Characteristics and activity of chitosan from mud crab shells on acne bacteria: Staphylococcus aureus, S. epidermidis and Propionibacterium acnes
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Abstract. Luthfiyana N, Bija S, Anwar E, Laksmitawati DE, Risalinda GL. 2022. Characteristics and activity of chitosan from mud crab shells on acne bacteria: Staphylococcus aureus, S. epidermidis and Propionibacterium acnes. Biodiversitas 23: 6645-6651. Chitosan is a product of chitin deacetylation, which has the potential to act as acne bacteria. The study aimed to determine the characteristics and activity of chitosan from mud crab shells on acne bacteria, i.e., Staphylococcus aureus FNCC 0047, Staphylococcus epidermidis FNCC 0048 and Propionibacterium acnes ATCC 11827. The research used a descriptive method for analyzing the quality of chitosan and antibacterial activity using analysis of variants at a 95% confidence interval with one factor, while the addition of chitosan as much as 0.25%; 0.5%; 0.75%; and 1% with three replications. The results showed that the value of deacetylation degree (DD) was 76%. The morphology of chitosan shows irregular, wavy, porous shapes and contains several elements: C, O, Ca, and Al. The antibacterial activity of chitosan at concentrations of 0.25%, 0.5%, 0.75%, and 1% showed an inhibition zone of S. aureus (9.5±0.71 mm, 10±0 mm, 11±0 mm, 13.5±0.71 mm), S. epidermidis (9±0 mm, 10±0 mm, 11±0 mm, 12.5±0.71 mm), and P. acnes (28.5±0.7 mm, 28.5±0.7 mm, 31±5.56 mm, 34.5±6.36 mm). The concentration of 1% gives the effectiveness in inhibiting the growth of acne bacteria. This study concludes that chitosan from mud crab shells could be the potential as an anti-acne.
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