Surface ultrastructure of Blastocystis sp. isolated from cattle
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Abstract. Widisuputri NKA, Lastuti NDR, Suprihati E, Hastutiek P, Plumeriastuti H, Mufasirin, Puspitasari H, Suwanti LT. 2021. Surface ultrastructure of Blastocystis sp. isolated from cattle. Biodiversitas 22: 1514-1518. Blastocystis sp is a protozoan parasite commonly detected in the intestinal tract of humans and various animals that causes zoonotic blastocystosis. The pathogenic potential of Blastocystis is still being evaluated, some Blastocystis sp are completely pathogenic, while others can be considered commensal and hypothetical, related to the role of the surface coat of Blastocystis sp. This study aimed to compare the surface ultrastructure of Blastocystis sp. in cattle with diarrhea and non diarrhea by Scanning Electron Microscope (SEM). Four Blastocystis sp. isolates were selected from the faeces of four positives cattle which consisted of two diarrhea and two non-diarrhea cattle. The result showed that Blastocystis sp. in cattle appeared in round shape and reproduced by binary fission. The surface cell of Blastocystis sp. isolates from diarrhea cattle had a rough surface while organism of non diarrhea cattle isolates was very smooth. Bacteria were seen attached to the surface of Blastocystis sp. from diarrhea cattle faeces. In conclusion, the features of the surface structure of Blastocystis sp. correlated with symptomatic appearance. The surface structure of Blastocystis sp. isolates from cattle with diarrhea was rougher than non diarrhea.
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References
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Suresh K, Howe J, Chong SY, Ng GC, Ho LC, Loh AK, Ramachandran NP, Yap EH, Singh M. 1994. Ultrastructural changes during in vitro encystment of Blastocystis hominis. Parasitol Res 80:327–335
Suwanti LT, Mufasirin, Lastuti NDR, Susana Y, Rizki SM. 2020. Molecular Detection and Morphological Varied of Blastocystis sp in Beef Cattle in Siak Sri Indrapura Riau Indonesia. Eco. Env. & Cons. 26 (April Suppl. Issue): S209- S212
Suwanti LT, Susana Y. Hastutiek, P, Suprihati E, Lastuti NDR. 2020. Blastocystis spp. subtype 10 infected beef cattle in Kamal and Socah, Bangkalan, Madura, Indonesia, Vet. World, 13(2): 231-237.
Tan TC, Suresh KG, Smith HV. 2008. Phenotypic and genotypic characterization of Blastocystis hominis isolates implicates subtype 3 as a subtype with pathogenic potential. Parasitol Res 104:85–93
Wawrzyniak I, Poirier P, Viscogliosi E, Dionigia M, Texier C, Delbac, F, Alaoui HE. 2013. Blastocystis, an unrecognized parasite: An overview of pathogenesis and diagnosis. Ther Adv Infect Dis. 1(5): 167-178. doi:10.1177/2049936113504754
Yason JA, Tan,KSW. 2018. Membrane surface features of Blastocystis Subtypes. Genes. 9: 1-6.
Zaman V, Howe J, Ng M, Goh TK. 1999. Scanning Electron Microscopy of the surface coat of Blastocystis hominis. Parasitol Res. 85: 974-976.
Zhang X, Zhang S, Qiao J, Wu X, Zhao L, Liu Y, Fan X. 2012. Ultrastructural insights into morphology and reproductive mode of Blastocystis hominis. Parasitol Res. 110:1165–1172
Boreham PFL, Stenzel DJ. 1993. Blastocystis in humans and animals: morphology, biology, and epizootiology. Adv Parasitol. 32: 1-70.
Cassidy ME, Stenzel DJ, Boreham PFL. 1994. Electron microscopy of surface structures of Blastocystis sp. from different hosts. Parasitol. 80: 505-511
de Souza W, Attias M. 2018. New Advances in Scanning Microscopy and Its Application to Study Parasitic Protozoa. Exp. Parasitol. 190: 10-33.
Mehlhorn H, Tan KSW, ? Yoshikawa H. 2012. Blastocystis: pathogen or passenger?: An evaluation of 101 years of research. Springer, Heidelberg Berlin.
Kumarasamy V, Kuppusamy UR, Jayalakshmi P, Samudi C, Ragavan ND, Kumar S. 2017 Exacerbation of colon carcinogenesis by Blastocystis sp.. PLoS ONE 12(8): e0183097. doi : 10.1371/journal.pone.0183097
Lee LL, Chye TT, Karmacharya BM, Govind SK. 2012. Blastocystis sp.: waterborne zoonotic organism, a possibility. Parasites and Vectors. 5:130
Parija SC, Jeremiah SS. 2013. Blastocystis: Taxonomy, Biology and Virulence. Tropical Parasitol. 3(1): 17-25
Ragavan ND, Govind. S.K, Chye TT, Mahadeva S. 2014. Phenotypic variation in Blastocystis sp. ST3. Parasites and Vectors. 7(404): 1-10.
Ragavan ND, Kumar S, Chye TT, Mahadeva S, Shiaw-Hooi H. 2015. Blastocystis sp. in irritable bowel syndrome (IBS)-Detection in stool aspirates during colonoscopy. PLoS ONE 10(9):e0121173. doi:10.1371/journal.pone.0121173
Suresh K, Howe J, Chong SY, Ng GC, Ho LC, Loh AK, Ramachandran NP, Yap EH, Singh M. 1994. Ultrastructural changes during in vitro encystment of Blastocystis hominis. Parasitol Res 80:327–335
Suwanti LT, Mufasirin, Lastuti NDR, Susana Y, Rizki SM. 2020. Molecular Detection and Morphological Varied of Blastocystis sp in Beef Cattle in Siak Sri Indrapura Riau Indonesia. Eco. Env. & Cons. 26 (April Suppl. Issue): S209- S212
Suwanti LT, Susana Y. Hastutiek, P, Suprihati E, Lastuti NDR. 2020. Blastocystis spp. subtype 10 infected beef cattle in Kamal and Socah, Bangkalan, Madura, Indonesia, Vet. World, 13(2): 231-237.
Tan TC, Suresh KG, Smith HV. 2008. Phenotypic and genotypic characterization of Blastocystis hominis isolates implicates subtype 3 as a subtype with pathogenic potential. Parasitol Res 104:85–93
Wawrzyniak I, Poirier P, Viscogliosi E, Dionigia M, Texier C, Delbac, F, Alaoui HE. 2013. Blastocystis, an unrecognized parasite: An overview of pathogenesis and diagnosis. Ther Adv Infect Dis. 1(5): 167-178. doi:10.1177/2049936113504754
Yason JA, Tan,KSW. 2018. Membrane surface features of Blastocystis Subtypes. Genes. 9: 1-6.
Zaman V, Howe J, Ng M, Goh TK. 1999. Scanning Electron Microscopy of the surface coat of Blastocystis hominis. Parasitol Res. 85: 974-976.
Zhang X, Zhang S, Qiao J, Wu X, Zhao L, Liu Y, Fan X. 2012. Ultrastructural insights into morphology and reproductive mode of Blastocystis hominis. Parasitol Res. 110:1165–1172
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