Azolla microphylla and Pseudomonas aeruginosa for bioremediation of bioethanol wastewater




Abstract. Annisa K, Sutarno, Santosa S. 2021. Azolla microphylla and Pseudomonas aeruginosa for bioremediation of bioethanol wastewater. Biodiversitas 22: 1799-1805. Bioremediation is the right choice in wastewater treatment since it requires has low cost but works optimally. Azolla microphylla and Pseudomonas aeruginosa are considered to have the ability to optimize waste degradation. The aim of this study was to determine the best treatment for reducing pollutants in bioethanol waste, namely by using A. microphylla and P. aeruginosa. The twelve treatments in this experimental research included variations in the A. microphylla biomass and variations of biomass combinations with the density of the P. aeruginosa. Research method used was completely randomized design with three replications, and all 36 samples were taken on the seventh day. Research data collected were the quality parameters of bioethanol wastewater before and after the treatment. The results showed that TSS, BOD, COD were decreased during seven days of treatment, whereas pH and H2S levels were increased. The combination of 0.2 kg A. microphylla and 1010 cfu/mL P. aeruginosa showed the best result in reducing pollutants. This study showed that A. microphylla and P. aeruginosa could be used to reduce pollutants in bioethanol wastewater. Results of the research are expected to become an alternative solution to the bioethanol wastewater problem.


Alkorta I, Hernández-Allica J, Becerril JM, Amezaga I, Albizu I, Garbisu C. 2004. Recent findings on the phytoremediation of soils contaminated with environmentally toxic heavy metals and metalloids such as zinc, cadmium, lead, and arsenic. Reviews in Environ Sci Biotechnol 3 (1): 71-90.
Alwathan, Mustafa, Thahir R. 2013. Reduction of H2S levels of wastewater biogas hospital with adsorption method. Conversion 2: 1-6 [Indonesian].
Arimby C, Lestari W, Azis Y. 2014. The utilization of Azolla pinnata R.Br in Zn absorption from wastewater rubber factory as fitoremediator. JOM MIPA 1: 1-8 [Indonesian].
Arora A, Saxena S and Sharma DK. 2006. Tolerance and phytoaccumulation of Chromium by three Azolla species. World journal of microbiology & biotechnology 22: 97–100
Asih DW, Rachmadiarti F. 2019. Azolla microphylla as metal fitoremediator Pb. LenteraBio 8: 85-90. [Indonesian]
Astriani M, Zubaidah S, Abadi AL, Suarsini E. 2020. Pseudomonas plecoglossicida as a novel bacterium for phosphate solubilizing and indole-3-acetic acid-producing from soybean rhizospheric soils of East Java, Indonesia. Biodiversitas 21: 578-586.
Astuti W, Mahatmanti Widhi. 2017. Manufacture of liquid fermentation fertilizer vinasse based. Engineering 15: 55-58. [Indonesian]
Austin, B. 1988. Methods in Aquatic Bacteriology. John Willey&Sons.Thomsosn Press (India) Ltd., New Delhi.
Bhore SJ, Sathisha G. 2010. Screening of endophytic colonizing bacteria for cytokinin-like compounds: crude cell-free broth of endophytic colonizing bacteria is unsuitable in cucumber cotyledon bioassay. J Agricult Sci 6: 345-352.
Carrapiço F. 2017. The azolla–anabaena–bacteria association: a case of symbiotic abduction? In: Muggia L, Seckbach J, Grube M (eds) Algal and cyanobacteria symbioses. World scientific publishing company. Portugal
Castignetti D, Hollocher TC. 1984. Heterotrophic nitrification among denitrifiers. Applied and environmental microbiology 47: 620-623
Chandekar NS, Godboley BJ. 2015. Use of Phytoremediation for the Treatment of Kitchen Wastewater. International Journal of Science and Research (IJSR) 6: 1170-1173
Dhamayanthie I. 2000. Wastewater processing industrial textile with anaerob process. [Thesis] ITB, Bandung [Indonesian].
Effendi, H. 2003. Studying water quality, for resource management and aquatic environment. Kanisius. Yogyakarta. [Indonesian].
Ekta P, Modi NR. 2018. A review of phytoremediation. Journal of Pharmacognosy and Phytochemistry 7: 1485-1489
Ekyastuti W, Ekamawanti HA. 2018. Short Communication: The role of microbial rhizosphere in enhancing plant growth of Jatropha curcas in soil contaminated mercury. Biodiversitas 19: 701-705.
Etim EE. 2012. Phytoremediation and its mechanisms; a review. Int J Environ Bioenergy 2: 120-136.
Furini A, Manara A and DalCorso G. 2015. Editorial: Environmental phytoremediation: plants and microorganisms at work. Frontiers in Plant Science 6:520
Germaine KJ, Byrne J, Liu X, Keohane J, Culhane J, Lally RD. 2015. Ecopiling: a combine phytoremediation and passive biopiling system for remediating hydrocarbon impacted soils at field scale. Frontiers in Plant Science 5:756
Ghosh M, Singh SP. 2005. A Review on Phytoremediation of Heavy Metals and Utilization of Its by Products. Asian Journal on Energy and Environment 6: 214-231
Hayati N. 1992. Ability of water hyacinth in changing the physical properties of wastewater chemical fertilizer plant urea and acid formic. [Thesis] ITB, Bandung. [Indonesian].
Hidayati N, Rini DS. 2020. Assessment of plants as lead and cadmium accumulators for phytoremediation of contaminated rice field. Biodiversitas 21: 1928-1934.
Jawetz M, Adelberg’s G, Brooks F, Janet S, Butel, Stefhen. 2010. Medical microbiology. McGraw-hill companies inc, United States of Amerika.
Jenie BSL dan Rahayu WP. 1996. Waste handling of food industry.
Kanisius, Yogyakarta. [Indonesian]
Khan MU, Sessitsch A, Harris M, Fatima K, Imran A, Arslan M. 2015. Cr resistant rhizo and endophytic bacteria associated with Prosopis juliflora and their potential as phytoremediation enhancing agents in metaldegraded soils. Frontiers in Plant Science 5:755
Kurniawati Y, Wardoyo SE, Arizal R. 2015. Optimization of the use of electrolyte salts as liquid clear. Natural science journals Nusa Bangsa University 5: 30-4. [Indonesian].
Lestari, SU, Mutryarny E, Susi N. 2019. Azolla mycrophylla fertilizer for sustainable agriculture: compost and liquid fertilizer applications. International journal of scientific & technology research 8: 542-547.
Lestari, SU. 2018. Analysis of some elements of chemical compost Azolla mycrophylla. Agricultural scientific journals 14: 60-5. [Indonesian].
Limmer M dan Burken J. 2016. Phytovolatilization of Organic Contaminants. Environmental Science and Technology 50: 6632?6643
Mardhiana, Pradana AP, Adiwena M, Santoso D, Wijaya R, Murtilaksono A. 2017. Use of endophytic bacteria from roots of Cyperus rotundus for biocontrol of Meloidogyne incognita. Biodiversitas 18: 1308-1315.
Markl H. 1999. Modeling of biogas reactors. In: WINTER, J.ed.Biotechnology. 2nd New York: A Wiley Company 11: 527- 560.
Maslahat M , Rakhmanika F , Sutamihardja RTM. 2010. Potency of Pseudomonas aeruginosa bacteria in the process of biodegradation of benzene linear sulfonate (LAS) in three types of commercial deterties. Ecolab 4: 55-96. [Indonesian].
Maulana MF, Haniswita. 2016. Implementation of Biofertilizer Azolla pinnata in Indonesian rice production system: Efforts to achieve food security for sustainable development. [Indonesian].
Mentari A, Probosunu N & Adharini RI. 2016. Utilization of Azolla sp. to lower the COD (Chemical Oxygen Demand) content in laundry waste. Fisheries Journal UGM 18: 67-72 [Indonesian].
Morrice, J.A., Danz, N.P., Regal, R.R., Kelly, J.R., Niemi, G.J., Reavie, E.D., Hollenhorst, T.P., Axler, R.P., Trebitz, A.S., Cotter, A.M. and Peterson, G.S. 2008. Human influences on water quality in Great Lakes coastal wetlands. Environmental Management 41:347-357
Napaldet JT, Buot Jr. IE, Zafaralla MT, Lit Jr. IL, Sotto RC. 2019. Effect of phytoremediation on the morpho-anatomical characters of some aquatic macrophytes. Biodiversitas 20: 1289-1302.
Ng, YS, Chan DJC. 2017.Wastewater phytoremediation by Salvinia molesta. Journal of Water Process Engineering 15: 107-115.
Noorjahan CM, Jamuna S. 2015. Biodegradation of sewage waste water using Azolla microphylla and its reuse for aquaculture of fish tilapia Mossambica. IOSR Journal of environmental science, toxicology and food technology 9: 75-80.
Nuraini Y dan Felani M. 2015. Phytoremediation of tapioca wastewater using water hyacinth plant (Eichhornia crassipes). Journal of degraded and mining lands management 2: 295-302
Nurcahyani, K. 2015. Treatment of wastewater of the alcohol industry using fermentation process. Proceeding of national seminary conservation and utilization of natural resources. Sebelas Maret University, Surakarta, 13 January 2015. [Indonesian].
Padmaningrum RT, Aminatun T, Yuliati. 2014. The effect of the biomass of jasmine water (Echinodorus paleafolius) and Lotus (Nyphaea firecrest) to phosphate levels, BOD, COD, TSS, and the acidity of the wastewaterwater laundry. Saintek Research Journals 19: 64-74 [Indonesian].
Parbo AP, Irwan E, Syahril N. 2019. The effect of giving detergents to the growth of Azolla microphylla in brackish water. Asian journal of aquatic sciences 2: 145-152.
Prabu PC, Udayasoorian C. 2007. Treatment of Pulp and Paper Mill Effluent Using Constructed Wetland. Electronic Journal of Enviromental, Agricultural and Food Chemistry 6: 1689-1701
Qaisar M, Ping Z, Jing C, Yousaf H, Jaffar HM, Dong-lei WU, Bao-lan HU. 2007. Journal of Zhejiang University SCIENCE A 8:1126- 1140
Qin H, Zhang Z, Liu M, Liu H, Wang Y, Wen X, Yan S. 2016. Site test of phytoremediation of an open pond contaminated with domestic sewage using water hyacinth and water lettuce. – Ecological Engineering 95: 753-762.
Qu, M., Li, H., Li, N., Liu, G., Zhao, J., Hua, Y., Zhu, D. (2017):
Distribution of atrazine and its phytoremediation by submerged macrophytes in lake sediments. – Chemosphere 168: 1515-1522.
Rahardja BS, Prayogo, Mahasri G dan Hardhianto MD. The effectiveness of Pseudomonas bacteria as the parser of organic matter (proteins, carbohydrates, fats) on the water waste sewage Dumbo catfish Hatchery (Clarias sp.) closed recirculation system. Fisheries and marine scientific journals 2: 159-164. [Indonesian].
Rahmadian CA, Ismail , Abrar M, Erina, Rastina, Fahrimal Y. 2018. Isolation and identification of bacteria Pseudomonas sp. in salted fish in the place of fish auction in Labuhanhaji Selatan Aceh. JIMVET 2: 493-502. [Indonesian].
Rao, J.F. 1994. Enzymes in the hydrolysis and ol/otbemig of starch. In: Starch: Chemistry and technology. 2" ed. Eds. Whistler, R.L., J.N. Bemiller & E.F. Paschall. Academic Press. Inc., Orlando.
Retnaningdyah C, Arisoesilaningsih E, Samino S. 2017. Use of local Hydromacrophytes as phytoremediation agent in pond to improve irrigation water quality evaluated by Diatom Biotic Indices. Biodiversitas 18: 1596-1602
Riaz, G., Tabinda, A. B., Iqbal, S., Yasar, A., Abbas, M., Khan, A. M., Baqar, M. (2017): Phytoremediation of organochlorine and pyrethroid pesticides by aquatic macrophytes and algae in freshwater systems. International Journal of Phytoremediation 19(10): 894- 898.
Rohmah, SA, Hari RIW, Nur H. 2019. Azolla pinnata plant Efficiency in lowering COD (Chemical Oxygen Demand) in liquid vermicelli waste at Arcawinangun Village East Purwokerto District of Banyumas in 2018. Keslingmas 38: 1-123. [Indonesian].
Sanchez G, Alcantara S, Razoflores E, Revah S. 2005. Oxygen transfer and consumption in a thiosulfate oxidizing bioreactor with sulfur production. Letters in Applied Microbiology 41: 141-146.
Sanna A, Steel L, Valer MMM. 2017. Carbon dioxide sequestration using NaHSO4 and NaOH: A dissolution and carbonation optimisation study. Journal of Environmental Management 189: 84- 97
Santosa S, Sutarno, Purwanto E, Suranto, Sajidan. 2018. Molecular characterization of Plant Growth Promoting Rhizobacteria using 16S rRNA sequences in the organic rice field of Sukorejo Village, Central Java, Indonesia. Biodiversitas 19: 2157- 2162.
Setiawati MR, Herdiyantoro D, Damayani M, dan Suryatmana P. 2018. Analysis of C, N, C/N ratio of soil and yield of fertilizer rice organic and Azolla-based biofertilizer on organic rice fields. Soilrens 16: 30-36. [Indonesian].
Shah, MP, Patel KA, Darji AM. 2013. Microbial degradation and decolorization of methyl orange dye by an aplication of Pseudomonas spp. ETL-1982. J. of Environmental Bioremediation & Biodegradation. 1: 26-36.
Sharma A, Sachdeva S. 2015. Assessing the phytoremediation potential and the metabolic responses of Azolla microphylla on lead exposure. International journal of scientific research 4: 255-258.
Sheena KN, Harsha P. 2018. Feasibility Study of Phytoremediation in Wastewater Treatment. International Journal of Science and Research (IJSR) 7: 1019-1026
Soman D, Anitha V, Arora A. 2018. Bioremediation of municipal sewage water with Azolla microphylla. International Journal of Advanced Research 6: 101-108.
Sood APL, Uniyal R, Rasana ASA, 2011. Phytoremediation Potential of Aquatic Macrophyte, Azolla. Ambio 41: 122-137
Suhendrayatna, Marwan, Andriani R, Fajriana Y, and Elvitriana. 2012. Removal of Municipal Wastewater BOD, COD, and TSS by Phyto- Reduction: A Laboratory–Scale Comparison of Aquatic Plants at Different Species Typha Latifolia and Saccharum Spontaneum. International Journal of Engineering and Innovative Technology (IJEIT) 2: 333-337
Sulistiyaningsih. 2010. Sensitivity test of multiple antiseptic preparations against multi-resistant Pseudomonas aeruginose bacteria (PAMR). [Thesis]. Universitas Padjadjaran, Jatinangor. [Indonesian].
Supartoto, Widyasunu1 P, Rusdiyanto, Santoso M. 2012. Exploration of the potential of Azolla Microphylla and Lemna Polyrhizza as a manufacturer of biomass green fertilizer, feed ducks and fish. The proceedings of the National Seminar on rural resource development and sustainable local wisdom II, Purwokerto, 27-28 November 2012. [Indonesian].
Surdina E, El-Rahimi S, Hasri I. 2016. Azolla microphylla growth with a combination of cattle manure. Journal of Marine Students and Unsyiah fisheries scientific Journals 1: 298-306. [Indonesian].
Sutanto A. 2011. Degradation of the organic material of pineapple wastewater by indigenous bacteria. El-Hayah 1: 151-156 [Indonesian].
Syahputra K, Rusmana I, dan Widyastuti U. 2011. Isolation and characterization of denitrification bacteria as inorganic nitrogen bioremediation agents. J. Ris. Akuakultur 6: 197-209 [Indonesian].
Syamsiyah J, Sunarminto BH, Mujiyo. 2016. Potential of Azolla as a substitute for manure in organic rice cultivation. Caraka Tani
– Journal of Sustainable Agriculture, 31: 102-107. [Indonesian]. Tangahu BV, Ningsih DA, Kurniawan SB, Imron MF. 2019. Study of
BOD and COD Removal in Batik Wastewater using Scirpus grossus and Iris pseudacorus with Intermittent Exposure System. Journal of Ecological Engineering 20: 130-134
Tanner C. 2001. Plants as ecosystem engineers in subsurface-flow treatment wetlands. Water Science and Technology 44: 11–12
Tulod AM, Castillo AS, Carandang WM, Pampolina NM. Growth performance and phytoremediation of Pongamia pinnata (L.) Pierre, Samanea saman (Jacq.) Merr. and Vitex parviflora Juss. In copper contaminated soil amended with zeolite and VAM. Asia Life Sci 21(2): 499-522.
Ugya AY, HUA X, MA J. 2019. Phytoremediation as a tool for the remediation of wastewater resulting from dyeing activities. Applied ecology and environmental research 17: 3723-3735
Utama P, Firnia D, Natanael G. 2015. The growth and removals of nitrogen Azolla microphylla due to phosphoric administration and different water elevation. Agrologia 4: 41-52 [Indonesian].
Varghese AR and Jacob L. 2016. Phytoremediation of waterbodies using selected aquatic macrophytes Eicchornia crassipes (mart.) Solms and Pistia stratiotes, l. Asian journal of science and technology 7: 2774-2776.
Vymazal J dan Kropfelov L. 2009. A Removal of organics in constructed wetlands with horizontal sub-surface flow: a review of the field experience. Science of the Total Environment 407: 3911–3922
Vymazal J. 2007. Removal of nutrients in various types of constructed wetlands. Sci total environ 380: 48-65.
Wahyudi D, Aman AT, Handayani NSN, Soetarto ES. 2019. Differences among clinical isolates of Pseudomonas aeruginosa in their capability of forming biofilms and their susceptibility to antibiotics. Biodiversitas 20: 1450-1456.
Warzatullisna, Fitri L, Ismail YS. 2019. Potential of endophytic bacteria from rice root as potassium solvent. Biodiversitas 20: 1303-1308
Wiratno, Syakir M, Sucipto I, Pradana AP. 2019. Isolation and characterization of endophytic bacteria from roots of Piper nigrum and their activities against Fusarium oxysporum and Meloidogyne incognita. Biodiversitas 20: 682-687.
Xin Z , Ai JL , Fang JZ , Guo ZX , Gui LD, Yong GZ. 2008. Arsenic accumulation by the aquatic fern Azolla: Comparison of arsenate uptake, speciation and efflux by A. caroliniana and A. filiculoides. Environ.Poll 156: 1149–1155.
Zimmo OR, VanDerSteen NP, Gijzen HJ. 2005. Effect of Organic Surface Load on Process Performance of Pilot Scale Algae and Duckweed Based Waste Stabilization Ponds. J.Environ.Engg 131: 94- 587

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