Ultrastructure of Lejeunea spp. leaves surface in a lowland tropical urban forest of Universitas Indonesia Campus, Depok, Indonesia




Abstract. Putrika A, Nabella DM, Salamah A, Nisyawati, Dwiranti A. 2020. Ultrastructure of Lejeunea spp. leaves surface in a lowland tropical urban forest of Universitas Indonesia Campus, Depok, Indonesia. Biodiversitas 21: 4184-4191. Lejeunea is one of liverworts genera that have a wide distribution in the world. It has many variations of the character that have not been revealed, such as variations of the cell surface. The purpose of this research was to study the ultrastructure of Lejeunea spp. leaves surface in a lowland tropical urban forest of Universitas Indonesia (UI) Campus, Depok, Indonesia. Six species of Lejeunea spp. were studied, i.e., L. anisophylla, L. cocoes, L. exilis, L. papilionacea, L. catanduana, and L. curviloba. The research methods carried out in this study consisted of the sample observation using a light microscope, sample preparation for Scanning Electron Microscope (SEM) observation (fixation, post-fixation, dehydration, drying, mounting), and observations using SEM. The results of observation using a light microscope showed the smooth cell surface of all samples studied. Meanwhile, the differences between six species of Lejeunea in the UI campus could be differentiated under SEM. L. catanduana could be distinguished from other species from its cell wall thickness and texture, ornamentation types, and the number of ornamentations per cell. The texture of the cell wall of L. catanduana was the roughest than the other species due to abundant ornamentation such as papillae on its the cell wall surface. Furthermore, only this species has mamillae on the cell surface. The number of papillae or mamillae was 1-4 per cell. On the other hand, L. cocoes has the thinnest cell wall and slightly rough texture. Only this species has the simple papillae on the cell surface. Thus, the results of this study suggested that the cell surface variations in Lejeunea might be potential to be used as taxonomic characters in grouping species.


Choudhary OP, Priyanka. 2017. Scanning electron microscope: advantage and disadvantage in imaging component. Int J Curr Microbiol Appl Sci. 6(5): 1877-1882. doi: 10.20546/ijcmas.2017.605.207.
Dwiranti A, Masri F, Rahmayenti DA, Putrika, A. 2019. The effects of osmium tetroxide post-fixation and drying step on leafy liverwort ultrastructure study by scanning electron microscopy. Microsc Res Tech 6(3): 1-6. doi:10.1002/jemt.23251.
Gradstein SR. 2011. Guide to the liverworts and hornworts of Java. Bogor, Indonesia. Southeast Asian Regional Centre for Tropical Biology, Bogor.
Heinrichs J, Anton H, Gradstein SR, Mues R, Holz I. 2000. Surface wax: a new taxonomic feature in Plagiochilaceae. Plant Syst Evol 5 (2): 225-233.
Konrat AH, Söderström L, Mutke J, Renner M, Gradstein SR, Engel J, Zhu RL, Pickering J. 2008. Early land plants today: global patterns of liverwort diversity, distribution, and floristic knowledge. In Mohamed H, Baki BB, Nasrulhaq-Boyce A, Lee PKY, eds. Bryology in the New Millennium. Institute of Biological Sciences University of Malaya and International Association of Bryologists, Kuala Lumpur.
Kou J, Feng C, Bai X, Chen H. 2014. Morphology and taxonomy of leaf papillae and mammillae in Pottiaceae of China. J Syst Evol 52(4): 521-532. doi: 10.1111/jse.12074.
Lee GE, Gradstein SR. 2013. Distribution and habitat of the malaysian species of Lejeunea (Marchantiophyta: Lejeuneaceae), with description of Lejeunea tamaspocsii Sp. Nov. Pol Bot J 58(1): 59-69. doi: 10.2478/pbj-2013-0007.
Lee GE, Gradstein SR, Latiff A. 2011. Toward a revision of Lejeunea (Lejeuneaceae) in Malaysia. Gardens Bulletin Singapore 63 (1&2): 163-173.
Lee GE, Gradstein SR, Latiff A. 2014. New and neglected morphological features in the taxonomy of asian Lejeunea (Marchantiophyta). Pol Bot J 59(1): 31-36. doi: 10.2478/pbj-2014-0010.
Lee GE. 2020. Morphological data of Genus Lejeunea (Marchantiophyta: Lejeuneaceae) in the Malesian region. Data in Brief 28: 1-28. doi: 10.1016/j.dib.2019.104958.
Lee JT, King. 2012. SEM Sampel preparation for cells on 3D scaffolds by freeze-drying and HMDS. Scanning 34(1): 12-25. doi: 10.1002/sca.20271.
Paulo, Kellogg E. 2010. Morphology and development of leaf papillae in Sematophyllaceae. The Bryologist 113(1): 22-33. doi: 10.1639/0007-2745-113.1.22.
Putrika A, Nisyawati, Ariyanti NS. 2017. Diversity of bryophyte in the urban forest and main street of Universitas Indonesia Depok. Biosite 3(1): 25-38.
Wijaya SK. 2018. Identifikasi lumut hati epifit (leafy liverworts) di Universitas Indonesia, Depok, Jawa Barat. Skripsi. FMIPA UI, Depok.

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