Characterization of Bacillus spp. from the rhizosphere of potato Granola varieties as an antibacterial against Ralstonia solanacearum
##plugins.themes.bootstrap3.article.sidebar##
Issue
Section
Articles
##plugins.themes.bootstrap3.article.main##
Abstract
Abstract. Prihatiningsih N, Arwiyanto T, Hadisutrisno B, Widada J. 2020. Characterization of Bacillus spp. from the rhizosphere of potato Granola varieties as an antibacterial against Ralstonia solanacearum. Biodiversitas 21: 4199-4204. Ralstonia solanacearum is one of the important wilt pathogens that significantly decreased potato yields in Indonesia. One of the strategies to control wilt disease is the use of Bacillus sp. that isolated from potato rhizosphere. Bacillus sp. is an antagonist against some phytopathogenic fungi and bacteria. The objectives of the study were to identify 5 strains of Bacillus spp. (B46, B209, B211, B298, and B315) that is capable of suppressing the growth of R. solanacearum. Experimental assays were performed to determine the growth inhibition of R. solanacearum by culturing in a two-layer medium. The identification of Bacillus spp. was performed based on physiological and biochemical characters and molecular identification. Results showed that all of Bacillus spp. are capable of suppressing the growth of R. solanacearum. B. subtilis B315 isolate was the most effective biocontrol agent in inhibiting R. solanacearum growth by a bacteriostatic mechanism. All Bacillus spp. have similarities in physiological and biochemical characters. Based on the molecular analysis, Bacillus sp. B46, B209, B211, and B298 were homologous to B. subtilis subspecies spizizeni RRLKE2, whereas Bacillus sp. B315 was homologous to B. subtilis strain WIFD5.
##plugins.themes.bootstrap3.article.details##
References
Abusham RA, Rahman RNZRA , Salleh AB, Basri M. 2009. Optimization of physical factors affecting the production of thermo-stable organic solvent-tolerant protease from a newly isolated halo tolerant Bacillus subtilis strain Rand. Microbial Cell Factories 8(20): 1-9.
Amin A, Khan MA, Ehsanullah M, Haroon U, Azam SMF, Hameed A. 2012. Production of peptide antibiotics by Bacillus sp. GU 057 indigenously isolated from saline soil. Brazilian Journal of Microbiology : 1340-1346.
Ashiru AW, Teniola OD, Dibiana NN, Apena A. 2012. Isolation of starch degrading spoilage bacteria from “Ogi” (Fermenting Maize Starch). Pakistan Journal of Nutrition 11(3): 243-246.
Calvo P, Ormeño-Orrillo E, Martínez-Romero E, ZúñigaI D.2010. Characterization of Bacillus isolates of potato rhizosphere from andean soils of Peru and their potential PGPR characteristics. Braz J Microbiol, 41: 899-906
Chun W, Vidaver AK. 2001. Gram Positive Bacteria: Bacillus. Pp: 250-260. In: Schaad NW, Jones JB, and Chun W (Eds.). Plant Pathogenic Bacteria 3nd. ed. APS Press. St.Paul Minnesota.
Fahy PC, Hayward AC. 1983. Media and Methods for Isolation and Diagnostic Test. Pp: 337-378. In: P.C. Fahy & G.J. Persley (Eds.) Plant Bacterial Diseases A Diagnostic Guide. Academic Press. London.
Ghosh S, Sinha A, Sahu C. 2007 Isolation of putative probionts from the intestines of Indian mayor carps. The Israel Journal of Aquaculture-Bamigdeh. 59(3): 127-132
Goto M (1992) Fundamentals of Bacterial Plant Pathology. Academic Press, INC. New York. 339 pp.
Irfan M, Nadeem M,. Syed QA, Baig S. 2011. Production of Thermostable ?-amylase from Bacillus sp. in Solid State Fermentation. J. Appl. Sci. Res.7(5): 607-617.
Jamil B, Hasan F, Hameed A, Ahmed S. 2007. Isolation of Bacillus subtilis MH-4 from Soil and its potential of Polypeptidic Antibiotic Production. Pak.Journal.Pharm.Sci. 20(1): 26-31
Khan JA, Priya R. 2011).A Study on Partial Purification and Characterization of Extracellular Amylases from Bacillus subtilis. Adv. Appl. Sci.Res. 2(3): 509-519
Kohl J, Kolnaar R and Ravensberg WJ.2019. Mode of Action of Microbial Biological Control agents against Plant Diseases: Relevance Beyond Efficacy. Frontiers in Plant Science. 10: 1-19
Lelliot RA, Stead DE. 1987. Methods for The Diagnosis of Bacterial Diseases of Plant. British Society for Plant Pathology by Blackwel Scientific Publication, Melbourne. 216 pp.
Lenteren JC, Bolckmans K. Ko¨hl J. 2018. Biological control using invertebrates and microorganisms:
plenty of new opportunities. BioControl (2018) 63:39–59. https://doi.org/10.1007/s10526-017-9801-4
Mugiastuti E, Suprayogi, Prihatiningsih N, Soesanto L. 2020. Isolation and characterization of the endophytic bacteria, and their potential as maize diseases control. BIODIVERSITAS 21 (5): 1809-1815, May 2020
Muthoni J, Shimelis H, Melis R. 2012. Management of Bacterial Wilt [Rhalstonia solanacearum Yabuuchi et al., 1995] of Potatoes: Opportunity for Host Resistance in Kenya. Journal of Agricultural Science; 4(9): 64-78
Pandey A, Nigam P, Soccol CR, Soccol VT, Singh D, Mohan R. 2000. Advances in Microbial Amylases. Biotechnol. Appl. Biochem. 31(2): 135-152.
Prihatiningsih N, Soedarmono, Arwiyanto T, Hadisutrisno B. 2006. Pengendalian Hayati Penyakit Layu Bakteri Kentang dengan Bacillus sp.: 1. Eksplorasi dan pengujian in vitro dan di rumah plastik. Agrosains 8(1): 27-31
Prihatiningsih N, Djatmiko HA, Rochminarsi E. 2011. Application of Potassium Fertilizer and Bacillus sp. Biopesticide for Increasing Tomato Resistance to Bacterial Wilt Disease. Agrivita. 33 (1): 8-14
Prihatiningsih N, Djatmiko HA. 2016. Enzim Amilase sebagai komponen antagonis Bacillus subtilis B315 terhadap Ralstonia solanacearum kentang. Jurnal Hama dan Penyakit Tumbuhan Tropika 16 (1) : 10-16
Prihatningsih N, Djatmiko HA, Erminawati. 2019. Bio-management of anthracnose disease in chilli with microencapsulates containing Bacillus subtilis B298 IOP Conference Series: Earth and Environmental Science 250 (2019) 012041
Prihatiningsih N, Erminawati, Djatmiko HA. 2020. Shelf-life of Bacillus subtilis B298 inclusion in biopesticide microencapsule formula and its efficacy in suppressing anthracnose disease on chili. IOP Conf. Series: Earth and Environmental Science 468 (2020) 012027
Sands DC. 1990. Physiological Criteria-Determinative Test. Pp: 133-143. In: Z. Klement, K. Rudolph & D.C. Sands (Eds.) Methods in Phytobacteriology. Academiai Kiado, Budapest.
Schaad NW. 2001. Initial Identification of Common Genera. Pp: 1-13 In: N.W. Schaad, J.B. Jones & W. Chun (Eds.), Laboratory Guide for Identification of Plant Pathogenic Bacteria. APS Press, St. Paul. Minnesota.
Sharma S, Kaur M. 2010 Antimicrobial activities of rhizobacterial strains of Pseudomonas and Bacillus strain isolated from rhizosphere soil of carnation (Dianthus caryophyllus cv. Sunrise). Indian J. Microbiol 50: 229-232.
Singh D, Yadav DK, Chaudhary G, Rana VS, Sharma RK. 2016. Potential of Bacillus amyloliquefaciens for Biocontrol of Bacterial Wilt of
Tomato Incited by Ralstonia solanacearum. J Plant Pathol Microbiol 7: 327. doi:10.4172/2157-7471.1000327
Slepecky RA, Hemphill HE. 1992. The Genus Bacillus-Nonmedical. Pp: 1663-1696. In, A. Balows, H.G. Truper, M. Dworkin, W. Harder and K-H Schleifer (Eds.), The Prokaryotes 2nd ed. A Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Aplications. Springer-Verlag. New York Inc.
Slepecky RA, Hemphill HE. 2006. The Genus Bacillus-Nonmedical. Pp: 530-562. In, A. Balows, H.G. Truper, M. Dworkin, W. Harder and K-H Schleifer (Eds.), The Prokaryotes 4th ed. A Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Aplications. Springer-Verlag. New York Inc.
Yabuuchi, E., Kosako, Y., Yano, L., Hotta, H., and Nishiuchi, Y. 1995. Transfer of two Bulkholderia and an Alcaligenes species to Ralstonia gen. nov.:
Proposal of Ralstonia pickettii (Ralston, Palleroni, and Doudoroff 1973) comb. nov., Ralstonia solanacearum (Smith 1896) comb. nov. Microbiol.
Immunol. 39:897–904.
Yanti Y, .Hamid H, Reflin, Warnita and Habazar T. 2020. The ability of indegenous Bacillus spp. consortia to control the anthracnose disease
(Colletotrichum capsici) and increase the growth of chii plants. Biodiversitas 21(1): 197-186
Amin A, Khan MA, Ehsanullah M, Haroon U, Azam SMF, Hameed A. 2012. Production of peptide antibiotics by Bacillus sp. GU 057 indigenously isolated from saline soil. Brazilian Journal of Microbiology : 1340-1346.
Ashiru AW, Teniola OD, Dibiana NN, Apena A. 2012. Isolation of starch degrading spoilage bacteria from “Ogi” (Fermenting Maize Starch). Pakistan Journal of Nutrition 11(3): 243-246.
Calvo P, Ormeño-Orrillo E, Martínez-Romero E, ZúñigaI D.2010. Characterization of Bacillus isolates of potato rhizosphere from andean soils of Peru and their potential PGPR characteristics. Braz J Microbiol, 41: 899-906
Chun W, Vidaver AK. 2001. Gram Positive Bacteria: Bacillus. Pp: 250-260. In: Schaad NW, Jones JB, and Chun W (Eds.). Plant Pathogenic Bacteria 3nd. ed. APS Press. St.Paul Minnesota.
Fahy PC, Hayward AC. 1983. Media and Methods for Isolation and Diagnostic Test. Pp: 337-378. In: P.C. Fahy & G.J. Persley (Eds.) Plant Bacterial Diseases A Diagnostic Guide. Academic Press. London.
Ghosh S, Sinha A, Sahu C. 2007 Isolation of putative probionts from the intestines of Indian mayor carps. The Israel Journal of Aquaculture-Bamigdeh. 59(3): 127-132
Goto M (1992) Fundamentals of Bacterial Plant Pathology. Academic Press, INC. New York. 339 pp.
Irfan M, Nadeem M,. Syed QA, Baig S. 2011. Production of Thermostable ?-amylase from Bacillus sp. in Solid State Fermentation. J. Appl. Sci. Res.7(5): 607-617.
Jamil B, Hasan F, Hameed A, Ahmed S. 2007. Isolation of Bacillus subtilis MH-4 from Soil and its potential of Polypeptidic Antibiotic Production. Pak.Journal.Pharm.Sci. 20(1): 26-31
Khan JA, Priya R. 2011).A Study on Partial Purification and Characterization of Extracellular Amylases from Bacillus subtilis. Adv. Appl. Sci.Res. 2(3): 509-519
Kohl J, Kolnaar R and Ravensberg WJ.2019. Mode of Action of Microbial Biological Control agents against Plant Diseases: Relevance Beyond Efficacy. Frontiers in Plant Science. 10: 1-19
Lelliot RA, Stead DE. 1987. Methods for The Diagnosis of Bacterial Diseases of Plant. British Society for Plant Pathology by Blackwel Scientific Publication, Melbourne. 216 pp.
Lenteren JC, Bolckmans K. Ko¨hl J. 2018. Biological control using invertebrates and microorganisms:
plenty of new opportunities. BioControl (2018) 63:39–59. https://doi.org/10.1007/s10526-017-9801-4
Mugiastuti E, Suprayogi, Prihatiningsih N, Soesanto L. 2020. Isolation and characterization of the endophytic bacteria, and their potential as maize diseases control. BIODIVERSITAS 21 (5): 1809-1815, May 2020
Muthoni J, Shimelis H, Melis R. 2012. Management of Bacterial Wilt [Rhalstonia solanacearum Yabuuchi et al., 1995] of Potatoes: Opportunity for Host Resistance in Kenya. Journal of Agricultural Science; 4(9): 64-78
Pandey A, Nigam P, Soccol CR, Soccol VT, Singh D, Mohan R. 2000. Advances in Microbial Amylases. Biotechnol. Appl. Biochem. 31(2): 135-152.
Prihatiningsih N, Soedarmono, Arwiyanto T, Hadisutrisno B. 2006. Pengendalian Hayati Penyakit Layu Bakteri Kentang dengan Bacillus sp.: 1. Eksplorasi dan pengujian in vitro dan di rumah plastik. Agrosains 8(1): 27-31
Prihatiningsih N, Djatmiko HA, Rochminarsi E. 2011. Application of Potassium Fertilizer and Bacillus sp. Biopesticide for Increasing Tomato Resistance to Bacterial Wilt Disease. Agrivita. 33 (1): 8-14
Prihatiningsih N, Djatmiko HA. 2016. Enzim Amilase sebagai komponen antagonis Bacillus subtilis B315 terhadap Ralstonia solanacearum kentang. Jurnal Hama dan Penyakit Tumbuhan Tropika 16 (1) : 10-16
Prihatningsih N, Djatmiko HA, Erminawati. 2019. Bio-management of anthracnose disease in chilli with microencapsulates containing Bacillus subtilis B298 IOP Conference Series: Earth and Environmental Science 250 (2019) 012041
Prihatiningsih N, Erminawati, Djatmiko HA. 2020. Shelf-life of Bacillus subtilis B298 inclusion in biopesticide microencapsule formula and its efficacy in suppressing anthracnose disease on chili. IOP Conf. Series: Earth and Environmental Science 468 (2020) 012027
Sands DC. 1990. Physiological Criteria-Determinative Test. Pp: 133-143. In: Z. Klement, K. Rudolph & D.C. Sands (Eds.) Methods in Phytobacteriology. Academiai Kiado, Budapest.
Schaad NW. 2001. Initial Identification of Common Genera. Pp: 1-13 In: N.W. Schaad, J.B. Jones & W. Chun (Eds.), Laboratory Guide for Identification of Plant Pathogenic Bacteria. APS Press, St. Paul. Minnesota.
Sharma S, Kaur M. 2010 Antimicrobial activities of rhizobacterial strains of Pseudomonas and Bacillus strain isolated from rhizosphere soil of carnation (Dianthus caryophyllus cv. Sunrise). Indian J. Microbiol 50: 229-232.
Singh D, Yadav DK, Chaudhary G, Rana VS, Sharma RK. 2016. Potential of Bacillus amyloliquefaciens for Biocontrol of Bacterial Wilt of
Tomato Incited by Ralstonia solanacearum. J Plant Pathol Microbiol 7: 327. doi:10.4172/2157-7471.1000327
Slepecky RA, Hemphill HE. 1992. The Genus Bacillus-Nonmedical. Pp: 1663-1696. In, A. Balows, H.G. Truper, M. Dworkin, W. Harder and K-H Schleifer (Eds.), The Prokaryotes 2nd ed. A Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Aplications. Springer-Verlag. New York Inc.
Slepecky RA, Hemphill HE. 2006. The Genus Bacillus-Nonmedical. Pp: 530-562. In, A. Balows, H.G. Truper, M. Dworkin, W. Harder and K-H Schleifer (Eds.), The Prokaryotes 4th ed. A Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Aplications. Springer-Verlag. New York Inc.
Yabuuchi, E., Kosako, Y., Yano, L., Hotta, H., and Nishiuchi, Y. 1995. Transfer of two Bulkholderia and an Alcaligenes species to Ralstonia gen. nov.:
Proposal of Ralstonia pickettii (Ralston, Palleroni, and Doudoroff 1973) comb. nov., Ralstonia solanacearum (Smith 1896) comb. nov. Microbiol.
Immunol. 39:897–904.
Yanti Y, .Hamid H, Reflin, Warnita and Habazar T. 2020. The ability of indegenous Bacillus spp. consortia to control the anthracnose disease
(Colletotrichum capsici) and increase the growth of chii plants. Biodiversitas 21(1): 197-186
Most read articles by the same author(s)
- HERU ADI DJATMIKO, DHADHANG WAHYU KURNIAWAN, NUR PRIHATININGSIH, Potential of Bacillus subtilis potato isolate as biocontrol agent of Xanthomonas oryzae pv. oryzae and candidate for nanosuspension formula , Biodiversitas Journal of Biological Diversity: Vol. 23 No. 7 (2022)
- NUR PRIHATININGSIH, ARI ASNANI, HERU ADI DJATMIKO, Extracellular protease from Bacillus subtilis B315 with antagonistic activity against bacterial wilt pathogen (Ralstonia solanacearum) of chili , Biodiversitas Journal of Biological Diversity: Vol. 22 No. 3 (2021)
- EMA DAMAYANTI, PUSPITA LISDIYANTI, ANDINI SUNDOWO, SHANTI RATNAKOMALA, ACHMAD DINOTO, JAKA WIDADA, MUSTOFA, Antiplasmodial activity, biosynthetic gene clusters diversity, and secondary metabolite constituent of selected Indonesian Streptomyces , Biodiversitas Journal of Biological Diversity: Vol. 22 No. 6 (2021)
- LOEKAS SOESANTO, BISIRIL FATIHAH, ABDUL MANAN, ENDANG MUGIASTUTI, NUR PRIHATININGSIH , Organic control of Bemisia tabaci Genn. on Capsicum annuum with entomopathogenic fungi raw secondary metabolites , Biodiversitas Journal of Biological Diversity: Vol. 21 No. 12 (2020)
- LISA NAVITASARI, TRI JOKO, RUDI HARI MURTI, TRIWIDODO ARWIYANTO, Rhizobacterial community structure in grafted tomato plants infected by Ralstonia solanacearum , Biodiversitas Journal of Biological Diversity: Vol. 21 No. 10 (2020)
- HADIWIYONO HADIWIYONO, JAKA WIDADA, SITI SUBANDIYAH, MARK FEGAN, Pulsed Field Gel Electrophoresis (PFGE): a DNA finger printing technique to study the genetic diversity of blood disease bacterium of banana , Biodiversitas Journal of Biological Diversity: Vol. 12 No. 1 (2011)
- NUR PRIHATININGSIH, HERU ADI DJATMIKO, PUJI LESTARI, Endophytic bacteria associated with rice roots from suboptimal land as plant growth promoters , Biodiversitas Journal of Biological Diversity: Vol. 22 No. 1 (2021)
- TRIWIDODO ARWIYANTO, YMS MARYUDANI, NINING NURUL AZIZAH, Phenotypic charactheristics of fluorescent pseudomonss, biological control agent of lincat disease of temanggung tobacco , Biodiversitas Journal of Biological Diversity: Vol. 8 No. 2 (2007)
- NUR PRIHATININGSIH, RUTH FETI RAHAYUNIATI, HERU ADI DJATMIKO, PUJI LESTARI, NUR KHOLIDA WULANSARI, I KETUT WIDNYANA, KOKO DWI SUTANTO, Endophytic bacterial isolate diversity in suboptimal field rice and their potential in sheath blight control , Biodiversitas Journal of Biological Diversity: Vol. 25 No. 8 (2024)
- ANGGI ANWAR HENDRA NURDIKA, TRIWIDODO ARWIYANTO, SRI SULANDARI, Physio-biochemical, molecular characterization, and phage susceptibility of Ralstonia pseudosolanacearum associated with tomato (Solanum lycopersicum) and eggplant (Solanum melongena) , Biodiversitas Journal of Biological Diversity: Vol. 23 No. 10 (2022)