The diversity and susceptibility against antibiotics of Salmonella spp. clinical isolates from Yogyakarta, Indonesia
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Abstract. Kurniati E, Retnaningrum E, Wijayanti N, Wibawa T. 2022. The diversity and susceptibility against antibiotics of Salmonella spp. clinical isolates from Yogyakarta, Indonesia. Biodiversitas 23: 5806-5813. Salmonella spp. are bacteria that causes enteric fever. Salmonella infection is a serious burden in both developed and developing countries, including Indonesia. Because of infrastructure limitation, Salmonella spp. infection treatment mostly based on clinical observation without proper identification of the causative bacteria. Although the clinical manifestation of the diseases is difficult to distinguished, it is believed that Salmonella spp. diversity is high in the community. This work aims to analyze the diversity of Salmonella spp. and their susceptibility against antibiotics. The Salmonella spp clinical isolates obtained from three clinical laboratories were subjected to cultivation, phenotypic characterization, and further biochemical and molecular characterization. Biochemical properties of strains were analyzed, whereas protein profiles were analyzed using the SDS-PAGE method. The molecular characterization was carried out by sequencing of 16S rRNA encoded gene. Numeric-phenetic clustering analysis of the 18 Salmonella spp clinical isolates showed that, they were allocated to two large clusters, namely Cluster I and Cluster II. Reconstruction of the phylogeny tree of the 18 clinical isolates and the reference strains showed that all of them belonged to one clade, namely the Salmonella enterica subsp. Enterica. However, it was recognized also six sub-clades. The antibiotic susceptibility pattern of Salmonella spp. revealed that practically all antibiotics (80%) had a high proportion of susceptibility, except for cefazoline, gentamicin, and amikacin, which have zero susceptibility. Numeric-phenetic clustering analysis combined with phylogeny tree reconstruction showed the diversity of Salmonella spp. circulating in Yogyakarta with alarming signal of certain antibiotic resistance.
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References
Aksakal, A. (2010). Analysis of whole cell protein profiles of Salmonella serovars isolated from chicken, turkey and sheep faeces by SDS-PAGE. Veterinární Medicína, 55(No. 6), 259–263. https://doi.org/10.17221/2986-VETMED
Arora, P., Thorlund, K., Brenner, D. R., & Andrews, J. R. (2019). Comparative accuracy of typhoid diagnostic tools: A Bayesian latent-class network analysis. PLOS Neglected Tropical Diseases, 13(5), e0007303. https://doi.org/10.1371/journal.pntd.0007303
Beig, F. K., Ahmad, F., Ekram, M., & Shukla, I. (2010). Typhidot M and Diazo test vis-à-vis blood culture and Widal test in the early diagnosis of typhoid fever in children in a resource poor setting. Brazilian Journal of Infectious Diseases, 14(6), 589–593. https://doi.org/10.1590/S1413-86702010000600007
Darmawati, S., Sembiring, L., Asmara, W., & Artama, W. T. (2011). Klasifikasi Numerik-fenetik Salmonella typhi Asal Jawa Tengah dan Daerah Istimewa Yogyakarta Berdasarkan Hasil Karakterisasi Fenotipik. Biota?: Jurnal Ilmiah Ilmu-Ilmu Hayati, 128–132. https://doi.org/10.24002/biota.v16i1.67
de Carli, S., Gräf, T., Kipper, D., Lehmann, F. K. M., Zanetti, N., Siqueira, F. M., Cibulski, S., Fonseca, A. S. K., Ikuta, N., & Lunge, V. R. (2018). Corrigendum to “Molecular and phylogenetic analyses of Salmonella Gallinarum trace the origin and diversification of recent outbreaks of fowl typhoid in poultry farms” [Vet. Microbiol. 212 (2017) 80–86]. Veterinary Microbiology, 213, 150. https://doi.org/10.1016/j.vetmic.2017.12.001
de Kraker, M. E. A., Stewardson, A. J., & Harbarth, S. (2016). Will 10 Million People Die a Year due to Antimicrobial Resistance by 2050? PLOS Medicine, 13(11), e1002184. https://doi.org/10.1371/journal.pmed.1002184
Elkenany, R., Elsayed, M. M., Zakaria, A. I., El-sayed, S. A.-E.-S., & Rizk, M. A. (2019). Antimicrobial resistance profiles and virulence genotyping of Salmonella enterica serovars recovered from broiler chickens and chicken carcasses in Egypt. BMC Veterinary Research, 15(1), 124. https://doi.org/10.1186/s12917-019-1867-z
Eng, S.-K., Pusparajah, P., Ab Mutalib, N.-S., Ser, H.-L., Chan, K.-G., & Lee, L.-H. (2015). Salmonella?: A review on pathogenesis, epidemiology and antibiotic resistance. Frontiers in Life Science, 8(3), 284–293. https://doi.org/10.1080/21553769.2015.1051243
Gasem, M. H., Kosasih, H., Tjitra, E., Alisjahbana, B., Karyana, M., Lokida, D., Neal, A., Liang, C. J., Aman, A. T., Arif, M., Sudarmono, P., Suharto, Merati, T. P., Lisdawati, V., Siswanto, Siddiqui, S., & Lane, H. C. (2020). An observational prospective cohort study of the epidemiology of hospitalized patients with acute febrile illness in Indonesia. PLOS Neglected Tropical Diseases, 14(1), e0007927. https://doi.org/10.1371/journal.pntd.0007927
Gong, B., Li, H., Feng, Y., Zeng, S., Zhuo, Z., Luo, J., Chen, X., & Li, X. (2022). Prevalence, Serotype Distribution and Antimicrobial Resistance of Non-Typhoidal Salmonella in Hospitalized Patients in Conghua District of Guangzhou, China. Frontiers in Cellular and Infection Microbiology, 12(February), 1–7. https://doi.org/10.3389/fcimb.2022.805384
Gould, S. J. (1974). The First Decade of Numerical Taxonomy: Numerical Taxonomy . The Principles and Practice of Numerical Classification. PETER H. A. Sneath and Robert R. Sokal. Freeman, San Francisco, 1973. xvi, 574 pp., illus. $19.50. A Series of Books in Biology. Science, 183(4126), 739–740. https://doi.org/10.1126/science.183.4126.739
Gunn, J. S., Marshall, J. M., Baker, S., Dongol, S., Charles, R. C., & Ryan, E. T. (2014). Salmonella chronic carriage: epidemiology, diagnosis, and gallbladder persistence. Trends in Microbiology, 22(11), 648–655. https://doi.org/10.1016/j.tim.2014.06.007
Hatta, M., & Ratnawati, N. (2008). Enteric fever in endemic areas of Indonesia: an increasing problem of resistance. The Journal of Infection in Developing Countries, 2(04). https://doi.org/10.3855/jidc.222
Heithoff, D. M., Shimp, W. R., Lau, P. W., Badie, G., Enioutina, E. Y., Daynes, R. A., Byrne, B. A., House, J. K., & Mahan, M. J. (2008). Human Salmonella Clinical Isolates Distinct from Those of Animal Origin. Applied and Environmental Microbiology, 74(6), 1757–1766. https://doi.org/10.1128/AEM.02740-07
Jajere, S. M. (2019). A review of Salmonella enterica with particular focus on the pathogenicity and virulence factors, host specificity and antimicrobial resistance including multidrug resistance. Veterinary World, 12(4), 504–521. https://doi.org/10.14202/vetworld.2019.504-521
Jiang, M., Zhu, F., Yang, C., Deng, Y., Kwan, P. S. L., Li, Y., Lin, Y., Qiu, Y., Shi, X., Chen, H., Cui, Y., & Hu, Q. (2020). Whole-Genome Analysis of Salmonella enterica Serovar Enteritidis Isolates in Outbreak Linked to Online Food Delivery, Shenzhen, China, 2018. Emerging Infectious Diseases, 26(4), 789–792. https://doi.org/10.3201/eid2604.191446
Kovach. (2007). Package Statistical Variate Multi-Version 3.1 Users’ Manual Kovach Computing Services.
Lou, L., Zhang, P., Piao, R., & Wang, Y. (2019). Salmonella Pathogenicity Island 1 (SPI-1) and Its Complex Regulatory Network. Frontiers in Cellular and Infection Microbiology, 9(July), 1–12. https://doi.org/10.3389/fcimb.2019.00270
Nguyen Thi, H., Pham, T.-T.-T., Turchi, B., Fratini, F., Ebani, V. V., Cerri, D., & Bertelloni, F. (2020). Characterization of Salmonella spp. Isolates from Swine: Virulence and Antimicrobial Resistance. Animals, 10(12), 2418. https://doi.org/10.3390/ani10122418
Reed, T. A. N., Krang, S., Miliya, T., Townell, N., Letchford, J., Bun, S., Sar, B., Osbjer, K., Seng, S., Chou, M., By, Y., Vanchinsuren, L., Nov, V., Chau, D., Phe, T., de Lauzanne, A., Ly, S., & Turner, P. (2019). Antimicrobial resistance in Cambodia: a review. International Journal of Infectious Diseases, 85, 98–107. https://doi.org/10.1016/j.ijid.2019.05.036
Sela, I., Wolf, Y. I., & Koonin, E. v. (2016). Theory of prokaryotic genome evolution. Proceedings of the National Academy of Sciences, 113(41), 11399–11407. https://doi.org/10.1073/pnas.1614083113
Shaikh, S., Fatima, J., Shakil, S., Rizvi, S. Mohd. D., & Kamal, M. A. (2015). Antibiotic resistance and extended spectrum beta-lactamases: Types, epidemiology and treatment. Saudi Journal of Biological Sciences, 22(1), 90–101. https://doi.org/10.1016/j.sjbs.2014.08.002
Stanaway, J. D., Parisi, A., Sarkar, K., Blacker, B. F., Reiner, R. C., Hay, S. I., Nixon, M. R., Dolecek, C., James, S. L., Mokdad, A. H., Abebe, G., Ahmadian, E., Alahdab, F., Alemnew, B. T. T., Alipour, V., Allah Bakeshei, F., Animut, M. D., Ansari, F., Arabloo, J., … Crump, J. A. (2019). The global burden of non-typhoidal salmonella invasive disease: a systematic analysis for the Global Burden of Disease Study 2017. The Lancet Infectious Diseases, 19(12), 1312–1324. https://doi.org/10.1016/S1473-3099(19)30418-9
Tarazi, Y. H., al Dwekat, A. F., & Ismail, Z. B. (2021). Molecular characterization of Salmonella spp. isolates from river and dam water, irrigated vegetables, livestock, and poultry manures in Jordan. Veterinary World, 14(3), 813–819. https://doi.org/10.14202/vetworld.2021.813-819
Ta?kale Karatu?, N., Yüksel, F. N., Akçelik, N., & Akçelik, M. (2018). Genetic diversity of food originated Salmonella isolates. Biotechnology & Biotechnological Equipment, 32(3), 638–645. https://doi.org/10.1080/13102818.2018.1451779
Thung, T. Y., Radu, S., Mahyudin, N. A., Rukayadi, Y., Zakaria, Z., Mazlan, N., Tan, B. H., Lee, E., Yeoh, S. L., Chin, Y. Z., Tan, C. W., Kuan, C. H., Basri, D. F., & Wan Mohamed Radzi, C. W. J. (2018). Prevalence, Virulence Genes and Antimicrobial Resistance Profiles of Salmonella Serovars from Retail Beef in Selangor, Malaysia. Frontiers in Microbiology, 8. https://doi.org/10.3389/fmicb.2017.02697
v. T. Nair, D., Venkitanarayanan, K., & Kollanoor Johny, A. (2018). Antibiotic-Resistant Salmonella in the Food Supply and the Potential Role of Antibiotic Alternatives for Control. Foods, 7(10), 167. https://doi.org/10.3390/foods7100167
Vollaard, A. M., Ali, S., Widjaja, S., Asten, H. A. G. H. van, Visser, L. G., Surjadi, C., & van Dissel, J. T. (2005). Identification of typhoid fever and paratyphoid fever cases at presentation in outpatient clinics in Jakarta, Indonesia. Transactions of the Royal Society of Tropical Medicine and Hygiene, 99(6), 440–450. https://doi.org/10.1016/j.trstmh.2004.09.012
WHO. (2011). Guidelines for the Management of Typhoid Fever.
WHO?: Standards, S. (2018). Typhoid and other invasive salmonellosis Vaccine-Preventable Diseases.
Zha, L., Garrett, S., & Sun, J. (2019). Salmonella Infection in Chronic Inflammation and Gastrointestinal Cancer. Diseases, 7(1), 28. https://doi.org/10.3390/diseases7010028
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