Antibacterial activity of Blumea balsamifera leaf extracts from Aceh, Indonesia

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MASYUDI
ANITA NOVIYANTI
MUHAMMAD RIDHWAN
SALFAUQI NURMAN
JAILANI
ARMI
T. M. RAFSANJANI
SAID USMAN
MUHAMMAD HANAFIAH
MARLINA

Abstract

Abstract. Masyudi, Noviyanti A, Ridhwan M, Nurman S, Jailani, Armi, Rafsanjani TM, Usman S, Hanafiah M, Marlina. 2023. Antibacterial activity of Blumea balsamifera leaf extracts from Aceh, Indonesia. Biodiversitas 24: 4584-4589. Blumea balsamifera L. has long been used as traditional medicine by communities in Aceh, Indonesia and from various parts of the world. For generations, this plant has been believed to have properties to cure various diseases. The results of the GCMS analysis of B. balsamifera leaf from Aceh-Indonesia in the previous study contain various compounds for wound healing, including borneol, jasmoline, camphor, and caryophyllene. The present study aimed to analyze the antibacterial activity of B. balsamifera leaves from Aceh. The sample was taken from the village of Gunongpulo, South Aceh-Indonesia, and the extraction process was carried out using three different types of solvents: ethanol, ethyl acetate, and n-hexane. Three variations of the extract obtained were then tested for antibacterial against Staphylococcus aureus and Pseudomonas aeruginosa by analyzing the inhibitory of each extract. The results showed that the inhibition zone formed on S. aureus against ethanol extract was 2.4 mm, ethyl extract was 9.2 mm, and n-hexane extract was 2.3 mm. In comparison, the inhibition zone formed on P. aeruginosa against ethanol extract was 0 mm, ethyl extract was 9 mm, and n-hexane extract was 4.9 mm. Conclusion: B. balsamifera leaf extract from Aceh contains antibacterial, with the highest inhibition against S. aureus at 9.2 mm and Pseudomonas at 9.0 mm. It is recommended that in the process of extracting B. balsamifera leaves by maceration, the best solvent used is ethyl acetate.

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References
Asif, M. et al. (2017) ‘Ethnobotanical and phytopharmacological attributes of Mesua ferrea: A mini review’, Journal of Applied Pharmaceutical Science, 7(4), pp. 242–251. doi: 10.7324/JAPS.2017.70435.
Asmilia, N. et al. (2020) ‘Chemical Compounds of Malacca Leaf (Phyllanthus emblica) after Triple Extraction with N-Hexane, Ethyl Acetate, and Ethanol’, The Scientific World Journal, 2020, p. 2739056. doi: 10.1155/2020/2739056.
Balangcod, T. D. et al. (2012) ‘Phytochemical screening and Antibacterial activity of selected medicinal plants of Bayabas , Sablan , Benguet Province , Cordillera Administrative Region , Luzon , Philippines’, Indian Journal of Traditional Knowledge, 11(4), pp. 580–585.
Balasubramanian, D. et al. (2017) ‘Staphylococcus aureus pathogenesis in diverse host environments’, Pathogens and Disease, 75(1), pp. 1–13. doi: 10.1093/femspd/ftx005.
Belkum, A. Van and Schrenzel, J. (2014) ‘Infection , Genetics and Evolution Staphylococcus aureus?: The innocent culprit??’, Infection, Genetics and Evolution, 21(September 2013), p. 509. doi: 10.1016/j.meegid.2013.09.001.
Van Belkum, A. and Schrenzel, J. (2014) ‘Staphylococcus aureus: The innocent culprit?’, Infection, Genetics and Evolution, 21(September 2013), p. 509. doi: 10.1016/j.meegid.2013.09.001.
De Boer, H. J. and Cotingting, C. (2014) ‘Medicinal plants for women’s healthcare in southeast Asia: A meta-analysis of their traditional use, chemical constituents, and pharmacology’, Journal of Ethnopharmacology, 151(2), pp. 747–767. doi: 10.1016/j.jep.2013.11.030.
BPS Aceh Selatan (2016) Aceh Selatan dalam Data. Tapak tuan: BPS Aceh Selatan.
Ernilasari, E. et al. (2021) ‘Antibacterial activity of leaves, flowers, and fruits extract of Etlingera elatior from Nagan Raya District, Indonesia against Escherichia coli and Staphylococcus aureus’, Biodiversitas Journal of Biological Diversity, 22(10).
Fan, Z. et al. (2015) ‘Blumea balsamifera Oil for the Acceleration of Healing of Burn Injuries’, Molecules, 1, pp. 17166–17179. doi: 10.3390/molecules200917166.
Gito, G. and Rochmawati, E. (2018) ‘Effectiveness of Modern Wound Dressing on the Growth of Staphylococcus Aureus Bacteria’, Jurnal Keperawatan, 9(2), p. 88. doi: 10.22219/jk.v9i2.5160.
Gonzalez, A. C. D. O. et al. (2016) ‘Wound healing - A literature review’, Anais Brasileiros de Dermatologia, 91(5), pp. 614–620. doi: 10.1590/abd1806-4841.20164741.
Huang YL, ZG, Z. and YX, W. (2016) ‘Komponen flavanoid non volatile blumea balsamifera’, (April), pp. 20–21.
Indah Imamah (2017) ‘Pengaruh Hidrogel Centalla Asiatica untuk Penyembuhan Luka Insisi’, Jurnal Ilmiah sehat bebaya, 1(2), pp. 125–131.
Isnawati A, Mariana Raini, S. A. (2016) ‘Standarisasi Simplisia dan Ekstrak Etanol Daun Sembung (Blumea balsamifera (L)) dari Tiga Tempat Tumbuh.pdf’, Media Litbang kesehatan, 16(2), pp. 1–6.
Katno, Sari Haryanti and Triyono, A. (2009) ‘Uji daya hambat ekstrak etanol daun sembung (Blumea balsamifera terhadap pertumbuhan mikroba E. coli, S. aereus dan C. albicans.’, Jurnal Tumbuhan Obat Indonesia, 2(1).
Kinho, J. and Diah I.D.A., Supratman T., Harwiyaddin K., Yermias K., Syamsir S., M. C. . (2011) Tumbuhan Obat Tradisional Di Sulawesi Utara Jilid I Julianus Kinho Diah Irawati Dwi Arini Supratman Tabba Harwiyaddin Kama Yermias Kafiar Syamsir Shabri. Available at: http://www.forda-mof.org/files/Tumbuhan Obat Tradisional di Sulut Jilid I.pdf.
Kubota, H. et al. (2009) ‘Anti-Obesity Effect of Blumea balsamifera Extract in 3T3-L1 Preadipocytes and Adipocytes’, The American Journal of Chinese Medicine, 37(5), pp. 843–854. doi: 10.1142/S0192415X09007326.
Lee, D. G. et al. (2012) ‘Analysis of apigenin in Blumea balsamifera Linn DC . and its inhibitory activity against aldose reductase in rat lens’, Journal of Agricultural Chemistry and Environment, 1(1), pp. 28–33. doi: 10.4236/jacen.2012.11005.
Mag, P. et al. (2014) ‘Phytochemical compositions of volatile oil from Blumea balsamifera and their biological activities’, 10(39). doi: 10.4103/0973-1296.137377.
Masyudi, Hanafiah, M., Usman, S., Rinidar, et al. (2022) ‘Phytochemical screening and GC-MS analysis of bioactive compounds of Blumea balsamifera leaf extracts from South Aceh , Indonesia’, Biodiversitas, 23(3). doi: 10.13057/biodiv/d2303xx.
Masyudi, Hanafiah, M., Usman, S. and Marlina (2022) ‘Phytochemical screening and GC-MS analysis of bioactive compounds of Blumea balsamifera leaf extracts from South Aceh , Indonesia’, Biodiversitas, 23(3), pp. 1346–1354. doi: 10.13057/biodiv/d230319.
Masyudi and Usman, S. (2019) ‘Creating Synergy between Village Midwives and Traditional Midwives?: To Reduce the Mortality Rate of Mothers and Babies?: In the Central Kluet Sub-District Health Centre in South Aceh Creating Synergy between Village Midwives and Traditional Midwives?: To’, Journal of Physics: Conference Series, 1232. doi: 10.1088/1742-6596/1232/1/012051.
Matuschek, E., Brown, D. F. J. and Kahlmeter, G. (2013) ‘Development of the EUCAST disk diffusion antimicrobial susceptibility testing method and its implementation in routine microbiology laboratories’, Clinical Microbiology and Infection, 20(4), pp. O255–O266. doi: 10.1111/1469-0691.12373.
Morales, G. et al. (2003) ‘Secondary metabolites from four medicinal plants from northern Chile: Antimicrobial activity and biotoxicity against Artemia salina’, Boletin de la Sociedad Chilena de Quimica, 48(2), pp. 13–18. doi: 10.4067/s0717-97072003000200002.
Nuryadin, M. (2017) ‘Formulasi dan uji penyembuhan luka gel sembung rambat’, Farmasi, Fakultas MIPA Universitas Sriwijaya.
Organization, W. H. (2013) WHO traditional medicine strategy: 2014-2023.
Pang, Y., Wang, D., Fan, Z., et al. (2014) ‘-A fitokimia dan Farmakologi Ulasan’, pp. 9453–9477.
Pang, Y., Wang, D., Hu, X., et al. (2014) ‘EXPERIMENTAL STUDY Effect of volatile oil from Blumea Balsamifera ( L .) DC . leaves on wound healing in mice’, Journal of Traditional Chinese Medicine, 34(6), pp. 716–724. doi: 10.1016/S0254-6272(15)30087-X.
Pang, Y. et al. (2017) ‘Effects and Mechanisms of Total Flavonoids from Blumea balsamifera ( L .) DC . on Skin Wound in Rats’, International journal of moleculer science, 18(12), p. 2766. doi: 10.3390/ijms18122766.
Sakee, U., Maneerat, S. and Cushnie, T. P. T. (2011) ‘Natural Product Research?: Formerly Natural Product Letters Antimicrobial activity of Blumea balsamifera ( Lin .) DC . extracts and essential oil’, Natural Product Research, (December 2014), pp. 37–41. doi: 10.1080/14786419.2010.485573.
Seriana, I. et al. (2021) ‘PHYTOCHEMICALS CHARACTERIZATIONS OF NEEM ( Azadirachta indica A . Juss ) LEAVES ETHANOLIC EXTRACT?: AN IMPORTANT MEDICINAL PLANT AS MALE’, 14(1), pp. 343–350. doi: 10.31788/ RJC.2021.1415899.
Singh, A., Kumar, A. and Srivastava, V. (2018) ‘Extraction of bioactive compound from different medicinal plants and rhizospheric soil bacteria and their comparative study against human pathogens’, Journal of Pharmacognosy and Phytochemistry, 7(6), pp. 528–533.
Soekiman, S. (2016) Infeksi Nosokomial Di Rumah Sakit - Hospital Nososcomial Infections. Pertama. Edited by Mariyam. Surabaya.
Tong, S. Y. C. et al. (2015) ‘Staphylococcus aureus infections: Epidemiology, pathophysiology, clinical manifestations, and management’, Clinical Microbiology Reviews, 28(3), pp. 603–661. doi: 10.1128/CMR.00134-14.
Viena, V. et al. (2018) ‘Biodiversitas tumbuhan obat oleh masyarakat gampong pulo seunong kecamatan tangse kabupaten pidie’, Elkawnie: Journal of Islamic Science and Technology, 4(1), pp. 89–100. doi: 10.22373/ekw.v4i1.3027.
Walil, K. and Roslim, D. I. (2021) ‘Antibacterial activity of leaves , flowers , and fruits extract of Etlingera elatior from Nagan Raya District , Indonesia against Escherichia coli and Staphylococcus aureus’, Biodiversitas, 22(10), pp. 4457–4464. doi: 10.13057/biodiv/d221039.
Yan, X., Xiang, L. and Jian-wei, C. (2015) ‘Anti-inflammatory,Analgesic and Hemostasis Activities of Different Extracting Fractions of Blumea balsamifera Residue’, natural Product Research and Development, 27(6), pp. 1086–1091. doi: 10.16333/j.1001-6880.2015.06.027.
Yong-lin, H., Zhi-guo, Z. and Yong-xin, W. (2016) ‘Determination of total flavonoid in different sections of Blumea balsanifera’, CNKI.
Yuan, H. et al. (2016) ‘The Traditional Medicine and Modern Medicine from Natural Products’, Molecules, 21, p. 559.