Evaluation of non-timber forest products used as medicinal plants from East Kalimantan (Indonesia) to inhibit ?-glucosidase and free radicals
##plugins.themes.bootstrap3.article.sidebar##
Issue
Section
Articles
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
##plugins.themes.bootstrap3.article.main##
Abstract
Abstract. Ramadhan R, Tosepu R, Phuwapraisirisan P, Amirta R, Phontree K, Firdaus YFH, Abdulgani N, Muttaqin MZ, Saparwardi. 2022. Evaluation of non-timber forest products used as medicinal plants from East Kalimantan (Indonesia) to inhibit ?-glucosidase and free radicals. Biodiversitas 23: 5551-5558. Non-timber forest products (NTFPs) have essential uses as medicines, food sources, and traditional ceremonies for the local people living in forest areas. Medicinal plants are one of the non-timber forest products used as traditional medicine by local people in East Kalimantan. This study was conducted to search for new natural antidiabetic and antioxidant resources from medicinal plants used traditionally in Mahakam Ulu District, East Kalimantan, based on ethnopharmacological information. The present study evaluates non-timber forest products as in vitro antidiabetic (rat intestinal ?-glucosidase inhibitory activity) and antioxidant (free radical scavenging activity against DPPH, ABTS, and Nitric Oxide) activities. The antidiabetic and antioxidant activities were evaluated using ?-glucosidase inhibition assay and free radical scavenging methods. This study demonstrated that methanol extracts of Flacourtia rukam Zoll. & Moritzi, Syzygium chloranthum (Duthie) Merr. & L.M. Perry and Shorea balangeran Burck exhibited strong ?-glucosidase inhibitory activity with maltose as a substrate with IC50 values of 0.034 mg/mL, 0.039 mg/mL, and 0.039 mg/mL, respectively. On the other hand, methanol extract of S. chloranthum displayed the strongest ?-glucosidase inhibitory activity in sucrose as a substrate, similar to the IC50 value of the quercetin as a positive control. Furthermore, the antioxidant test showed that all medicinal plant extracts from East Kalimantan are good sources of natural antioxidants indicated by their IC50 values. The results of this study support the scientific background of the uses of medicinal plant extracts from East Kalimantan as folk medicine.
##plugins.themes.bootstrap3.article.details##
References
Abuga I, Sulaiman SF, Wahab RA, Ooi KL, Rasad MBSA. 2022. Phytochemical constituens and antibacterial activities of 45 Malay traditional medicinal plants. J Herb Med 32: 100496. DOI: 10.1016/j.hermed.2021.100496
Adouni K, Bendif H, Zouaoui O, Kraujalis P, Flamini G, Venskutonis PR, Achour L. 2022. Antioxidant activity of extracts obtained by high-pressure extraction procedures from Asparagus stipularis Forssk. S Afr J Bot 146: 789-793. DOI: 10.1016/j.sajb.2021.12.027
Afifi NI, Moawad AS, Zaki MA, Rateb ME, Rashed MH, Saleh IG, Hetta MH, Mohammed RM. 2021. Four new phenolic and antiparasitic secondary metabolites from Flacourtia rukam Zoll. & Mortizi. Nat Prod Res 2021. DOI: 10.1080/14786419.2021.1875462
Akyuz M, Yabo-Dambagi L, Kilic T, Cakir A. 2022. Antidiabetic, neuroprotective and antioxidant potentials of different parts of Pistacia terebinthus fruits. S Afr J Bot 147: 443-456. DOI: 10.1016/j.sajb.2022.01.040.
Arroussi J, Ouerfelli M, Smaoui A, Ahmed HB, Kaâb LBB. 2022. Antioxidant activity of seven plant extracts collected from Tunisia and their allelopathic potential on Lactuca sativa L. and Phalaris minor L. S Afr J Bot 148: 135-143. DOI: 10.1016/j.sajb.2022.04.029.
Arung ET, Kuspradini H, Kusuma IW, Shimizu K, Kondo R. 2012. Validation of Eupatorium triplinerve Vahl leaves, a skin care herb from East Kalimantan, using a melanin biosynthesis assay. J Acupunct Meridian Stud 5: 87-92. DOI: 10.1016/j.jams.2012.01.003
Binh TD, Staerk D, Jäger AK. 2016. Screening for potential ?-glucosidase and ?-amylase inhibitory constituents from selected Vietnamese plants used to treat type 2 diabetes. J Ethnopharmacol 186: 189-195. DOI: 10.1016/j.jep.2016.03.060
Bourjot M, Leyssen P, Eydoux C, Guillemot JC, Canard B, Rasoanaivo P, Guéritte F, Litaudon M. 2012. Flacourtosides A-F, phenolic glycosides isolated from Flacourtia ramontchi. J Nat Prod 15: 752-758. DOI: 10.1021/np300059n
Habu JB, Ibeh BO. 2015. In vitro antioxidant capacity and free radical scavenging evaluation of active metabolite constituents of Newbouldia laevis ethanolic leaf extract. Biol Res 48: 1-10. DOI: 10.1186/s40659-015-0007-x
Hu YK, Wang L, Wang JH, Li MJ, Li F, Yang J, Zhao Y. Resorcinol derivatives with ?-glucosidase inhibitory activities from Syzygium samarangense. Nat Prod Res 35: 5948-5953. DOI: 10.1080/14786419.2020.1805606
Indrianingsih AW, Tachibana S, Itoh K. 2015. In Vitro evaluation of antioxidant and ?-glucosidase inhibitory assay of several tropical and subtropical plants. Procedia Environ Sci 28: 639-648. DOI: 10.1016/j.proenv.2015.07.075
Ji Y, Liu D, Jin Y, Zhao J, Zhao J, Li H, Li L, Zhang H, Wang H. 2021. In vitro and in vivo inhibitory effect of anthocyanin-rich bilberry extract on ?-glucosidase and ?-amylase. LWT 145: 111484. DOI: 10.1016/j.lwt.2021.111484
Kanwugu ON, Glukhareva TV, Danilova IG, Kovaleva EG. Natural antioxidant in diabetes treatment and management: prospect of astaxantin. Crit Rev Food Sci Nutr 2021. DOI: 10.1080/10408398.2021.1881434
Khan I, Ullah Z, Shad AA, Fahim M, Öztürk M. In vitro antioxidant, anticholinesterase inhibitory, and antimicrobial activity studies of Terminalia chebula (Retz) and Terminalia arjuna (Roxb). S Afr J Bot 146: 395-400. DOI: 10.1016/j.sajb.2021.11.016
Khongkarat P, Ramadhan R, Phuwapraisirisan P, Chanchao C. 2020. Safflospermidines from the bee pollen of Helianthus annuus L. exhibit a higher in vitro antityrosinase activity than kojic acid. Heliyon 6: e03638. DOI: 10.1016/j.heliyon.2020.e03638
Kusuma IW, Murdiyanto, Arung ET, Syafrizal, Kim Y. 2014. Antimicrobial and antioxidant properties of medicinal plants used by the Bentian tribe from Indonesia. Food Sci Hum Wellness 3: 191-196. DOI: 10.1016/j.fshw.2014.12.004
Li YY, Hu YK, Wang JH, Huang YP, Zeng GJ, Yang Y, Zhao Y. ?-Glucosidase inhibitors from Syzygium latilimbum. Chem Nat Compd 57: 551-552. DOI: 10.1007/s10600-021-03412-z
Marjoni MR and Zulfisa A. 2017. Antioxidant activity of methanol extract/fractions of Senggani leaves (Melastoma candidum D. Don). Pharm Anal Acta 8: 1-6. DOI: 10.4172/2153-2435.1000557
Mbopi PY, Fozeng HDS, Nguekeu YMM, Bitchagno GTM, Ngantchouko CBN, Awouafack MD, Opatz T, Ngouela SA, Morita H, Tene M. 2021. Chemical constituents, total phenolic content, antioxidant activity and bactericidal effect of Dicliptera verticillate (Acanthaceae). S Afr J Bot 142: 216-221. DOI: 10.1016/j.sajb.2021.07.001.
Mipun P, Bhat NA, Borah D, Kumar Y. Non-timber forest products and their contribution to healthcare and livelihood security among the Karbi tribe in Northeast India. Ecol Process 8: 1-21. DOI: 10.1186/s13717-019-0194-4
Proen?a C, Freitas M, Ribeiro D, Oliveira EFT, Sousa JLC, Tome SM, Ramos MJ, Silva AMS, Fernandes PA, Fernandes E. 2017. ?-Glucosidase inhibition by flavonoids: An in vitro and in silico structure-activity relationship study. J Enzyme Inhib Med Chem 32: 1216-1228. DOI: 10.1080/14756366.2017.1368503.
Pyner A, Nyambe-Silavwe H, Williamson G. 2017. Inhibition of human and rat sucrase and maltase activities to assess antiglycemic potential: Optimization of the assay using acarbose and polyphenols. J Agric Food Chem 65: 8643-8651. DOI: 10.1021/acs.jafc.7b03678
Ramadhan R, Kristanti AN, Amirta R, Kusuma IW, Phuwapraisirisan P, Haqiqi MT, Saparwadi. 2019. Screening for potential antidiabetes and antioxidant activities of selected plants from East Kalimantan, Indonesia. Biodiversitas 20: 1820-1826. DOI: 10.13057/biodiv/d200705
Ramadhan R, Phuwapraisirisan P, Kusuma IW, Amirta R. 2020. Ethnopharmacological evaluation of selected East Kalimantan flora for diabetes therapy: The isolation of lupane triterpenoids as ?-glucosidase inhibitors from Ceriop tagal (Perr). C.B. Robb. Rasayan J Chem 13: 1727-1734. DOI: 10.31788/RJC.2020.1335559
Ratnadewi AAI, Wahyudi LD, Rochman J, Susilowati, Nugraha AS, Siswoyo TA. 2020. Revealing anti-diabetic potency of medicinal plants of Meru Betiri Nasional Park, Jember – Indonesia. Arab J Chem 13: 1831-1836. DOI: 10.1016/j.arabjc.2018.01.017
Riyanti S, Dewi PS, Windyaswari AS, Azizah SAN. 2020. Alpha-glucosidase inhibitory activities of bungur (Lagerstroemia loudonii Teijsm. & Binn.) leaves and fruits. IOP Conf Ser Earth Environ Sci 462: 012042. DOI: 10.1088/1755-1315/462/1/012042
Seo KH, Ra JE, Lee SJ, Lee JH, Kim SR, Lee JH, Seo WD. 2015. Anti-hyperglycemic activity of polyphenols isolated from barnyard millet (Echinochloa utilis L) and their role inhibiting ?-glucosidase. J Korean Soc Appl Biol Chem 58: 571–579. DOI: 10.1007/s13765-015-0070-6
Seyrekoglu F, Temiz H, Eser F, Yildirim C. 2022. Comparison of antioxidant activities and major constituents of three Hypericum species (H. perforatum, H. scabrum and H. origanifolium) from Turkey. S Afr J Bot 146: 723-727. DOI: 10.1016/j.sajb.2021.12.012
Shackleton CM, Ticktin T, Cunningham AB. 2018. Nontimber forest products as ecological and biocultural keystone species. Ecol 23: 22. DOI: 10.5751/ES-10469-230422
Sashidhara KV, Singh SP, Varshney S, Beg M, Gaikwad AN. 2014. Poliothrysoside and its derivatives as novel insulin sensitizers potentially driving AMPK activation and inhibiting adipogenesis. Eur J Med Chem 86: 570-577. DOI: 10.1016/j.ejmech.2014.09.015
Subramanian R, Subbramaniyan P, Raj V. Antioxidant activity of the stem bark of Shorea roxburghii and its silver reducing power. Springerplus 2: 1-11. DOI: 10.1186/2193-1801-2-28
Subramanian R, Raj V, Manigandan K, Elangovan. 2015. Antioxidant activity of hopeaphenol isolated from Shorea roxburghii stem bark extract. J Taibah Univ Sci 9: 237-244. DOI: 10.1016/j.jtusci.2014.11.004
Sukara E. 2014. Tropical forest biodiversity to provide food, health and energy solution of the rapid growth of modern society. Procedia Environ Sci 20: 803-808. DOI: 10.1016/j.proenv.2014.03.097
Thai CT, Duong TH, Trung NT, Phan DC, Nguyen QV, Nguyen TL, Wanatabe K, Do TT, Huynh TN, Tran H. 2020. Rukamtenol, a new spiro compound isolated from Flacourtia rukam Zoll. & Mortizi growing in Vietnam. Nat Prod Res 36: 1966-1972. DOI:10.1080/14786419.2020.1839451
Xiao F, Xu T, Lu B, Liu R. 2020. Guidelines for antioxidant assays for food components. Food Frontiers 1: 60-69. DOI: 10.1002/fft2.10.
Venkatachalam U, Muthukrishnan S. 2012. Free radical scavenging activity of ethanolic extract of Desmodium gangeeticum. J Acute Med 2: 36-42. DOI: 10.1016/j.jacme.2012.04.002
Most read articles by the same author(s)
- SYAFRIZAL, RICO RAMADHAN , IRAWAN WIJAYA KUSUMA, SAAT EGRA, KUNIYOSHI SHIMIZU, MAMORU KANZAKI, ENOS TANGKE ARUNG, Diversity and honey properties of stingless bees from meliponiculture in East and North Kalimantan, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 21 No. 10 (2020)
- ELISA HERAWATI, RICO RAMADHAN, FARIDA ARIYANI, MARJENAH, IRAWAN WIJAYA KUSUMA, WIWIN SUWINARTI, DJUMALI MARDJI, RUDIANTO AMIRTA, ENOS TANGKE ARUNG, Phytochemical screening and antioxidant activity of wild mushrooms growing in tropical regions , Biodiversitas Journal of Biological Diversity: Vol. 22 No. 11 (2021)
- RUDIANTO AMIRTA, AHMAD MUKHDLOR, DEWI MUJIASIH, ELIS SEPTIA, SUPRIADI SUPRIADI, DWI SUSANTO, Suitability and availability analysis of tropical forest wood species for ethanol production: a case study in East Kalimantan , Biodiversitas Journal of Biological Diversity: Vol. 17 No. 2 (2016)
- DEWI HIDAYATI, ANNISAA RIZQI NURINDRA, NURLITA ABDULGANI, EDWIN SETIAWAN, NOVA MAULIDINA, NUR SYAHRONI, YEYES MULYADI , Fish disease study of Asian seabass (Lates calcarifer) in a floating marine cage: Endoparasite and blood profile , Biodiversitas Journal of Biological Diversity: Vol. 22 No. 10 (2021)
- RICO RAMADHAN, ALFINDA NOVI KRISTANTI, RUDIANTO AMIRTA, IRAWAN WIJAYA KUSUMA, PREECHA PHUWAPRAISIRISAN, MUHAMMAD TAUFIQ HAQIQI, SAPARWADI SAPARWADI, Screening for potential antidiabetes and antioxidant activities of selected plants from East Kalimantan, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 20 No. 7 (2019)
- RUDIANTO AMIRTA, SYAFFIYA ISNU NAFITRI, RETNO WULANDARI, YULIANSYAH YULIANSYAH, WIWIN SUWINARTI, KRISHNA PURNAWAN CANDRA, TAKASHI WATANABE, Comparative characterization of Macaranga species collected from secondary forests in East Kalimantan for biorefinery of unutilized fast growing wood , Biodiversitas Journal of Biological Diversity: Vol. 17 No. 1 (2016)
- RICO RAMADHAN, PREECHA PHUWAPRAISIRISAN, RUDIANTO AMIRTA, MUHAMMAD FADIL BAGAS DARMAWAN, KAUTSAR UL-HAQ, IRAWAN WIJAYA KUSUMA, HERY SUWITO, NURLITA ABDULGANI, AHMAD MUKHDLOR, SAPARWADI, The potency of selected ethnomedicinal plants from East Kalimantan, Indonesia as antidiabetic agents and free-radical scavengers , Biodiversitas Journal of Biological Diversity: Vol. 23 No. 4 (2022)
- YULIANSYAH, MUHAMMAD TAUFIQ HAQIQI, ELIS SEPTIA, DEWI MUJIASIH, HELMI ALFATH SEPTIANA, KRISNA ADIB SETIAWAN, BUDHI SETIYONO, EDDY MANGOPO ANGI, SAPARWADI, NUR MAULIDA SARI, IRAWAN WIJAYA KUSUMA, RUJEHAN, WIWIN SUWINARTI, RUDIANTO AMIRTA, Short Communication: Diversity of plant species growing during fallow period of shifting cultivation and potential of its biomass for sustainable energy production in Mahakam Ulu, East Kalimantan, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 20 No. 8 (2019)