Short Communication: Isolation and screening of polyhydroxylalkanoates producing microorganisms from Thailand

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DOI https://doi.org/10.13057/biodiv/d221131
Issue
Vol. 22 No. 11 (2021)
Section
Articles
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This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

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NUNTAPORN PUNGSUNGVORN ♥
Department of Biotechnology, Faculty of Science and Technology, Rajamangala University of Technology Tawan-ok. 43 Moo 6, Bang Phra, Sriracha, Chonburi 20110, Thailand
ANUSSORN WISETSING
Department of Biotechnology, Faculty of Science and Technology, Rajamangala University of Technology Tawan-ok. 43 Moo 6, Bang Phra, Sriracha, Chonburi 20110, Thailand

Abstract

Abstract. Pungsungvorn N, Wisetsing A. 2021. Short Communication: Isolation and screening of polyhydroxylalkanoates producing microorganisms from Thailand. Biodiversitas 22: 4963-4967. Polyhydroxylalkanotes (PHAs) are polyesters produced in nature by numerous microorganisms. They are biodegradable and are used in the production of bioplastics. In this study, one hundred twenty samples from different regions of Thailand were collected and screened for polyhydroxylalkanoates (PHAs) producing microorganisms. The samples were screened on YM agar containing 0.5 µg Nile-red/mL (YM-NR). Only one isolate of yeast (Y1) gave a positive result on PHA accumulation. The yeast isolate (Y1) was identified as Candida tropicalis by API 20C AUX kit and 18S rRNA nucleotide comparison. The yeast isolate Y1 produced 2.62% PHA   when grown in synthetic N-limiting medium using rice straw hydrolysate as carbon source. The selected four bacteria (B1, B2, B3 and B4) were identified by BlastN of 16S rRNA as Enterobacter cloacae, Enterobacter carcerogenus, Escherichia coli and Klebsiella pneumonia, respectively. The selected yeast and bacterial strains gave PHA content of 2.62, 2.76, 5.38, 3.66 and 0.44%, respectively, in synthetic N-limiting medium using rice straw hydrolysate. Hence, these microorganisms could be used in PHA production from biomass in the future.

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References
Aba-El-Haleem, DAM, Zaki SA, Abuelhamd AT, Amara A, Aboelreesh GMS. 2007. Biosynthesis of polyhydroxyalkanotes. J Appl Sci Environ Manage. 11:5-10.
Byrom D. 1987. Polymer synthesis by microorganisms: technology and economics. Trend Biotech. 5:246-250.
Castilho LR, Mitchell DA, Freire DMG. 2009. Production of polyhydroxyalkanoates (PHAs) from waste materials and byproducts by submerged and solid-state fermentation. Biores Technol. 100:5996–6009.
Ceyhan N, Ozdemir G. 2011. Poly-????-hydroxybutyrate (PHB) production from domestic wastewater using Enterobacter aerogenes 12Bi strain. African J Micro Res. 5:690-702.
Cho K, Ryu HW, Park C, Goodrich PR. 1997. Poly(hydroxybutyrate-co-hydroxyvalerate) from swine waste liquor by Azotobacter vinelandii UWD. Biotech Lett.19:7-10.
Choi J, Lee SY. 1997. Process analysis and economic evaluation for poly(3-hydroxybutyrate) production by fermentation. Biopro Eng. 17:335–342.
Desouky AM, Haleem AE, AlMa'adeed MA, Al-Thani N. 2007. Physical and chemical properties of polyhydroxyalkanotes biodegradable polymers produced in transgenic yeasts. Glob J Env Res. 2:69-73.
Desouky AM, Haleem AE. 2009. Biosynthesis of polyhydroxyalkanotes in wild yeasts. Pol J Micro. 58:37-41.
Dias JM, Lemos PC, Serafim LS, Oliveira C, Eiroa M, Albuquerque MG, Ramos AM, Oliveira R, Reis MA. 2006. Recent advances in polyhydroxyalkanoate production by mixed aerobic cultures:from the substrate to the final product. Macromol Biosci. 6:885-906.
Feng X, Xian M, Liu W, Xu C, Zhang H, Zhao G. 2015. Biosynthesis of poly(3-hydroxypropionate) from glycerol using engineered Klebsiella pneumoniae strain without vitamin B12. Bioengineered. 6:77-81.
Grothe E, Moo-Young M, Chisti Y. 1999. Fermentation optimization for the production of poly(????-hydroxybutyric acid)
microbial thermoplastic. Enz and Microl Tech. 25:132–141.
Halami PM. 2008. Production of polyhydroxyalkanoate fromstarch by the native isolate Bacillus cereus CFR06. World J Micro Biotech. 24:805-812.
Haywood GW, Anderson AJ, Dawes EA. 1989. A survey of the accumulation of novel polyhydroxyalkanoates by bacteria. Biotech Lett. 11:471-476.
Law JH, Slepecky RA. 1961. Assay of Poly-?-Hydroxybutyric Acid. J Bacteriol. 82:33-36.
Madison LL, Huisman GW. 1999. Metabolic engineering of poly(3-hydroxyalkanoates): from DNA to plastic. Micro Mol Bio Rev. 63:21-53.
Mart?´nez-Gutie´rrez CA, Latisnere-Barraga´n H, Garc?´a-Maldonado JQ, Lo´pez-Corte´s A. 2018. Screening of polyhydroxyalkanoate-producing bacteria and PhaC-encoding genes in two hypersaline microbial mats from Guerrero Negro, Baja California Sur, Mexico. PeerJ 6:e4780; DOI 10.7717/peerj.4780
Pirozzi, D, Ausiello A, Yousuf A, Zuccaro G and Toscano G. 2014. Exploitation of oleaginious yeasts for the production of microbial oils from agricultural biomass. Chem Eng Trans. 37:469-474.
Qi, Q, Steinbuchel A, Rehm BHA. 1998. Metabolic routing towards polyhydroxyalkanoic acid synthesis in recombinant Escherichia coli (fadR): inhibition of fatty acid ?-oxidation by acrylic acid. FEMS Micro Lett. 167:89-94.
Rehm BHA. 2003. Polyester synthases: natural catalysts for plastics. Biochem J. 376:15-33.
Rudnik E. 2008. Compostable Polymer Materials. Elsevier, Amsterdam.
Salehizadeh H, van Loosdrecht MCM. 2004. Production of polyhydroxyalkanoates by mixed culture: recent trends and
biotechnological importance. Biotech Adv. 22:261-279.
Samrot AV, Avinesh RB, Sukeetha SD, Senthilkumar P. 2011. Accumulation of poly [(R)-3-hydroxyalkanoates] in Enterobacter cloacae SU-1 during growth with two different carbon sources in batch culture. Appl.Biochem Biotechnol. 163:195-203.
Spiekermann P, Rehm BHA, Kalscheuer R, Baumeister D, Steinbuchel A. 1999. A sensitivity, viable-colony staining method using Nile red for direct screening of bacteria that accumulate polyhydroxyalkanoic acids and other lipid storage compounds. Arch. Microbiol. 171:73-80.
Tamer IM, Moo-Young M, Chisti Y.1998. Optimization of poly(????-hydroxybutyric acid) recovery from Alcaligenes latus:
combined mechanical and chemical treatments. Biopro Eng. 19:459-468.
Thammasittirong A, Saechow S, Na-Ranong Thammasittirong S. 2017. Efficient polyhydroxybutyrate production from Bacillus thuringiensis using sugarcane juice substrate. Turk J Biol. 41: 992-1002.
Yamane T. 1992. Cultivation engineering of microbial bioplastics production. FEMS Micro Rev. 103:257-264.
Yamane T. 1993. Yield of poly-D(?)-3-hydroxybutyrate from various carbon sources: a theoretical study. Biotech Bioeng. 41:165-170.
Yu J. 2001. Production of PHA from starchy wastewater via organic acids. J Biotech. 86:105-112.