Short Communication: Identification of Leptin gene in crossbred beef cattle
##plugins.themes.bootstrap3.article.sidebar##
Issue
Section
Articles
##plugins.themes.bootstrap3.article.main##
Abstract
Abstract. Anugratama LE, Hartatik T. 2020. Short Communication: Identification of Leptin gene in crossbred beef cattle. Biodiversitas 21: 226-230. Leptin is a gene that affects animal weight. Leptin gene is known to control body weight, feed intake, energy expenditure, immune function, and reproduction. This study aims to identify the diversity of the Leptin gene in crossbred beef cattle, Sumba Ongole cattle, Brahman cross cattle, Bali cattle, buffalo, sheep, and goat by comparing with four GenBank data of cattle. Crossbred beef cattle obtained from Klaten, Central Java, Indonesia. Leptin nucleotide sequences were analyzed using BioEdit to identify Single Nucleotide Polymorphism (SNP). To create amino acid change in Leptin gene, the coding sequence of exon 2 was established using BioEdit ver. 7.0.5. Phylogenetic tree and genetic distance have been analyzed based on the Leptin gene using MEGA 10.1.1 program. The result shows that eight variations of SNP were found in exon 2. The phylogenetic tree represents that crossbreed beef cattle, Sumba Ongole cattle, Brahman cross cattle, Bali cattle, Bos taurus, Bos indicus, Bos frontalis, Bos grunniens, Bubalus bubalis are in the same cluster with various genetic distance. The results of this study are expected to provide genetic information that will be used for further research on the relationship between Leptin gene polymorphisms to animal weight.
##plugins.themes.bootstrap3.article.details##
References
Barzehkar R, Salehi A, Mahjoubi F. 2009. Polymorphism of the ovine leptin gene and its association with growth and carcass traits in three Iranian sheep breeds. Iran J Biotechnol. 7: 241-246.
Buchanan FC, Fitzsimmons CJ, Van-Kessel AG, Thue TD, Winkelman-Sim DC, Schmutz SM. 2002. Association of a missense mutation in the bovine leptin gene with carcass fat content and leptin mRNA levels. Genet Sel Evol. 34: 105-116.
Chung ER, Shin SC, Shin KH, Chung KY. 2008. SNP discovery in leptin promoter gene and association with meat quality and carcass traits in Korean cattle. Asian Aust JAnim Sci. 21: 1689-1695.
Fathoni A, Maharani D, Aji RN, Choiri R, Sumadi S. 2018. Polymorphism of the SNP g. 1180 C>T in leptin gene and its association with growth traits and linear body measurement in kebumen ongole grade cattle. Journal of the Indonesian Tropical Animal Agriculture. 44: 125-134.
Fruhbeck G, Jebb SA, Prentice AM. 1998. Leptin: Physiol. Pathiphysiol. J. Clin. Physiol. 18: 399-419.
Hernandez N, JCM. Gonzalez, GMP Bracamonte, AMS Rincon, NL Villalobos, ST Morris, FB Encinia, EO Rivas, VIP. Contreras, and LAM Garcia. 2016. Association of polymorphisms in growth hormone and leptin candidate genes with live weight traits of Brahman cattle. Genet. Mol. Res. 15: 1-9.
Javanmard, A., N. Asadzadeh and F. Sarhadi. 2010. DNA polymorphism of bovine pituitary-specific transcription factor and leptin gene between Iranian Bosindicus and Bos taurus cattle. Am. J. Agric. Biol. Sci. 5(3):282-285.
Kimura, M. 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J. Mol. Evol. 16: 111-120
Konfortov BA, Licence VE, Miller JR. 1999. Re-sequencing dna from a diverse panel of cattle reveals a high level of polymorphism in both intron and exon, maam. Genome 10. 1142-1145
Nkrumah JD, Li C, Yu J, Hansen C, Keisler DH, Moore SS. 2005. Polymorphisms in the bovine leptin promoter associated with serum leptin concentration, growth, feed intake, feeding behavior, and measures of carcass merit. J Anim Sci. 83: 20-28.
Orrù L., GF Cifuni, E Piasentier and M Corazzin. 2011. Association analyses of single nucleotide polymorphisms in the LEP and SCD1 genes on the fatty acid profile ofmuscle fat in Simmental bulls. Meat Sci. 87(4): 344-348.
Raza, K. 2012. Application of data mining in bioinformatics. Indian Journal of Computer Science and Engineering. 1: 114-118.
Schenkel, FS, SP Miller, X Ye, SS More, JD Nkrumah, C Li, J Yu, IB Mandell, JW Wilton and JL Williams. 2005. Association of single nucleotide polymorphisms in the leptin gene with carcass and meat quality traits of beef cattle. J. Anim. Sci. 83(9): 2009-2020.
Silva DBS, BA Crispim, LE Silva, JA Oliveira, F Siqueira, LO Seno and AB Grisolia. 2014. Genetic variations in theleptin gene associated with growth andcarcass traits in Nellore cattle. Genet. Mol.Res. 13(2): 3002-3012.
Yang D, Chen H, Wang X, Tian Z, Tang L, Zhang Z, Lei C, Zhang L, Wang Y. 2007. Association of polymorphisms of leptin gene with body weight and body sizes indexes in Chinese indigenous cattle. J Genet Genomics. 34(5): 400-5.
Buchanan FC, Fitzsimmons CJ, Van-Kessel AG, Thue TD, Winkelman-Sim DC, Schmutz SM. 2002. Association of a missense mutation in the bovine leptin gene with carcass fat content and leptin mRNA levels. Genet Sel Evol. 34: 105-116.
Chung ER, Shin SC, Shin KH, Chung KY. 2008. SNP discovery in leptin promoter gene and association with meat quality and carcass traits in Korean cattle. Asian Aust JAnim Sci. 21: 1689-1695.
Fathoni A, Maharani D, Aji RN, Choiri R, Sumadi S. 2018. Polymorphism of the SNP g. 1180 C>T in leptin gene and its association with growth traits and linear body measurement in kebumen ongole grade cattle. Journal of the Indonesian Tropical Animal Agriculture. 44: 125-134.
Fruhbeck G, Jebb SA, Prentice AM. 1998. Leptin: Physiol. Pathiphysiol. J. Clin. Physiol. 18: 399-419.
Hernandez N, JCM. Gonzalez, GMP Bracamonte, AMS Rincon, NL Villalobos, ST Morris, FB Encinia, EO Rivas, VIP. Contreras, and LAM Garcia. 2016. Association of polymorphisms in growth hormone and leptin candidate genes with live weight traits of Brahman cattle. Genet. Mol. Res. 15: 1-9.
Javanmard, A., N. Asadzadeh and F. Sarhadi. 2010. DNA polymorphism of bovine pituitary-specific transcription factor and leptin gene between Iranian Bosindicus and Bos taurus cattle. Am. J. Agric. Biol. Sci. 5(3):282-285.
Kimura, M. 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J. Mol. Evol. 16: 111-120
Konfortov BA, Licence VE, Miller JR. 1999. Re-sequencing dna from a diverse panel of cattle reveals a high level of polymorphism in both intron and exon, maam. Genome 10. 1142-1145
Nkrumah JD, Li C, Yu J, Hansen C, Keisler DH, Moore SS. 2005. Polymorphisms in the bovine leptin promoter associated with serum leptin concentration, growth, feed intake, feeding behavior, and measures of carcass merit. J Anim Sci. 83: 20-28.
Orrù L., GF Cifuni, E Piasentier and M Corazzin. 2011. Association analyses of single nucleotide polymorphisms in the LEP and SCD1 genes on the fatty acid profile ofmuscle fat in Simmental bulls. Meat Sci. 87(4): 344-348.
Raza, K. 2012. Application of data mining in bioinformatics. Indian Journal of Computer Science and Engineering. 1: 114-118.
Schenkel, FS, SP Miller, X Ye, SS More, JD Nkrumah, C Li, J Yu, IB Mandell, JW Wilton and JL Williams. 2005. Association of single nucleotide polymorphisms in the leptin gene with carcass and meat quality traits of beef cattle. J. Anim. Sci. 83(9): 2009-2020.
Silva DBS, BA Crispim, LE Silva, JA Oliveira, F Siqueira, LO Seno and AB Grisolia. 2014. Genetic variations in theleptin gene associated with growth andcarcass traits in Nellore cattle. Genet. Mol.Res. 13(2): 3002-3012.
Yang D, Chen H, Wang X, Tian Z, Tang L, Zhang Z, Lei C, Zhang L, Wang Y. 2007. Association of polymorphisms of leptin gene with body weight and body sizes indexes in Chinese indigenous cattle. J Genet Genomics. 34(5): 400-5.
Most read articles by the same author(s)
- TETY HARTATIK, AHMAD FATHONI, SIGIT BINTARA, ISMAYA, PANJONO, B.P. WIDYOBROTO, ALI AGUS, IGS. BUDISATRIA, PASCAL LEROY, Short communication: The genotype of growth hormone gene that affects the birth weight and average daily gain in crossbred beef cattle , Biodiversitas Journal of Biological Diversity: Vol. 21 No. 3 (2020)
- DINO EKA PUTRA, SUMADI SUMADI, TAKUYA KANAZAWA, TETY HARTATIK, Short Communication: Identification of Growth Hormone gene polymorphism for beef cattle in Pesisir Selatan District, West Sumatra, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 17 No. 2 (2016)
- DWI AHMAD PRIYADI, PANJONO PANJONO, SIGIT BINTARA, TETY HARTATIK, Genotype of Brahman and Brahman Cross Cattle based on SNP in Insulin-Like Growth Factor Binding Protein-3 (IGFBP-3) gene sequences , Biodiversitas Journal of Biological Diversity: Vol. 18 No. 2 (2017)
- TETY HARTATIK, BAYU DEWANTORO PUTRA SOEWANDI, SLAMET DIAH VOLKANDARI, ARNOLD CHRISTIAN TABUN, SUMADI SUMADI, WIDODO WIDODO, Short Communication: Genetic identification of local pigs, and imported pigs Duroc) based on cytochrome b sequence analysis , Biodiversitas Journal of Biological Diversity: Vol. 17 No. 1 (2016)
- TETY HARTATIK, DWI NUR HAPPY HARIYONO, YUDI ADINATA, Short Communication: Genetic diversity and phylogenetic analysis of two Indonesian local cattle breeds based on cytochrome b gene sequences , Biodiversitas Journal of Biological Diversity: Vol. 20 No. 1 (2019)
- WIDYA PINTAKA BAYU PUTRA, TETY HARTATIK, SUMADI SUMADI, Growth hormone genotyping by MspI restriction enzyme and PCRRFLP method in Aceh cattle breed at Indrapuri District, Aceh Province, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 15 No. 1 (2014)
- YUSNI KHAIRANI TAMPUBOLON, ALI AGUS, TETY HARTATIK, SIGIT BINTARA, ISMAYA, BUDI PRASETYO WIDYOBROTO, I GEDE SUPARTA BUDISATRIA, PASCAL LEROY, NICOLAS ANTOINE-MOUSSIAUX, PANJONO, Reproductive performance of Belgian Blue and Wagyu cross cows , Biodiversitas Journal of Biological Diversity: Vol. 24 No. 10 (2023)
- DEVI ERMAWATI, PANJONO, SIGIT BINTARA, TETY HARTATIK, Diversity of partial sequence leptin gene (Exon 3) in crossbred cattle compared to GenBank database , Biodiversitas Journal of Biological Diversity: Vol. 23 No. 11 (2022)