Fungal community associated with mixed infection of anthracnose and stem end rot diseases in Chokanan Mango

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ANI WIDIASTUTI
SURYANTI
ALVINA CLARA GIOVANNI
ISLAMINATI ANNA SANTIKA
NIKEN RASMI PARAMITA

Abstract

Abstract. Widiastuti A, Suryanti, Giovanni AC, Santika IA, Paramita NR. 2023. Fungal community associated with mixed infection of anthracnose and stem end rot diseases in Chokanan Mango. Biodiversitas 24: 2163-2170. Anthracnose and stem end rot are the top two prevalent diseases causing losses in mango fruit worldwide. Both diseases often appear together in ripened fruits. The aim of this research was to evaluate the fungal community associated with mixed infection of anthracnose and stem end rot diseases in Chokanan mango based on metagenomics analysis through amplicon targeted next generation sequencing. The results showed that approximately 152,000 sequences were observed. The average total tags from the observed OTUs number were 146,485, of which 126,230 were taxon tags. The ten most common occurring fungal genera were Colletotrichum, Penicillium, Diaporhte, Purpureocillium, Aspergillus, Cunninghamella, Neofusicoccum, Mortierella, Rhizopus, and Kazachstania. Of these, genus Colletotrichum showed 77% dominance of based on Krona display value. Based on the number of OTUs, seven species, such as Colletotrichum gloeosporioides (Penz.) Penz. & Sacc., Penicillium simplicissimum (Oudem.) Thom, Neofusicoccum cordaticola Pavlic, Slippers & M.J.Wingf., Diaporthe arengae R.R.Gomes, Glienke & Crous, Purpureocillium lilacinum (Thom) Luangsa-ard, Houbraken, Hywel-Jones & Samson, Alternaria alternata (Fr.) Keissl., and Fusarium oxysporum Schltdl., showed dominance in mixed infection. These findings reveal the major status of post-harvest pathogens in mango fruits that should be controlled. This is the first report on N. cordaticola and D. arengae associated with mixed infection of anthracnose and stem and rot disease on post-harvest mango in Indonesia.

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References
Aloi F, Riolo M, Parlascino R, Pane A, Cacciola SO. 2021. Bot gummosis of lemon (citrus × limon) caused by Neofusicoccum parvum. J Fungi 7(4) 294: 1-16. DOI: 10.3390/jof7040294.
Alvarez LV, Hattori Y, Deocaris CC, Mapanao CP, Bautista AB, Cano MJB, Naito K, Kitabata S, Motohashi K, Nakashima C. 2020. Colletotrichum asianum causes anthracnose in Philippine mango cv. Carabao. Australas Plant Dis Notes 15(13): 1-5. DOI: 10.1007/s13314-020-00384-x.
An GH, Cho JH, Kim OT, and Han JG. 2021. Metagenomic analysis of bacterial and fungal communities inhabiting shiro dominant soils of two production regions of Tricholoma Matsutake S. Ito and S. Imai in Korea. Forests 12(6):1-16. DOI: 10.3390/f12060758.
Azhar HM, Asrul SM, Johari S, Malik, TMA. 2013. Variation study on morphological characters among Magnifera indica L. ‘Chokanan’ for development of superior mango clone. Acta Hortic 1012: 305-313. DOI: 10.17660/ActaHortic.2013.1012.38.
Bejo SK, Kamaruddin S. 2014. Determination of Chokanan mango sweetness (Mangifera indica) using non-destructive image processing technique. Australian J Crop Sci AJCS 8(4): 475-480. http://www.cropj.com/bejo_8_4_2014_475_480.pdf.
Benatar GV, Wibowo A, Suryanti. 2021. First report of Colletotrichum asianum associated with mango fruit anthracnose in Indonesia. J Crop Prot 141(105432): 1-6. DOI: 10.1016/j.cropro.2020.105432.
Bodor A, Bounedjoum N, Vincze GE, Kis, AE, Laczi K, Bende G, Szilagyi A, Kovacs T, Perei K, Rakhely G. Challenges of unculturable bacteria: environmental perspectives. Rev Environ Sci Biotechnol 19: 1–22 . DOI: 10.1007/s11157-020-09522-4.
Bokulich NA, Subramanian S, Faith JJ, Gevers D, Gordon JI, Knight R, Mills DA, Caporaso JG. 2013. Quality-filtering vastly improves diversity estimates from Illumina amplicon sequencing. Nat Methods 10(1): 57-59. DOI: 10.1038/nmeth.2276.
BPS (Badan Pusat Statistik). 2023. Produksi tanaman buah-buahan 2021. https://www.bps.go.id/indicator/55/62/1/produksi-tanaman-buah-buahan.html. [Accessed on 17 February 2023].
CABI. 2019. Invasive species compendium. https://www.cabi.org/isc/datasheet/16983.
Caporaso JG, Kuczynski J, Stombaugh J, Bittinger K, Bushman FD, Costello EK, Fierer N, Peña AG, Goodrich JK, Gordon JI, Huttley GA, Kelley ST, Knights D, Koenig JE, Ley RE, Lozupone CA, McDonald D, Muegge BD, Pirrung M, Reeder J, Sevinsky JR, Turnbaugh PJ, Walters WA, Widmann J, Yatsunenko T, Zaneveld J, Knight R. 2010. QIIME allows analysis of high-throughput community sequencing data. Nat Methods 7(5): 335-336. DOI: 10.1038/nmeth.f.303.
Deng ZS, Liu XD, Zhang BC, Jiao S, Qi XY, Sun ZH, He XL, Liu YZ, Li J, Chen KK, Lin ZX, Jiang YY. 2019. The root endophytic fungi community structure of Pennisetum sinese from four representative provinces in China. Microorganisms 7(9) 332:1-13. DOI: 10.3390/microorganisms7090332.
Diedhiou PM, Mbaye N, Drame A, Samb PI. 2007. Alteration of post-harvest diseases of mango (Mangifera indica) through production practices and climatic factors. Afr J Biotechol 6(9): 1087-1094. DOI: 10.1653/024.100.0209.
Diskin S, Oleg F, Dalia M, Samir D, Dov P, Noam A. 2017. Microbiome alterations are correlated with occurrence of postharvest stem-end rot in mango Fruit. Pythobiome J 1: 117-127. DOI:10.1094/PBIOMES-05-17-0022-R.
Edgar RC, 2004. Muscle: Multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 32(5): 1792-1797. DOI: 10.1093/nar/gkh34.
Edgar RC, Hass BJ, Clemente JC, Quince C, Knight R. 2011. Uchime improves sensitivity and speed of chimera detection. Bioinformatics 27(16): 2194-2200. DOI: 10.1093/bioinformatics/btr381.
Edgar RC, 2013. Uparse: highly accurate OTU sequences from microbial amplicon reads. Nat Methods 10(10): 996-998. DOI: 10.1038/nmeth.2604.
Effendi Y, Pambudi A, Pancoro A. 2019. Metagenomic analysis of Fusariumo xysporum f.sp. cubense-infected soil in banana plantation, Sukabumi, Indonesia. Biodiversitas 20(7): 1939-1945. DOI: 10.13057/biodiv/d200721.
Fricke WF, Cebula TA, Ravel J. 2011. Chapter 28: Genomics.In: Budowle B, Steven E, Roger G, Breeze, Paul S K, Stephen A M (eds) Microbial forensics (2nd edition): 479-492. Academic Press. United States. DOI: 10.1016/b978-0-12-382006-8.00028-1.
Galsurker O, Sonia D, Dalia M, Oleg F, and Noam A. 2018. Fruit stem-end rot. Horticulturae 4(4):1-16. DOI: 10.3390/horticulturae4040050.
Galsurker O, Diskin S, Duanis-Assaf D, Doron-Faigenboim A, Maurer D, Feygenberg O, Alkan N. 2020. Harvesting mango fruit with a short stem-end altered endophytic microbiome and reduce stem-end rot. Microorganisms 8(4)558:1-18. DOI: 10.3390/microorganisms8040558.
Haas BJ, Gevers D, Earl AM, Feldgarden M, Ward DV, Giannoukos G, Ciulla D, Tabbaa D, Highlander SK, Sodergren E, Methé B, DeSantis TZ; Human Microbiome Consortium, Petrosino JF, Knight R, Birren BW. 2011. Chimeric 16S rRNA sequence formation and detection in Sanger and 454-pyrosequenced PCR amplicons. Genome Res 21(3): 494-504. DOI: 10.1101/gr.112730.110.
Henuk JBD, Sinaga MS, Hidayat SH. 2017. Morphological and molecular identificationof fungal pathogens causing gummosis disease of Citrus spp. in Indonesia. Biodiversitas 18(3): 1100-1108. DOI: 10.13057/biodiv/d180330.
Kellenberger E. 2001. Exploring the unknown. The silent revolution of microbiology. EMBO Rep 2(1):5-7. DOI: 10.1093/embo-reports/kve014.
Kõljalg U, Nilsson RH, Abarenkov K, Tedersoo L, Taylor AF, Bahram M, Bates ST, Bruns TD, Bengtsson-Palme J, Callaghan TM, Douglas B, Drenkhan T, Eberhardt U, Dueñas M, Grebenc T, Griffith GW, Hartmann M, Kirk PM, Kohout P, Larsson E, Lindahl BD, Lücking R, Martín MP, Matheny PB, Nguyen NH, Niskanen T, Oja J, Peay KG, Peintner U, Peterson M, Põldmaa K, Saag L, Saar I, Schüßler A, Scott JA, Senés C, Smith ME, Suija A, Taylor DL, Telleria MT, Weiss M, Larsson KH. 2013. Towards a unified paradigm for sequence-based identification of fungi. Mol Ecol 22(21): 5271-5277. DOI: 10.1111/mec.12481.
Li Q, Bu J, Shu J, Yu Z, Tang L, Huang S, Guo T, Mo J, Luo S, Solangi GS, Hsiang T. 2019. Colletotrichum species associated with mango in southern China. Sci Rep 9(1):18891. DOI: 10.1038/s41598-019-54809-4.
Lim L, Mohd MH, Zakaria L. 2019. Identification and pathogenicity of Diaporthe species associated with stem-end rot of mango (Mangifera indica L.). Eur J Plant Pathol 155: 687–696. DOI: 10.1007/s10658-019-01800-4.
Lu M, Yang Z, Ma L, Li Q, Huang S, Tang L, Guo T, Mo J, Hsianget T. 2022. First report of postharvest anthracnose of mango fruit caused by Colletotrichum plurivorum in China. J Plant Pathol 104: 839–840. DOI: 10.1007/s42161-022-01047-9.
Lundberg DS, Yourstone S, Mieczkowski P, Jones CD, Dangl JL. 2013. Practical innovations for high-throughput amplicon sequencing. Nat Methods 10(10): 999-1002. DOI: 10.1038/nmeth.2634.
Marques MW, Lima NB, de Morais Jr MA, Michereff SJ, Phillips AJL, Câmara MPS. 2013. Botryosphaeria, Neofusicoccum, Neoscytalidium and Pseudofusicoccum species associated with mango in Brazil. Fungal Divers 61:195–208. DOI: 10.1007/s13225-013-0258-1
Mago? T, Salzberg SL. 2011. FLASH: fast length adjustment of short reads to improve genome assemblies. Bioinformatics 27(21): 2957-63. DOI: 10.1093/bioinformatics/btr507.
Navitasari L, Joko T, Murti RH, Arwiyanto. T. 2015. Rhizobacterial community structure in grafted tomato plants infected by Ralstonia solanacearum. Biodiversitas 21(10): 4888-4895. DOI: 10.13057/biodiv/d211055
Hossain MM, Sultana F, Kubota M, Koyama H, Hyakumachi M. 2007. The plant growth-promoting fungus Penicillium simplicissimum GP17-2 induces resistance in Arabidopsis thaliana by activation of multiple defense signals. Plant Cell Physiol 48(12): 1724–1736. Doi: 10.1093/pcp/pcm144.
Pinto C, Pinho D, Sousa S, Pinheiro M, Egas C, Gomes AC. 2014. Unravelling the diversity of grapevine microbiome. PLoS ONE 9(1): 1-12. Doi: 10.1371/journal.pone.0085622.
Puig AS, Winterstein MC. 2021 Neofusicoccum batangarum Causing Dieback of Mango (Mangifera indica) in Florida. Agriculture 11(9) 853:1-9. DOI: 10.3390/agriculture11090853.
Prakoso AB, Suryanti, Widiastuti A. 2019. Molecular detection of Colletotrichum spp. on postharvest commodities of horticulture in Central Java and Yogyakarta, Indonesia. AIP Conference Proceedings on Postharvest Commodities of Horticulture in Central Java and Yogyakarta, Indonesia 2099: 020017: 1-9. DOI: 10.1063/1.5098422.
Sakalidis ML, Slippers B, Wingfield BD, Hardy GESJ, Burgess TI. 2013. The challenge of understanding the origin, pathways and extent of fungal invasions: global populations of the Neofusicoccum parvum-N. ribis species complex. Diversity Distrib 19: 873-883. https://doi.org/10.1111/ddi.12030
Sarsono Y, Sugiprihatini D, Murdiati T, Maylandari ME, Febbiyanti TR. 2020. Characterization of Fusicoccum (Crous. S and Wingf. M.J) isolates on rubber (Hevea brasiliensis) through morphologically and polymerase chain reaction technique. Indonesian J Nat Rubb Res 38(1): 27- 36. DOI: 10.22302/ppk.jpk.v38i1.665.
Satitmunnaithum J, Kitazawa H, Arofatullah NA, Widiastuti A, Kharisma AD, Yamane K, Tanabata S, Sato T. 2022. Microbial population size and strawberry fruit firmness after drop shock-induced mechanical damage. Postharvest Biol Technol 92(112008): 1-8. DOI: 10.1016/j.postharvbio.2022.112008.
Sharma A, Sharma IM, Sharma M, Sharma K, Sharma A. 2021. Effectiveness of fungal, bacterial and yeast antagonists for management of mango anthracnose (Colletotrichum gloeosporioides). Egypt J Biol Pest Control 31(135): 1-11. DOI: 10.1186/s41938-021-00480-9.
Serrato-Diaz LM, Rivera-Vargas LI, French-Monar RD. 2014. First report of Diaporthe pseudomangiferae causing inflorescence rot, rachis canker, and flower abortion of mango. Plant Dis 98(7): 1004-1004. DOI: 10.1094/PDIS-12-13-1223-PDN.
Taylor MW, Tsai P, Anfang N, Ross H A, Goddard MR. 2014. Pyrosequencing reveals regional differences in fruit?associated fungal communities. Environ Microbiol 16: 2848-2858. DOI: 10.1111/1462-2920.12456. .
Tovar-Pedraza JM, Mora-Aguilera JA, Nava-Díaz C, Lima NB, Michereff SJ, Sandoval-Islas JS, Câmara MPS, Téliz-Ortiz D, Leyva-Mir SG. 2020. Distribution and pathogenicity of Colletotrichum species associated with mango anthracnose in Mexico. Plant Dis 104: 137–146. DOI: 10.1094/PDIS-01-19-0178-RE.
Tucker CM, Cadotte MW, Carvalho SB, Davies TJ, Ferrier S, Fritz SA, Grenyer R, Helmus MR, Jin LS, Mooers AO, Pavoine S, Purschke O, Redding DW, Rosauer DF, Winter M, Mazel F. 2017. A guide to phylogenetic metrics for conservation, community ecology and macroecology. Biol Rev Camb Philos Soc 92(2): 698-715. DOI: 10.1111/brv.12252.
Widiastuti A. 2013. Fruit rot disease caused by Lasiodiplodia spp. on several postharvest fruits in Indonesia. In: Proceeding of the 1st International Conference on Horticultural Crops. Ministry of Agriculture, Yogyakarta, 2–4 Oct 2013; Yogyakarta, Indonesia: Ministry of Agriculture.
Widiastuti A, Ningtyas OH, Priyatmojo A. 2015. Identification of fungus causing postharvest disease on several fruits in Yogyakarta. J Fitopatol Indon 11(3): 91-96. DOI: 10.14692/jfi.11.3.91.

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