitrogen use efficiency and positively affects microbial communities in agricultural soils. Agriculture 13 (4): 796. https://doi.org/10.3390/agriculture13040796.
Tiwari JK, Buckseth T, Singh RK, Kumar M, Kant S. 2020. Prospects of improving nitrogen use efficiency in potato: Lessons from transgenics to genome editing strategies in plants. Front Plant Sci 11: 597481. https://doi.org/10.3389/fpls.2020.597481.
Wagg C, Schlaeppi K, Banerjee S, Kuramae EK, van der Heijden MGA. 2019. Fungal-bacterial diversity and microbiome complexity predict ecosystem functioning. Nat Commun 10: 4841. https://doi.org/10.1038/s41467-019-12798-y.
Wang H, Yang Y, Yao C, Feng Y, Wang H, Kong Y, Riez U, uz Zaman Q, Fahad S, Deng G. 2024. The correct combination and balance of macronutrients nitrogen, phosphorus, and potassium promote plant yield and quality through enzymatic and antioxidant activities in potato. J Plant Growth Regul 43: 4716-4734. https://doi.org/10.1007/s00344-024-11428-2.
Wang X, Wang X, Sheng H, Wang X, Zhao H, Feng K. 2022. Excessive nitrogen fertilizer application causes rapid degradation of greenhouse soil in China. Pol J Environ Stud 31: 1527-1534. https://doi.org/10.15244/pjoes/143293.
Xiao Z, Zhang S, Yan P, Huo J, Aurangzeib M. 2022. Microbial community and their potential functions after natural vegetation restoration in gullies of farmland in Mollisols of Northeast China. Land 11 (12): 2231. https://doi.org/10.3390/land11122231.
Xing Y, Xie Y, Wang X. 2025. Enhancing soil health through balanced fertilization: A pathway to sustainable agriculture and food security. Front Microbiol 16: 1536524. https://doi.org/10.3389/fmicb.2025.1536524.
Xiong M, Wu H, Li J. Wan H, Chen X, Liu B, He J. 2025. Terrimonas alba sp. nov., an ochratoxin a degrading strain isolated from Lakeside Soil. Curr Microbiol 82: 269. https://doi.org/10.1007/s00284-025-04254-6.
Xu Y, Li H, Tan L, Li Q, Liu W, Zhang C, Gao Y, Wei X, Gong Q, Zheng X. 2022. What role does organic fertilizer actually play in the fate of antibiotic resistome and pathogenic bacteria in planting soil? J Environ Manag 317: 115382. https://doi.org/10.1016/j.jenvman.2022.115382.
Yadav R, Walia SS, Dheri GS, Singh G, Sharma K. 2024. Balanced fertilization through integrated nutrient management improves soil health, productivity, and profitability in potato-maize (fodder)-maize system under Inceptisols of Indian subtropics. J Soil Sci Plant Nutr 25: 3881-3900. https://doi.org/10.1007/s42729-025-02372-5.
Yin S, Suo F, Zheng Y, You X, Li H, Wang J, Zhang C, Li Y, Cheng Y. 2022. Biochar-compost amendment enhanced sorghum growth and yield by improving soil physicochemical properties and shifting the soil bacterial community in a coastal soil. Front Environ Sci 10: 1036837. https://doi.org/10.3389/fenvs.2022.1036837.
Yu L, Li D, Zhang Y, Wang Y, Yao Q and Yang K. 2024. An optimal combined slow-release nitrogen fertilizer and urea can enhance the decomposition rate of straw and the yield of maize by improving soil bacterial community and structure under full straw returning system. Front Microbiol 15:1358582. https://doi: 10.3389/fmicb.2024.13585.
Zhang Y, Shen H, He X, Thomas BW, Lupwayi NZ, Hao X, Thomas MC, Shi X. 2017. Fertilization shapes bacterial community structure by alteration of soil pH. Front Microbiol 8: 1325. https://doi.org/10.3389/fmicb.2017.01325.
Zhou Z, Wu H, Li D, Zeng W, Huang J, Wu Z. 2022. Comparison of gut microbiome in the Chinese mud snail (Cipangopaludina chinensis) and the invasive golden apple snail (Pomacea canaliculata). PeerJ 5 (10): e13245. https://doi.org/10.7717/peerj.13245.