Identification and expression of gonadotrophin hormones in gouramy (Osphronemous gouramy, Lacepède, 1801) under photoperiod manipulations
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Abstract
Abstract. Prayogo NA, Siregar AS, Sukardi P, Bessho Y. 2020. Identification and expression of gonadotrophin hormones in gouramy (Osphronemoous gouramy, Lacepède, 1801) under photoperiod manipulations. Biodiversitas 21: 1365-1372. The environment influences fish reproduction. One of the influential environmental factors is photoperiod that regulates the production of hormones for gonad growth and development, gametogenesis, then the reproductive cycle. This research was conducted to determine the effect of photoperiod on the reproductive performance of gouramy with different photoperiod treatments. The design of this study was four photoperiod treatments consisting of 14L: 10D, 16L: 8D and 18L: 6D and controls. Fish samples were divided into 4 aquariums consisting of nine fish and repetition was conducted on each. Fish were kept under photoperiod treatment for 16 weeks. The observed variable was pituitary activity. Pituitary activity was evaluated by measuring the expression of FSH and LH genes using Real Time PCR. The results showed that gouramy had FSH and LH genes with 483 bp and 506 bp. FSH and LH gene expression increased with long photoperiod exposure (P <0.05). These findings indicate that photoperiods regulate the reproduction of gouramy through the HPG axis and control the production of the hormone Gonadotropin.
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Martins RS, Gomez A, Zanuy S, Carrillo M, Canário AV. 2015. Photoperiodic Modulation of Circadian Clock and Reproductive Axis Gene Expression in the Pre-Pubertal European Sea Bass Brain. PLoS One. 2015;10(12):e0144158.
Minniti F, Maisano M, Giannito A, Mauceri A, Sasuolo F. 2007. Gth i and gth ii in the pituitary gland of swordfish (xiphias gladius l.). Italian journal of zoology, 76:269-278.
Miranda LA, Strussmann CA, Somoza GM. 2008. Effects of light and temperature conditions on the expression of gnrh and gth genes and levels of plasma steroids in odontesthes bonariensis females. Fish physiology biochemical 35:101–108
Prayogo NA, Wijayanti GE, Murwantoko, Kawaichi M, Astuti P. 2012. Effect of photoperiods on melatonin levels, estradiols level and the expression of cgnrh-ii and sgnrh genes, in hard-lipped barb (osteochilus hasselti c.v.). Journal of global veterinaria, 8(6) : 591-597.
Prayogo NA, Wijayanti GE, Sulistyo I, Sukardi P. 2016a. Cloning and expression cgnrh-ii and sgnrh genes in hard-lipped barb (osteochilus hasselti c.v.). Biodiversitas, biodiversitas. 17(29) : 523-530
Prayogo NA, Siregar AS, Sukardi P. 2016b. The disruptive effect mercurychloride (hgcl) on gene expression of cgnrh-ii sgnrh, and estradiol level in silver sharkminnow (osteochillus hasseltii c.v.). Turkish journal fishery and aquatic science. 16(2) : 1003-1009
Prayogo NA, Siregar AS, Sukardi P, Bessho Y. 2018. Molecular Cloning Of The Vitellogenin Gene In The Hard-Lipped Barb (Osteochillus Hasseltii C.V) And Photoperiod’s Effects On Gene Expression. Biotropia journal. 25(3) : 211-233.
Qingbo T, Mazur M, and Mellon PL. 2005. The protein kinase c pathway acts through multiple transcription factors to repress gonadotropin-releasing hormone gene expression in hypothalamic gt1–7 neuronal cells. Molecular endocrinology 19(11): 2769–2779
Skjæraasen JE, Nilsen T, Kjesbu OS. 2006. Timing and determination of potential fecundity in atlantic cod (gadus morhua). Fish aquatic science. 63: 310–320.
Siregar, A.S., Prayogo, N.A. 2018. The disruptive effect of mercury chloride (HgCl) on gene expression of gonadotrophin hormones and testosterone level in male silver sharkminnow (Osteochilus hasseltii C.V.) (Teleostei: Cyprinidae). European Zoological Journal 84(1), pp. 436-443
Ulloa AA, Timossi C, Damian MP. 1999. Role of glycosylation in function of follicle-stimulating hormone. Endocrine. 11(3): 205–15.
Veras GC, Murgas LDS, Rosa PV, Zangeronimo MG, Ferreira MSS, Leon JAS. 2013. Effect of photoperiod on locomotor activity, growth, feed efficiency and gonadal development of Nile tilapia. Revista Brasileira de Zootecnia, 42(12), 844-849
Wang Q, Sham KW, Ogawa S, Li S, Parhar IS, Cheng CH, Liu X, and Lin H. 2013. Regulation of the two kiss promoters in goldfish (carassius auratus) by estrogen via different eralpha pathways journal mol. Cell. Endocrinol.
Xiong F, and Hew CL. 1991. Chinook salmon gonadotropin ii ß-subunit gene encodes multiple messenger ribonucleic acids. Canadian Journal Zoology. 69: 2572–2578.
Yu KL and Peter RE. 1992. Adrenergic and dopaminergic regulation of gonadotropin-releasing hormone release from goldfish preoptic–anterior hypothalamus and pituitary in vitro. General and comparative endocrinology 85 138–146
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