Antibacterial activity of lactic acid bacteria isolated from Dengke Naniura of Carp (Cyprinus carpio) against diarrhea-causing pathogenic bacteria
##plugins.themes.bootstrap3.article.sidebar##
Issue
Section
Articles
##plugins.themes.bootstrap3.article.main##
Abstract
Abstract. Nasri, Harahap U, Silalahi J, Satria D. 2021. Antibacterial activity of lactic acid bacteria isolated from Dengke Naniura of Carp (Cyprinus carpio) against diarrhea-causing pathogenic bacteria. Biodiversitas 22: 3098-3104. Diarrhea is the discharge of liquid or watery stools 3 to 4 times a day caused by a bacterial infection. Treatments for diarrhea are probiotics, which have a beneficial effect on the health of the host such as antibacterial. Traditional Batak Toba fermented food, Dengke Naniura, is a source of probiotics. This study aimed to determine the minimum inhibitory concentration, minimum bactericidal concentration, and leakage of DNA and protein from lactic acid bacteria against pathogens. Isolation of LAB was obtained from Dengke Naniura by pour plate method on deMann Rogosa and Sharpe Agar + CaCO3 1%. In this study, Characterization and analysis of bacterial sequencing used Polymerase Chain Reaction. Determination of MIC used the agar diffusion method. The MBC test used the streaking method which was a stroke from the inhibition zone formed. DNA and protein leakage was measured using spectrophotometry UV-VIS (260nm and 280nm). The isolation results obtained were Lactobacillus fermentum, the characterization showed that the bacteria were Gram-positive, bacilli, non-sporing, catalase-negative, and able to ferment sugar. The MIC determination was obtained at a concentration of 10%v/v with a clear zone diameter. Determination of MBC against pathogens was obtained at different concentrations. The results of DNA and protein leakage showed an increased absorption (260nm and 280nm).
##plugins.themes.bootstrap3.article.details##
References
Ashakirin, S. N., Tripathy, M., Patil, U. K., & Majeed, A. B. A. (2017). ANTIMICROBIAL ACTIVITY OF ESSENTIAL OILS: EXPLORATION ON MECHANISM OF BIOACTIVITY. International Journal of Pharmaceutical Sciences and Research, 8(8), 3187–3193. https://doi.org/10.13040/IJPSR.0975-8232.8(8).3187-93
Asriani, Laksmi, B. S., Yasni, S., & Sudirman, I. (2007). Mekanisme antibakteri metabolit Lb. plantarum kik dan monoasilgliserol minyak kelapa terhadap bakteri patogen pangan. In Jurnal Teknologi dan Industri Pangan (Vol. 18, Issue 2, pp. 126–132).
Balouiri, M., Sadiki, M., & Ibnsouda, S. K. (2016). Methods for in vitro evaluating antimicrobial activity: A review. Journal of Pharmaceutical Analysis, 6(2), 71–79. https://doi.org/10.1016/j.jpha.2015.11.005
Chen, C. C., Lai, C. C., Huang, H. L., Huang, W. Y., Toh, H. S., Weng, T. C., Chuang, Y. C., Lu, Y. C., & Tang, H. J. (2019). Antimicrobial activity of lactobacillus species against carbapenem-resistant enterobacteriaceae. Frontiers in Microbiology, 10(APR), 1–10. https://doi.org/10.3389/fmicb.2019.00789
Davis, W. W., & Stout, T. R. (1971). Disc plate method of microbiological antibiotic assay. II. Novel procedure offering improved accuracy. Applied Microbiology, 22(4), 666–670. https://doi.org/10.1128/aem.22.4.666-670.1971
Fitri, D. S., Pangastuti, A., Susilowati, A., & Sutarno. (2017). Endophytic bacteria producing antibacterial against methicillin-resistant staphylococcus aureus (MRSA) in seagrass from Rote Ndao, East Nusa Tenggara, Indonesia. Biodiversitas, 18(2), 733–740. https://doi.org/10.13057/biodiv/d180242
Haro, G., Iksen, I., & Nasri, N. (2020). Identification, characterization and antibacterial potential of probiotic lactic acid bacteria isolated from naniura (A traditional batak fermented food from carp) against Salmonella typhi. Rasayan Journal of Chemistry, 13(1), 464–468. https://doi.org/10.31788/RJC.2020.1315530
Huang, J., Qian, C., Xu, H., & Huang, Y. (2018). Antibacterial activity of Artemisia asiatica essential oil against some common respiratory infection causing bacterial strains and its mechanism of action in Haemophilus influenzae. Microbial Pathogenesis, 114(January), 470–475.
Hutahaean, A. J. N., Silalahi, J., Suryanto, D., & Satria, D. (2019). Characterisation of lactic acid bacteria from Dengke Naniura of common carp (Cyprinus carpio) with ?-glucosidase inhibitory activity. Open Access Macedonian Journal of Medical Sciences, 7(22), 3794–3798. https://doi.org/10.3889/oamjms.2019.506
Ismail, Y. S., Yulvizar, C., & Putriani. (2017). Isolasi, karakterisasi dan uji aktivitas antimikroba bakteri asam laktat dari fermentasi biji kakao (Theobroma cacao L.). Jurnal Bioleuser, 1(2), 45–53.
Kawthar, M. A., Hanan, B. E., Yousif, F. H. E., & Ahmed, E. E. (2018). Molecular characterization of lactic acid bacteria isolated from starter dough of Sudanese sorghum fermented flat bread (Kissra). Pakistan Journal of Nutrition, 17(2), 57–63. https://doi.org/10.3923/pjn.2018.57.63
Klindworth, A., Pruesse, E., Schweer, T., Peplies, J., Quast, C., Horn, M., & Glöckner, F. O. (2013). Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencing-based diversity studies. Nucleic Acids Research, 41(1), 1–11. https://doi.org/10.1093/nar/gks808
Kuspradini, H., Putri, A. S., Egra, S., & Yanti. (2019). Short communication: In vitro antibacterial activity of essential oils from twelve aromatic plants from East Kalimantan, Indonesia. Biodiversitas, 20(7), 2039–2042. https://doi.org/10.13057/biodiv/d200733
Lin, C. M., Preston, J. F., & Wei, C. I. (2000). Antibacterial mechanism of allyl isothiocyanate. Journal of Food Protection, 63(6), 727–734. https://doi.org/10.4315/0362-028X-63.6.727
Manik, M., Kaban, J., Silalahi, J., & Ginting, M. (2021). Lactic acid bacteria (LAB) with probiotic potential from Dengke Naniura. Baghdad Science Journal, 18(1), 35–40. https://doi.org/10.21123/bsj.2021.18.1.0035
Mierza, V., Rosidah, Haro, G., & Suryanto, D. (2020). Antibacterial activity and mechanism of action of rarugadong (Dioscorea pyrifolia kunth.) tuber extracts on escherichia coli and staphylococcus aureus cell leakage. Rasayan Journal of Chemistry, 13(3), 1894–1903. https://doi.org/10.31788/RJC.2020.1335864
Mostafa, A. A., Al-Askar, A. A., Almaary, K. S., Dawoud, T. M., Sholkamy, E. N., & Bakri, M. M. (2018). Antimicrobial activity of some plant extracts against bacterial strains causing food poisoning diseases. Saudi Journal of Biological Sciences, 25(2), 361–366. https://doi.org/10.1016/j.sjbs.2017.02.004
Ouchari, L., Boukeskasse, A., Bouizgarne, B., & Ouhdouch, Y. (2019). Antimicrobial potential of actinomycetes isolated from the unexplored hot Merzouga desert and their taxonomic diversity. Biology Open, 8(2). https://doi.org/10.1242/bio.035410
Owusu-Kwarteng, J., Tano-Debrah, K., Akabanda, F., & Jespersen, L. (2015). Technological properties and probiotic potential of Lactobacillus fermentum strains isolated from West African fermented millet dough Applied microbiology. BMC Microbiology, 15(1), 1–10. https://doi.org/10.1186/s12866-015-0602-6
Sanyaolu, A., Okorie, C., Marinkovic, A., Jaferi, U., & Prakash, S. (2020). Global Epidemiology and Management of Acute Diarrhea in Children from Developing Countries. Ann Pediatr Child Health, 8(8), 1205.
Sari, D. P., & Budyanra, B. (2017). The Risk Factor that Affect Children Diarrhea in The Island of Java 2013 (Riskesdas 2013 Data Analysis). Journal of Educational, Health and Community Psychology, 6(1), 1. https://doi.org/10.12928/jehcp.v6i1.6615
Tejan, N., Datta, P., & Gupta, V. (2018). Bacterial Diarrhoea: a Comprehensive Review. International Journal of Pharmaceutical Sciences and Research, 9(12), 5015–5031. https://doi.org/10.13040/IJPSR.0975-8232.9(12).5015-31
Ugboko, H. U., Nwinyi, O. C., Oranusi, S. U., & Oyewale, J. O. (2020). Childhood diarrhoeal diseases in developing countries. Heliyon, 6(4), e03690. https://doi.org/10.1016/j.heliyon.2020.e03690
Yang, J. H., Wu, U. I., Tai, H. M., & Sheng, W. H. (2019). Effectiveness of an ultraviolet-C disinfection system for reduction of healthcare-associated pathogens. Journal of Microbiology, Immunology and Infection, 52(3), 487–493. https://doi.org/10.1016/j.jmii.2017.08.017
Most read articles by the same author(s)
- Ridwanti Batubara, BASUKI WIRJOSENTONO, AMIR HAMZAH SIREGAR, URIP HARAHAP, TAMRIN TAMRIN, Bioactive compounds of ethanol extract from agarwood leaves (Aquilaria malaccensis) and antimicrobial activity against bacteria and fungi growing on the skin , Biodiversitas Journal of Biological Diversity: Vol. 22 No. 5 (2021)
- PUTU SUPADMA PUTRA, TOSHIHIRO YAMADA, AMRAN ACHMAD, NASRI , ANDI SIADY HAMZAH, PUTU OKA NGAKAN, Differences in the vegetation dynamic patterns of three tropical secondary forests in South Sulawesi, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 24 No. 9 (2023)
- IIN PERTIWI A. HUSAINI, RISMA ILLA MAULANY, NASRI NASRI, PUTU OKA NGAKAN, Diversity and use of traditional medicinal plant species in Bantimurung-Bulusaraung National Park, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 23 No. 11 (2022)