Distribution of Staphylococcus haemolyticus as the most dominant species among Staphylococcal infections at the Zainoel Abidin Hospital in Aceh, Indonesia
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Abstract
Abstract. Suhartono S, Hayati Z, Mahmuda Mahmuda M. 2019. Distribution of Staphylococcus haemolyticus as the most dominant species among Staphylococcal infections at the Zainoel Abidin Hospital in Aceh, Indonesia. Biodiversitas 20: 2076-2080. The occurrence of Staphylococcus-related infections is emerging and might potentially harbor multidrug resistance leading to the major risks of hospital-associated infection. The study aimed to determine the dominant species distributed among Staphylococcus-related infections from clinical specimens and determine antibiotic resistance profile of the dominant species. The clinical samples were collected from inpatients and outpatients at the Zainoel Abidin Regional Hospital of Aceh, Indonesia during March 2017-March 2018. All clinical samples were mainly inoculated to plates containing blood agar followed by identification and determination of their antibiotic susceptibility using VITEK® 2 Compact. Statistical analysis was performed using Chi-square test or Fisher's exact test when appropriate to determine the independence of frequency distributions, and the tests were considered statistically significant at a P ? 0.05 on two-tailed. Of 693 Staphylococcus isolates found in the clinical specimens, Staphylococcus haemolyticus was the most predominant isolates with a total of 233 (32.2%), and it was identified with a high prevalence of methicillin-resistance (95.96%) termed as methicillin-resistant Staphylococcus haemolyticus (MRSH). MRSH were found in the blood samples and ICU patients accounting for 64.1% and 55.61%, respectively. The resistance profile of MRSH isolates exhibited a high level of resistance (more than 85%) to a wide range of antibiotics, including beta-lactams, (first to the fourth generation) cephalosporins, fluoroquinolones, carbapenems, and macrolides. This might be an alarming occurrence and require serious action to prevent MRSH dissemination of healthcare infections in the future.
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Chauhan D, Verma S, Verma R, Sharma G. 2017. Emergence of resistance to linezolid in methicillin resistant Staphylococcus haemolyticus reported from the sub Himalayan region of India. Int J Res Med Sci 5(12):5453-5455.
Correa JE, De Paulis A, Predari S, Sordelli DO, Jeric PE. 2008. First report of qacG, qacH and qacJ genes in Staphylococcus haemolyticus human clinical isolates. J Antimicrob Chemother 62(5):956-960.
Czekaj T, Ciszewski M, Szewczyk EM. 2015. Staphylococcus haemolyticus – an emerging threat in the twilight of the antibiotics age. Microbiol 161(11):2061-2068.
Daniel B, Saleem M, Naseer G, Fida AJJoPMS. 2014. Significance of Staphylococcus haemolyticus in hospital acquired infections. J Pioneer Med Sci 4(3):119-125.
Dzen SM, Santoso S, Roekistiningsih R, Santosaningsih D. 2013. Perbedaan pola reistensi Staphylococcus koagulase negative isolate darah terhadap antibiotika di RSU Dr. Saiful Anwar Malang tahun 2000-2001 dengan 2004-2005. J Ked Hewan 21(3):127-132.
Hanberger H, Antonelli M, Holmbom M, Lipman J, Pickkers P, Leone M, Rello J, Sakr Y, Walther SM, Vanhems P. 2014. Infections, antibiotic treatment and mortality in patients admitted to ICUs in countries considered to have high levels of antibiotic resistance compared to those with low levels. BMC Infect Dis 14(1):513
Hitzenbichler F, Simon M, Salzberger B, Hanses FJI. 2017. Clinical significance of coagulase-negative Staphylococci other than S. epidermidis blood stream isolates at a tertiary care hospital. Infection 45(2):179-186.
Hope R, Livermore DM, Brick G, Lillie M, Reynolds R, on behalf of the BWPoRS. 2008. Non-susceptibility trends among Staphylococci from bacteraemias in the UK and Ireland, 2001–06. J Antimicrob Chemother 62: ii65-ii74.
Hosseinkhani F, Tammes Buirs M, Jabalameli F, Emaneini M, van Leeuwen WB. 2018. High diversity in SCCmec elements among multidrug-resistant Staphylococcus haemolyticus strains originating from paediatric patients; characterization of a new composite island. J Med Microbiol 67(7):915-921.
Kim JS, Kim HS, Park JY, Koo HS, Choi CS, Song W, et al. 2012. Contamination of X-ray cassettes with methicillin-resistant Staphylococcus aureus and methicillin-resistant Staphylococcus haemolyticus in a Radiology Department. Ann Lab Med 32(3): 206-209.
Kristóf K, Kocsis E, Szabó D, Kardos S, Cser V, Nagy K, et al. 2007. Significance of methicillin–teicoplanin resistant Staphylococcus haemolyticus in bloodstream infections in patients of the Semmelweis University hospitals in Hungary. Eur J Clin Microbiol Infect Dis 2011;30(5):691-9.
Lloyd DH. Reservoirs of antimicrobial resistance in pet animals. Clin Infect Dis 45: S148-S52.
Luiza P, Ivo BC, Cataneli PV, Adilson O, Rocha BA, Henrique CC, et al. 2016. Susceptibility profile of Staphylococcus epidermidis and Staphylococcus haemolyticus isolated from blood cultures to vancomycin and novel antimicrobial drugs over a period of 12 Years. Microb Drug Resist 22(4):283-93.
Matlani M, Shende T, Bhandari V, Dawar R, Sardana R, Gaind R. 2016. Linezolid-resistant mucoid Staphylococcus haemolyticus from a tertiary-care centre in Delhi. New Microbes New Infect11:57-58.
Monsen T, Karlsson C, Wistrom J. 2005. Spread of clones of multidrug resistant, coagulase negative Staphylococci within a university hospital. Infect Control Hosp Epidemiol 26(1):76-80.
Pereira PMA, Binatti VB, Sued BPR, Ramos JN, Peixoto RS, Simões C, et al. 2014. Staphylococcus haemolyticus disseminated among neonates with bacteremia in a neonatal intensive care unit in Rio de Janeiro, Brazil. Diagn Microbiol Infect Dis 78(1):85-92.
Rodríguez-Aranda A, Daskalaki M, Villar J, Sanz F, Otero JR, Chaves F. Nosocomial spread of linezolid-resistant Staphylococcus haemolyticus infections in an intensive care unit. Diagn Microbiol Infect Dis 63(4):398-402.
Rogers KL, Fey PD, Rupp ME. 2009. Coagulase-negative Staphylococcal infections. Infect Dis Clin North Ame 23(1): 73-98.
Ruzauskas M, Siugzdiniene R, Klimiene I, Virgailis M, Mockeliunas R, Vaskeviciute L, et al. 2014. Prevalence of methicillin-resistant Staphylococcus haemolyticus in companion animals: a cross-sectional study. Ann Clin Microbiol Antimicrob 13(1):56.
Shinefield HR, Ruff NL. 2009. Staphylococcal infections: A historical perspective. Infect Dise Clin North Ame 23(1): 1-5.
Sidhu MS, Oppegaard H, Devor TP, Sørum H. 2007. Persistence of multidrug-resistant Staphylococcus haemolyticus in an animal veterinary teaching hospital clinic. Microb Drug Resist 13(4):271-280.
Squeri R, Grillo OC, La Fauci V. 2012. Surveillance and evidence of contamination in hospital environment from meticillin and vancomycin-resistant microbial agents. J Prev Med Hyg 53(3):143-145.
Takeuchi F, Watanabe S, Baba T, Yuzawa H, Ito T, Morimoto Y, et al. 2005. Whole-genome sequencing of Staphylococcus haemolyticus uncovers the extreme plasticity of its genome and the evolution of human-colonizing Staphylococcal Species. J Bacteriol 187(21):7292-7308.
Teeraputon S, Santanirand P, Wongchai T, Songjang W, Lapsomthob N, Jaikrasun D, et al. 2017. Prevalence of methicillin resistance and macrolide–lincosamide–streptogramin B resistance in Staphylococcus haemolyticus among clinical strains at a tertiary-care hospital in Thailand. New Microbes New Infect 19:28-33.
Ternes YM, Lamaro-Cardoso J, André MCP, Pessoa VP, Vieira MAdS, Minamisava R, et al. 2013. Molecular epidemiology of coagulase-negative Staphylococcus carriage in neonates admitted to an intensive care unit in Brazil. BMC Infect Dis 13:572.
Veach LA, Pfaller MA, Barrett M, Koontz FP, Wenzel RP. 1990. Vancomycin resistance in Staphylococcus haemolyticus causing colonization and bloodstream infection. J Clin Microbiol. 28(9):2064-2068.
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