Characteristics of lactic acid bacteria isolated from palm sugar from West Sumatra, Indonesia and their potential as a probiotic
##plugins.themes.bootstrap3.article.sidebar##
Issue
Section
Articles
##plugins.themes.bootstrap3.article.main##
Abstract
Abstract. Ramadhanti N, Melia S, Hellyward J, Purwati E. 2021. Characteristics of lactic acid bacteria isolated from palm sugar from West Sumatra, Indonesia and their potential as a probiotic. Biodiversitas 22: 2610-2616. Palm sugar is one of the sugar products from palm sap processing. It is made by reducing the water content in the sap to certain water content and is used as a natural sweetener. This study used palm sugar from 3 producers, i.e. Marapalam Gula Semut (MGS), Tetesan Gula Semut (TGS) and Sungayang Gula Semut (SGS) in Sungayang, West Sumatra, Indonesia. This study aimed to isolate and identify lactic acid bacteria (LAB) from palm sugar, potentially as a probiotic, using the 16S rRNA method. The probiotic potential of bacteria was selected based on morphology, biochemical properties, resistance to acidic pH 3 and 0.3% bile salts, antimicrobial activity against pathogenic bacteria. The results showed that lactic acid bacteria isolated from palm sugar are Gram-positive rod-shaped (Bassil), negative catalase, and homofermentative. The total colonies of lactic acid bacteria ranged from 50 x 107 cfu/g - 117 x 107 cfu/g. The MGS lactic acid bacteria had 74.71% viability resistance in acidic pH 3, 65.52% viability against bile salts (0.3% ox gall). The diameter of inhibition against Escherichia coli O157 was 18.20 mm, Propionibacterium acnes (18.00 mm), Acinetobacter baumannii (14.19 mm), and Listeria monocytogenes (16.35 mm). The phylogenetic tree based on 16S rRNA showed that the MGS isolate has a similarity of 99.92% to Lactobacillus fermentum strain 1743. It can be concluded that Lactobacillus fermentum strain 1743 isolated from palm sugar has potential as a probiotic.
##plugins.themes.bootstrap3.article.details##
References
REFERENCES
Arena MP, Capozzi V, Spano G, Fiocco D. 2017. The potential of lactic acid bacteria to colonize biotic and abiotic surfaces and the investigation of their interactions and mechanisms. Applied Microbiology and Biotechnology 101: 2641- 2657.
Amezquita A, Brashears MM. 2002. Competitive inhibition of Listeria monocytogenes in ready-to-eat meat products by lactic acid bacteria. Journal of Food Protect 65: 316-325.
Anandharaj M, Sivasankari B. 2014. Isolation of potential probiotic lactobacillus oris HMI68 from mother’s milk with cholesterol-reducing property. Journal of Bioscience and Bioengineering 118: 153-9.
Aritonang SN, Roza E, Rossi E, Purwati E, Husmaini. 2017. Isolation and identification of lactic acid bacteria from okara and evaluation of their potential as candidate probiotics. Pakistan Journal of Nutrition 16: 618-628.
Azat R, Liu Y, Li W, Kayir A. 2016. Probiotic properties of lactic acid bacteria isolated from traditionally fermented Xinjiang cheese. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology) 17: 597-609.
Dallal SMM, Davoodabadi A, Abdi M, Hajiabdolbaghi M, Sharifi Yazdi MK, Douraghi M, Tabatabaei BSM. 2017. Inhibitory effect of Lactobacillus plantarum and Lactobacillus fermentum isolated from the faeces of healthy infants against nonfermentative bacteria causing nosocomial infections. New Microbes and New Infections 15: 9–13.
Food and Agriculture Organization of United Nations & Organization (FAO). 2002. Guidelines for the evaluation of probiotics in food. Report of a Joint FAO/WHO Working Group on Drafting Guidelines for the Evaluation of Probiotics in Food. London, London Ontario.
Harun H, Wirasti Y, Purwanto B, Purwati E. 2020. Characterization of lactic acid bacteria and determination of antimicrobial activity in dadih from Air Dingin Alahan Panjang District, Solok Regency-West Sumatera. Sys Rev Pharm 11: 583-586.
Jain N, Mehta A, Bharti V. 2017. Screening, characterization and in vitro evaluation of probiotic of Lactobacillus strains. Asian Journal of Pharmaceutical and Clinical Research 10: 288-93.
Juliyarsi I, Hartini P, Yuherman, Djamaan A, Arief, Purwanto H, Aritonang SN, Hellyward J, Purwati E. 2018. Characterization of lactic acid bacteria and determination of antimicrobial activity in tempoyak from Padang Pariaman District, West Sumatra, Indonesia. Pakistan Journal of Nutrition 17: 506-511.
Joseph GH, Payung L. 2012. Pengolahan glanular gula dari gula semut aren. Jurnal B. Palma 12: 60-65 [Indonesian]
Kocabay S, Cetinkaya S. 2020. Probiotic properties of Lactobacillus fermentum isolated from new-born faeces. Journal of Oleo Sciensce 12: 1579-1584.
Melia S, Purwati E, Yuherman, Jaswandi, Aritonang SN, Silaen M. 2017. Characterization of the antimicrobial activity of lactic acid bacteria isolated from buffalo milk in West Sumatera (Indonesia) against Listeria monocytogenes. Pakistan Journal of Nutrition 16: 645-650.
Melia S, Yuherman, Jaswandi, Purwati E. 2018. Selection of buffalo milk, lactic acid bacteria with probiotic potential. Asian J Pharmaceut Clin Res 11: 186-189.
Melia S, Ferawati, Yuherman, Jaswandi, Purwanto H, Purwati E. 2018. Probiotic characterization of lactic acid bacteria isolated from raw milk (buffallo, cow, and goat) from West Sumatera, Indonesia. Asian Journal Of Microbiology, Biotechnology And Environmental Sciences 20 : S131-S139.
Melia S, Purwati E, Kurnia YF, Pratama DR. 2019. Antimicrobial potential of Pediococcus acidilactici from Bekasam, fermentation of sepat rawa fish (Tricopodus trichopterus) from Banyuasin, South Sumatra, Indonesia. Biodiversitas J 20: 3532-3538.
Menel Z, Sana M, Nedia K, Moktar H, Ali F. 2011. Microbiological analysis and screening of lactic acid bacteria from tunisian date palm sap. African Journal of Microbiology Research 5: 2929-2935.
Mojgani N, Hussaini F, Vaseji N. 2015. Characterization of indigenous Lactobacillus strains for probiotic properties. Jundishapur J. Microbiol 8: 1-6
Nuryady MM, Istiqamah T, Faizah R, Ubaidilah S, Mahmudi Z, Sutoyo. 2013. Isolasi dan identifikasi bakteri asam laktat asal yoghurt. UJEJ Jurnal 1:1-11. [Indonesian]
Pan X, Chen F, Wu T, Tang H, Zhao Z. 2009. The acid, bile tolerance and antimicrobial property of Lactobacillus acidophilus NIT. Food Control 20: 598-602.
Public Health England. 2014. Identification of Staphylococcus Species, Micrococcus Species and Rothia Species. UK Standards for Microbiology Investigations, UK.
Purwandhani SN, Utami T, Milati R, Rahayu ES. 2018. Isolation, characterization and screening of folate-producing bacteria from traditional fermented food (Dadih). International Food Research Journal 25: 566-572.
Rashid S, Hassanshahian M. 2014. Screening, Isolation and Identification of Lactic Acid Bacteria From a Traditional Dairy Product of Sabzevar, Iran. Int J Enteric Pathog. 2: e18393.
Romadhon, Subagiyo, Margino S. 2012. Isolasi dan karakterisasi bakteri asam laktat dari usus udang penghasil bakteriosin sebagai agen antibakteria
pada produk-produk hasil perikanan. Jurnal Saintek Perikanan 8: 59-64. [Indonesian]
Salminen S, Wright AV, Ouwehand A. 2004. Lactic Acid Bacteria: Microbiology and Functional Aspects. 3rd edition. Revised and Expanded
Marcel Dekker Inc, New York.
Sunaryanto R, Marwoto B. 2013. Isolasi, identifikasi, dan karakterisasi bakteri asam laktat dari dadih susu kerbau. Jurnal Sains dan Teknologi Indonesia 14: 228-233. [Indonesian]
Shafakatullah N, Chandra M. 2014. Screening of raw buffalo’s milk from Karnataka for potential probiotic strains. Research Journal of Recent Sciences 3: 73-78.
Syukur, S., Utami L. S., Purwati, E., Urnemi dan Jamsari. 2011. Screening and invitro antimicrobial, protease activities from West Sumatera, Indonesia, Proceedings of the International Seminar on HKI, Pekanbaru, 17-21 Juli 2011. [Indonesian]
Syukur S, Purwati E. 2013. Bioteknologi Probiotik untuk Kesehatan Masyarakat. Penerbit Andi, Yogyakarta. [Indonesian]
Tropcheva R, Nikolova D, Evstatieva Y, Danova S. 2014. Antifungal activity and identification of Lactobacilli, isolated from traditional dairy product “katak”. Anaerobe 28: 78-84.
Unus, 2005. Basic Microbiology. Publisher Papas Sinar Sinanti, Jakarta. [Indonesian]
Vasiee, AR, Tabatabaei YF, Mortazavi A, Edalatian MR. 2014. Isolation, identification and characterization of probiotic Lactobacilli spp. from Tarkhineh. International Food Research Journal 21: 2487-2492.
Yang E, Fan L, Jiang Y, Doucette C, Fillmore S. 2012. Antimicrobial activity of bacteriocin-producing lactic acid bacteria isolated from cheeses and yogurts. AMB Express 2: 48
Yelnetty A, Purnomo, Purwadi, Mirah A. 2014. Biochemical characteristics of lactic acid bacteria with proteolytic activity and capability as starter culture isolated from spontaneous fermented local goat milk. Journal of Natural Sciences Research 4: 137-146.
Zhang B, Wang Y, Tan Z, Li Z, Jiao Z. 2016. Screening of probiotic activities of Lactobacilli strains isolated from traditional tibetan qula, a raw yak milk cheese. Asian Australas J Anim Sci. 29: 1490-1499.
Arena MP, Capozzi V, Spano G, Fiocco D. 2017. The potential of lactic acid bacteria to colonize biotic and abiotic surfaces and the investigation of their interactions and mechanisms. Applied Microbiology and Biotechnology 101: 2641- 2657.
Amezquita A, Brashears MM. 2002. Competitive inhibition of Listeria monocytogenes in ready-to-eat meat products by lactic acid bacteria. Journal of Food Protect 65: 316-325.
Anandharaj M, Sivasankari B. 2014. Isolation of potential probiotic lactobacillus oris HMI68 from mother’s milk with cholesterol-reducing property. Journal of Bioscience and Bioengineering 118: 153-9.
Aritonang SN, Roza E, Rossi E, Purwati E, Husmaini. 2017. Isolation and identification of lactic acid bacteria from okara and evaluation of their potential as candidate probiotics. Pakistan Journal of Nutrition 16: 618-628.
Azat R, Liu Y, Li W, Kayir A. 2016. Probiotic properties of lactic acid bacteria isolated from traditionally fermented Xinjiang cheese. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology) 17: 597-609.
Dallal SMM, Davoodabadi A, Abdi M, Hajiabdolbaghi M, Sharifi Yazdi MK, Douraghi M, Tabatabaei BSM. 2017. Inhibitory effect of Lactobacillus plantarum and Lactobacillus fermentum isolated from the faeces of healthy infants against nonfermentative bacteria causing nosocomial infections. New Microbes and New Infections 15: 9–13.
Food and Agriculture Organization of United Nations & Organization (FAO). 2002. Guidelines for the evaluation of probiotics in food. Report of a Joint FAO/WHO Working Group on Drafting Guidelines for the Evaluation of Probiotics in Food. London, London Ontario.
Harun H, Wirasti Y, Purwanto B, Purwati E. 2020. Characterization of lactic acid bacteria and determination of antimicrobial activity in dadih from Air Dingin Alahan Panjang District, Solok Regency-West Sumatera. Sys Rev Pharm 11: 583-586.
Jain N, Mehta A, Bharti V. 2017. Screening, characterization and in vitro evaluation of probiotic of Lactobacillus strains. Asian Journal of Pharmaceutical and Clinical Research 10: 288-93.
Juliyarsi I, Hartini P, Yuherman, Djamaan A, Arief, Purwanto H, Aritonang SN, Hellyward J, Purwati E. 2018. Characterization of lactic acid bacteria and determination of antimicrobial activity in tempoyak from Padang Pariaman District, West Sumatra, Indonesia. Pakistan Journal of Nutrition 17: 506-511.
Joseph GH, Payung L. 2012. Pengolahan glanular gula dari gula semut aren. Jurnal B. Palma 12: 60-65 [Indonesian]
Kocabay S, Cetinkaya S. 2020. Probiotic properties of Lactobacillus fermentum isolated from new-born faeces. Journal of Oleo Sciensce 12: 1579-1584.
Melia S, Purwati E, Yuherman, Jaswandi, Aritonang SN, Silaen M. 2017. Characterization of the antimicrobial activity of lactic acid bacteria isolated from buffalo milk in West Sumatera (Indonesia) against Listeria monocytogenes. Pakistan Journal of Nutrition 16: 645-650.
Melia S, Yuherman, Jaswandi, Purwati E. 2018. Selection of buffalo milk, lactic acid bacteria with probiotic potential. Asian J Pharmaceut Clin Res 11: 186-189.
Melia S, Ferawati, Yuherman, Jaswandi, Purwanto H, Purwati E. 2018. Probiotic characterization of lactic acid bacteria isolated from raw milk (buffallo, cow, and goat) from West Sumatera, Indonesia. Asian Journal Of Microbiology, Biotechnology And Environmental Sciences 20 : S131-S139.
Melia S, Purwati E, Kurnia YF, Pratama DR. 2019. Antimicrobial potential of Pediococcus acidilactici from Bekasam, fermentation of sepat rawa fish (Tricopodus trichopterus) from Banyuasin, South Sumatra, Indonesia. Biodiversitas J 20: 3532-3538.
Menel Z, Sana M, Nedia K, Moktar H, Ali F. 2011. Microbiological analysis and screening of lactic acid bacteria from tunisian date palm sap. African Journal of Microbiology Research 5: 2929-2935.
Mojgani N, Hussaini F, Vaseji N. 2015. Characterization of indigenous Lactobacillus strains for probiotic properties. Jundishapur J. Microbiol 8: 1-6
Nuryady MM, Istiqamah T, Faizah R, Ubaidilah S, Mahmudi Z, Sutoyo. 2013. Isolasi dan identifikasi bakteri asam laktat asal yoghurt. UJEJ Jurnal 1:1-11. [Indonesian]
Pan X, Chen F, Wu T, Tang H, Zhao Z. 2009. The acid, bile tolerance and antimicrobial property of Lactobacillus acidophilus NIT. Food Control 20: 598-602.
Public Health England. 2014. Identification of Staphylococcus Species, Micrococcus Species and Rothia Species. UK Standards for Microbiology Investigations, UK.
Purwandhani SN, Utami T, Milati R, Rahayu ES. 2018. Isolation, characterization and screening of folate-producing bacteria from traditional fermented food (Dadih). International Food Research Journal 25: 566-572.
Rashid S, Hassanshahian M. 2014. Screening, Isolation and Identification of Lactic Acid Bacteria From a Traditional Dairy Product of Sabzevar, Iran. Int J Enteric Pathog. 2: e18393.
Romadhon, Subagiyo, Margino S. 2012. Isolasi dan karakterisasi bakteri asam laktat dari usus udang penghasil bakteriosin sebagai agen antibakteria
pada produk-produk hasil perikanan. Jurnal Saintek Perikanan 8: 59-64. [Indonesian]
Salminen S, Wright AV, Ouwehand A. 2004. Lactic Acid Bacteria: Microbiology and Functional Aspects. 3rd edition. Revised and Expanded
Marcel Dekker Inc, New York.
Sunaryanto R, Marwoto B. 2013. Isolasi, identifikasi, dan karakterisasi bakteri asam laktat dari dadih susu kerbau. Jurnal Sains dan Teknologi Indonesia 14: 228-233. [Indonesian]
Shafakatullah N, Chandra M. 2014. Screening of raw buffalo’s milk from Karnataka for potential probiotic strains. Research Journal of Recent Sciences 3: 73-78.
Syukur, S., Utami L. S., Purwati, E., Urnemi dan Jamsari. 2011. Screening and invitro antimicrobial, protease activities from West Sumatera, Indonesia, Proceedings of the International Seminar on HKI, Pekanbaru, 17-21 Juli 2011. [Indonesian]
Syukur S, Purwati E. 2013. Bioteknologi Probiotik untuk Kesehatan Masyarakat. Penerbit Andi, Yogyakarta. [Indonesian]
Tropcheva R, Nikolova D, Evstatieva Y, Danova S. 2014. Antifungal activity and identification of Lactobacilli, isolated from traditional dairy product “katak”. Anaerobe 28: 78-84.
Unus, 2005. Basic Microbiology. Publisher Papas Sinar Sinanti, Jakarta. [Indonesian]
Vasiee, AR, Tabatabaei YF, Mortazavi A, Edalatian MR. 2014. Isolation, identification and characterization of probiotic Lactobacilli spp. from Tarkhineh. International Food Research Journal 21: 2487-2492.
Yang E, Fan L, Jiang Y, Doucette C, Fillmore S. 2012. Antimicrobial activity of bacteriocin-producing lactic acid bacteria isolated from cheeses and yogurts. AMB Express 2: 48
Yelnetty A, Purnomo, Purwadi, Mirah A. 2014. Biochemical characteristics of lactic acid bacteria with proteolytic activity and capability as starter culture isolated from spontaneous fermented local goat milk. Journal of Natural Sciences Research 4: 137-146.
Zhang B, Wang Y, Tan Z, Li Z, Jiao Z. 2016. Screening of probiotic activities of Lactobacilli strains isolated from traditional tibetan qula, a raw yak milk cheese. Asian Australas J Anim Sci. 29: 1490-1499.
Most read articles by the same author(s)
- SRI MELIA, ENDANG PURWATI, YULIANTI FITRI KURNIA, DHIVA REZZY PRATAMA, Antimicrobial potential of Pediococcus acidilactici from Bekasam, fermentation of sepat rawa fish (Tricopodus trichopterus) from Banyuasin, South Sumatra, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 20 No. 12 (2019)
- SRI MELIA, INDRI JULIYARSI, YULIANTI FITRI KURNIA, YUDHA ENDRA PRATAMA, DHIVA REZZY PRATAMA, The quality of fermented goat milk produced by Pediococcus acidilactici BK01 on refrigerator temperature , Biodiversitas Journal of Biological Diversity: Vol. 21 No. 10 (2020)
- SUMARYATI SYUKUR, SAFRIZAYANTI, SITI ZULAIHA, EPI SUPRIWARDI, AULIA FAHMI, KUNTUM KH NURFADILAH, ENDANG PURWATI, Potential antioxidant activity of Lactobacillus fermentum KF7 from virgin coconut oil products in Padang, West Sumatra, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 23 No. 3 (2022)
- SRI MELIA, SALAM N. ARITONANG, INDRI JULIYARSI, YULIANTI FITRI KURNIA, RUSDIMANSYAH, VIRA OKTAVIA HERNITA, The screening of probiotic lactic acid bacteria from honey of stingless bee from West Sumatra, Indonesia and using as starter culture , Biodiversitas Journal of Biological Diversity: Vol. 23 No. 12 (2022)
- ALIYAH FAHMI, SUMARYATI SYUKUR, ZULKARNAIN CHAIDIR, SRI MELIA, Isolation and molecular characterization of probiotic from Sidamanik green tea (Camellia sinensis) fermentation , Biodiversitas Journal of Biological Diversity: Vol. 24 No. 8 (2023)
- SUSMIATI, SRI MELIA, ILFA KHAIRINA, HURRIYA ALZAHRA, Changes in physicochemical, microbiological, and sensory quality of fermented milk with Lactiplantibacillus pentosus during cold storage , Biodiversitas Journal of Biological Diversity: Vol. 25 No. 1 (2024)
- SUSMIATI, SRI MELIA, ENDANG PURWATI, HURRIYA ALZAHRA, Physicochemical and microbiological fermented buffalo milk produced by probiotic Lactiplantibacillus pentosus HBUAS53657 and sweet orange juice (Citrus nobilis) , Biodiversitas Journal of Biological Diversity: Vol. 23 No. 8 (2022)
- ARI HARMAYANI, MARLINA, ENDANG PURWATI, Characteristics of probiotic lactic acid bacteria isolated from edible birds nests as antimicrobials potential , Biodiversitas Journal of Biological Diversity: Vol. 25 No. 4 (2024)