Characterization and Purification a Specific Xylanase Showing Arabinofuranosidase Activity from Streptomyces spp. 234P-16

ANJA MERYANDINI; TRIO HENDARWIN; FAHRRUROZI FAHRRUROZI; ALINA AKHDIYA; DEDEN SAPRUDIN; YULIN LESTARI

doi:10.13057/biodiv/d100302

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DOI https://doi.org/10.13057/biodiv/d100302

Issue

Vol. 10 No. 3 (2009)

Section

Articles

ANJA MERYANDINI ♥

Biology Department, Faculty of Mathematics and Natural Science, Bogor Agricultural University (IPB), Bogor 16680.

TRIO HENDARWIN

Biology Department, Faculty of Mathematics and Natural Science, Bogor Agricultural University (IPB), Bogor 16680.

FAHRRUROZI FAHRRUROZI

Biology Department, Faculty of Mathematics and Natural Science, Bogor Agricultural University (IPB), Bogor 16680.

ALINA AKHDIYA

Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development (ICABIOGRAD), Bogor 16110

DEDEN SAPRUDIN

Chemistry Department, Faculty of Mathematics and Natural Science, Bogor Agricultural University (IPB), Bogor 16680

YULIN LESTARI

Chemistry Department, Faculty of Mathematics and Natural Science, Bogor Agricultural University (IPB), Bogor 16680

Abstract

Streptomyces spp 234P-16 producing xylanase was isolated from soil sample from Padang, West Sumatra, Indonesia. Crude enzyme (produced by centrifuging the culture at 14000 rpm for about 5 minutes) and purified xylanase have an optimum condition at pH 5 and 90oC. Crude xylanase have half life time of 4 hours, whereas purified xylanase have half life time of 2.5 hours at 90oC. The molecular mass of purified xylanase was determined to be 42.4 kDa. The Arabinofuranosidase have a Km and Vmax value of 1.98 mg/mL and 523 Î¼mol/minute/mg, respectively

Key words: xylanase, xylooligosaccharide, arabinofuranosidase, Streptomyces.

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