Developmental, morphological and molecular variation of commercial Ganoderma spp. accessions from southern Vietnam
##plugins.themes.bootstrap3.article.sidebar##
##plugins.themes.bootstrap3.article.main##
Abstract
Abstract. Ho VT, Vo TNH, Le NG. 2019. Developmental, morphological and molecular variation of commercial Ganoderma spp. accessions from southern Vietnam. Biodiversitas 20: 3684-3689. In Vietnam, the market value of Ganoderma spp. increases markedly recently due to the high demand as an effective medicine. However, the breeding programs for this mushroom are still less effective due to the poor knowledge of genetic composition. This study was conducted to investigate the diversity of some commercial Ganoderma spp. accessions from southern Vietnam using both traditional techniques using developmental and morphological characterization, and molecular method using ISSR markers. A total of nine Ganoderma spp. samples were collected from production areas and research institutes in southern provinces of Vietnam and targeted for developmental and molecular characterization. Developmental and morphological variations among accessions were evaluation. In addition, 20 ISSR markers were also utilized for further understanding genetic composition of these mushrooms. The obtained measurement data show a large variation among the studied Ganoderma spp. accessions in term of mycelia growth, diameter, shape, and dried weight of fruiting bodies. Molecular data from 20 ISSR markers also supported genetic variation of the examined samples. Based on the results of this study, the samples of Ganoderma spp. accessions from southern Vietnam can be divided into two main groups which are not correlated to geographical locations of sample collection sites. The results are potential for classification and provide new insights for molecular assisted selection of Ganoderma spp.
##plugins.themes.bootstrap3.article.details##
Botstein D, White RL, Skolnick M, Davis RW. 1980. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet. 32(3): 314-331. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1686077/
Du J, Guo HB, Li Q, Forsythe A, Chen XH, Yu XD. 2018. Genetic diversity of Lepista nuda (Agaricales, Basidiomycota) in Northeast China as indicated by SRAP and ISSR markers. PLoS ONE 13(8): e0202761. https://doi.org/10.1371/journal.pone.0202761
Hong SG, Jung HS. 2004. Phylogenetic analysis of Ganoderma based on nearly complete mitochondrial small-subunit ribosomal DNA sequences. Mycologia. 96(4): 742 – 755. https://doi.org/10.2307/3762108
Iqbal A, Sadia B, Khan AI, Awan FS, Kainth RA, Sadaqat HA. 2010. Biodiversity in the sorghum (Sorghum bicolor L. Moench) germplasm of Pakistan. Genet Mol Res. 9: 756-764. https://doi.org/10.4238/vol9-2gmr741
Jaipreet KR, Mohamamd A, Uma SS. 2015. Relative efficiency of RAPD and ISSR markers in assessment of DNA polymorphism and genetic diversity among Pseudomonas strains. Afr J Biotechnol. 14(13): 1097-1106. https://doi.org/10.5897/ajb10.1951
Jing Y, Peng M, yang L, Wang Q. 2017. Evaluation of genetic diversity among Piptoporus betulinus as revealed by inter simple sequence repeat markers. Biotechnol Biotec Eq. 31(2): 333-338. https://doi.org/10.1080/13102818.2016.1276413
Le DHV, Tran DH. 2008. Biological characteristics and yield of some trains of lingshi mushroom (Ganoderma lucidum) in Thue Thien Hue province. Hue University Journal of Science 49: 209-216. (In Vietnamese). http://www.hueuni.edu.vn/portal/data/doc/tapchi/49_24.pdf
Levi A, Thomas CE, Newman M, Reddy OUK, Zhang X, Xu Y. 2004. ISSR and AFLP markers differ among American watermelon cultivars with limited genetic diversity. J Am Soc Hortic Sci. 129(4): 553-558. https://doi.org/10.21273/jashs.129.4.0553
Liu SR, Ke BR, Zhang WR, Liu XR. Wu XP. 2017. Breeding of new Ganoderma lucidum strains simultaneously rich in polysaccharides and triterpenes by mating basidiospore-derived monokaryons of two commercial cultivars. Sci Hortic. 216: 58-65. https://doi.org/10.1016/j.scienta.2016.12.016
Patel P, Rajkumar BK, Parmar P, Shah R, Krishnamurthy R. 2018. Assessment of genetic diversity in Colletotrichum falcatum Went accessions based on RAPD and ISSR markers. J Genet Eng Biotechnol. 16(1): 153–159. https://doi.org/10.1016/j.jgeb.2017.11.006
Porebski S, Bailey LG, Baum BR. 1997. Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components. Plant Mol Biol Rep. 15(1): 8-15. https://doi.org/10.1007/bf02772108
Rohlf F. 1987. NTSYS-pc: Microcomputer Programs for Numerical Taxonomy and Multivariate Analysis. Am stat. 41(4): p330. https://doi.org/10.2307/2684761
Singh A, Behera T, Chandel D, Sharma P, Singh N. 2015. Assessing genetic relationships among bitter gourd (Momordica charantia L.) accessions using inter-simple sequence repeat (ISSR) markers. J Hort Sci Biotechnol. 82: 217-222. https://doi.org/10.1080/14620316.2007.11512222
Staniaszek M, Marczewski W, Szudyga K, Maszkiewicz J, Czaplicki A, Qian G. 2002. Genetic relationship between Polish and Chinese strains of the mushroom Agaricus bisporus (Lange) Sing determined by the RAPD method. J Appl Genet. 43: 43-47. http://jag.igr.poznan.pl/2002-Volume-43/1/pdf/2002_Volume_43_1-43-47.pdf
Wolfe AD, Liston A. 1998. Contributions of PCR-based methods to plant systematics and evolutionary biology. In: P. S. Soltis PS, Soltis DE, Doyle JJ (eds), Molecular systematics of plants: DNA sequencing. Kluwer. New York, USA. https://doi.org/10.1007/978-1-4615-5419-6_2
Most read articles by the same author(s)
- VIET THE HO, MINH PHUONG NGUYEN, An in silico approach for evaluation of rbcL and matK loci for DNA barcoding of Cucurbitaceae family , Biodiversitas Journal of Biological Diversity: Vol. 21 No. 8 (2020)
- VIET THE HO, QUOC NGUYEN NGO, Short Communication: Using RAPD technique to evaluate genetic diversity of longan (Dimocarpus longan) population in Vietnam , Biodiversitas Journal of Biological Diversity: Vol. 18 No. 4 (2017)
- THI KIM PHUONG TRAN, MINH HAC PHAM, THI HUONG TRINH, SASANTI WIDIARSIH, VIET THE HO, Investigation of the genetic diversity of jewel orchid in Vietnam using RAPD and ISSR markers , Biodiversitas Journal of Biological Diversity: Vol. 23 No. 9 (2022)