Short Communication: Polymorphism at third exon of the Myostatin gene and its association with growth and carcass traits in Batur sheep
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Abstract. Haren HIH, Purwantini D, Sumaryadi MY, Prayitno. 2020. Short Communication: Polymorphism at third exon of the Myostatin gene and its association with growth and carcass traits in Batur sheep. Biodiversitas 21: 2074-2078. The present study aimed to investigate the association between myostatin (MSTN) genotype and two traits, growth and carcass, in Batur sheep. Blood samples representing thirty head were collected and genomic DNA was extracted. A specific primer designed to amplify the MSTN gene, samples sequenced then, used the BioEdit program to identify any mutation. Calculation of genotypes, gene and allele frequencies, heterozygosities, and Chi-square test was performed. Only two alleles observed (G and C) resulted in three genotypes. 11 polymorphic sites were observed, transversion at c.*121G>C, and one individual G>A which disrupted reading frame of whole MSTN sequenced, genotypic and allelic frequencies were 0.552 GG, 0.379 GC, and 0.069 CC, where the allele frequency was 0.741 G and 0.259 C. Lambs that carrying genotype GC had slightly more pre-slaughter weight, hot carcass weight, cold carcass weight, carcass length, and total yield (lion, shoulder, leg yield, and thigh) compared to those carrying genotype GG. There was no significant effect of the MSTN genotype on carcass traits (p=0.05). Polymorphic site c.*121 G>C is present in Batur sheep for the first time about the association with the MSTN gene however, it has not effects weaning weight, 6-month weight, and studied carcass traits.
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References
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Boman I, and Våge D. 2009. An insertion in the coding region of the Myostatin (MSTN) gene affects carcass conformation and fatness in the Norwegian Spælsau (Ovis aries). BMC Research Notes, 2(1), p.98, http:// doi: 10.1186/1756-0500-2-98
Boman I, Klemetsdal G, Nafstad O, Blichfeldt T, and Våge D. 2010. Impact of two Myostatin (MSTN) mutations on weight gain and lamb carcass classification in Norwegian White Sheep (Ovis aries). Genetics Selection Evolution, 42(1), http:// doi: 10.1186/1297-9686-42-4
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Dunner S, Miranda M E, Amigues Y, Canón J, Georges M, Hanset R, Williams J L, Ménissier F. 2003. Haplotype diversity of the Myostatin gene among beef cattle breeds. Genetic Selection Evolution. 35, 103–118, http:// doi: 10.1186/1297-9686-35-1-103
Grobet L, Martin L J R, Poncelet D, Pirottin D, Brouwers B, Riquet J, Schoeberlein A, Dunner S, Menissier F, Massabanda J Fries R, Hanset R, Georges M. 1997. A deletion in the bovine Myostatin gene causes the double-muscled phenotype in cattle. Nature Genetics, 17, 71–74. http:// doi: 10.1038/ng0997-71
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McPherron A C, Lawler A M, Lee S J. 1997. Regulation of skeletal muscle mass in mice by a new TGF- superfamily member. Nature 387, 83–90, http:// doi: 10.1038/387083a0
Mosher D S, Quignon P, Bustamante C D, Sutter N B, Mellersh C S, Parker H G, Ostrander E A. 2007. A mutation in the Myostatin gene increases muscle mass and enhances racing performance in heterozygote dogs. PLoS Genetics, 3(5), 779–786, http:// doi: 10.1371/journal.pgen.0030079
Nei Mn and kumar S. 2000. Molecular evaluation and phylogenetics. Oxford Univ Press, New York,
Prayitno. 2010. Analisis Genetik dan Kekerabatan Domba Batur dengan Domba Lokal dan Merino Menggunakan Marker RAP-DNA. Laporan Penelitian Hibah Doktor. Universitas Gadjah Mada, Yogyakarta
Sahu A, Jeichitra V, Rajendran R, Raja A. 2017. Polymorphism in exon 3 of Myostatin (MSTN) gene and its association with growth traits in Indian sheep breeds. Small Ruminant Research, 149, pp.81-84, http:// doi: 10.1016/j.smallrumres.2017.01.009
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Schuelke M, Wagner K R, Stolz L E, Hübner C, Riebel T, Kömen W, Lee S J. 2004. Myostatin mutation associated with gross muscle hypertrophy in a child. New England Journal of Medicine, 350(26), 2682–2688, http:// doi:10.1056/nejm200409023511018
Sumantri C R, Diyono A, Farajallah, I Inounu. 2008. Polymorphism of calpastatin gene and its effect on body weight of local sheep’s. JITV. 13:117-126.
Zhou H, Hickford J G H, Fang Q. 2008. Variation in the coding region of the Myostatin (GDF8) gene in sheep. Molecular and Cellular Probes, 22, 67- 68, http:// doi: 10.1016/j.mcp.2007.08.004
Boman I, and Våge D. 2009. An insertion in the coding region of the Myostatin (MSTN) gene affects carcass conformation and fatness in the Norwegian Spælsau (Ovis aries). BMC Research Notes, 2(1), p.98, http:// doi: 10.1186/1756-0500-2-98
Boman I, Klemetsdal G, Nafstad O, Blichfeldt T, and Våge D. 2010. Impact of two Myostatin (MSTN) mutations on weight gain and lamb carcass classification in Norwegian White Sheep (Ovis aries). Genetics Selection Evolution, 42(1), http:// doi: 10.1186/1297-9686-42-4
Ciappesoni G, San Julián R, Navajas E, Gimeno D, Gutierrez-Zamit E, Goldberg V, and Brito G. 2014. Genetic evaluation of the Texel breed in Uruguay, Carcass quality traits. 60th International Congress of Meat Science and Technology, Punta del Este, Uruguay
Dehnavi E, Azari M A, Hasani S, Nassiry M R, Mohajer M, Ahmadi A K, Shahmohamadi L, Yousefi S. 2012. Polymorphism of Myostatin gene in Intron 1 and 2 and Exon 3, and their associations with yearling weight, using PCR-RFLP and PCR-SSCP techniques in Zel sheep. Biotechnolgy Research International, http://dx.doi.org/10.1155/2012/472307
De Paula N F, Tedeschi L O, Paulino M F, Fernandes H J, Fonseca M A. 2013. Predicting carcass and body fat composition using biometric measurements of grazing beef cattle. Journal of Animal Science. 91, 3341-3351, http://doi: 10.2527/jas.2012-5233
Dunner S, Miranda M E, Amigues Y, Canón J, Georges M, Hanset R, Williams J L, Ménissier F. 2003. Haplotype diversity of the Myostatin gene among beef cattle breeds. Genetic Selection Evolution. 35, 103–118, http:// doi: 10.1186/1297-9686-35-1-103
Grobet L, Martin L J R, Poncelet D, Pirottin D, Brouwers B, Riquet J, Schoeberlein A, Dunner S, Menissier F, Massabanda J Fries R, Hanset R, Georges M. 1997. A deletion in the bovine Myostatin gene causes the double-muscled phenotype in cattle. Nature Genetics, 17, 71–74. http:// doi: 10.1038/ng0997-71
Hadjipavlou G, Matika O, Clop A, and Bishop S. 2008. Two single nucleotide polymorphisms in the Myostatin (GDF8) gene have significant association with muscle depth of commercial Charollais sheep. Animal Genetics, 39(4), pp.346-353. http:// doi: 10.1111/j.1365-2052.2008.01734.x
Han J, Forrest R H, Hickford J G H. 2013. Genetic variations in the Myostatin gene (MSTN) in New Zealandsheep breeds. Molecular Biology Reports, 40(11), 6379–6384, http:// doi: 10.1007/s11033-013-2752-7
Han J, Forrest R H, Sedcole J R, Hickford J G H. 2015. Myostatin (MSTN) gene haplotypes and their association with growth and carcass traits in New Zealand Romney lambs. Small Ruminant Research, 127, 8-19, https://doi.org/10.1016/j.smallrumres.2015.03.015
Hickford J G H, Forrest R H, Zhou H, Fang Q, Han J, Frampton C M, Horrell A L. 2010. Polymorphisms in the ovine Myostatin gene (MSTN) and their association with growth and carcass traits in New Zealand Romney sheep. Animal Genetics. 41, 64–72, http:// doi: 10.1111/j.1365-2052.2009.01965.x
Johnson P L, Dodds K, G, Bain W E, Greer G J, McLean N J, McLaren R J, Galloway S M, van Stijn T C, McEwan J C. 2009. Investigations into the GDF8 g+6273G-A polymorphism in New Zealand Texel sheep. Journal of Animal Science. 87, 1856–1864, http:// doi: 10.2527/jas.2008-1508
Joulia-Ekaza D, Cabello G. 2007. The Myostatin gene physiology and pharmacological relevance. Current opinion in pharmacology, 7(3), 310-315, https://doi.org/10.1016/j.coph.2006.11.011
Knight M I, Daetwyler H D, Hayes B J, Hayden M J, Ball A J, Pethick D W, McDonagh M B. 2014. An independent validation association study of carcass quality, shear force, intramuscular fat percentage and omega-3 polyunsaturated fatty acid content with gene markers in Australian lamb. Meat Science, 96, 1025-1033, http:// doi: 10.1016/j.meatsci.2013.07.008
McPherron A C, Lawler A M, Lee S J. 1997. Regulation of skeletal muscle mass in mice by a new TGF- superfamily member. Nature 387, 83–90, http:// doi: 10.1038/387083a0
Mosher D S, Quignon P, Bustamante C D, Sutter N B, Mellersh C S, Parker H G, Ostrander E A. 2007. A mutation in the Myostatin gene increases muscle mass and enhances racing performance in heterozygote dogs. PLoS Genetics, 3(5), 779–786, http:// doi: 10.1371/journal.pgen.0030079
Nei Mn and kumar S. 2000. Molecular evaluation and phylogenetics. Oxford Univ Press, New York,
Prayitno. 2010. Analisis Genetik dan Kekerabatan Domba Batur dengan Domba Lokal dan Merino Menggunakan Marker RAP-DNA. Laporan Penelitian Hibah Doktor. Universitas Gadjah Mada, Yogyakarta
Sahu A, Jeichitra V, Rajendran R, Raja A. 2017. Polymorphism in exon 3 of Myostatin (MSTN) gene and its association with growth traits in Indian sheep breeds. Small Ruminant Research, 149, pp.81-84, http:// doi: 10.1016/j.smallrumres.2017.01.009
Scholz A M, Bünger L, Kongsro J, Baulain U, Mitchell A D. 2015. Non-invasive methods for the determination of body and carcass composition in livestock: dual-energy X-ray absorptiometry, computed tomography, magnetic resonance imaging, and ultrasound: invited review. Animal 9, 1250–1264, http:// doi: 10.1017/s1751731115000336
Schuelke M, Wagner K R, Stolz L E, Hübner C, Riebel T, Kömen W, Lee S J. 2004. Myostatin mutation associated with gross muscle hypertrophy in a child. New England Journal of Medicine, 350(26), 2682–2688, http:// doi:10.1056/nejm200409023511018
Sumantri C R, Diyono A, Farajallah, I Inounu. 2008. Polymorphism of calpastatin gene and its effect on body weight of local sheep’s. JITV. 13:117-126.
Zhou H, Hickford J G H, Fang Q. 2008. Variation in the coding region of the Myostatin (GDF8) gene in sheep. Molecular and Cellular Probes, 22, 67- 68, http:// doi: 10.1016/j.mcp.2007.08.004
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