Morpho-anatomical characterization and DNA barcode of Cosmos caudatus Kunth.

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HERY PURNOBASUKI
https://orcid.org/0000-0002-0562-2058
GALUH AYU RAKHASHIWI
JUNAIRIAH
https://orcid.org/0000-0003-0151-222X
DWI KUSUMA WAHYUNI
https://orcid.org/0000-0002-1149-1103
RAMADHANI EKA PUTRA
https://orcid.org/0000-0003-2261-9405
RIKA RAFFIUDIN
https://orcid.org/0000-0002-5373-9445
RC HIDAYAT SOESSILOHADI
https://orcid.org/0000-0002-2614-3785

Abstract

Abstract. Purnobasuki H, Rakhashiwi GA, Junairiah, Wahyuni DK, Putra RE, Raffiudin R, Soessilohadi RCH. 2022. Morpho-anatomical characterization and DNA barcode of Cosmos caudatus Kunth. Biodiversitas 23: 4097-4108. Secondary metabolites in plants have various benefits, therefore their distribution in plant body parts is important to study. The identification process of plants should be conducted using several characters, including morphological and molecular data. This study aimed to identify and confirm Cosmos caudatus Kunth using morpho-anatomy characters and DNA barcodes. The plants used were three samples taken from the Husada Graha Famili Park, Surabaya. The morpho-anatomical investigation was carried out descriptively, while the DNA barcoding study was processed by amplifying and sequencing the rbcL and matK genes. Morphological studies indicate that the roots and stems of C. caudatus are similar to the organs of dicots shrubs in general. The leaves have a pinnate type with two different colors on each side of the leaf. Compound flowers are cup-type with two types of flowers, namely disc flowers and ray flowers. Based on anatomical observation, all vegetative organs have the same tissue as dicotyledonous plants. The anatomical characteristic of C. caudatus lies in its trichomes due to its anthocyanin content and stomata which are anomocytic and anisocytic types. DNA barcoding results showed that the three sample plants had identical similarities with several Cosmos genera contained in the GenBank database with percentage identities values ??above 98%, query covers 96-100%, and e-values ??of 0.

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References
REFERENCES
Ajaykumar, T. V., Anandarajagopal, K., Sunilson, J. A. J., Arshad, A., Jainaf, R. A. M., & Venkateshan, N. 2012. Anti-inflammatory activity of Cosmos caudatus. Int J Univers Pharm Bio Sci, 1(2), 40-8.
Alvarez I, Wendel JF. 2003. Ribosomal ITS sequences and plant phylogenetic inference. Mol Phylogenet Evol 29: 417-434
Anonim, 2008, Deskripsi Morfologi Kenikir dalam (https://ccrc.farmasi.ugm.ac.id/?page_id=101), diakses 13 September 2020
Bisht, V.K and Purohit, V., 2010, Medicine and Aromatic Plants Diversity of Asteraceae in Uttarakhand, Herbal Research & Development Institute. Gopeshwar, Uttarakhand, India
CBOL (Consortium for the Barcode of Life) Plant Working Group, 2009, A DNA Barcode for Land Plant. Proceeding of the National Academy of Sciences, 106: 12794-12797
Chen, S., Pang, X., Song, J., Shi, L., Yao, H., Han, J., Leon, C., 2014, A renaissance in herbal medicine identification: from morphology to DNA, Biotech Adv, 32: 1237–1244.
De Boer, H.J., Ichim, M.C., Newmaster, S.G., 2015, DNA barcoding and pharmacovigilance of herbal medicines, Drug Saf, 38: 611–620
Dharmayanti, I., 2011, Filogenetika molekuler: Metode taksonomi organisme berdasarkan sejarah evolusi, WARTAZOA, 21(1): 1-10
Djumhana, N., Wuryastuti, S., Hendrawati, Y. dan Peristiwati, 2006, Konsep Dasar Biologi, UPI Press, Bandung
Fazekas, A.J. Burgess, K.S. Kesanakurti, P.R., Graham, S.W., Newmaster, S.G., Husband, B.C., Hajibabaei, M. and Barrett, S.C.H., 2008, Multiple Multilocus DNA Barcodes from The Plastid Genome Discriminate Plant Species Equally Well, PlosOne, 3(7): e2802
Imaniar, R., Latifah, dan Sugiyo, W., 2013, Ekstraksi dan Karakteristik Senyawa Bioaktif dalam Kenikir Sebagai Bahan Bioinsektisida Alami, Indo. J. Chem. Sci, 2(1) : 51-55
Irmawati, 2003, Perubahan Keragaman Genetik Ikan Kerapu Tikus Generasi Pertama Pada Stok Hatchery, Thesis Institut Pertanian Bogor
Izza, N., Dewi, S.R., Putranto, A.W., Yuneri, D.R., Dachi, M.Y.S., 2016, Ekstraksi senyawa fenol daun kenikir (Cosmos caudatus Kunth.) dengan pulse electric field (PEF), J Teknol Pertani, 17 (2): 91-96. (in Indonesian)
Kress WJ, Wurdack KJ, Zimmer EA, Weigt LA, Janzen DH (2005) Use of DNA barcodes to identify flowering plants. Proceedings of the
National Academy of Sciences of the United States of America. 2005 May 31. 102: 8369-8374.
Kress WJ and Erickson DL. 2007 A two-locus global DNA barcode for land plants: The coding rbcL gene complements the non-coding trnH-psbA spacer region. PLoS ONE 2:e508.
Kumolo, Fredian, B., Utami, S., 2011, Jenis-jenis Tumbuhan Anggota Famili Asteraceae di Wana Wisata Nglimut Gonoharjo Kabupaten Kendal Jawa Tengah. Bioma, 13(1): 1410-8801. (in Indonesian)
Lahaye, R., Michelle van der Bank., Bogarin, D., Warner, J., Pupulin, F., Gigot, G., Maurin, O., Duthoit, S., Timothy G., Barraclough, AND Savolainen, V., 2008, DNA barcoding the floras of biodiversity hotspots, PNAS, 105(8): 2923-2928
Loh, S.P., Nadira, O., 2011, In vitro inhibitory potential of selected Malaysian plants against key enzymes involved In hyperglycemia and hypertension, Malays J Nutr, 17(1): 77-86
Manurung, J., Prakasa, H., Tanjung, U.J., dan Harsono, T., 2018, Hubungan kekerabatan spesies dalam genus Zanthoxylum menggunakan sekuen gen maturase K (matK) DNA Kloroplas, Jurnal Biosains, 4(2): 69-77.
Moshawih, S., Cheema, M. S., Ahmad, Z., Zakaria, Z .A. dan Hakim, M.N., 2017, A Comprehensive Review on Cosmos caudatus (Ulam Raja): Pharmacology, Ethnopharmacology, and Phytochemistry, International Research Journal of Education and Sciences, 1(1): 2550 – 2158
Newmaster, S. G., Fazekas, A. J. & Ragupathy, S. 2006. DNA barcoding in land Plants: evaluation of rbcL in a multigene tiered approach. Can. J. Bot. 84, 335–341.
Pebriana, R.B., Wardhani, B.W.K., Widayanti, E., Wijayanti, L.S., Wijayanti, T.R., Riyanto, S., Meiyanto, E., 2008, Pengaruh ekstrak metanolik daun kenikir (Cosmos caudatus Kunth.) terhadap pemacuan apoptosis sel kanker payudara, Pharmacon, 9 (1): 21-26. (in Indonesian)
Rasdi, N.H.M., Samah, O., Sule, A., Ahmed, Q.U., 2010, Antimicrobial studies of Cosmos caudatus Kunth (Compositae), J Med Plants, 4(8) : 669-673
Safita, G., Endah R., dan Livia, S, 2015, Uji Aktivitas Antibakteri Daun Kenikir (Cosmos caudatus Kunth.) dan Daun Sintrong (Crassocephalum crepidioides (Benth.) S. Moore) terhadap Bakteri Staphylococcus aereus dan Pseudomonas aeruginosa, Prosiding Penelitian Sivitas Akademika Unisba (Kesehatan dan Farmasi), Bandung, 421- 428. (in Indonesian)
Sambrook, J. dan Russel D.W., 1989, Molecular Cloning: A Laboratory Manual, Cold-Spring Harbor Laboratory Press, New York
Sass C, Little DP, Stevenson DW, Specht CD. 2007. DNA barcoding in the Cycadales: testing the potential of proposed barcoding markers for
species identification of cycads. PLoS ONE 2: e1154. DOI: 10.1371/journal.pone.0001154.
Stoeckle, M. and Ausubel, J. H., 2003, Barcode of Life, The Rockfeller University, New York
Sutikno, 2014, Petunjuk Praktikum Mikroteknik Tumbuhan, Universitas Gadjah Mada, Yogyakarta, 29-32 (in Indonesian)
Wahyuni, D.K., Rahayu, S., Purnama, P.R., Saputro, T.B., Suharyanto, Wijayanti, N., Purnobasuki, H., 2019, Morpho-anatomical structure and DNA barcode of Sonchus arvensis L, Biodiversitas, 20: 2417-2426
Yusoff, NAH., Bustamam, MS., Abas, F., Khatib, A. and Rukayadi, Y. 2014. Antimicrobial activity of Cosmos caudatus extract against foodborne pathogens. Journal of Pure AND Applied Microbiology 8(5): 3681-3688.
Yustinadewi, Yustiantara, P. S. and Narayani, Inna. 2018. Teknik Perancangan Primer untuk Sekuen Gen Mdr-1 Varian 1199 Pada Sampel Buffy Coat Pasien Anak Dengan LLA. Jurnal Metamorfosa. 5(1): 105-111. (in Indonesian)

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