Abstract. Suroto DA, Hasan PN, Rahayu ES. 2021. Genomic insight of two indigenous probiotics Lactobacillus plantarum Dad-13 and Lactobacillus plantarum Mut-7 from different origins of Indonesian fermented foods. Biodiversitas 22: 5491-5500. Lactobacillus plantarum Dad-13 and Lactobacillus plantarum Mut-7 are probiotics isolated from traditional fermented foods. In the present study two strains of L. plantarum were sequenced using Illumina NovaSeq PE150 platform and in silico analysis was performed using RAST webserver to unravel the functional metabolic between two strains. The L. plantarum Dad-13 and Mut-7 genome sizes were 3.25 and 3.98 Mb with GC content of them being almost similar i.e. 44.4 and 44.3%, respectively. Two lactobacilli also had some differences in their metabolic genes, particularly in carbohydrate subsystem. L. plantarum Mut-7 had genes related to the metabolisms of plant cell wall components such as dextrin, arabinose, xylose indicated the adaptation to their environmental origins (fermented dried cassava), while those genes were not observed in L. plantarum Dad-13 which was isolated from fermented buffalo’s milk (dadih). Further analysis using BAGEL4 webserver exhibited their different potential to synthesize bacteriocins, L. plantarum Dad-13 was predicted to produce more types of bacteriocins than L. plantarum Mut-7. This investigation revealed that L. plantarum Dad-13 and L. plantarum Mut-7 have a great potential related with their properties as probiotics.