Characterization of morphological, micromorphological, anatomical structures and matK gene-based identification of aromatic litsea (Litsea cubeba)
##plugins.themes.bootstrap3.article.sidebar##
Issue
Section
Articles
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
##plugins.themes.bootstrap3.article.main##
Abstract
Abstract. Aini IN, Wisanti. 2023. Characterization of morphological, micromorphological, anatomical structures and matK gene-based identification of aromatic litsea (Litsea cubeba). Biodiversitas 24: 4557-4567. Aromatic litsea or krangean (Litsea cubeba (Lour.) Persoon) has potential as a drug. L. cubeba's status based on the IUCN red list is the least concern. This research aimed to (i) characterize morphology, micromorphology, and anatomy; (ii) indetify L. cubeba species based on DNA barcodes. The research samples were collected in the Mt. Anjasmoro, East Java, Indonesia at three different altitudes. Data in the form of various characters were analyzed using the one-way ANOVA method to determine the significance of the difference. The statistical test analysis of the quantitative character of L. cubeba showed a significant difference between heights (? = 0.05). The results showed that the characterization of L. cubeba includes (i) morphology: structure of lenticels, color, and substance that coats the lower surface of leaves, type of flower, fruit taste, and aroma; (ii) micromorphology: glands lining the abaxial surface of the lamina, trichomes on both surfaces of the lamina, structure of abaxial epidermal cells, the shape of pollen, shape and distribution of exin (iii) anatomy: cuticle thickness, epidermal cell shape, and presence of secretory cells in the lamina. The molecular identification in the form of L. cubeba barcode DNA based on the matK gene marker matched the Litsea cubeba sequence in NCBI (AB259073.1).
##plugins.themes.bootstrap3.article.details##
References
Agashe SN, Caulton E. 2009. Pollen and spores: applications with special emphasis on aerobiology and allergy. Science Publishers, United States of America. DOI: 10.1201/9780429063985
Aprianty NMD, Kriswiyanti E. 2008. Studi variasi ukuran polen kembang sepatu (Hibiscus rosa-sinensis L) dengan warna bunga berbeda. Jurnal Biologi 12 (1): 14-18.
Azah MAN, Susiarti S. 2021. Litsea cubeba (Plant Resources of South-East Asia). (Online) PlantUse English (https://uses.plantnet-https://uses.plantnet-project.org/e/index.php?title=Litsea_cubeba_(PROSEA)&oldid=331638 accessed at 13:25, 23th Februari 2023).
Backer CA., Brink RCBVD. 1963. Flora of Java (Spermatophytes Only). N.V.P. Noordhoff, Netherlands.
Badan POM RI. 2010. Acuan sediaan herbal Vol 5 edisi pertama. Direktorat Obat Asli Indonesia, Jakarta.
Bermawie N. 2005. Buku Pedoman Pengelolaan Plasma Nutfah Perkebunan. Pusat Penelitian dan Pengembangan Perkebunan, Bogor.
Chen YC, Li Z, Zhao YX, Gao M, Wang JY, Liu KW, Wang X, Wu LW, Jiao YL, Xu ZL, He WG, Zhang QY, Liang CK, Hsiao YY, Zhang DY, Lan SR, Huang L, Xu W, Tsai WC, Liu ZJ, Peer YVD, Wang YD. 2020. The Litsea genome and the evolution of the Laurel family. Nature Communications 11 (1675): 1-14. DOI: 10.1038/s41467-020-15493-5
Chen Y, Wang Y, Han X, Si L, Wu Q, Lin L. 2013. Biology and chemistry of Litsea cubeba, a promising industrial tree in China. Journal of Essential Oil Research 25 (2): 103–111. DOI: 10.1080/10412905.2012.751559
Dereeper A, Guignon S, Blanc, VG, Audic S, Buffet S, Chevenet F, Dufayard JF, Guindon S, Lefort V, Lescot M, Claverie JM, Gascuel O. 2008. Phylogeny. fr: robust phylogenetic analysis for the non-specialist. Nucleic Acids Research 1, 36. DOI: 10.1093/nar/gkn180
Dorly, Ningrum RK, Suryantari NK, Anindita FLR. 2016. Studi anatomi daun dari tiga anggota suku Malvaceae di kawasan Waduk Jatiluhur. Proceeding Seminar Nasional XIII Pendidikan Biologi, FKIP UNS 13 (1): 611-618.
Fatonah S, Asih D, Mulyanti D, Irianai D. 2013. Penentuan waktu pembukaan stomata pada gulma Melastoma malabathricum L. di Perkebunan Gambir Kampar. Jurnal Biospesies 6 (2): 15-22.
Fijridiyanto IA, Murakami N. (2016). Litsea cubeba chloroplast matK gene for maturase, complete cds, specimen_voucher: MAK:VII.C. 50a. NCBI www.ncbi.nlm.nih.gov/nuccore/AB259073.1
Gang Z, Liu B, Rohwer JG, Ferguson DK, Yang Y. 2021. Leaf epidermal micromorphology defning the clades in Cinnamomum (Lauraceae). PhytoKeys 182: 125–148. DOI: 10.3897/phytokeys.182.67289
Geng-guo T, Chih-bei S. 1995. Pollen morphology of the family Lauraceae in China. Plant Syst Evol, 33 (2): 161-171.
Gonçalves RDA, Pinheiro AB, Oliveira MAD, Nascimento RTD, Rosalem PF, Garcia VL, Martins AR. 2018. Anatomical characters and chemical profile of leaves of three species in Lauraceae family. Revista Brasileira de Farmacognosia 28: 1-8. DOI: 10.1016/j.bjp.2017.11.008
Gurcharan S. 2005. Plant Systematics an Integrated Approach. Science Publishers, New Hampshire.
Hari N, Priya C, Krishnapriya S, Amrutha L, Praseetha K. 2021. A Monogram study on anatomical characteristics in Litsea quinqueflora (Dennst.) Suresh. 1-4.
Heryati Y, Mindawati N, Kosasih AS. 2009. Prospek pengembangan lemo (Litsea cubeba L. Persoon) di Indonesia. Teknologi Hutan Tanaman, 2 (1): 9–17.
Huang XW, Feng YC, Huang Y. 2013. Potential cosmetic application of essential oil extracted from Litsea cubeba fruits from China. Journal of Essential Oil Research 25: 112-119. DOI: https://doi.org/10.1080/10412905.2012.755479
Jiménez-Pérez NDC, Lorea-Hernández FG. 2009. Identity and delimitation of the American species of Litsea lam. (Lauraceae): a morphological approach. Plant Syst Evol 283: 19-32. DOI: 10.1007/s00606-009-0218-0
Kalqutny SH, Pakki S, Muis A. 2020. Potensi pemanfaatan teknik molekuler berbasis DNA dalam penelitian penyakit bulai pada jagung. Agrosainstek 4 (1): 17–27. DOI: 10.33019/agrosainstek.v4i1.107
Kamle M, Mahato DK, Lee KE, Bajpai VK, Gajurel PR, Gu KS, Kumar P. 2019. Ethnopharmacological properties and medicinal uses of Litsea cubeba. Plants 8 (6): 150–163. DOI: 10.3390/plants8060150
Kayang H, Kharbuli B, Syeim D. 2009. Litsea cubeba Pers.-an untapped economic plant species of Meghalaya. natural product radiance. A Bimontly Journal on Natural Product 8 (1): 1–2.
Kok DR. 2021. Litsea cubeba. The IUCN Red List of Threatened Species 2021: e.T150217538A150219934.
Kunuku VR, Aluri JSR. 2019. Pollination ecology of Litsea glutinosa (Lour.) C.B. Robinson (Lauraceae): a commercially and medicinally important semi-evergreen tree species. SJST 41 (1): 31-36. DOI:10.14456/sjst-psu.2019.4
Kunuku VR, Balaramaswamy YP, Aluri JSR. 2019. Pollination ecology of Litsea deccanensis Gamble (Lauraceae), a commercially and medicinally important semi-evergreen tree species. Species 20: 1-12.
Liu ZF, Ci XQ, Li L, Li HW, Conran JG, Li J. 2017. DNA barcoding evaluation and implications for phylogenetic relationships in Lauraceae from China. PLoS one 12 (4): 1-20. DOI: 10.1371/journal.pone.0175788
Mao AA, Wetten A, Fay MF, Caligari PDS. 2000. In vitro propagation of Litsea cubeba (Lours.) Pers., a multipurpose tree. Plant Cell Reports 19: 263-267. DOI: 10.1007/s002999900099
Marina E, Manurung H, Nugroho RA. 2015. Uji fitokimia dan antibakteri ekstrak etanol daun balangla (Litsea cubeba ( Lour .) Pers.) terhadap bakteri Stapylococcus aureus dan Escherichia coli. Proceeding of the Seminar on Science and Technology FMIPA, Mulawarman University, Samarinda 1(1), 1–10. [Indonesian]
Marzuki I, Uluputty MR, Aziz SA, Surahman M. 2008. Karakterisasi morfoekotipe dan proksimat pala banda (Myristica fragrans Houtt). Buletin Agron 36 (2): 146-152. DOI: 10.24831/jai.v36i2.20505
Merwe VDJJM, Wyk AEV, Kok PDF. 1990. Pollen types in the Lauraceae. Grana 29 (3): 185-196. DOI: 10.1080/00173139009427751
Nishida S, Kok DR, Yang Y. 2016. Cuticular features of Cryptocarya (Lauraceae) from Peninsular Malaysia, Thailand and Indo–China and its taxonomic implications. Phytotaxa 244 (1): 26–44. DOI: 10.11646/phytotaxa.244.1.2
Othman OM, Hamad RM. 2022. Pollen micromorphological study of ten genera of Brassicaceae in West Iraq Desert. IOP Conf. Ser: Earth Environ. Sci 1060: 1-11. DOI: 10.1088/1755-1315/1060/1/012103
Pei N, Lian JY, Erickson DL, Swenson NG, Kress WJ, Ye WH, Ge XJ. 2011. Exploring tree-habitat associations in a Chinese subtropical forest plot using a molecular phylogeny generated from DNA barcode loci. PLos ONE 6 (6). DOI: 10.1371/journal.pone.0021273
Prawasti TS, Sulistyaningsing YC, Dorly, Juliandi B, Juliarni. 2014. Penuntun Praktikum Mikroteknik. Institut Pertanian Bogor, Bogor.
Pudjiono S, Septina S. 2008. Morfologi tanaman hibrid murbei di Purwobinangun Yogyakarta. Jurnal Pemuliaan tanaman Hutan 2 (1): 163-171. DOI: 10.20886/jpth.2008.2.1.163-171
Putri KP, Syamsyu D, Kurniaty R. 2015. Budidaya kilemo (Litsea cubeba) untuk mendukung kelestarian tanaman dataran tinggi penghasil atsiri. Proceedings of Seminar Nasional Masyarakat Biodiversitas Indonesia, 1 September 2015, 1 (6): 1487–1491. [Indonesian] DOI: 10.13057/psnmbi/m010639
Rasyid M, Irawati MH, Saptasari M. 2017. Anatomi daun Ficus racemosa L. (biraeng) dan potensinya di Taman Nasional Bantimurung Bulusaraung. Jurnal Pendidikan: Teori, Penelitian, dan Pengembangan 2 (6) 861-866.
Retamales HA, Scharaschkin T. 2014. A staining protocol for identifying secondary compounds in Myrtaceae. Appl Plant Sci 2 (10): 1-8. DOI: 10.3732/apps.1400063.
Simpson MG. 1953. Plant systematics. Elsevier, British.
Steenis CGGJV. 2010. Flora Pegunungan Jawa. (Jenny A. Kartawinata). LIPI Press: Bogor.
Sulassih, Sobir, Santosa E. (2013). Phylogenetic analysis of mangosteen (Garcinia mangostana L.) and its relatives based on morphological and Inter Simple Sequence Repeat (ISSR) markers. SABRAO Journal of Breeding and Genetics 45 (3): 478-490.
Sundari T, Atmaja RP. 2011. Bentuk sel epidermis, tipe stomata dan indeks stomata 5 genotipe kedelai pada tingkat naungan berbeda. Jurnal Biologi Indonesia 7 (1): 67-79. DOI: 10.14203/jbi.v7i1.3129
Supriningrum R, Hendra M, Misak H. 2016. Uji pendahuluan daun tenem Litsea cubeba (Lour.) Pers. Jurnal Ilmiah Manuntung 2 (1): 28-31. DOI: 10.51352/jim.v2i1.42
Trofimov D, Rohwer JG. 2018. Epidermal features allowing identifcation of evolutionary lineages in the Ocotea complex (Lauraceae). Perspectives in Plant Ecology, Evolution and Systematics 31: 17-35. DOI: 10.1016/j.ppees.2017.12.003
Vasco A, Thadeo M, Conover M, Daly DC. 2014. Preparation of samples for leaf architecture studies, a method for mounting cleared leaves. Appl Plant Sci 2 (9): 1-4. DOI: 10.3732/apps.1400038.
Wang YF, Chen SH. 2001. Pollen flora of Yuenyang Lake Natura Preserve, Taiwan (II). Taiwania 46 (2): 167-191. DOI: 10.6165/tai.2002.47(2).129
Werff HVD. 2019. Alseodaphnopsis (Lauraceae) revisited. Blumea 64: 186-189. DOI: 10.3767/blumea.2019.64.02.10
Wulansari TYI, Agustiani EL, Sunaryo, Tihurua, EF, Widoyanti. 2020. Struktur anatomi daun sebagai bukti dalam pembatasan takson tumbuhan berbunga: studi kasus 12 suku tumbuhan berbunga Indonesia. Buletin Kebun Raya 23 (2): 146-161. DOI: 10.14203/bkr.v23i2.266
Yang Z, Tan C, Wei YM, Rohwer JG, Liu B, Yang Y. 2022. Floral morphology and phenology of Sassafras tzumu (Lauraceae). BMC Plant Biol 22 (327): 1-12. DOI: 10.1186/s12870-022-03714-6
Zhang Y, Tian Y, Tng DYP, Zhou J, Zhang Y, Wang Z, Li P, Wang Z. 2021. Comparative chloroplast genomics of Litsea lam. (Lauraceae) and its phylogenetic implications. Forests 12 (6): 1-16. DOI: 10.3390/f12060744
Zhao O, Zhou JW, Ban DM. 2010. Analysis of volatile oil from different parts of Litsea cubeba. Journal of Chinese Medicinal Materials 33 (9): 1417-1419. PMID: 21243772.
Most read articles by the same author(s)
- WISANTI, DURAN COREBIMA ALOYSIUS, SITI ZUBAIDAH, SRI RAHAYU LESTARI, Variation in morphological characters of Marsilea crenata living in floating aquatic, emergent aquatic, and terrestrial habitats , Biodiversitas Journal of Biological Diversity: Vol. 22 No. 7 (2021)