Identification of compounds in red fruit (Pandanus conoideus) seed ethanol extract

Abstract

Sundari, Wibowo FR. 2015. Identification of compounds in red fruit (Pandanus conoideus) seed ethanol extract. Biofarmasi 13: 78-89. Identification of polar compound in red fruit seed has been done. Non- polar compound was isolated by soxhlet method using petroleum ether. Then polar compound was isolated from residues of red fruit seed soxhletation by maceration method using ethanol 96%. As much as 10.953 g of concentrate ethanol extract was yielded from 150 g dry powder red fruit seed. Compound of red fruit seed ethanol extract was identified by phytochemical screening. The result showed that ethanol extract contains tannin, anthraquinone glycoside, phenolic compound glycoside, and fatty acid glycoside. Then ethanol extract was separated by various separation methods, included flash column chromatography, Sephadex LH-20 column chromatography, and HPLC. Flash column chromatography was not able to separate all the polar compound of red fruit seed. Chromatogram profile of HPLC showed that ethanol extract has minimal 5 compounds. Sephadex chromatography separation was considered the most effective than another separation method because it is able to separate all compounds on ethanol extract of red fruit seeds. The result of Sephadex chromatography separation showed that phenolic glycosides dominate polar component of red fruit seeds (57.375%), followed by tannin (22.345%) and anthraquinone glycoside (18.732%). In general, the results of this study indicated that compounds of ethanol extract have potential as a drug because tannins and glycosides (anthraquinone and phenolic compounds) are generally antioxidant compounds.