Phytochemical screening and GC-MS analysis of bioactive compounds of Blumea balsamifera leaf extracts from South Aceh, Indonesia




Abstract. Masyudi, Hanafiah M, Rinidar, Usman S, Marlina. 2022. Phytochemical screening and GC-MS analysis of bioactive compounds of Blumea balsamifera leaf extracts from South Aceh, Indonesia. Biodiversitas 23: 1344-1352. Blumea balsamifera is a traditional herbal medicine that has been used worldwide. It is also known as Capa leaf in Aceh, Indonesia. B. balsamifera has been used by the local community for wound healing, anti-bacterial, and anti-inflammation. However, there is no study so far attempting to identify the chemical compounds of B. balsamifera leaves from Aceh. The present study thus aimed to analyze the chemical compounds of B. balsamifera leaves from Aceh. In this study, the B. balsamifera leaves were collected from Gunongpulo village, South Aceh, Indonesia. The extraction process was performed using the maceration method. The active chemical compounds in the B. balsamifera leaf extracts with n-hexane, ethyl acetate, and ethanol solvents were analyzed through phytochemical screening, Fourier Transform Infrared (FTIR) Spectroscopy Analysis and GC-MS. The results showed that B. balsamifera leaf extracts with ethanol and ethyl acetate solvents contain saponins, flavonoids, phenolics, tannins, and steroids, identified through the phytochemical screening. Meanwhile, the extract of B. balsamifera leaves with n-hexane solvent was found to contain steroids, phenolics, and tannins. Twenty-four chemical compounds in the B. balsamifera leaf extract with ethanol were identified using GC-MS. Twenty-eight and twenty-seven chemical compounds were also identified in the B. balsamifera leaf extracts with ethyl acetate and n-hexane solvents, respectively. The most abundant compounds found in the B. balsamifera leaf extracts with ethanol, ethyl acetate, and n-hexane solvents were 2,5-Dimethoxyacetophenone (11.61%), Borneol (14.48%), and Jasmoline (14.32%), respectively. These compounds are members of the flavonoid group which are effective for antibacterial, anti-inflammation, and wound healing applications.


Abu-Rabia, A. 2005. Urinary diseases and ethnobotany among pastoral nomads in the Middle East. Journal of Ethnobiology and Ethnomedicine I (4) : 1-4. doi:10.1186/1746-4269-1-4
Asmilia, N., Fahrimal, Y., Abrar, M., & Rinidar, R. (2020). Chemical Compounds of Malacca Leaf (Phyllanthus emblica) after Triple Extraction with N-Hexane, Ethyl Acetate, and Ethanol. The Scientific World Journal, 2020 (1): 1-5, article id 2739056. doi: 10.1155/2020/2739056
Chen, M., Qin, J.-J., Fu, J.-J., Hu, X.-J., Liu, X.-H., Zhang, W.-D., & Jin, H.-Z. (2010). Blumeaenes A–J , Sesquiterpenoid Esters from Blumea balsamifera with NO Inhibitory Activity. Planta Medica, 76 (9): 897–902.
Chu, S. S., Du, S. S., & Liu, Z. L. (2013). Fumigant Compounds from the Essential Oil of Chinese Blumea balsamifera Leaves against the Maize Weevil (Sitophilus zeamais). Journal of Chemistry, 2013 (1):1-7, 289874. doi: 10.1155/2013/289874
Cowan, M. M. (1999). Plant Products as Antimicrobial Agents. Clinical Microbiology Reviews, 12(4): 564–582,
Dachriyanus. (2004). Analisis Struktur Senyawa Organik Secara Spektroskopi. Book LPTIK Andalas University, Padang, Indonesia
De, S., Dey, A., Babu, A. M. S. S., & Aneela, S. (2013). Phytochemical and GC-MS analysis of bioactive compounds of Sphaeranthus amaranthoides Burm. Pharmacognosy Journal, 5(6): 265–268.
Ekor, M. (2014). The growing use of herbal medicines: issues relating to adverse reactions and challenges in monitoring safety. Frontiers in Pharmacology, 4, 177 : 1-10. doi: 10.3389/fphar.2013.00177
Hariharan, P., & Subburaju, T. (2012). Medicinal Plants And Its Standardization – A Global And Industrial Overview. Global Journal of Medicinal Plant Research, 1(1), 10–13.
Hussein, A., Hussein, R. A., & El-anssary, A. A. (2018). Plants Secondary Metabolites?: The Key Drivers of the Pharmacological Pharmacological Actions Actions of of Medicinal Medicinal Plants Plants Rehab. IntechOpen.
Hussein, R. A., & El-Anssary, A. A. (2018). Plants Secondary Metabolites: The Key Drivers of the Pharmacological Actions of Medicinal Plants. In Herbal Medicine (pp. 11–30). IntechOpen.
Igoli, J. O., Ogaji, O. G., Tor-Anyiin, T. A., & Igoli, N. P. (2005). Traditional Medicine Practice Amongst the Igede People of Nigeria. Part II. African Journal of Traditional, Complementary, and Alternative Medicines, 2(2), 134–152.
Kavitha, R. (2021). Phytochemical Screening and GC-MS Analysis of Bioactive Compounds Present in Ethanolic Extracts of Leaf and Fruit of Trichosanthesis Dioica Roxb. International Journal of Pharmaceutical Sciences and Research, 12(5), 2755–2764. doi: 10.13040/IJPSR.0975-8232
Kumar, R. S., Venkateshwar, C., Samuel, G., & Rao, S. G. (2013). Phytochemical Screening of some compounds from plant leaf extracts of Holoptelea integrifolia (Planch.) and Celestrus emarginata (Grah.) used by Gondu tribes at Adilabad District, Andhrapradesh, India. International Journal of Engineering Science Invention, 2(8), 65–70.
Muttakin, & Zulfajri, M. (2020). Antioxidant Activity of Syzygium cumini Fruit Peel Extract for Diabetes Mellitus Treatment in Alloxan- Induced Diabetic Rats. Research Journal of Chemistry and Environment, 24(1), 9–13.
Pang, Y., Wang, D., Fan, Z., Chen, X., Yu, F., Hu, X., Wang, K., & Yuan, L. (2014). Blumea balsamifera—A Phytochemical and Pharmacological Review. Molecules, 19 (7) : 9453–9477.
Pang, Y., Zhang, Y., Huang, L., Xu, L., Wang, K., & Wang, D. (2017). Effects and Mechanisms of Total Flavonoids from Blumea balsamifera ( L .) DC . on Skin Wound in Rats. International Journal of Moleculer Science, 18 (12): 2766.
Sakee, U., Maneerat, S., Cushnie, T. P. T., & De-eknamkul, W. (2011). Formerly Natural Product Letters Antimicrobial activity of Blumea balsamifera (Lin.) DC. extracts and essential oil. Natural Product Research, 25(19): 1849-1856. doi: 10.1080/14786419.2010.485573
Shirota, O., Oribello, J. M., Sekita, S., & Satake, M. (2011). Sesquiterpenes from Blumea balsamifera. Journal of Natural Products, 74(3): 470-476.
Singh, A., Kumar, A., & Srivastava, V. (2018). Extraction of bioactive compound from different medicinal plants and rhizospheric soil bacteria and their comparative study against human pathogens. Journal of Pharmacognosy and Phytochemistry, 7(6): 528–533.
Thillaivanan, S., & Samraj, K. (2014b). Challenges, Constraints and Opportunities in Herbal Medicines – A Review. International Journal of Herbal Medicine, 2(1), 21–24.
Umer, S., Tekewe, A., & Kebede, N. (2013). Antidiarrhoeal and antimicrobial activity of Calpurnia aurea leaf extract. BMC Complementary and Alternative Medicine, 13(1): 21.
Usaizan, N., Abdullah, N. A. P., Ahmad, S. H., & Saleh, G. (2014). Preliminary Phytochemical Screening and GC-MS Analysis of Ethanol Extract of Physalis Minima L . (Solanaceae). Journal of Advanced Agricultural Technologies, 1(2), 100–103. doi: 10.12720/joaat.1.2.100-103
Valliammai, S., Ashwin, M. D., & Sivachandran, B. (2020). Evaluation of pharmacognostical, phytochemical and antimicrobial properties of Clerodendrum heterophyllum (Poir.) Materials Today: Proceedings, 33(7), 4377–4384.
Welz, A. N., Emberger-Klein, A., & Menrad, K. (2018). Why people use herbal medicine: insights from a focus-group study in Germany. BMC Complementary and Alternative Medicine, 18(1): 92.
Yuan, H., Ma, Q., Ye, L., & Piao, G. (2016a). The Traditional Medicine and Modern Medicine from Natural Products. Molecules, 21(5), 559.

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